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The map label for this gene is hsdM [H]

Identifier: 125623518

GI number: 125623518

Start: 654041

End: 655678

Strand: Direct

Name: hsdM [H]

Synonym: llmg_0659

Alternate gene names: 125623518

Gene position: 654041-655678 (Clockwise)

Preceding gene: 125623517

Following gene: 125623519

Centisome position: 25.86

GC content: 39.87

Gene sequence:

>1638_bases
ATGGAAAGAAGAATTATAATGGCGACAGGTTTAAATCAACAACTATGGGCTTCTGCGGATATCTTACGTGGGAAGATGGA
TGCAAGTGAGTACAAAAACTACTTACTGGGATTAATTTTCTATAAGTACTTGTCTGATGCGCAGCTTAGAGAAGTTTATG
AACAAGAAAATGGAAAAACAGATACCTTCCCAGAACGTTCCACTCAGTATGCAGGATTCATGGAGTGGTACGAAGAAGAC
AAAGACGATTTAATCGAAAATATCCAACCCAAACAAGGTTACTTTATCCAACCCGATCAATTGTTTTATCACTACCGCAT
CAAAGCAGACAACTATGAATTTAATTTGACCGACTTACAAGCAGGTTTTAATGAGTTGGAACGTCAAGGAGAAGAATTCA
GTGGATTGTTTGCGGATATTGATTTAAACTCCACAAAATTAGGCTCAAATGCACAACAACGTAATGTGACGATTACTGAG
GTCTTGCGTGCCTTAGATGAGATTGACTTATTTGAACACAATGGCGATGTGATTGGGGACGCTTATGAGTATTTAATCGG
GATGTTTGCGGCAGGTGCAGGTAAAAAAGCAGGAGAGTTTTATACCCCTCAAGCTGTTTCACGCATCATGTCAGAAATTA
CGTCGATCGGACAAGAATCTCGAGCGCCTTTTCATATTTACGATCCTGCGATGGGTTCGGGTTCTTTGATGCTTAATATC
CGTCGCTATCTCATCAATCCAAATCAAGTGCACTATCATGGACAAGAGTTAAATACGACGACCTTTAACTTAGCACGTAT
GAACTTAATTCTTCATGGAGTAGATAAAGAACGCATGAACCTGAATAACGGAGATACTTTAGACGCTGATTGGCCCTCAG
AAGAGCCTTATCAGTTTGATTCTGTGGTCATGAACCCTCCTTACTCTGCGAAATGGTCAGCGGCAGATAAGTTTCTCTCT
GATCCTCGCTTTGAACGTTTTGGAAAATTAGCGCCTAAATCTAAAGCGGACTTTGCCTTTCTCCTTCACGGTTTTTACCA
TTTGAAAGAATCAGGAACAATGGGGATTGTCCTGCCTCATGGCGTGCTCTTTAGAGGAGCCGCAGAAGGAACCATCCGTC
AAGCCCTTTTAGAGATGGGAGCCATTGATGCGGTCATTGGCTTGCCGGCCAATATCTTTTTTGGAACGAGTATTCCGACA
ACGGTTATTATTTTGAAGAAAAACCGCTCTCGTCGTGATGTCTTGTTTATTGATGCTTCTCAAGACTTTGAAAAACAAAA
AAATCAAAATGTCCTGTTGGATGAACATATTGATAAAATTGTTTCTACCTACAAAAAGCGAGAAGATATTGAAAGATATG
CTCATGTTGCAAGTTTTGATGAGATCCAAGAAAATGACTTTAACTTAAATATCCCTCGTTATGTCGATACCTTTGAGGAA
GAAGAACCGGTTGATTTGGTTGCAGTGAATACCAATCTCCTTAAGATCAATGAAGAATTAGTTCAACAAGAGCAAGTGCT
CTTATCGTTGATTAACGATTTTTCAGAAAGTGAAGAGAATCAAGCCTTGATTGAATCGATGCGTCTTCTTTTGAGAGGCG
GTCATGATGAGTATAAAAAGTCCACAATTAAGGTTTGA

Upstream 100 bases:

>100_bases
TTAGTAAAGAAGAGTTCGAAGCCAAAACGGGTGAAAAATACAGAAGAAGAACAAAAGTTTTAGAAGAACGACTACGACAA
AACTTTGAACAAATTCAAAA

Downstream 100 bases:

>100_bases
AGGTTTTACGGATGATTGGGAACAGCGTAAGTTAGGGGAACTTTCTCAAAAAATATCAGTAGGCATTGCTACATCTTCGA
GTAAATATTTTAGTTCTCAA

Product: putative type I site-specific deoxyribonuclease

Products: NA

Alternate protein names: M.EcoR124II [H]

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MERRIIMATGLNQQLWASADILRGKMDASEYKNYLLGLIFYKYLSDAQLREVYEQENGKTDTFPERSTQYAGFMEWYEED
KDDLIENIQPKQGYFIQPDQLFYHYRIKADNYEFNLTDLQAGFNELERQGEEFSGLFADIDLNSTKLGSNAQQRNVTITE
VLRALDEIDLFEHNGDVIGDAYEYLIGMFAAGAGKKAGEFYTPQAVSRIMSEITSIGQESRAPFHIYDPAMGSGSLMLNI
RRYLINPNQVHYHGQELNTTTFNLARMNLILHGVDKERMNLNNGDTLDADWPSEEPYQFDSVVMNPPYSAKWSAADKFLS
DPRFERFGKLAPKSKADFAFLLHGFYHLKESGTMGIVLPHGVLFRGAAEGTIRQALLEMGAIDAVIGLPANIFFGTSIPT
TVIILKKNRSRRDVLFIDASQDFEKQKNQNVLLDEHIDKIVSTYKKREDIERYAHVASFDEIQENDFNLNIPRYVDTFEE
EEPVDLVAVNTNLLKINEELVQQEQVLLSLINDFSESEENQALIESMRLLLRGGHDEYKKSTIKV

Sequences:

>Translated_545_residues
MERRIIMATGLNQQLWASADILRGKMDASEYKNYLLGLIFYKYLSDAQLREVYEQENGKTDTFPERSTQYAGFMEWYEED
KDDLIENIQPKQGYFIQPDQLFYHYRIKADNYEFNLTDLQAGFNELERQGEEFSGLFADIDLNSTKLGSNAQQRNVTITE
VLRALDEIDLFEHNGDVIGDAYEYLIGMFAAGAGKKAGEFYTPQAVSRIMSEITSIGQESRAPFHIYDPAMGSGSLMLNI
RRYLINPNQVHYHGQELNTTTFNLARMNLILHGVDKERMNLNNGDTLDADWPSEEPYQFDSVVMNPPYSAKWSAADKFLS
DPRFERFGKLAPKSKADFAFLLHGFYHLKESGTMGIVLPHGVLFRGAAEGTIRQALLEMGAIDAVIGLPANIFFGTSIPT
TVIILKKNRSRRDVLFIDASQDFEKQKNQNVLLDEHIDKIVSTYKKREDIERYAHVASFDEIQENDFNLNIPRYVDTFEE
EEPVDLVAVNTNLLKINEELVQQEQVLLSLINDFSESEENQALIESMRLLLRGGHDEYKKSTIKV
>Mature_545_residues
MERRIIMATGLNQQLWASADILRGKMDASEYKNYLLGLIFYKYLSDAQLREVYEQENGKTDTFPERSTQYAGFMEWYEED
KDDLIENIQPKQGYFIQPDQLFYHYRIKADNYEFNLTDLQAGFNELERQGEEFSGLFADIDLNSTKLGSNAQQRNVTITE
VLRALDEIDLFEHNGDVIGDAYEYLIGMFAAGAGKKAGEFYTPQAVSRIMSEITSIGQESRAPFHIYDPAMGSGSLMLNI
RRYLINPNQVHYHGQELNTTTFNLARMNLILHGVDKERMNLNNGDTLDADWPSEEPYQFDSVVMNPPYSAKWSAADKFLS
DPRFERFGKLAPKSKADFAFLLHGFYHLKESGTMGIVLPHGVLFRGAAEGTIRQALLEMGAIDAVIGLPANIFFGTSIPT
TVIILKKNRSRRDVLFIDASQDFEKQKNQNVLLDEHIDKIVSTYKKREDIERYAHVASFDEIQENDFNLNIPRYVDTFEE
EEPVDLVAVNTNLLKINEELVQQEQVLLSLINDFSESEENQALIESMRLLLRGGHDEYKKSTIKV

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=323, Percent_Identity=25.3869969040248, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 62409; Mature: 62409

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERRIIMATGLNQQLWASADILRGKMDASEYKNYLLGLIFYKYLSDAQLREVYEQENGKT
CCCEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
DTFPERSTQYAGFMEWYEEDKDDLIENIQPKQGYFIQPDQLFYHYRIKADNYEFNLTDLQ
CCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCEEECCCCEEEEEEEEECCEEEEHHHHH
AGFNELERQGEEFSGLFADIDLNSTKLGSNAQQRNVTITEVLRALDEIDLFEHNGDVIGD
HHHHHHHHCCCHHCCEEEEECCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCHH
AYEYLIGMFAAGAGKKAGEFYTPQAVSRIMSEITSIGQESRAPFHIYDPAMGSGSLMLNI
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHCCCCEEEECCCCCCCCEEEEE
RRYLINPNQVHYHGQELNTTTFNLARMNLILHGVDKERMNLNNGDTLDADWPSEEPYQFD
HHHHCCCCEEEECCCCCCCCEEHHHEEEHEEECCCHHHCCCCCCCCCCCCCCCCCCCCCC
SVVMNPPYSAKWSAADKFLSDPRFERFGKLAPKSKADFAFLLHGFYHLKESGTMGIVLPH
CEEECCCCCCCCHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECC
GVLFRGAAEGTIRQALLEMGAIDAVIGLPANIFFGTSIPTTVIILKKNRSRRDVLFIDAS
CEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEECCCCCCCEEEEECC
QDFEKQKNQNVLLDEHIDKIVSTYKKREDIERYAHVASFDEIQENDFNLNIPRYVDTFEE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCC
EEPVDLVAVNTNLLKINEELVQQEQVLLSLINDFSESEENQALIESMRLLLRGGHDEYKK
CCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHH
STIKV
HCCCC
>Mature Secondary Structure
MERRIIMATGLNQQLWASADILRGKMDASEYKNYLLGLIFYKYLSDAQLREVYEQENGKT
CCCEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
DTFPERSTQYAGFMEWYEEDKDDLIENIQPKQGYFIQPDQLFYHYRIKADNYEFNLTDLQ
CCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCEEECCCCEEEEEEEEECCEEEEHHHHH
AGFNELERQGEEFSGLFADIDLNSTKLGSNAQQRNVTITEVLRALDEIDLFEHNGDVIGD
HHHHHHHHCCCHHCCEEEEECCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCHH
AYEYLIGMFAAGAGKKAGEFYTPQAVSRIMSEITSIGQESRAPFHIYDPAMGSGSLMLNI
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHCCCCEEEECCCCCCCCEEEEE
RRYLINPNQVHYHGQELNTTTFNLARMNLILHGVDKERMNLNNGDTLDADWPSEEPYQFD
HHHHCCCCEEEECCCCCCCCEEHHHEEEHEEECCCHHHCCCCCCCCCCCCCCCCCCCCCC
SVVMNPPYSAKWSAADKFLSDPRFERFGKLAPKSKADFAFLLHGFYHLKESGTMGIVLPH
CEEECCCCCCCCHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECC
GVLFRGAAEGTIRQALLEMGAIDAVIGLPANIFFGTSIPTTVIILKKNRSRRDVLFIDAS
CEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEECCCCCCCEEEEECC
QDFEKQKNQNVLLDEHIDKIVSTYKKREDIERYAHVASFDEIQENDFNLNIPRYVDTFEE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCC
EEPVDLVAVNTNLLKINEELVQQEQVLLSLINDFSESEENQALIESMRLLLRGGHDEYKK
CCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHH
STIKV
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]