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The map label for this gene is aroA

Identifier: 124486462

GI number: 124486462

Start: 1677995

End: 1679263

Strand: Reverse

Name: aroA

Synonym: Mlab_1650

Alternate gene names: 124486462

Gene position: 1679263-1677995 (Counterclockwise)

Preceding gene: 124486463

Following gene: 124486461

Centisome position: 93.04

GC content: 54.14

Gene sequence:

>1269_bases
ATGAAACTTATCGTATCTCGTTCTCAAATCTCCGGATGTGTCCATGCACCTCCCTCAAAAAGTCACACACACCGGGCATT
TCTCCTTGCGTCTCTTGCAAAAGGAGAATCGGTCGTTCTCTCTCCGCTTCTGGGCGAGGACACTCTGGCTACTCTCTCCG
CGGTCAAAGCGCTTGGAGCGAACGTATGCGAAGGGGATGATCGAATCACGATCCAGGGCGGCAATTTACACGCCCCGCTC
CCGAAAGGAACGGTGATAAACTGTAAAAACTCGGGCACCTCCATTCGGATGCTTGCAGGCATAGCTTCCCGTCTGGATGG
AACGACTGAGTTCACGGGTGACGCTTCGCTCTGTTCCCGCCCTATGAAGCCTCTGCTTGACGCCCTGTCAGAACTTGGAG
CCGGGGTAACATCCGACAACGGATGTGCTCCGTTCACCATAACGGGTCCGGTATCGGGCGGCGATGTCCATATTCGCGGT
GACGTGAGTTCTCAATTCATCTCCGGCCTGCTGATCTCTGCTCCGCTTGGCAAAGCTGACACGAGGATCCACCTGACAAC
TCCCCTCACGTCAAAACCATACGTGGACATGACGATTTCTGCTATGAAAAAGCACGGCGTTTCGGTCGAGACGATCGAAG
ATGGATATCTTGTCCGTTCAGGTCAGGTCTATTCTTCCGAGGATGTTCAGGTTGGCGGCGACTACTCGTCGGCCGCATTT
CTGTTTGCGGCGGCGGCACTCGCCGGGGAGATCGCCGTTTCCGGACTCGACCCGGCTGACCCTCAGGGCGATCAGGTTGT
GATCTCCATCCTTGAAACATTCGGGGCAGGAGTAGTTCGTGATGGCGAAAACGTTACGATTCGAAAAGCAGCTTTGAAGG
CTGCAGACATCGATCTTGCGAACGCTCCGGATCTGTTTCCCATTATCGCGGTCCTTGCGTCGCAGGCGAAAGGCACCAGC
AGATTATACGGCGCCGCTCATCTCAGATTCAAGGAAAGCGACCGTATCATGTCCACGGTCCTTTTCCTCAGATCGATGGG
TGCAGATATCAGCGAGACTGAGGATGGATGCATTGTTACGGGACCTGCCAATCTTTCCGGGGCAAATGTTACTACATTTG
GCGACCACCGTATAATGATGGCATCAGCGGTTGCCGGGCTTATCGCAGATAGTACTACGACCGTAGATGATGCCGGCTGC
TGCGCAGTTTCCTATCCGGGTTTTGTGAAAGATATGCAGAAACTCGGTGCGGATATGAGGGAAGAATGA

Upstream 100 bases:

>100_bases
GCCACCGGGATCCTTGTGCCGGATGAAGGCCTTGACGAATTTCTCGATCGGTTCGGTCGGGAGAATGTTCTCCTCGCGAA
CATCAGGAACGGTGTGTAAG

Downstream 100 bases:

>100_bases
ATCGAATCGGAGAATCAATAACGCTCACGTTGTTTGGGGCGAGTCACGACAGTCGTATCGGCTGTGTTATAGACGGAATT
CCTCCGGGCTATCCTGTAAA

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKLIVSRSQISGCVHAPPSKSHTHRAFLLASLAKGESVVLSPLLGEDTLATLSAVKALGANVCEGDDRITIQGGNLHAPL
PKGTVINCKNSGTSIRMLAGIASRLDGTTEFTGDASLCSRPMKPLLDALSELGAGVTSDNGCAPFTITGPVSGGDVHIRG
DVSSQFISGLLISAPLGKADTRIHLTTPLTSKPYVDMTISAMKKHGVSVETIEDGYLVRSGQVYSSEDVQVGGDYSSAAF
LFAAAALAGEIAVSGLDPADPQGDQVVISILETFGAGVVRDGENVTIRKAALKAADIDLANAPDLFPIIAVLASQAKGTS
RLYGAAHLRFKESDRIMSTVLFLRSMGADISETEDGCIVTGPANLSGANVTTFGDHRIMMASAVAGLIADSTTTVDDAGC
CAVSYPGFVKDMQKLGADMREE

Sequences:

>Translated_422_residues
MKLIVSRSQISGCVHAPPSKSHTHRAFLLASLAKGESVVLSPLLGEDTLATLSAVKALGANVCEGDDRITIQGGNLHAPL
PKGTVINCKNSGTSIRMLAGIASRLDGTTEFTGDASLCSRPMKPLLDALSELGAGVTSDNGCAPFTITGPVSGGDVHIRG
DVSSQFISGLLISAPLGKADTRIHLTTPLTSKPYVDMTISAMKKHGVSVETIEDGYLVRSGQVYSSEDVQVGGDYSSAAF
LFAAAALAGEIAVSGLDPADPQGDQVVISILETFGAGVVRDGENVTIRKAALKAADIDLANAPDLFPIIAVLASQAKGTS
RLYGAAHLRFKESDRIMSTVLFLRSMGADISETEDGCIVTGPANLSGANVTTFGDHRIMMASAVAGLIADSTTTVDDAGC
CAVSYPGFVKDMQKLGADMREE
>Mature_422_residues
MKLIVSRSQISGCVHAPPSKSHTHRAFLLASLAKGESVVLSPLLGEDTLATLSAVKALGANVCEGDDRITIQGGNLHAPL
PKGTVINCKNSGTSIRMLAGIASRLDGTTEFTGDASLCSRPMKPLLDALSELGAGVTSDNGCAPFTITGPVSGGDVHIRG
DVSSQFISGLLISAPLGKADTRIHLTTPLTSKPYVDMTISAMKKHGVSVETIEDGYLVRSGQVYSSEDVQVGGDYSSAAF
LFAAAALAGEIAVSGLDPADPQGDQVVISILETFGAGVVRDGENVTIRKAALKAADIDLANAPDLFPIIAVLASQAKGTS
RLYGAAHLRFKESDRIMSTVLFLRSMGADISETEDGCIVTGPANLSGANVTTFGDHRIMMASAVAGLIADSTTTVDDAGC
CAVSYPGFVKDMQKLGADMREE

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=418, Percent_Identity=34.4497607655502, Blast_Score=208, Evalue=6e-55,
Organism=Saccharomyces cerevisiae, GI6320332, Length=459, Percent_Identity=27.8867102396514, Blast_Score=128, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_METLZ (A2SU05)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001031078.1
- ProteinModelPortal:   A2SU05
- SMR:   A2SU05
- STRING:   A2SU05
- GeneID:   4795407
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_1650
- eggNOG:   arNOG05032
- HOGENOM:   HBG646626
- OMA:   SSQFITS
- PhylomeDB:   A2SU05
- BioCyc:   MLAB410358:MLAB_1650-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 43626; Mature: 43626

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIVSRSQISGCVHAPPSKSHTHRAFLLASLAKGESVVLSPLLGEDTLATLSAVKALGA
CEEEEEHHHHCCCEECCCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCC
NVCEGDDRITIQGGNLHAPLPKGTVINCKNSGTSIRMLAGIASRLDGTTEFTGDASLCSR
CCCCCCCEEEEECCCEECCCCCCCEEEECCCCCEEHHHHHHHHHCCCCCEECCCHHHHHC
PMKPLLDALSELGAGVTSDNGCAPFTITGPVSGGDVHIRGDVSSQFISGLLISAPLGKAD
CHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCEEEEECCCHHHHHHHEEEECCCCCCC
TRIHLTTPLTSKPYVDMTISAMKKHGVSVETIEDGYLVRSGQVYSSEDVQVGGDYSSAAF
CEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCEEEECCCEECCCCCEECCCCCHHHH
LFAAAALAGEIAVSGLDPADPQGDQVVISILETFGAGVVRDGENVTIRKAALKAADIDLA
HHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHCCCEEECCCCEEEEHHHHHHHCCCCC
NAPDLFPIIAVLASQAKGTSRLYGAAHLRFKESDRIMSTVLFLRSMGADISETEDGCIVT
CCCCHHHHHHHHHHCCCCCHHEEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCEEEE
GPANLSGANVTTFGDHRIMMASAVAGLIADSTTTVDDAGCCAVSYPGFVKDMQKLGADMR
CCCCCCCCEEEEECCCEEHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCC
EE
CC
>Mature Secondary Structure
MKLIVSRSQISGCVHAPPSKSHTHRAFLLASLAKGESVVLSPLLGEDTLATLSAVKALGA
CEEEEEHHHHCCCEECCCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCC
NVCEGDDRITIQGGNLHAPLPKGTVINCKNSGTSIRMLAGIASRLDGTTEFTGDASLCSR
CCCCCCCEEEEECCCEECCCCCCCEEEECCCCCEEHHHHHHHHHCCCCCEECCCHHHHHC
PMKPLLDALSELGAGVTSDNGCAPFTITGPVSGGDVHIRGDVSSQFISGLLISAPLGKAD
CHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCEEEEECCCHHHHHHHEEEECCCCCCC
TRIHLTTPLTSKPYVDMTISAMKKHGVSVETIEDGYLVRSGQVYSSEDVQVGGDYSSAAF
CEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCEEEECCCEECCCCCEECCCCCHHHH
LFAAAALAGEIAVSGLDPADPQGDQVVISILETFGAGVVRDGENVTIRKAALKAADIDLA
HHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHCCCEEECCCCEEEEHHHHHHHCCCCC
NAPDLFPIIAVLASQAKGTSRLYGAAHLRFKESDRIMSTVLFLRSMGADISETEDGCIVT
CCCCHHHHHHHHHHCCCCCHHEEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCEEEE
GPANLSGANVTTFGDHRIMMASAVAGLIADSTTTVDDAGCCAVSYPGFVKDMQKLGADMR
CCCCCCCCEEEEECCCEEHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCC
EE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA