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The map label for this gene is cofG [H]

Identifier: 124486404

GI number: 124486404

Start: 1620963

End: 1621928

Strand: Reverse

Name: cofG [H]

Synonym: Mlab_1590

Alternate gene names: 124486404

Gene position: 1621928-1620963 (Counterclockwise)

Preceding gene: 124486405

Following gene: 124486403

Centisome position: 89.86

GC content: 56.11

Gene sequence:

>966_bases
ATGCCGGTCATTACGTTTTCCCGCAACGTGTTTCTTCCGCTGACCAACGTCTGTGCAAATACCTGCGGATACTGCTCGTT
CAAATCCCCGGTGGCCGAAGGCTGCGTGATGCCAAAGGAGGAGGTTGTCTCCACGCTCGAACGCGGGGCGGCGTTTTCCT
GCACCGAGGCCCTTTTCACGTTCGGCGAACGTCCCGAACGCGAAGCAGGTTTCACCGCATACATTAACCGGATGGGGTAT
CCCGATATTCTTTCCTACTGTAAAGACATGAGCAGATACGCAATCTCGCTTGGGATTTTGCCGCACACGAATGCCGGCAT
CCTCACCTATGAGGAGCTGGAGGATCTCCGGCCCGTGAACGCGAGCATGGGTCTGATGCTTGAAACGACCGCCGAGATCC
CTGCCCATGCCCACAGTCCGGGAAAAGATCCGTCAGTCAGGATAGAAATGATGGAAAATGCGGGAAAACTCAAGATCCCG
TTCACCACCGGACTGCTGCTTGGGATCGGCGAAACCCGGGACGACCGTATCGAGTCGCTCGAAGTCATCCGCGATCTGCA
TAAAAAATTCGGGCACATCCAGGAAGTGATCGTCCAGAACTTCTGCCCGAAAGAAGGTACCGACATGGCGTCGTTTCAGG
GAGCTTCGACCGAGGTGATCGCGGATACCCTCCGGCTTTCTAAAGAGATCCTGCCCGCTGATGTGTCGATCCAGATCCCG
CCGAATCTTGCCGATGCTTCCCTCCTTCTTGATCTCGGCGTGACCGATCTTGGCGGAGTGTCGCCGGTTACGATCGATTA
CATCAATCCGGAACATCCCTGGCCGGCTCTGGATGCATTGAAGGACATTGCCCGCGGATACGAGGTACGGGAACGTCTGT
GCATTTATGAGAAATACTGCACCCCGGCGTGGGTGGCTCCGGAGTTGTTCGGACTCGTGAGCGAACTCGCGAAGAAGGTG
TACTGA

Upstream 100 bases:

>100_bases
GATTTGGGTATGTTCTCTATCTGATCGCCGGCATCTGCCTGATCGTTTCGCTTTTGTGTCTTGTTATCGGACGCCGTGTT
TTCAAAGAAGGGGCGTGATC

Downstream 100 bases:

>100_bases
TGACTGCTTGTGGGTGTGTCGTAACAATACTGAACATCTTTACCTCGCCGGCCCAATACTAATGTACATGGATGACGTCT
GTCCCGCCCCGGTGAAGGAA

Product: hypothetical protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 1 [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MPVITFSRNVFLPLTNVCANTCGYCSFKSPVAEGCVMPKEEVVSTLERGAAFSCTEALFTFGERPEREAGFTAYINRMGY
PDILSYCKDMSRYAISLGILPHTNAGILTYEELEDLRPVNASMGLMLETTAEIPAHAHSPGKDPSVRIEMMENAGKLKIP
FTTGLLLGIGETRDDRIESLEVIRDLHKKFGHIQEVIVQNFCPKEGTDMASFQGASTEVIADTLRLSKEILPADVSIQIP
PNLADASLLLDLGVTDLGGVSPVTIDYINPEHPWPALDALKDIARGYEVRERLCIYEKYCTPAWVAPELFGLVSELAKKV
Y

Sequences:

>Translated_321_residues
MPVITFSRNVFLPLTNVCANTCGYCSFKSPVAEGCVMPKEEVVSTLERGAAFSCTEALFTFGERPEREAGFTAYINRMGY
PDILSYCKDMSRYAISLGILPHTNAGILTYEELEDLRPVNASMGLMLETTAEIPAHAHSPGKDPSVRIEMMENAGKLKIP
FTTGLLLGIGETRDDRIESLEVIRDLHKKFGHIQEVIVQNFCPKEGTDMASFQGASTEVIADTLRLSKEILPADVSIQIP
PNLADASLLLDLGVTDLGGVSPVTIDYINPEHPWPALDALKDIARGYEVRERLCIYEKYCTPAWVAPELFGLVSELAKKV
Y
>Mature_320_residues
PVITFSRNVFLPLTNVCANTCGYCSFKSPVAEGCVMPKEEVVSTLERGAAFSCTEALFTFGERPEREAGFTAYINRMGYP
DILSYCKDMSRYAISLGILPHTNAGILTYEELEDLRPVNASMGLMLETTAEIPAHAHSPGKDPSVRIEMMENAGKLKIPF
TTGLLLGIGETRDDRIESLEVIRDLHKKFGHIQEVIVQNFCPKEGTDMASFQGASTEVIADTLRLSKEILPADVSIQIPP
NLADASLLLDLGVTDLGGVSPVTIDYINPEHPWPALDALKDIARGYEVRERLCIYEKYCTPAWVAPELFGLVSELAKKVY

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofG family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR019939
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 35353; Mature: 35222

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVITFSRNVFLPLTNVCANTCGYCSFKSPVAEGCVMPKEEVVSTLERGAAFSCTEALFT
CCEEEECCCEEEEHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHH
FGERPEREAGFTAYINRMGYPDILSYCKDMSRYAISLGILPHTNAGILTYEELEDLRPVN
CCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCCCCCCEEEHHHHHHCCCCC
ASMGLMLETTAEIPAHAHSPGKDPSVRIEMMENAGKLKIPFTTGLLLGIGETRDDRIESL
CCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEHHCEEEECCCCCHHHHHHH
EVIRDLHKKFGHIQEVIVQNFCPKEGTDMASFQGASTEVIADTLRLSKEILPADVSIQIP
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEC
PNLADASLLLDLGVTDLGGVSPVTIDYINPEHPWPALDALKDIARGYEVRERLCIYEKYC
CCCCCHHHHEECCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
TPAWVAPELFGLVSELAKKVY
CCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PVITFSRNVFLPLTNVCANTCGYCSFKSPVAEGCVMPKEEVVSTLERGAAFSCTEALFT
CEEEECCCEEEEHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHH
FGERPEREAGFTAYINRMGYPDILSYCKDMSRYAISLGILPHTNAGILTYEELEDLRPVN
CCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCCCCCCEEEHHHHHHCCCCC
ASMGLMLETTAEIPAHAHSPGKDPSVRIEMMENAGKLKIPFTTGLLLGIGETRDDRIESL
CCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEHHCEEEECCCCCHHHHHHH
EVIRDLHKKFGHIQEVIVQNFCPKEGTDMASFQGASTEVIADTLRLSKEILPADVSIQIP
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEC
PNLADASLLLDLGVTDLGGVSPVTIDYINPEHPWPALDALKDIARGYEVRERLCIYEKYC
CCCCCHHHHEECCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
TPAWVAPELFGLVSELAKKVY
CCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA