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The map label for this gene is amyY [C]

Identifier: 125623597

GI number: 125623597

Start: 726850

End: 728424

Strand: Direct

Name: amyY [C]

Synonym: llmg_0743

Alternate gene names: 125623597

Gene position: 726850-728424 (Clockwise)

Preceding gene: 125623596

Following gene: 125623598

Centisome position: 28.74

GC content: 39.68

Gene sequence:

>1575_bases
ATGAAAAATAAGCTTTTACTTCTGTCAGTAACTTTACTGGCGACCTTTGCTTTAAGTGCTTGTCAAAAAGTAACTTCAAA
AGATTCATCAGTAAAAAAAGCGGCTGTCAGTCAAAAGGTTGATACGAGTTTGTATCGTAATTTTTATGAAATATTTACGA
GTTCATTTGCGGATTCAAATCATGATGGTGAGGGCGATTTAAATGGGGTCACGCAACATTTAGATTATCTCAATACGGGG
AATAAAAATTCTACAACAGATTTGAAAGTCCAAGGACTTTGGATGACCCCAATCTTTGCTAGTCCAAGCTATCATGGCTA
TGATGTCAGCAATTACGAAGAAATTAATCCTAAATTTGGCACAATGGCTGATTTTGAAAATCTGATTGCTCAGGCTAAAA
AACGAGGAATTGCCGTGATTTTGGACATGCCGTTTAACCACACGGCGACTGACAATGTCTGGTTTCAAAAAGCACTAGCT
GGCGACAAGAAATATATGGCTTATTATAATTGGTCGGACACAGCAAAAGAGGGTTATAGCTTAGCCAGCAACGGCAAATA
CTATGAATCAGAGTTTGATAAATCGATGCCAGACCTCAATCTTGCCAATCCTGAGGTCAAAAAAGAAATTGTAAAAATTA
CGAAATTTTGGTTAGACAAAGGTGTTTCTGGTTTTCGTTTGGACGCTGTCGGTTTTTATTTCTCAAATGATGATAAAAAA
ACGACGGCCTTTACCAAATGGCTGACAGATACGGTCAAAAAGCAAAATCCAAAAGCTTATTTAGTGGGTGAAGTTTTTTC
AACGGCTTCGGCAATTGATGATATTTATCAGTCAGGTGTTGATTCTTTATTTGATTTTCCAAATGCTTTGCAATCGAGTT
CGAGTCCGATTAATTCGGCTATGGTCTTGGGCGAAGGCTCATTGTTTGGTCAGCAAATCGAAGCTTGGGACCAAGAAATT
CATGCTGACAACCCGAAAGCTATTGACGCTCCTTTTTTGAGTAATCATGACACAGACCGGTCAGCTACGCTTTTAAGCTC
TTTAGCCATGCAAAAAATGGCTGCTCAAACTTACCTTTTGATGCCGGGAAATCCATTTATTTACTACGGCGAAGAATTGG
GGCTGACAGGTTCTGGTATTGACCAAAACAAACGCTTGCCAATGCCATGGTCGGCTGATGGAGCAGATTCGCCTAAGGCA
AATGTTGCTGTTCCCGGTGCGACTGAAACGGCGACAACTGACGGAGGATCGGTGGCTCAGCAAGAAACGAATCCGAATTC
GCTACTCAATTGGTACAAAAAAATCTTGCGGGTCAAAGCGAAATATCCTGAAATTGCGACTGCCAGGCTGAAAAATATTT
CTGTCAGTAAAGATAGCTTGTCTGTCATTAATTATGGAAAGGATTTGACCATTGTCAATAATTTTTCAGATAAAGAAAGT
ATGACAGTGAAACTCCCGAAAGGAGTTGTCGGCAAAAAAATTACTGACCAACTTTCTGGTGGTGGAGTCCAAGCGACATT
GACTGATGGAAAATTGACAATTCCAGCTTACAGTACAGTTATTTTGAAAAAATAA

Upstream 100 bases:

>100_bases
TTGCCAGCAAATGTGATTGCAGTAGGTAATCCGGCACGAATTCTCCGCGAAATTACTGATTCTGACCGACTTTACTGGGA
AGAAAAAGAGAACGAATATT

Downstream 100 bases:

