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The map label for this gene is trpE [H]

Identifier: 124486216

GI number: 124486216

Start: 1427880

End: 1429406

Strand: Reverse

Name: trpE [H]

Synonym: Mlab_1399

Alternate gene names: 124486216

Gene position: 1429406-1427880 (Counterclockwise)

Preceding gene: 124486218

Following gene: 124486215

Centisome position: 79.19

GC content: 49.44

Gene sequence:

>1527_bases
ATGGAAACTGGTATAATCGGGGACTCTGGTCAAAATGCGGTATTTCCCACAAAAGAGGAATTACCGGCGCTCAAAAAGAC
CTTAACGGCCAATACGCTGATCCCCGTTTATACGACTTTTACCGACTCGGGAATCTCGCCTGCATCTGCCTATGAGGCAC
TCAGAGGAGAATACGGTTTTCTCCTCGAATCGATAGAAGGAAATGAACATAATGCACGATACTCGGTCCTTGGGACCGGC
CTTCTGATGCATCTAACTGCAGATCCGGGAATCAGCATTTCCGGAAAATACATTCCCGCGAAGGGAATCGAAGGCCGGGA
TGTGTCTGCGGACTCGCTCAATGAACTATTACGCTGTATCGAGTATCAAAGTCCTGATATTCCCGGATACGCCGGAGGTC
TTGCCGGATACTTCTCGTATGATCTGGCCCTTCAAACCCTAAGCGTCCCCGGCATCAAAGAAAAACCGCGGGATTTCCCT
CTTGCCGAGTTCATGATGCCGGAAGAGTATGTTGTATTCGATCATGTGCGTGGAACGATCACCATTCTCAGATTTTTATT
CGGGATGGATTCAAAAGACATCGATTCCGCATACGCCGAGGCCCTTCGTCAGATCTCCGCAGCGAAAAAGACTCTCTGCG
GATTAGCATCATTTGAAGAGAAATTTCCGTGCGAAAAAATGGGTAAGGCCGAATCAACATATACATCCGGCATATCAAAA
AACGGGTTCGAAGATCTCGTGAGAACCGCAAAAGAGTATATTCTCAACGGGGATATCTTCCAGGTTGTCATCTCAAAACA
ATGCGCTGTCCCTTATGAAAGCGATCCCTTTAGGCTATATCGGCAGATGAGGAAACTGAACCCGAGTCCCTATCTGTATT
TCATGGACTTCTCCGACAAACAGGTCATCGGAGCAAGTCCCGAGATGCTGATCAAAGTGGAAGGGCGGCAGGTCACCTCG
GTTCCAATCGCCGGGACCAGAAAGCGCGGGAAGGATGCAAAAGAGGATATCGAACTGGAAAGGGAACTCTTAGCTGATAA
AAAAGAGTGCGCCGAGCATCTGATGCTCGTTGATCTTTCGAGAAATGATATCGGCCGCGTCTCCAAATACGGAAGCGTTC
GGGTGACTGACTTCATGTCGGTTGAAAAATTCTCCCATGTTCAGCACATCGTATCGACCGTTGAGGGCATCCTTTCGGAT
GATCAGACCTCCTTCGATGCCTTCATGTCGTGTTTTCCTGCTGGAACCGTCTCCGGGGCACCGAAAATTCGCGCTATGCA
GATCATAAATGAACTTGAACCGACATCCCGCGGCCCGTATGCCGGCGCCGTCGGCTATATCGGATTTAATGGAAATCTGG
ATCTTGCGATCCTTATCCGGACCGTCGTGGTAAAAGACAAAACTGCGTATTTCCAGTCCGGAGCCGGCATCGTTGCGGAT
TCCAATCCTGCATCTGAATTCATCGAGTCGGAAAACAAGGCCGCAAGCATGAAGGCAGCAATTGAATCGGCGGGTGAAAT
CGCATGA

Upstream 100 bases:

>100_bases
TTGAATTATTTGTACAGTAATGTATATATATGTACAACAGACAACAATTGTTGTGAATTATGCAAACAATCAATACGAAT
CGGATGGTGATGCCGGTGGA

Downstream 100 bases:

>100_bases
ACGTTCTGGTAATCGACTGTTACGACAGTTTCACCTACAATCTCTGTCAGCAGATAGGATATCTCGGGGGAAAACCGATC
GTGGTTAAAAATGACCGACC

Product: anthranilate synthase, component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
METGIIGDSGQNAVFPTKEELPALKKTLTANTLIPVYTTFTDSGISPASAYEALRGEYGFLLESIEGNEHNARYSVLGTG
LLMHLTADPGISISGKYIPAKGIEGRDVSADSLNELLRCIEYQSPDIPGYAGGLAGYFSYDLALQTLSVPGIKEKPRDFP
LAEFMMPEEYVVFDHVRGTITILRFLFGMDSKDIDSAYAEALRQISAAKKTLCGLASFEEKFPCEKMGKAESTYTSGISK
NGFEDLVRTAKEYILNGDIFQVVISKQCAVPYESDPFRLYRQMRKLNPSPYLYFMDFSDKQVIGASPEMLIKVEGRQVTS
VPIAGTRKRGKDAKEDIELERELLADKKECAEHLMLVDLSRNDIGRVSKYGSVRVTDFMSVEKFSHVQHIVSTVEGILSD
DQTSFDAFMSCFPAGTVSGAPKIRAMQIINELEPTSRGPYAGAVGYIGFNGNLDLAILIRTVVVKDKTAYFQSGAGIVAD
SNPASEFIESENKAASMKAAIESAGEIA

Sequences:

>Translated_508_residues
METGIIGDSGQNAVFPTKEELPALKKTLTANTLIPVYTTFTDSGISPASAYEALRGEYGFLLESIEGNEHNARYSVLGTG
LLMHLTADPGISISGKYIPAKGIEGRDVSADSLNELLRCIEYQSPDIPGYAGGLAGYFSYDLALQTLSVPGIKEKPRDFP
LAEFMMPEEYVVFDHVRGTITILRFLFGMDSKDIDSAYAEALRQISAAKKTLCGLASFEEKFPCEKMGKAESTYTSGISK
NGFEDLVRTAKEYILNGDIFQVVISKQCAVPYESDPFRLYRQMRKLNPSPYLYFMDFSDKQVIGASPEMLIKVEGRQVTS
VPIAGTRKRGKDAKEDIELERELLADKKECAEHLMLVDLSRNDIGRVSKYGSVRVTDFMSVEKFSHVQHIVSTVEGILSD
DQTSFDAFMSCFPAGTVSGAPKIRAMQIINELEPTSRGPYAGAVGYIGFNGNLDLAILIRTVVVKDKTAYFQSGAGIVAD
SNPASEFIESENKAASMKAAIESAGEIA
>Mature_508_residues
METGIIGDSGQNAVFPTKEELPALKKTLTANTLIPVYTTFTDSGISPASAYEALRGEYGFLLESIEGNEHNARYSVLGTG
LLMHLTADPGISISGKYIPAKGIEGRDVSADSLNELLRCIEYQSPDIPGYAGGLAGYFSYDLALQTLSVPGIKEKPRDFP
LAEFMMPEEYVVFDHVRGTITILRFLFGMDSKDIDSAYAEALRQISAAKKTLCGLASFEEKFPCEKMGKAESTYTSGISK
NGFEDLVRTAKEYILNGDIFQVVISKQCAVPYESDPFRLYRQMRKLNPSPYLYFMDFSDKQVIGASPEMLIKVEGRQVTS
VPIAGTRKRGKDAKEDIELERELLADKKECAEHLMLVDLSRNDIGRVSKYGSVRVTDFMSVEKFSHVQHIVSTVEGILSD
DQTSFDAFMSCFPAGTVSGAPKIRAMQIINELEPTSRGPYAGAVGYIGFNGNLDLAILIRTVVVKDKTAYFQSGAGIVAD
SNPASEFIESENKAASMKAAIESAGEIA

