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The map label for this gene is purN [H]

Identifier: 124486301

GI number: 124486301

Start: 1520699

End: 1521319

Strand: Reverse

Name: purN [H]

Synonym: Mlab_1486

Alternate gene names: 124486301

Gene position: 1521319-1520699 (Counterclockwise)

Preceding gene: 124486302

Following gene: 124486300

Centisome position: 84.29

GC content: 49.6

Gene sequence:

>621_bases
ATGAAGCGTATCGCGGTACTTGCATCCGGACGCGGGTCCAACTTCCAGGCGATTCTGGATGCTCTTGCAGCAGGCAAAAT
CAACGGAGAGATTGTGGCTCTTTTGACGGACAACAGAGATGCCTATGCAATCGAACGTGCAGATGCTGCCGGGATTCCTG
CTATTGTTCTCAATTACAAAGATTATCCCTCCAAAGAGGCGTATGAACGCGATCTTCTTACTGCTATGCAGGATATTTGT
GCCGATCTTTTTGTTTGTGCCGGATATATGCGTATCATCGGTTCTAAGATTGCCCGGGAATTTTCCGGAAAAATGATAAA
TATCCATCCGGCCCTTCTCCCTGCGTTTTCCGGTCTCCACGGGCAGCGTCAGGCTCTGGAATACGGGGTAAAAATCGCCG
GCTGCACAGTTCATTTCGTTGACGAAGGTCTCGATTCCGGCCCGATCATTTTACAGAAATCTGTCGAGGTTCTGGATGAC
GATGATGAAGACTCTTTGTCGGAGAGGATCTTAGAACAGGAACACCGGGCATTTCCCGAAGCCGTAGCATTGTTCTGTGC
CGATCGCCTGACGGTTGTCGGCCGTCATGTGAAAATACTTCCCGAGGCAAACAATCAATAG

Upstream 100 bases:

>100_bases
AATATCCTACGTTCTCGTGCTGCTCATCATTATCCCGCTGATCATGCCGTATCTCCTGTAAGGGAAGGTTAATTATCTCT
CTACTTCCCACATAACTATC

Downstream 100 bases:

>100_bases
GTCTGAACCCTTATTGTATAAGGCATAATCTCTATGGCAAAAGCAGAAAAAGGCGTCTCAGCAAAAAAAATCGCTCAGGA
ACGGGTCGATATTTTGTTTG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MKRIAVLASGRGSNFQAILDALAAGKINGEIVALLTDNRDAYAIERADAAGIPAIVLNYKDYPSKEAYERDLLTAMQDIC
ADLFVCAGYMRIIGSKIAREFSGKMINIHPALLPAFSGLHGQRQALEYGVKIAGCTVHFVDEGLDSGPIILQKSVEVLDD
DDEDSLSERILEQEHRAFPEAVALFCADRLTVVGRHVKILPEANNQ

Sequences:

>Translated_206_residues
MKRIAVLASGRGSNFQAILDALAAGKINGEIVALLTDNRDAYAIERADAAGIPAIVLNYKDYPSKEAYERDLLTAMQDIC
ADLFVCAGYMRIIGSKIAREFSGKMINIHPALLPAFSGLHGQRQALEYGVKIAGCTVHFVDEGLDSGPIILQKSVEVLDD
DDEDSLSERILEQEHRAFPEAVALFCADRLTVVGRHVKILPEANNQ
>Mature_206_residues
MKRIAVLASGRGSNFQAILDALAAGKINGEIVALLTDNRDAYAIERADAAGIPAIVLNYKDYPSKEAYERDLLTAMQDIC
ADLFVCAGYMRIIGSKIAREFSGKMINIHPALLPAFSGLHGQRQALEYGVKIAGCTVHFVDEGLDSGPIILQKSVEVLDD
DDEDSLSERILEQEHRAFPEAVALFCADRLTVVGRHVKILPEANNQ

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=182, Percent_Identity=40.1098901098901, Blast_Score=151, Evalue=3e-37,
Organism=Homo sapiens, GI209869995, Length=182, Percent_Identity=40.1098901098901, Blast_Score=151, Evalue=3e-37,
Organism=Homo sapiens, GI209869993, Length=182, Percent_Identity=40.1098901098901, Blast_Score=151, Evalue=3e-37,
Organism=Escherichia coli, GI1788846, Length=189, Percent_Identity=42.8571428571429, Blast_Score=165, Evalue=2e-42,
Organism=Escherichia coli, GI1787483, Length=200, Percent_Identity=29.5, Blast_Score=84, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=180, Percent_Identity=36.6666666666667, Blast_Score=129, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6320616, Length=205, Percent_Identity=30.7317073170732, Blast_Score=92, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24582400, Length=207, Percent_Identity=45.4106280193237, Blast_Score=192, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22455; Mature: 22455

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIAVLASGRGSNFQAILDALAAGKINGEIVALLTDNRDAYAIERADAAGIPAIVLNYK
CCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEEEECCCCCCCCEEEEECC
DYPSKEAYERDLLTAMQDICADLFVCAGYMRIIGSKIAREFSGKMINIHPALLPAFSGLH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHCCCC
GQRQALEYGVKIAGCTVHFVDEGLDSGPIILQKSVEVLDDDDEDSLSERILEQEHRAFPE
HHHHHHHHCCEEEEEEEEEECCCCCCCCEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHH
AVALFCADRLTVVGRHVKILPEANNQ
HHHHHHHHHHHHHCCEEEEECCCCCC
>Mature Secondary Structure
MKRIAVLASGRGSNFQAILDALAAGKINGEIVALLTDNRDAYAIERADAAGIPAIVLNYK
CCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEEEECCCCCCCCEEEEECC
DYPSKEAYERDLLTAMQDICADLFVCAGYMRIIGSKIAREFSGKMINIHPALLPAFSGLH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHCCCC
GQRQALEYGVKIAGCTVHFVDEGLDSGPIILQKSVEVLDDDDEDSLSERILEQEHRAFPE
HHHHHHHHCCEEEEEEEEEECCCCCCCCEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHH
AVALFCADRLTVVGRHVKILPEANNQ
HHHHHHHHHHHHHCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]