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The map label for this gene is rfbX [H]

Identifier: 125623102

GI number: 125623102

Start: 213980

End: 215398

Strand: Direct

Name: rfbX [H]

Synonym: llmg_0225

Alternate gene names: 125623102

Gene position: 213980-215398 (Clockwise)

Preceding gene: 125623101

Following gene: 125623103

Centisome position: 8.46

GC content: 32.56

Gene sequence:

>1419_bases
TTGAAAGTATTAAAAAACTATCTTCTGAATAGCTCTTACCAATTATTGATTGTGATTATTCCAATTATCACGATTCCTTA
TATTTCTAGGGTGCTTGGCACAACGGCTATAGGTTTAAATACATTTACCTATGCAATTATTCAGTACTTTGTATTAGCTG
GTTCAATTGGGATAACAACTTATGGAAATCGAGAGATTGCATATCATCAATCTGATAAAGAAAAGAGAAGCCAAATTTTT
TGGGAAATTTCATTTTTGAGATTTTGTACCATTGCTCTCTCTTTTCTTATTTTCTGTATTTTCTTAGCCTTTCAAAAACA
GGATTTTGAGATTTATCTCTTGCAGAGTATCGCTATTATCGCTGCGGCTTTTGATATTTCTTGGTATTTTATGGGAGTGG
AAAATTTCAAGCGAACAGTAGGGCGTAATTTTATTGTCTCTATTATTTCAGTTATTTTCATCTTTACTTTTATTAAGAGT
CCAAAGGATTTGCCCATTTATGTTTTAATCATTACTGGAACATCATTGATTGGAAATCTTTCACTTTGGCCATATTTGCG
CAAGGAAATTTTCGCTCCCAAATGGAAAGAATTAGCATTAGGACATCATTTAAAACCCACACTATTACTTTTTTTGCCGC
AAATTGCTACACAGATTTATACAATTGCAAACAAAACGATGATTGGGATTTTTGATGGGAAAACGGCATCGGGATTTTTT
AGTCAATCGGATAGTCTGATTAAAGTAACATTGAGCATTGTCACTTCTTTAGGTGTAGTCATGTTGCCTCATGTTTCAAA
TCTTTTTTCAAAAGGTAAAATTAAAGAAGTTCAAGAGACTTTAAAAAAATCTTTCGTCCTTATGACTGGGCTTGCTGTAC
CGATAATGTTTGGCGTTATGGGAATAGCACTCAATTTTGCAGGATTCTTTTTCGGCCCTAAATGGGTAGCTGTTGGTCCT
TTGCTAATGATGGAAGCCCCAATTATTATATTCATTGCTTGGAGTAATGTTTTGGGAATTCAGTACCTTCTTCCATTAAA
TCGGATGAGAGAATTCACAACTTCGGTAACAATTGGTGCAGTTCTTAATATTTTACTTAATTTTGCTTTGATTCCTCTTT
TGGGGCTCACTGGTGCAATGATTGCGACAGTAATTGCCGAGGCTTCAGTGACGATTTATCAATTTTATATTTTGAGAAAT
GACTTTGAGATAATTCCAATGATTTTATCTTGCTGGAAATACTTTTTATCTGGTGCAGTGATGTTTGGAGCTGTTTTCTA
TTTAAACAATTCTTTGAAGATGAATATGCATAATCTTATATTTCAAGTATTGATTGGTGCTATAATATATATAATTCTTA
ATATCTTATTGAAATCCAGTCTTTTTATTGAAGTCAAAAAGATTGTAAGTAAAAAATAA

Upstream 100 bases:

>100_bases
AAACAGTAATATTTGGTGGTCGTTTGGGTAACTATCAGTATTATGATATGGACCAAGTCTTTAATGCAGCTTTAAAAGCT
GTCGAAAAGGAATTTAAAGA

Downstream 100 bases:

>100_bases
CCCTAATACTAAACGATTGGAGAATAAAATGTATAAAAAGGTAACCTTCTTAGTTACTCTTATTGGAGTGATGGGGCTAT
TACTCTTTACTTTCTATGAA

Product: putative O-antigen transporter

Products: lipopolysaccharide [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MKVLKNYLLNSSYQLLIVIIPIITIPYISRVLGTTAIGLNTFTYAIIQYFVLAGSIGITTYGNREIAYHQSDKEKRSQIF
WEISFLRFCTIALSFLIFCIFLAFQKQDFEIYLLQSIAIIAAAFDISWYFMGVENFKRTVGRNFIVSIISVIFIFTFIKS
PKDLPIYVLIITGTSLIGNLSLWPYLRKEIFAPKWKELALGHHLKPTLLLFLPQIATQIYTIANKTMIGIFDGKTASGFF
SQSDSLIKVTLSIVTSLGVVMLPHVSNLFSKGKIKEVQETLKKSFVLMTGLAVPIMFGVMGIALNFAGFFFGPKWVAVGP
LLMMEAPIIIFIAWSNVLGIQYLLPLNRMREFTTSVTIGAVLNILLNFALIPLLGLTGAMIATVIAEASVTIYQFYILRN
DFEIIPMILSCWKYFLSGAVMFGAVFYLNNSLKMNMHNLIFQVLIGAIIYIILNILLKSSLFIEVKKIVSKK

Sequences:

>Translated_472_residues
MKVLKNYLLNSSYQLLIVIIPIITIPYISRVLGTTAIGLNTFTYAIIQYFVLAGSIGITTYGNREIAYHQSDKEKRSQIF
WEISFLRFCTIALSFLIFCIFLAFQKQDFEIYLLQSIAIIAAAFDISWYFMGVENFKRTVGRNFIVSIISVIFIFTFIKS
PKDLPIYVLIITGTSLIGNLSLWPYLRKEIFAPKWKELALGHHLKPTLLLFLPQIATQIYTIANKTMIGIFDGKTASGFF
SQSDSLIKVTLSIVTSLGVVMLPHVSNLFSKGKIKEVQETLKKSFVLMTGLAVPIMFGVMGIALNFAGFFFGPKWVAVGP
LLMMEAPIIIFIAWSNVLGIQYLLPLNRMREFTTSVTIGAVLNILLNFALIPLLGLTGAMIATVIAEASVTIYQFYILRN
DFEIIPMILSCWKYFLSGAVMFGAVFYLNNSLKMNMHNLIFQVLIGAIIYIILNILLKSSLFIEVKKIVSKK
>Mature_472_residues
MKVLKNYLLNSSYQLLIVIIPIITIPYISRVLGTTAIGLNTFTYAIIQYFVLAGSIGITTYGNREIAYHQSDKEKRSQIF
WEISFLRFCTIALSFLIFCIFLAFQKQDFEIYLLQSIAIIAAAFDISWYFMGVENFKRTVGRNFIVSIISVIFIFTFIKS
PKDLPIYVLIITGTSLIGNLSLWPYLRKEIFAPKWKELALGHHLKPTLLLFLPQIATQIYTIANKTMIGIFDGKTASGFF
SQSDSLIKVTLSIVTSLGVVMLPHVSNLFSKGKIKEVQETLKKSFVLMTGLAVPIMFGVMGIALNFAGFFFGPKWVAVGP
LLMMEAPIIIFIAWSNVLGIQYLLPLNRMREFTTSVTIGAVLNILLNFALIPLLGLTGAMIATVIAEASVTIYQFYILRN
DFEIIPMILSCWKYFLSGAVMFGAVFYLNNSLKMNMHNLIFQVLIGAIIYIILNILLKSSLFIEVKKIVSKK

Specific function: May be involved in the translocation process of the nascent O-polysaccharide molecules and/or its ligation to lipid A core units [H]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788349, Length=396, Percent_Identity=22.7272727272727, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002797 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 53148; Mature: 53148

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLKNYLLNSSYQLLIVIIPIITIPYISRVLGTTAIGLNTFTYAIIQYFVLAGSIGITT
CHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
YGNREIAYHQSDKEKRSQIFWEISFLRFCTIALSFLIFCIFLAFQKQDFEIYLLQSIAII
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHH
AAAFDISWYFMGVENFKRTVGRNFIVSIISVIFIFTFIKSPKDLPIYVLIITGTSLIGNL
HHHHHHHHEEEEHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHCCC
SLWPYLRKEIFAPKWKELALGHHLKPTLLLFLPQIATQIYTIANKTMIGIFDGKTASGFF
HHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCC
SQSDSLIKVTLSIVTSLGVVMLPHVSNLFSKGKIKEVQETLKKSFVLMTGLAVPIMFGVM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIALNFAGFFFGPKWVAVGPLLMMEAPIIIFIAWSNVLGIQYLLPLNRMREFTTSVTIGA
HHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
VLNILLNFALIPLLGLTGAMIATVIAEASVTIYQFYILRNDFEIIPMILSCWKYFLSGAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHEEEEEHHCCHHHHHHHHHHHHHHHHHHH
MFGAVFYLNNSLKMNMHNLIFQVLIGAIIYIILNILLKSSLFIEVKKIVSKK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVLKNYLLNSSYQLLIVIIPIITIPYISRVLGTTAIGLNTFTYAIIQYFVLAGSIGITT
CHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
YGNREIAYHQSDKEKRSQIFWEISFLRFCTIALSFLIFCIFLAFQKQDFEIYLLQSIAII
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHH
AAAFDISWYFMGVENFKRTVGRNFIVSIISVIFIFTFIKSPKDLPIYVLIITGTSLIGNL
HHHHHHHHEEEEHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHCCC
SLWPYLRKEIFAPKWKELALGHHLKPTLLLFLPQIATQIYTIANKTMIGIFDGKTASGFF
HHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCC
SQSDSLIKVTLSIVTSLGVVMLPHVSNLFSKGKIKEVQETLKKSFVLMTGLAVPIMFGVM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIALNFAGFFFGPKWVAVGPLLMMEAPIIIFIAWSNVLGIQYLLPLNRMREFTTSVTIGA
HHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
VLNILLNFALIPLLGLTGAMIATVIAEASVTIYQFYILRNDFEIIPMILSCWKYFLSGAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHEEEEEHHCCHHHHHHHHHHHHHHHHHHH
MFGAVFYLNNSLKMNMHNLIFQVLIGAIIYIILNILLKSSLFIEVKKIVSKK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipopolysaccharide [Cytoplasm] [C]

Specific reaction: lipopolysaccharide [Cytoplasm] = lipopolysaccharide [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7517390; 7517391; 9097040; 9278503 [H]