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=380, Percent_Identity=37.8947368421053, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1788114, Length=379, Percent_Identity=31.3984168865435, Blast_Score=212, Evalue=5e-56,
Organism=Escherichia coli, GI1786809, Length=261, Percent_Identity=27.5862068965517, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082077, Length=270, Percent_Identity=26.2962962962963, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320935, Length=488, Percent_Identity=38.1147540983607, Blast_Score=293, Evalue=6e-80,
Organism=Saccharomyces cerevisiae, GI6324361, Length=402, Percent_Identity=25.1243781094527, Blast_Score=108, Evalue=2e-24,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 55530; Mature: 55530

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METGIIGDSGQNAVFPTKEELPALKKTLTANTLIPVYTTFTDSGISPASAYEALRGEYGF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHCCCHHH
LLESIEGNEHNARYSVLGTGLLMHLTADPGISISGKYIPAKGIEGRDVSADSLNELLRCI
HHHHCCCCCCCCEEEEEECCEEEEEECCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHH
EYQSPDIPGYAGGLAGYFSYDLALQTLSVPGIKEKPRDFPLAEFMMPEEYVVFDHVRGTI
HCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHCCCCCEEEEHHHHHHH
TILRFLFGMDSKDIDSAYAEALRQISAAKKTLCGLASFEEKFPCEKMGKAESTYTSGISK
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHCCCCC
NGFEDLVRTAKEYILNGDIFQVVISKQCAVPYESDPFRLYRQMRKLNPSPYLYFMDFSDK
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCC
QVIGASPEMLIKVEGRQVTSVPIAGTRKRGKDAKEDIELERELLADKKECAEHLMLVDLS
EEECCCCCEEEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHEEEECC
RNDIGRVSKYGSVRVTDFMSVEKFSHVQHIVSTVEGILSDDQTSFDAFMSCFPAGTVSGA
CCCHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCC
PKIRAMQIINELEPTSRGPYAGAVGYIGFNGNLDLAILIRTVVVKDKTAYFQSGAGIVAD
CHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCHHHHHCCCCEEEC
SNPASEFIESENKAASMKAAIESAGEIA
CCHHHHHHHCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
METGIIGDSGQNAVFPTKEELPALKKTLTANTLIPVYTTFTDSGISPASAYEALRGEYGF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHCCCHHH
LLESIEGNEHNARYSVLGTGLLMHLTADPGISISGKYIPAKGIEGRDVSADSLNELLRCI
HHHHCCCCCCCCEEEEEECCEEEEEECCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHH
EYQSPDIPGYAGGLAGYFSYDLALQTLSVPGIKEKPRDFPLAEFMMPEEYVVFDHVRGTI
HCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHCCCCCEEEEHHHHHHH
TILRFLFGMDSKDIDSAYAEALRQISAAKKTLCGLASFEEKFPCEKMGKAESTYTSGISK
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHCCCCC
NGFEDLVRTAKEYILNGDIFQVVISKQCAVPYESDPFRLYRQMRKLNPSPYLYFMDFSDK
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCC
QVIGASPEMLIKVEGRQVTSVPIAGTRKRGKDAKEDIELERELLADKKECAEHLMLVDLS
EEECCCCCEEEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHEEEECC
RNDIGRVSKYGSVRVTDFMSVEKFSHVQHIVSTVEGILSDDQTSFDAFMSCFPAGTVSGA
CCCHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCC
PKIRAMQIINELEPTSRGPYAGAVGYIGFNGNLDLAILIRTVVVKDKTAYFQSGAGIVAD
CHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCHHHHHCCCCEEEC
SNPASEFIESENKAASMKAAIESAGEIA
CCHHHHHHHCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2732211 [H]