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The map label for this gene is purB [H]

Identifier: 125623634

GI number: 125623634

Start: 771667

End: 772962

Strand: Direct

Name: purB [H]

Synonym: llmg_0783

Alternate gene names: 125623634

Gene position: 771667-772962 (Clockwise)

Preceding gene: 125623633

Following gene: 125623635

Centisome position: 30.51

GC content: 38.04

Gene sequence:

>1296_bases
GTGGTTATTAGTCGTTATTCACGTCCAGAAATGGCAGAAATTTGGTCAGAAGAAAACAAGTATAAAGCATGGCTTGAAGT
CGAAATTCTTGCAGATGAAGCTTGGGCAGAGCTTGGAGAAATCCCAGTAGCTGATGTGAAAAAAATTCGTGAAAAGGCAA
CTTTTAATGTTGAACGCATTCTTGAAATTGAAAAAGAAACTCGTCATGATGTGGTAGCTTTCACACGTTCTGTCAGTGAA
TCATTGGGTGAAGAACGTAAATGGGTCCATTACGGTCTGACATCAACAGATGTTGTGGATACAGCTTATGGCTATCTTTA
CAAACAAGCTAATGATATTTTACGCAAAGACTTGCAAAATTTCACAGAAATTGTGAAAACTAAAGCTTTGGAACATAAGT
ATACGGTCATGATGGGGCGTACACACGGGGTTCATGCAGAACCAACAACTTTTGGTTTGAAATTAGCCACTTGGTACAGC
GAAATGAAACGGAACATTGAACGCTTTGAACATGCGGCTAAAGCTGTTGAAGCTGGGAAAATTTCTGGTGCTGTGGGAAC
TTTTGCCAATATTCCTCCATTTGTTGAAGCTTATGTCTGTGAAAAATTAGGAACTCGTCCACAAGAAATTTCAACACAAG
TTTTGCCACGTGATTTGCACGCAGAATACTTCCAAACATTGGCGCTAATTGCGACTTCTATTGAACGCATGGCGACTGAA
ATCCGTGGATTACAAAAATCTGAACAACGTGAAGTTGAAGAATTCTTTGCTAAAGGTCAAAAAGGCTCGTCAGCAATGCC
ACACAAACGTAACCCAATTGGTTCTGAAAATATGACAGGACTTGCTCGTGTTATTCGTGGACATGCGATTACAGCTCTTG
AAGATGTTAGCCTTTGGCATGAGCGGGACATTTCTCACAGTTCTGCTGAACGGATTATTGCCCCAGATACAACAGAATTA
TTAGATTATATGCTCAATCGTTTTGGCAATATTGTTAAAAATTTGACGGTCTTTCCAGAAAATATGAAACGAAATATGGA
TGCGACTTTTGGTTTGATTTACAGTCAACATGTTATGTTAATGTTGATTGAAAAAGGAATGACTCGTGAAGCTGCTTATG
ATTTAGTTCAACCATTGACTGCAAAATCATGGGATGAACAAGTCATGTTTAGACCATTGCTTGAAGAAAATGAACAAATT
CGTCATTATTTGAATGATGAAGATATTGATAAAGCTTTTGATTATAACTATCATTTGACTCGTGTAGATGAAATTTTTAA
CCGTGTCTTTGATTGA

Upstream 100 bases:

>100_bases
ACGTTTCGTTCATTAGAAGATTAAAAATATAGGAATAATTTACTGACAGCTCTAAGGGCTAGCACCGAGGAAATTCTGTC
AGTAACAAAAGGAGTAAAAA

Downstream 100 bases:

>100_bases
TTTAAAAGGCCTACTGAAAAGTAGGCTTTTTTGTTATTAAAACGAAATATAAAAAGGATTGACAAAATAAATGAAAACGG
ATACAATCATACATGTAAAT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MVISRYSRPEMAEIWSEENKYKAWLEVEILADEAWAELGEIPVADVKKIREKATFNVERILEIEKETRHDVVAFTRSVSE
SLGEERKWVHYGLTSTDVVDTAYGYLYKQANDILRKDLQNFTEIVKTKALEHKYTVMMGRTHGVHAEPTTFGLKLATWYS
EMKRNIERFEHAAKAVEAGKISGAVGTFANIPPFVEAYVCEKLGTRPQEISTQVLPRDLHAEYFQTLALIATSIERMATE
IRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHAITALEDVSLWHERDISHSSAERIIAPDTTEL
LDYMLNRFGNIVKNLTVFPENMKRNMDATFGLIYSQHVMLMLIEKGMTREAAYDLVQPLTAKSWDEQVMFRPLLEENEQI
RHYLNDEDIDKAFDYNYHLTRVDEIFNRVFD

Sequences:

