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The map label for this gene is fadD [H]

Identifier: 125623487

GI number: 125623487

Start: 615570

End: 617093

Strand: Reverse

Name: fadD [H]

Synonym: llmg_0627

Alternate gene names: 125623487

Gene position: 617093-615570 (Counterclockwise)

Preceding gene: 125623498

Following gene: 125623486

Centisome position: 24.4

GC content: 38.45

Gene sequence:

>1524_bases
TTGAAATTCAAACATGAACCCCTAAACCTATATCTAAACTTTAAAAAATCAGCAGAACAATTTCCTTATAGCCCAATTAT
TTTTGACCAAACTGTTGGTGCCTTCCCTGAATTAGCTCTGGAAACAACTTACCAAAAGACCCATCAAGCAATTGTTGATA
AGGCAAGCCTTTTATCGGAACTAGGAATCAAAAAATCTGACAAAGTCATTATTTTTAAATCAAGTGCTTTTGATACCTAT
CTTCTTGCCGTAGCTGTTTCTTATCTCGGCGCTGTTCCAGCAATGATTTCTTACCACCTTCCAGCTGAAACAATGGATAT
TTTAGCTGGTCGTTTAGATGACCCTTGGCTCATTTATGACCAAGTGACTGACCAGACTGTGACAGAGATGAAAAATCTTA
AGAATTCTAAAAAACTTTCTGTTAGCAAATTTGCTTCGCAAAAACCAAGTCATCTTGTTGACAGTCAGCAACTGGATAAT
GACGAGATTGCTTATCTCACTCATACTTCTGGAACAACTGGTGTTCCGAAATTGATTGCCCACTCTGCCAATTCTATGGG
CTGGCGTTGGGTGCTTCAAAGAACGGTCATGGATTGGATGCCCGATAAAAAAGAAATTTTGGCTTTCCACATTTCGCCCG
TTCATTCTCGTTTCAATATTGGCGTTTCATCTGCCATGACCTTTGGTTTTGGCCTAATGCCACTTAATGATTTATCAAGA
ATTAATCTTGTCAATCTATTTTCTAAACATCAGCCTTATGCTTTTGAAACTCACCCTAATGACTTTGTTCGCTTGGCGAA
TCTTGCGAAGCAGGAACCTGAAATTTTTTCTTCAATTCGCTATCTTCATTCAACTTTTGATGCGATTAATAAAGAAACAA
TGCATACTTTCTTATCAGCCTCTAAACACGACAACCCAGTCTTTTTACAAATCTATGGTCAGTCAGAATGCGGTCCAATG
ATTTGGAAAAAACACCGTTTATCAACCCTAGCAGATACTGATGCTCGTGAAATGGGAATTGGTATGCCTGGTCTTTCTAA
GGCACGTATTGCTGACGAAAATGGAAATGAATGCCCTGCAGGTATTCCTGGAAGAATTCATTTCCTTTCTAAAGGACGTG
CTTTGACTTACTACAAAGAAGAGGATCGTTTCAACAAAGAAGTCTATGGCGACTGGTGGGATACTGGCGATTGGGGCCTA
ATGAATGAAGAAGGAAATCTTTTCCTCCATGACCGTCAAGTCGATTTAATTGATAAAATTGAATCAAATTTAGCAATTGA
AGACCTCCTTTTAGATCAACATGATTTTTTAGATGAAGTTGTGATTATTCGCGATAAATCTGGAAAACCACAACCTATCT
TAGCCTTGGCTGACAATGCGGAAATGGATTGGTCAGCTTGGTGGGCTTCAATTGTTGACTTACCATTTTTAAATGAACCT
ATTCTCATGGCTTACGATGAAATTCCACGTACTGCAACAATGAAAGTTCAAAGATTAGCTCTTGAAAGAGACTTAGCCAA
ATAA

Upstream 100 bases:

