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The map label for this gene is mcrB [H]

Identifier: 124486380

GI number: 124486380

Start: 1597612

End: 1598925

Strand: Reverse

Name: mcrB [H]

Synonym: Mlab_1565

Alternate gene names: 124486380

Gene position: 1598925-1597612 (Counterclockwise)

Preceding gene: 124486390

Following gene: 124486379

Centisome position: 88.58

GC content: 51.22

Gene sequence:

>1314_bases
ATGGCAAAATACAAAGATGTAATCGACCTTTACGACGACAACGGCAAACTCTTAAAGAGCAATGTCGCACTCGAAAAGAT
CAGCCCGCTGGTTAACCCGGCAATTAAGAAAATCATCGACGACACCAAGAGGACCGTTGCGGTCAACCTTGGAGGCATGC
AGGATGCATTAAAGACCGGTAAGATCGGTAAGCACCAGCAGATTCTCGGTCGTGAACTCAACCTCGATATCGTCGGCAAC
ATTGACGCAATCGAAGCAAAAATCAAAGAGTACGTCTCTGTTGAAGCAGGCGACGACACGATGATTAAACGCTACAACGG
CGGAAAACTTCTGCTGGTCAAGGTGCCTTCTGCACGTATCGCAGCAGCAGCAACCTACGATGCAGCACTTACCTCTGTCG
CAGCAGCAACCACCTATGCAGTTGTCGAGCAGTTCAACGTTGACATGTTCGACGCAAACACCGTCAAAGCAGCAGCATTC
GGTACCTACCCGGTCACGATGGATCTCGCAGGCGGAGCATGTTCTATGATCATGTCTATCCCACAGAACAACGAGTCTCT
CGGATATGCACTTCGTAACATCCCGGCAAACCAGTCTGTGATGATCACTCACCGTAATGCAATGCAGGGTGCAGCACTTA
CTTCTGTCTTTGAACAGGCAGGAGAGTTTGAAATGGGCAGTGCAATCGGTCCGTTCGAGCGTGCACAGCTCTTACTTCTC
GCATACCAGGGTCTGAACGCAAACAACATCGTCTACGACCTTGTAAAAGAGAACGGCCAGACCGGTACCGTCGGTACTGT
TGTCCAGTCCTTAGTCGAGCGTGCGATCGAAGACAAAGTCATCACCCAGGGTGCAGCAGCTAAAGGCGGATACTTCAAGC
CCTACGAAACCAAAGACCCAATGCTTTGGAACGCATATACCTCTGCAGGAACTCTTGCAGCAACCATGGTCAACTGTGGT
GCCGGACGTTTCGCTCAGGCAGTATCATCAACCTTACTGTACTTCAACGACCTTATCGAACACGAAACCGGACTTCCGGG
CTCTGACTTCGGAAGAACGATGGGTGTTGCGGTCGGTTTCTCCTTCTTCAGCCACTCCATCTATGGTGGCGGAGGACCGG
GTATCTTCAACGGAAACCACGTCGTTACCCGTCACGCAGCAGGTGTTGGTATGCCCTGTATCGTTGCCGCTTGTGCACTT
GATGCAGGAACTCAGATGTTCTCTCCAGAAGGAACCGCCAAGATCTATGGTGAGACCTTCGGTCAGATCGACGAGTTTGC
CCACCCGCTCCAGGCAATTGCAAAAGCAGTTTAA

Upstream 100 bases:

>100_bases
GTCATCAATTATCAGTGATCCTATTAGCGACAATCAGCAATTTTCAGGAAAAGTAACGGTAAAACAGCCAATCAAATCAC
CTTACAAACGAGGAAAAATT

Downstream 100 bases:

>100_bases
ACCTGTATTGATTTCTGCGTAAGCGGAAAGGAATTAGAATGACAGAATCAACATACCCACAACTAAGAATCGTCCCCACG
AGATTCCTGAATCCGGAGAC

Product: methyl-coenzyme M reductase, beta subunit

Products: NA

Alternate protein names: Coenzyme-B sulfoethylthiotransferase beta [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MAKYKDVIDLYDDNGKLLKSNVALEKISPLVNPAIKKIIDDTKRTVAVNLGGMQDALKTGKIGKHQQILGRELNLDIVGN
IDAIEAKIKEYVSVEAGDDTMIKRYNGGKLLLVKVPSARIAAAATYDAALTSVAAATTYAVVEQFNVDMFDANTVKAAAF
GTYPVTMDLAGGACSMIMSIPQNNESLGYALRNIPANQSVMITHRNAMQGAALTSVFEQAGEFEMGSAIGPFERAQLLLL
AYQGLNANNIVYDLVKENGQTGTVGTVVQSLVERAIEDKVITQGAAAKGGYFKPYETKDPMLWNAYTSAGTLAATMVNCG
AGRFAQAVSSTLLYFNDLIEHETGLPGSDFGRTMGVAVGFSFFSHSIYGGGGPGIFNGNHVVTRHAAGVGMPCIVAACAL
DAGTQMFSPEGTAKIYGETFGQIDEFAHPLQAIAKAV

Sequences:

