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The map label for this gene is holB [H]

Identifier: 125623283

GI number: 125623283

Start: 409880

End: 410740

Strand: Direct

Name: holB [H]

Synonym: llmg_0416

Alternate gene names: 125623283

Gene position: 409880-410740 (Clockwise)

Preceding gene: 125623282

Following gene: 125623284

Centisome position: 16.2

GC content: 33.1

Gene sequence:

>861_bases
GTGGAAATAGTAGATATTCAGCCTCAACTTTATAAGCAATTTTCAACAATTTTGCAACATGGAAAATTAAGCCATGCTTA
TTTATTTTCAGGTGGATTTGGTTCTTTTGAATTAGCAATTTGGTTGAGCCAAGCCCTATTTTGTGAGAATCCTGAGAATT
CTCTTCCTTGTGGGCATTGTCGTTCTTGTCGTCTGGTTGCTCAACAGGAATTTGCCGATTTGCACATTGTTGAACCAGAT
GGACAGACGATAAAAACAGCACAAATTCGTGATTTGACACAGGTTTTTAGTGAATCTGGTTATGAAGGAAAGCAGCAAGT
TTTGTTAATTAGGGAAGCAGAAAAAATGCATCCTAATGCGGCAAATGCTCTATTGAAATCTATTGAAGAACCTGAGACAG
AAATTGTTGTTTTTTTACTGACAGATAATGAGAACATGATTTTGCAAACGATAAAATCACGGACACAGATTATTAATTTC
CGGAAAAATGTTCAATTTTTACAAGATTTTCTTGAAAAAAATGGAATTTTAAAAACGCAAGCAGAACTCCTAGCTGAAAT
TTGTAATTCTACTGACAGAGCTTTAGAATTGGCCCAACAAAGTTGGTTCAATGAAGGACTTCAGAGAATTCAACATTTTG
TTAAACTTCTAAAAAAATCAGCTGACGAGGCCTTTTTATACTTGAAAGATTTGGTTGAAATTTTTGATGATAAAGAAAAG
CAAAATCAAGCTTTTGAACTTTTATTGCAACTATTTAATCAAGAAAGAATGAGTCAAGAACTTCTAAAAACCTTTCAGGC
AATTAAAATGTGGAAAAGTAATGTTCGATTTGAGTCAAGTTTAACTTTTATTGTTCTGTAA

Upstream 100 bases:

>100_bases
GTGATAAATACTGACAAAAATAAAAAATACGTAGATGTCAAAAAAATTTTTTTATTTTGTTATTTAAAATAGATTTGGTA
AATATTTAGAAAGGTTTCTT

Downstream 100 bases:

>100_bases
AACTTGGTCAGCAACCCTTTAATCAGAAATCTTATATCAGGAGAATAAAATGATTTATGAAATAAAATTTGCGCATGGTG
AATCTAATATATTTGCAACA

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MEIVDIQPQLYKQFSTILQHGKLSHAYLFSGGFGSFELAIWLSQALFCENPENSLPCGHCRSCRLVAQQEFADLHIVEPD
GQTIKTAQIRDLTQVFSESGYEGKQQVLLIREAEKMHPNAANALLKSIEEPETEIVVFLLTDNENMILQTIKSRTQIINF
RKNVQFLQDFLEKNGILKTQAELLAEICNSTDRALELAQQSWFNEGLQRIQHFVKLLKKSADEAFLYLKDLVEIFDDKEK
QNQAFELLLQLFNQERMSQELLKTFQAIKMWKSNVRFESSLTFIVL

Sequences:

>Translated_286_residues
MEIVDIQPQLYKQFSTILQHGKLSHAYLFSGGFGSFELAIWLSQALFCENPENSLPCGHCRSCRLVAQQEFADLHIVEPD
GQTIKTAQIRDLTQVFSESGYEGKQQVLLIREAEKMHPNAANALLKSIEEPETEIVVFLLTDNENMILQTIKSRTQIINF
RKNVQFLQDFLEKNGILKTQAELLAEICNSTDRALELAQQSWFNEGLQRIQHFVKLLKKSADEAFLYLKDLVEIFDDKEK
QNQAFELLLQLFNQERMSQELLKTFQAIKMWKSNVRFESSLTFIVL
>Mature_286_residues
MEIVDIQPQLYKQFSTILQHGKLSHAYLFSGGFGSFELAIWLSQALFCENPENSLPCGHCRSCRLVAQQEFADLHIVEPD
GQTIKTAQIRDLTQVFSESGYEGKQQVLLIREAEKMHPNAANALLKSIEEPETEIVVFLLTDNENMILQTIKSRTQIINF
RKNVQFLQDFLEKNGILKTQAELLAEICNSTDRALELAQQSWFNEGLQRIQHFVKLLKKSADEAFLYLKDLVEIFDDKEK
QNQAFELLLQLFNQERMSQELLKTFQAIKMWKSNVRFESSLTFIVL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=136, Percent_Identity=36.0294117647059, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787341, Length=157, Percent_Identity=31.8471337579618, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 33053; Mature: 33053

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIVDIQPQLYKQFSTILQHGKLSHAYLFSGGFGSFELAIWLSQALFCENPENSLPCGHC
CCEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
RSCRLVAQQEFADLHIVEPDGQTIKTAQIRDLTQVFSESGYEGKQQVLLIREAEKMHPNA
HHHHHHHHHHHCCEEEECCCCCEEHHHHHHHHHHHHHCCCCCCCHHEEEEECHHHHCCCH
ANALLKSIEEPETEIVVFLLTDNENMILQTIKSRTQIINFRKNVQFLQDFLEKNGILKTQ
HHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AELLAEICNSTDRALELAQQSWFNEGLQRIQHFVKLLKKSADEAFLYLKDLVEIFDDKEK
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHH
QNQAFELLLQLFNQERMSQELLKTFQAIKMWKSNVRFESSLTFIVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCEEEEEC
>Mature Secondary Structure
MEIVDIQPQLYKQFSTILQHGKLSHAYLFSGGFGSFELAIWLSQALFCENPENSLPCGHC
CCEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
RSCRLVAQQEFADLHIVEPDGQTIKTAQIRDLTQVFSESGYEGKQQVLLIREAEKMHPNA
HHHHHHHHHHHCCEEEECCCCCEEHHHHHHHHHHHHHCCCCCCCHHEEEEECHHHHCCCH
ANALLKSIEEPETEIVVFLLTDNENMILQTIKSRTQIINFRKNVQFLQDFLEKNGILKTQ
HHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AELLAEICNSTDRALELAQQSWFNEGLQRIQHFVKLLKKSADEAFLYLKDLVEIFDDKEK
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHH
QNQAFELLLQLFNQERMSQELLKTFQAIKMWKSNVRFESSLTFIVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]