>100_bases
ATTATTTTATTTCAAAGCCTTAACGAAAAGTTTTTTGAAGGAAGTTGGTCTAAATAATACTTTGTGATATAATAAACTTA
TTAACTTGAGGAGACTTTTT

Downstream 100 bases:

>100_bases
ATTATCCTAAATATTTTGCTGATTAATATCTGTCAGTAAATTAATTTATCAAAAAACTTAAAAGTAAAACTACTGACAGG
TCTGTCAGTAGTTTTTTTCA

Product: long-chain acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MKFKHEPLNLYLNFKKSAEQFPYSPIIFDQTVGAFPELALETTYQKTHQAIVDKASLLSELGIKKSDKVIIFKSSAFDTY
LLAVAVSYLGAVPAMISYHLPAETMDILAGRLDDPWLIYDQVTDQTVTEMKNLKNSKKLSVSKFASQKPSHLVDSQQLDN
DEIAYLTHTSGTTGVPKLIAHSANSMGWRWVLQRTVMDWMPDKKEILAFHISPVHSRFNIGVSSAMTFGFGLMPLNDLSR
INLVNLFSKHQPYAFETHPNDFVRLANLAKQEPEIFSSIRYLHSTFDAINKETMHTFLSASKHDNPVFLQIYGQSECGPM
IWKKHRLSTLADTDAREMGIGMPGLSKARIADENGNECPAGIPGRIHFLSKGRALTYYKEEDRFNKEVYGDWWDTGDWGL
MNEEGNLFLHDRQVDLIDKIESNLAIEDLLLDQHDFLDEVVIIRDKSGKPQPILALADNAEMDWSAWWASIVDLPFLNEP
ILMAYDEIPRTATMKVQRLALERDLAK

Sequences:

>Translated_507_residues
MKFKHEPLNLYLNFKKSAEQFPYSPIIFDQTVGAFPELALETTYQKTHQAIVDKASLLSELGIKKSDKVIIFKSSAFDTY
LLAVAVSYLGAVPAMISYHLPAETMDILAGRLDDPWLIYDQVTDQTVTEMKNLKNSKKLSVSKFASQKPSHLVDSQQLDN
DEIAYLTHTSGTTGVPKLIAHSANSMGWRWVLQRTVMDWMPDKKEILAFHISPVHSRFNIGVSSAMTFGFGLMPLNDLSR
INLVNLFSKHQPYAFETHPNDFVRLANLAKQEPEIFSSIRYLHSTFDAINKETMHTFLSASKHDNPVFLQIYGQSECGPM
IWKKHRLSTLADTDAREMGIGMPGLSKARIADENGNECPAGIPGRIHFLSKGRALTYYKEEDRFNKEVYGDWWDTGDWGL
MNEEGNLFLHDRQVDLIDKIESNLAIEDLLLDQHDFLDEVVIIRDKSGKPQPILALADNAEMDWSAWWASIVDLPFLNEP
ILMAYDEIPRTATMKVQRLALERDLAK
>Mature_507_residues
MKFKHEPLNLYLNFKKSAEQFPYSPIIFDQTVGAFPELALETTYQKTHQAIVDKASLLSELGIKKSDKVIIFKSSAFDTY
LLAVAVSYLGAVPAMISYHLPAETMDILAGRLDDPWLIYDQVTDQTVTEMKNLKNSKKLSVSKFASQKPSHLVDSQQLDN
DEIAYLTHTSGTTGVPKLIAHSANSMGWRWVLQRTVMDWMPDKKEILAFHISPVHSRFNIGVSSAMTFGFGLMPLNDLSR
INLVNLFSKHQPYAFETHPNDFVRLANLAKQEPEIFSSIRYLHSTFDAINKETMHTFLSASKHDNPVFLQIYGQSECGPM
IWKKHRLSTLADTDAREMGIGMPGLSKARIADENGNECPAGIPGRIHFLSKGRALTYYKEEDRFNKEVYGDWWDTGDWGL
MNEEGNLFLHDRQVDLIDKIESNLAIEDLLLDQHDFLDEVVIIRDKSGKPQPILALADNAEMDWSAWWASIVDLPFLNEP
ILMAYDEIPRTATMKVQRLALERDLAK

