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The map label for this gene is ppnK [H]

Identifier: 125623252

GI number: 125623252

Start: 374551

End: 375363

Strand: Direct

Name: ppnK [H]

Synonym: llmg_0383

Alternate gene names: 125623252

Gene position: 374551-375363 (Clockwise)

Preceding gene: 125623251

Following gene: 125623253

Centisome position: 14.81

GC content: 38.01

Gene sequence:

>813_bases
ATGAACTTTGGTAAAAAAGTCTGGCTAATTGGAAATTCAAGTGAGAAGTCTAAAAAAACACTCAATAAATTGAGCAAGAT
TTTGAAAGCTGAACATTTTGTTTTTGATGATATTAATCCTGAAATTGTGATTTCAGTTGGAGGCGATGGAACGCTTCTAC
GGGCCATGCATATGTACGAGTATCAACTAGACCGGGTACGCTTTTTAGGTGTTCATACGGGACATTTGGGCTTTTATACA
GATTTTACTGATGAAGATTTATTTGAAGTCGTTGAAGCACTTTATGACGAAAATCCAGCTCAAGCCATTCACTACCCACT
CATTTGTGTTCAAGTTAGTTTTACTGATGGCTATCAAATTGTTCGTCATGTGCTTAATGAAGCAACCATTCGGCGTGCAA
GTAAGACAATGGTGGGTGATGTCAGGATTTCTGATTATCTTTTTGAACGCTTCCGGGGGGATGGTTTATCTATTTCTACT
CCAACTGGTTCAACGGCTTATAATAAATCAATTGGGGGGGCAGTAGTCCATCCACGTGTGAAGGCCATGCAAATTGCTGA
AATTGCGAGTTTGAATAATGTTGTTTATCGGACTTTAGGTTCACCAATGATTGTGGCTGAGAAAGATACAATTACGGTTT
GTCCTGCGCCAGAAGATGATTATAGTTTAACTTTTGACCAATTAACTTTTGAATATAAAAATATTAAGTCAATTGAGTTT
TCTTTAGATGGAACAACGATTTCTTTTGCTAATTGCGCCCATACACCATTTTGGGAACGGGTCAGTAAATCCTTCATCGG
GGAGGTTGAATAA

Upstream 100 bases:

>100_bases
TCTGAAATGGGCGAAATTCGGGAAGATATTCAAGAAGCACAGTTACTTTTTGATAGCCCTCAGCAAAAACAAGACTTTAA
AAAAGAAAATGGTGAAGAAA

Downstream 100 bases:

>100_bases
TGCAGTTTGCTTTTAGAAACGAGATTGAAGGAAGCATGGTCAAATCAATGCTAGGCCGTCATGGTATTTCTAGGAGATTA
CTGACAAAAGTTAAATTTGA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MNFGKKVWLIGNSSEKSKKTLNKLSKILKAEHFVFDDINPEIVISVGGDGTLLRAMHMYEYQLDRVRFLGVHTGHLGFYT
DFTDEDLFEVVEALYDENPAQAIHYPLICVQVSFTDGYQIVRHVLNEATIRRASKTMVGDVRISDYLFERFRGDGLSIST
PTGSTAYNKSIGGAVVHPRVKAMQIAEIASLNNVVYRTLGSPMIVAEKDTITVCPAPEDDYSLTFDQLTFEYKNIKSIEF
SLDGTTISFANCAHTPFWERVSKSFIGEVE

Sequences:

>Translated_270_residues
MNFGKKVWLIGNSSEKSKKTLNKLSKILKAEHFVFDDINPEIVISVGGDGTLLRAMHMYEYQLDRVRFLGVHTGHLGFYT
DFTDEDLFEVVEALYDENPAQAIHYPLICVQVSFTDGYQIVRHVLNEATIRRASKTMVGDVRISDYLFERFRGDGLSIST
PTGSTAYNKSIGGAVVHPRVKAMQIAEIASLNNVVYRTLGSPMIVAEKDTITVCPAPEDDYSLTFDQLTFEYKNIKSIEF
SLDGTTISFANCAHTPFWERVSKSFIGEVE
>Mature_270_residues
MNFGKKVWLIGNSSEKSKKTLNKLSKILKAEHFVFDDINPEIVISVGGDGTLLRAMHMYEYQLDRVRFLGVHTGHLGFYT
DFTDEDLFEVVEALYDENPAQAIHYPLICVQVSFTDGYQIVRHVLNEATIRRASKTMVGDVRISDYLFERFRGDGLSIST
PTGSTAYNKSIGGAVVHPRVKAMQIAEIASLNNVVYRTLGSPMIVAEKDTITVCPAPEDDYSLTFDQLTFEYKNIKSIEF
SLDGTTISFANCAHTPFWERVSKSFIGEVE

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788968, Length=157, Percent_Identity=29.2993630573248, Blast_Score=62, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320794, Length=192, Percent_Identity=29.1666666666667, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322509, Length=175, Percent_Identity=30.2857142857143, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325068, Length=153, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 30343; Mature: 30343

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFGKKVWLIGNSSEKSKKTLNKLSKILKAEHFVFDDINPEIVISVGGDGTLLRAMHMYE
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCHHHHHHHHHH
YQLDRVRFLGVHTGHLGFYTDFTDEDLFEVVEALYDENPAQAIHYPLICVQVSFTDGYQI
HHHHHEEEEEEEECCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCHHH
VRHVLNEATIRRASKTMVGDVRISDYLFERFRGDGLSISTPTGSTAYNKSIGGAVVHPRV
HHHHHHHHHHHHHHHHEECCEEHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEEECCCH
KAMQIAEIASLNNVVYRTLGSPMIVAEKDTITVCPAPEDDYSLTFDQLTFEYKNIKSIEF
HHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEECCCCCCCCEEEHHHEEEEECCCEEEEE
SLDGTTISFANCAHTPFWERVSKSFIGEVE
EECCCEEEECCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNFGKKVWLIGNSSEKSKKTLNKLSKILKAEHFVFDDINPEIVISVGGDGTLLRAMHMYE
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCHHHHHHHHHH
YQLDRVRFLGVHTGHLGFYTDFTDEDLFEVVEALYDENPAQAIHYPLICVQVSFTDGYQI
HHHHHEEEEEEEECCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCHHH
VRHVLNEATIRRASKTMVGDVRISDYLFERFRGDGLSISTPTGSTAYNKSIGGAVVHPRV
HHHHHHHHHHHHHHHHEECCEEHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEEECCCH
KAMQIAEIASLNNVVYRTLGSPMIVAEKDTITVCPAPEDDYSLTFDQLTFEYKNIKSIEF
HHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEECCCCCCCCEEEHHHEEEEECCCEEEEE
SLDGTTISFANCAHTPFWERVSKSFIGEVE
EECCCEEEECCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]