>100_bases
ATAAGTTCAGTTTAGGGACTTTAAAAATGAAAATGTAAAACTATGAAAAAAACAAAAACAAATGATTTTCACAAGATGGT
CGGTTATCAGATTTATCCAA

Product: alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MKNKLLLLSVTLLATFALSACQKVTSKDSSVKKAAVSQKVDTSLYRNFYEIFTSSFADSNHDGEGDLNGVTQHLDYLNTG
NKNSTTDLKVQGLWMTPIFASPSYHGYDVSNYEEINPKFGTMADFENLIAQAKKRGIAVILDMPFNHTATDNVWFQKALA
GDKKYMAYYNWSDTAKEGYSLASNGKYYESEFDKSMPDLNLANPEVKKEIVKITKFWLDKGVSGFRLDAVGFYFSNDDKK
TTAFTKWLTDTVKKQNPKAYLVGEVFSTASAIDDIYQSGVDSLFDFPNALQSSSSPINSAMVLGEGSLFGQQIEAWDQEI
HADNPKAIDAPFLSNHDTDRSATLLSSLAMQKMAAQTYLLMPGNPFIYYGEELGLTGSGIDQNKRLPMPWSADGADSPKA
NVAVPGATETATTDGGSVAQQETNPNSLLNWYKKILRVKAKYPEIATARLKNISVSKDSLSVINYGKDLTIVNNFSDKES
MTVKLPKGVVGKKITDQLSGGGVQATLTDGKLTIPAYSTVILKK

Sequences:

>Translated_524_residues
MKNKLLLLSVTLLATFALSACQKVTSKDSSVKKAAVSQKVDTSLYRNFYEIFTSSFADSNHDGEGDLNGVTQHLDYLNTG
NKNSTTDLKVQGLWMTPIFASPSYHGYDVSNYEEINPKFGTMADFENLIAQAKKRGIAVILDMPFNHTATDNVWFQKALA
GDKKYMAYYNWSDTAKEGYSLASNGKYYESEFDKSMPDLNLANPEVKKEIVKITKFWLDKGVSGFRLDAVGFYFSNDDKK
TTAFTKWLTDTVKKQNPKAYLVGEVFSTASAIDDIYQSGVDSLFDFPNALQSSSSPINSAMVLGEGSLFGQQIEAWDQEI
HADNPKAIDAPFLSNHDTDRSATLLSSLAMQKMAAQTYLLMPGNPFIYYGEELGLTGSGIDQNKRLPMPWSADGADSPKA
NVAVPGATETATTDGGSVAQQETNPNSLLNWYKKILRVKAKYPEIATARLKNISVSKDSLSVINYGKDLTIVNNFSDKES
MTVKLPKGVVGKKITDQLSGGGVQATLTDGKLTIPAYSTVILKK
>Mature_524_residues
MKNKLLLLSVTLLATFALSACQKVTSKDSSVKKAAVSQKVDTSLYRNFYEIFTSSFADSNHDGEGDLNGVTQHLDYLNTG
NKNSTTDLKVQGLWMTPIFASPSYHGYDVSNYEEINPKFGTMADFENLIAQAKKRGIAVILDMPFNHTATDNVWFQKALA
GDKKYMAYYNWSDTAKEGYSLASNGKYYESEFDKSMPDLNLANPEVKKEIVKITKFWLDKGVSGFRLDAVGFYFSNDDKK
TTAFTKWLTDTVKKQNPKAYLVGEVFSTASAIDDIYQSGVDSLFDFPNALQSSSSPINSAMVLGEGSLFGQQIEAWDQEI
HADNPKAIDAPFLSNHDTDRSATLLSSLAMQKMAAQTYLLMPGNPFIYYGEELGLTGSGIDQNKRLPMPWSADGADSPKA
NVAVPGATETATTDGGSVAQQETNPNSLLNWYKKILRVKAKYPEIATARLKNISVSKDSLSVINYGKDLTIVNNFSDKES
MTVKLPKGVVGKKITDQLSGGGVQATLTDGKLTIPAYSTVILKK