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788107, Length=546, Percent_Identity=23.992673992674, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=385, Percent_Identity=23.6363636363636, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 57574; Mature: 57574

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKHEPLNLYLNFKKSAEQFPYSPIIFDQTVGAFPELALETTYQKTHQAIVDKASLLSE
CCCCCCCEEEEEEECHHHHHCCCCCEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGIKKSDKVIIFKSSAFDTYLLAVAVSYLGAVPAMISYHLPAETMDILAGRLDDPWLIYD
HCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEE
QVTDQTVTEMKNLKNSKKLSVSKFASQKPSHLVDSQQLDNDEIAYLTHTSGTTGVPKLIA
CCCHHHHHHHHHCCCCCCCHHHHHHCCCCCHHCCHHCCCCCCEEEEEECCCCCCHHHHHH
HSANSMGWRWVLQRTVMDWMPDKKEILAFHISPVHSRFNIGVSSAMTFGFGLMPLNDLSR
HCCCCCHHHHHHHHHHHHHCCCHHHEEEEEECCCCHHHCCCHHHHHHHCCCCCCCCCCCH
INLVNLFSKHQPYAFETHPNDFVRLANLAKQEPEIFSSIRYLHSTFDAINKETMHTFLSA
HHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKHDNPVFLQIYGQSECGPMIWKKHRLSTLADTDAREMGIGMPGLSKARIADENGNECPA
CCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEECCCCCCCCC
GIPGRIHFLSKGRALTYYKEEDRFNKEVYGDWWDTGDWGLMNEEGNLFLHDRQVDLIDKI
CCCCEEEEEECCCEEEEECCCCCCCHHHCCCCCCCCCCCEECCCCCEEEECCCHHHHHHH
ESNLAIEDLLLDQHDFLDEVVIIRDKSGKPQPILALADNAEMDWSAWWASIVDLPFLNEP
HHCCCHHHHHHCCHHCCCCEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCC
ILMAYDEIPRTATMKVQRLALERDLAK
CEEEHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFKHEPLNLYLNFKKSAEQFPYSPIIFDQTVGAFPELALETTYQKTHQAIVDKASLLSE
CCCCCCCEEEEEEECHHHHHCCCCCEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGIKKSDKVIIFKSSAFDTYLLAVAVSYLGAVPAMISYHLPAETMDILAGRLDDPWLIYD
HCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEE
QVTDQTVTEMKNLKNSKKLSVSKFASQKPSHLVDSQQLDNDEIAYLTHTSGTTGVPKLIA
CCCHHHHHHHHHCCCCCCCHHHHHHCCCCCHHCCHHCCCCCCEEEEEECCCCCCHHHHHH
HSANSMGWRWVLQRTVMDWMPDKKEILAFHISPVHSRFNIGVSSAMTFGFGLMPLNDLSR
HCCCCCHHHHHHHHHHHHHCCCHHHEEEEEECCCCHHHCCCHHHHHHHCCCCCCCCCCCH
INLVNLFSKHQPYAFETHPNDFVRLANLAKQEPEIFSSIRYLHSTFDAINKETMHTFLSA
HHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKHDNPVFLQIYGQSECGPMIWKKHRLSTLADTDAREMGIGMPGLSKARIADENGNECPA
CCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEECCCCCCCCC
GIPGRIHFLSKGRALTYYKEEDRFNKEVYGDWWDTGDWGLMNEEGNLFLHDRQVDLIDKI
CCCCEEEEEECCCEEEEECCCCCCCHHHCCCCCCCCCCCEECCCCCEEEECCCHHHHHHH
ESNLAIEDLLLDQHDFLDEVVIIRDKSGKPQPILALADNAEMDWSAWWASIVDLPFLNEP
HHCCCHHHHHHCCHHCCCCEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCC
ILMAYDEIPRTATMKVQRLALERDLAK
CEEEHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]