>Translated_431_residues
MVISRYSRPEMAEIWSEENKYKAWLEVEILADEAWAELGEIPVADVKKIREKATFNVERILEIEKETRHDVVAFTRSVSE
SLGEERKWVHYGLTSTDVVDTAYGYLYKQANDILRKDLQNFTEIVKTKALEHKYTVMMGRTHGVHAEPTTFGLKLATWYS
EMKRNIERFEHAAKAVEAGKISGAVGTFANIPPFVEAYVCEKLGTRPQEISTQVLPRDLHAEYFQTLALIATSIERMATE
IRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHAITALEDVSLWHERDISHSSAERIIAPDTTEL
LDYMLNRFGNIVKNLTVFPENMKRNMDATFGLIYSQHVMLMLIEKGMTREAAYDLVQPLTAKSWDEQVMFRPLLEENEQI
RHYLNDEDIDKAFDYNYHLTRVDEIFNRVFD
>Mature_431_residues
MVISRYSRPEMAEIWSEENKYKAWLEVEILADEAWAELGEIPVADVKKIREKATFNVERILEIEKETRHDVVAFTRSVSE
SLGEERKWVHYGLTSTDVVDTAYGYLYKQANDILRKDLQNFTEIVKTKALEHKYTVMMGRTHGVHAEPTTFGLKLATWYS
EMKRNIERFEHAAKAVEAGKISGAVGTFANIPPFVEAYVCEKLGTRPQEISTQVLPRDLHAEYFQTLALIATSIERMATE
IRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHAITALEDVSLWHERDISHSSAERIIAPDTTEL
LDYMLNRFGNIVKNLTVFPENMKRNMDATFGLIYSQHVMLMLIEKGMTREAAYDLVQPLTAKSWDEQVMFRPLLEENEQI
RHYLNDEDIDKAFDYNYHLTRVDEIFNRVFD

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=397, Percent_Identity=27.7078085642317, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI183227688, Length=399, Percent_Identity=28.3208020050125, Blast_Score=149, Evalue=6e-36,
Organism=Escherichia coli, GI1787376, Length=285, Percent_Identity=30.1754385964912, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17508577, Length=396, Percent_Identity=27.7777777777778, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI32564234, Length=335, Percent_Identity=28.6567164179104, Blast_Score=101, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6323391, Length=397, Percent_Identity=27.9596977329975, Blast_Score=147, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24647570, Length=423, Percent_Identity=24.113475177305, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49736; Mature: 49736

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVISRYSRPEMAEIWSEENKYKAWLEVEILADEAWAELGEIPVADVKKIREKATFNVERI
CCCCCCCCCHHHHHHCCCCCEEEEEEEEEECHHHHHHHCCCCHHHHHHHHHHHHCCHHHH
LEIEKETRHDVVAFTRSVSESLGEERKWVHYGLTSTDVVDTAYGYLYKQANDILRKDLQN
HHHHHHHHHHHHHHHHHHHHHHCHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FTEIVKTKALEHKYTVMMGRTHGVHAEPTTFGLKLATWYSEMKRNIERFEHAAKAVEAGK
HHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ISGAVGTFANIPPFVEAYVCEKLGTRPQEISTQVLPRDLHAEYFQTLALIATSIERMATE
CCCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
IRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHAITALEDVSLWH
HHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHH
ERDISHSSAERIIAPDTTELLDYMLNRFGNIVKNLTVFPENMKRNMDATFGLIYSQHVML
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHH
MLIEKGMTREAAYDLVQPLTAKSWDEQVMFRPLLEENEQIRHYLNDEDIDKAFDYNYHLT
HHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHCCCCHHH
RVDEIFNRVFD
HHHHHHHHHCC
>Mature Secondary Structure
MVISRYSRPEMAEIWSEENKYKAWLEVEILADEAWAELGEIPVADVKKIREKATFNVERI
CCCCCCCCCHHHHHHCCCCCEEEEEEEEEECHHHHHHHCCCCHHHHHHHHHHHHCCHHHH
LEIEKETRHDVVAFTRSVSESLGEERKWVHYGLTSTDVVDTAYGYLYKQANDILRKDLQN
HHHHHHHHHHHHHHHHHHHHHHCHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FTEIVKTKALEHKYTVMMGRTHGVHAEPTTFGLKLATWYSEMKRNIERFEHAAKAVEAGK
HHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ISGAVGTFANIPPFVEAYVCEKLGTRPQEISTQVLPRDLHAEYFQTLALIATSIERMATE
CCCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
IRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHAITALEDVSLWH
HHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHH
ERDISHSSAERIIAPDTTELLDYMLNRFGNIVKNLTVFPENMKRNMDATFGLIYSQHVML
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHH
MLIEKGMTREAAYDLVQPLTAKSWDEQVMFRPLLEENEQIRHYLNDEDIDKAFDYNYHLT
HHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHCCCCHHH
RVDEIFNRVFD
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12397186 [H]