>Translated_437_residues
MAKYKDVIDLYDDNGKLLKSNVALEKISPLVNPAIKKIIDDTKRTVAVNLGGMQDALKTGKIGKHQQILGRELNLDIVGN
IDAIEAKIKEYVSVEAGDDTMIKRYNGGKLLLVKVPSARIAAAATYDAALTSVAAATTYAVVEQFNVDMFDANTVKAAAF
GTYPVTMDLAGGACSMIMSIPQNNESLGYALRNIPANQSVMITHRNAMQGAALTSVFEQAGEFEMGSAIGPFERAQLLLL
AYQGLNANNIVYDLVKENGQTGTVGTVVQSLVERAIEDKVITQGAAAKGGYFKPYETKDPMLWNAYTSAGTLAATMVNCG
AGRFAQAVSSTLLYFNDLIEHETGLPGSDFGRTMGVAVGFSFFSHSIYGGGGPGIFNGNHVVTRHAAGVGMPCIVAACAL
DAGTQMFSPEGTAKIYGETFGQIDEFAHPLQAIAKAV
>Mature_436_residues
AKYKDVIDLYDDNGKLLKSNVALEKISPLVNPAIKKIIDDTKRTVAVNLGGMQDALKTGKIGKHQQILGRELNLDIVGNI
DAIEAKIKEYVSVEAGDDTMIKRYNGGKLLLVKVPSARIAAAATYDAALTSVAAATTYAVVEQFNVDMFDANTVKAAAFG
TYPVTMDLAGGACSMIMSIPQNNESLGYALRNIPANQSVMITHRNAMQGAALTSVFEQAGEFEMGSAIGPFERAQLLLLA
YQGLNANNIVYDLVKENGQTGTVGTVVQSLVERAIEDKVITQGAAAKGGYFKPYETKDPMLWNAYTSAGTLAATMVNCGA
GRFAQAVSSTLLYFNDLIEHETGLPGSDFGRTMGVAVGFSFFSHSIYGGGGPGIFNGNHVVTRHAAGVGMPCIVAACALD
AGTQMFSPEGTAKIYGETFGQIDEFAHPLQAIAKAV

Specific function: Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and an heterodisulfide [H]

COG id: COG4054

COG function: function code H; Methyl coenzyme M reductase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022681
- InterPro:   IPR008924
- InterPro:   IPR015823
- InterPro:   IPR003179
- InterPro:   IPR022679
- InterPro:   IPR022680
- InterPro:   IPR009024 [H]

Pfam domain/function: PF02241 MCR_beta; PF02783 MCR_beta_N [H]

EC number: =2.8.4.1 [H]

Molecular weight: Translated: 46264; Mature: 46133

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKYKDVIDLYDDNGKLLKSNVALEKISPLVNPAIKKIIDDTKRTVAVNLGGMQDALKTG
CCCCCCCCEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHC
KIGKHQQILGRELNLDIVGNIDAIEAKIKEYVSVEAGDDTMIKRYNGGKLLLVKVPSARI
CCCCHHHHHCCCCCEEEECCCHHHHHHHHHHEEEECCCCCEEEEECCCEEEEEECCCCCE
AAAATYDAALTSVAAATTYAVVEQFNVDMFDANTVKAAAFGTYPVTMDLAGGACSMIMSI
EHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEEEECCCEEEEECCCCHHHHHEEC
PQNNESLGYALRNIPANQSVMITHRNAMQGAALTSVFEQAGEFEMGSAIGPFERAQLLLL
CCCCCHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHEEEEE
AYQGLNANNIVYDLVKENGQTGTVGTVVQSLVERAIEDKVITQGAAAKGGYFKPYETKDP
EECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
MLWNAYTSAGTLAATMVNCGAGRFAQAVSSTLLYFNDLIEHETGLPGSDFGRTMGVAVGF
EEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
SFFSHSIYGGGGPGIFNGNHVVTRHAAGVGMPCIVAACALDAGTQMFSPEGTAKIYGETF
HHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEECCCCCEEEHHHHH
GQIDEFAHPLQAIAKAV
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKYKDVIDLYDDNGKLLKSNVALEKISPLVNPAIKKIIDDTKRTVAVNLGGMQDALKTG
CCCCCCCEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHC
KIGKHQQILGRELNLDIVGNIDAIEAKIKEYVSVEAGDDTMIKRYNGGKLLLVKVPSARI
CCCCHHHHHCCCCCEEEECCCHHHHHHHHHHEEEECCCCCEEEEECCCEEEEEECCCCCE
AAAATYDAALTSVAAATTYAVVEQFNVDMFDANTVKAAAFGTYPVTMDLAGGACSMIMSI
EHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEEEECCCEEEEECCCCHHHHHEEC
PQNNESLGYALRNIPANQSVMITHRNAMQGAALTSVFEQAGEFEMGSAIGPFERAQLLLL
CCCCCHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHEEEEE
AYQGLNANNIVYDLVKENGQTGTVGTVVQSLVERAIEDKVITQGAAAKGGYFKPYETKDP
EECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
MLWNAYTSAGTLAATMVNCGAGRFAQAVSSTLLYFNDLIEHETGLPGSDFGRTMGVAVGF
EEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
SFFSHSIYGGGGPGIFNGNHVVTRHAAGVGMPCIVAACALDAGTQMFSPEGTAKIYGETF
HHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEECCCCCEEEHHHHH
GQIDEFAHPLQAIAKAV
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3502709; 11023796 [H]