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The map label for this gene is pyrB [H]

Identifier: 125623736

GI number: 125623736

Start: 863245

End: 864177

Strand: Direct

Name: pyrB [H]

Synonym: llmg_0893

Alternate gene names: 125623736

Gene position: 863245-864177 (Clockwise)

Preceding gene: 125623735

Following gene: 125623737

Centisome position: 34.13

GC content: 38.91

Gene sequence:

>933_bases
ATGTCAGTAAAAAATGGATTAGTTCAACTCGAAAACCTTACAAGCATGGAAAACTTGAGCATTGATGAAGTCATGGGACT
TATTAAAAGAGCCTCAGCTTTCAAAGCAGGAAAAGCAGACTTCGCTTTAGATGAGCAAACTTTCGCTTCAAATCTATTCT
TTGAAAATTCTACAAGAACACATCACAGTTTTCATATTGCCGAACGTAAACTAGGACTTGATGTTCTTGAATTTGATGCA
CAAGCCAGTTCAATCAGTAAAGGTGAAACACTATACGATACGGTTTTAACGCTTGATGCACTTGGTGTTGACATCTGCGT
CATTCGCTCTGGAGTAGAACATTACTACGAAGAATTAGTGAATTCAGACAATATCCACTGTGCCATTGTCAATGGTGGAG
ACGGTTCAGGACAACATCCAAGTCAATGTTTACTTGACTTGATGACTATTTATGAAGAATTTGGCAAATTTGAAGGACTA
AAAATTGCAATTTCTGGAGATTTAACACACTCACGTGTGGCTAAATCAAATATGATGATGTTGCAAAAACTTGGGGCTAG
ACTTTATTTCACTGGTCCAGCAGCATGGTACAGTGAAGAATTTGACGATTACGGGCATTATGCCAACCTAGACCGTATTT
TACCAGAACTTGATGTTCATATGCTCTTACGCGTGCAACATGAACGTCATGACAGTGGTGAAAGTTTTTCAAAAGAAGGT
TATCACACTCATTTTGGACTGACGCAAGAACGAGCAAAAATGTTGAAACCAACAGCAATTATTATGCACCCAGCTCCTGT
TAATCGTGATGTTGAAATTGCTGACAGCCTCGTTGAAAGTCCACAAAGTCGCATTGTTCAACAAATGAGCAATGGTGTTT
ACACACGTATGGCCATTCTTGAAGCCATTCTTGCTGGAAAAAAAGCGAAGTAA

Upstream 100 bases:

>100_bases
TCGTTAGTAAATAAAAATTTTGTCAGTTTACTGAAGAAATAAATATATTGTCAAAAAACTGTTGTGACAGAGAGCACACA
GTCAGGGTAAAGGAGACCTT

Downstream 100 bases:

>100_bases
GTTGAAATTGATGACAATAGGACAAAAAGAAATATTCAAAACTAAATTGTTCTGTTTTTAAAGCAAAGTTTAAAGGAAAT
AATTATTATGAGCAAAAGAC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSVKNGLVQLENLTSMENLSIDEVMGLIKRASAFKAGKADFALDEQTFASNLFFENSTRTHHSFHIAERKLGLDVLEFDA
QASSISKGETLYDTVLTLDALGVDICVIRSGVEHYYEELVNSDNIHCAIVNGGDGSGQHPSQCLLDLMTIYEEFGKFEGL
KIAISGDLTHSRVAKSNMMMLQKLGARLYFTGPAAWYSEEFDDYGHYANLDRILPELDVHMLLRVQHERHDSGESFSKEG
YHTHFGLTQERAKMLKPTAIIMHPAPVNRDVEIADSLVESPQSRIVQQMSNGVYTRMAILEAILAGKKAK

Sequences:

>Translated_310_residues
MSVKNGLVQLENLTSMENLSIDEVMGLIKRASAFKAGKADFALDEQTFASNLFFENSTRTHHSFHIAERKLGLDVLEFDA
QASSISKGETLYDTVLTLDALGVDICVIRSGVEHYYEELVNSDNIHCAIVNGGDGSGQHPSQCLLDLMTIYEEFGKFEGL
KIAISGDLTHSRVAKSNMMMLQKLGARLYFTGPAAWYSEEFDDYGHYANLDRILPELDVHMLLRVQHERHDSGESFSKEG
YHTHFGLTQERAKMLKPTAIIMHPAPVNRDVEIADSLVESPQSRIVQQMSNGVYTRMAILEAILAGKKAK
>Mature_309_residues
SVKNGLVQLENLTSMENLSIDEVMGLIKRASAFKAGKADFALDEQTFASNLFFENSTRTHHSFHIAERKLGLDVLEFDAQ
ASSISKGETLYDTVLTLDALGVDICVIRSGVEHYYEELVNSDNIHCAIVNGGDGSGQHPSQCLLDLMTIYEEFGKFEGLK
IAISGDLTHSRVAKSNMMMLQKLGARLYFTGPAAWYSEEFDDYGHYANLDRILPELDVHMLLRVQHERHDSGESFSKEGY
HTHFGLTQERAKMLKPTAIIMHPAPVNRDVEIADSLVESPQSRIVQQMSNGVYTRMAILEAILAGKKAK

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=267, Percent_Identity=33.3333333333333, Blast_Score=139, Evalue=3e-33,
Organism=Escherichia coli, GI2367364, Length=312, Percent_Identity=33.0128205128205, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI193204318, Length=283, Percent_Identity=35.3356890459364, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6322331, Length=272, Percent_Identity=31.9852941176471, Blast_Score=112, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24642586, Length=283, Percent_Identity=31.8021201413428, Blast_Score=131, Evalue=4e-31,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 34559; Mature: 34427

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKNGLVQLENLTSMENLSIDEVMGLIKRASAFKAGKADFALDEQTFASNLFFENSTRT
CCCCCCCEEHHHHCCHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHEECCCCCC
HHSFHIAERKLGLDVLEFDAQASSISKGETLYDTVLTLDALGVDICVIRSGVEHYYEELV
CHHHHHHHHHHCCHHHHHCCHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
NSDNIHCAIVNGGDGSGQHPSQCLLDLMTIYEEFGKFEGLKIAISGDLTHSRVAKSNMMM
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHH
LQKLGARLYFTGPAAWYSEEFDDYGHYANLDRILPELDVHMLLRVQHERHDSGESFSKEG
HHHHCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
YHTHFGLTQERAKMLKPTAIIMHPAPVNRDVEIADSLVESPQSRIVQQMSNGVYTRMAIL
CEECCCCCHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCHHHHHHHHHHCCHHHHHHHH
EAILAGKKAK
HHHHCCCCCC
>Mature Secondary Structure 
SVKNGLVQLENLTSMENLSIDEVMGLIKRASAFKAGKADFALDEQTFASNLFFENSTRT
CCCCCCEEHHHHCCHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHEECCCCCC
HHSFHIAERKLGLDVLEFDAQASSISKGETLYDTVLTLDALGVDICVIRSGVEHYYEELV
CHHHHHHHHHHCCHHHHHCCHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
NSDNIHCAIVNGGDGSGQHPSQCLLDLMTIYEEFGKFEGLKIAISGDLTHSRVAKSNMMM
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHH
LQKLGARLYFTGPAAWYSEEFDDYGHYANLDRILPELDVHMLLRVQHERHDSGESFSKEG
HHHHCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
YHTHFGLTQERAKMLKPTAIIMHPAPVNRDVEIADSLVESPQSRIVQQMSNGVYTRMAIL
CEECCCCCHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCHHHHHHHHHHCCHHHHHHHH
EAILAGKKAK
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]