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=495, Percent_Identity=28.4848484848485, Blast_Score=156, Evalue=4e-38,
Organism=Escherichia coli, GI1790687, Length=371, Percent_Identity=33.6927223719677, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1786604, Length=518, Percent_Identity=24.7104247104247, Blast_Score=122, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI32565753, Length=498, Percent_Identity=23.4939759036145, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25147709, Length=424, Percent_Identity=21.2264150943396, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322245, Length=547, Percent_Identity=24.8628884826325, Blast_Score=128, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6321731, Length=208, Percent_Identity=32.6923076923077, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319776, Length=208, Percent_Identity=32.6923076923077, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321726, Length=502, Percent_Identity=22.7091633466135, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6322241, Length=502, Percent_Identity=23.1075697211155, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6322021, Length=502, Percent_Identity=23.1075697211155, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6324416, Length=501, Percent_Identity=22.1556886227545, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI45549022, Length=549, Percent_Identity=30.7832422586521, Blast_Score=178, Evalue=9e-45,
Organism=Drosophila melanogaster, GI221330053, Length=620, Percent_Identity=25.6451612903226, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24586593, Length=527, Percent_Identity=27.5142314990512, Blast_Score=162, Evalue=8e-40,
Organism=Drosophila melanogaster, GI24586587, Length=413, Percent_Identity=28.8135593220339, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24583745, Length=474, Percent_Identity=28.4810126582279, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24583747, Length=387, Percent_Identity=28.9405684754522, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24583749, Length=388, Percent_Identity=28.8659793814433, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24586591, Length=203, Percent_Identity=37.4384236453202, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24586599, Length=526, Percent_Identity=26.2357414448669, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24586597, Length=389, Percent_Identity=30.3341902313625, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24586589, Length=518, Percent_Identity=27.2200772200772, Blast_Score=145, Evalue=1e-34,
Organism=Drosophila melanogaster, GI281360393, Length=468, Percent_Identity=25.6410256410256, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 57487; Mature: 57487

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKLLLLSVTLLATFALSACQKVTSKDSSVKKAAVSQKVDTSLYRNFYEIFTSSFADSN
CCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HDGEGDLNGVTQHLDYLNTGNKNSTTDLKVQGLWMTPIFASPSYHGYDVSNYEEINPKFG
CCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCHHCCCCCC
TMADFENLIAQAKKRGIAVILDMPFNHTATDNVWFQKALAGDKKYMAYYNWSDTAKEGYS
CHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHCCCCEEEEEECCCCHHHHCCC
LASNGKYYESEFDKSMPDLNLANPEVKKEIVKITKFWLDKGVSGFRLDAVGFYFSNDDKK
CCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCC
TTAFTKWLTDTVKKQNPKAYLVGEVFSTASAIDDIYQSGVDSLFDFPNALQSSSSPINSA
HHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCE
MVLGEGSLFGQQIEAWDQEIHADNPKAIDAPFLSNHDTDRSATLLSSLAMQKMAAQTYLL
EEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEE
MPGNPFIYYGEELGLTGSGIDQNKRLPMPWSADGADSPKANVAVPGATETATTDGGSVAQ
ECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCC
QETNPNSLLNWYKKILRVKAKYPEIATARLKNISVSKDSLSVINYGKDLTIVNNFSDKES
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCEEECCCCEEEEECCCCCCC
MTVKLPKGVVGKKITDQLSGGGVQATLTDGKLTIPAYSTVILKK
EEEECCCCCCCCHHHHHHCCCCEEEEEECCEEECCCEEEEEEEC
>Mature Secondary Structure
MKNKLLLLSVTLLATFALSACQKVTSKDSSVKKAAVSQKVDTSLYRNFYEIFTSSFADSN
CCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HDGEGDLNGVTQHLDYLNTGNKNSTTDLKVQGLWMTPIFASPSYHGYDVSNYEEINPKFG
CCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCHHCCCCCC
TMADFENLIAQAKKRGIAVILDMPFNHTATDNVWFQKALAGDKKYMAYYNWSDTAKEGYS
CHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHCCCCEEEEEECCCCHHHHCCC
LASNGKYYESEFDKSMPDLNLANPEVKKEIVKITKFWLDKGVSGFRLDAVGFYFSNDDKK
CCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCC
TTAFTKWLTDTVKKQNPKAYLVGEVFSTASAIDDIYQSGVDSLFDFPNALQSSSSPINSA
HHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCE
MVLGEGSLFGQQIEAWDQEIHADNPKAIDAPFLSNHDTDRSATLLSSLAMQKMAAQTYLL
EEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEE
MPGNPFIYYGEELGLTGSGIDQNKRLPMPWSADGADSPKANVAVPGATETATTDGGSVAQ
ECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCC
QETNPNSLLNWYKKILRVKAKYPEIATARLKNISVSKDSLSVINYGKDLTIVNNFSDKES
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCEEECCCCEEEEECCCCCCC
MTVKLPKGVVGKKITDQLSGGGVQATLTDGKLTIPAYSTVILKK
EEEECCCCCCCCHHHHHHCCCCEEEEEECCEEECCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2455281 [H]