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The map label for this gene is priA

Identifier: 124486122

GI number: 124486122

Start: 1327586

End: 1328728

Strand: Reverse

Name: priA

Synonym: Mlab_1303

Alternate gene names: 124486122

Gene position: 1328728-1327586 (Counterclockwise)

Preceding gene: 124486123

Following gene: 124486121

Centisome position: 73.62

GC content: 53.98

Gene sequence:

>1143_bases
ATGAAACCTGCCACTCTGGAATTTCTCAGGCAGCGCTTTTTTGCCTACTACAATGGGGCTATCCCGGGAGCGGGTGCGGG
ATATAGCCCCGAATCCCTTACTGAACGGGAGTGGGGGTTTCTTTTCTTTACGGAAAACCAGCGTTCCGGGATGCGGCGTC
ATATCTCGTTCACTTCTCAGGATGAGCTGACCTCCTATCTGAAAAGCATGGTTCCGGCTCATGTGTATTACTCGACCGCA
TATTACACCCATCCGGGGGCAGCCCAGATGGCGGATAAAGAGTGGCGCGGGGCTGATGTCATCTTCGATTTAGATGCGGA
TCATATCGTTCGCGGCCCGTATGATATCATGCTTGCCCGTGTCAAAGAGGAACTTTTCAAACTCATCGATATGCTTACTG
GAGAGCTCGGGTTTGCAAAACGGGATCTTCGAATCAACTTTTCCGGCGGGCGCGGATATCATGTCCATCTCCCGCTTCTT
TCCGTTCGGGGCTGGGATACCGCCGAGCGGCGCGAACTGGTGAATTACGTCTCGGGCACCGGTCTTTCATTCGACAGCAT
GATGGCCCCCTCCCAAAAATCCGGCTGGAAGCTTCGCTATCACGATGCTCTAAACGATGAACTCGCCCGGATCGCGGGAC
TGCCGTGTGAAGATGCGCTTTCCTATTTGTCAGGCTTGTCCGGGATATCGGAGACGTTTGCGGCAGGATTTTTGAAAAAC
ATCTCTCAGACCCAAAATCTGCTCAAATCGAATCCGGAAAAGCTTCTTGCAAACAAAGTGGTGCGGGCGATCGCCAACGC
GGAAAATGAACCGTTCCAGGCAGGAATCCTCTCCCGCGCAGCCCAGGCGGACGAGCCGGTAACGACCGATGTGAAGCGTC
TGATCCGTCACCCCGGCTCTCTGCACGGAGGATCCGGCATGCGCGTCGTTCCGATTTCCGTTGATCAGCTGGACGCATTC
GATCCTTTAATCGATGCCGTGGTGTTTGGCGAACGAAATGTCTCCGTTGACTGCGCCTTCAATCTCTCCATGCCTATCCT
TGGGAATAATTATACGCTGACAGCGGGCCGAAATGTTGTTCCCGAGGCATTGGGCGTTTTTCTCTGCTGCAGAGGGATTG
CCGAATTGTCGGGAGGTATCTGA

Upstream 100 bases:

>100_bases
CCGGATATTCCACGGTGGCCGTGATGAGCGGCATTGGGGTAAGACCCTATTATCATAGACAGGGATATCAGCGTATAGGT
CCATATATGATTAAGAATCT

Downstream 100 bases:

>100_bases
ATGAGCCGCATCAGTACCTCCGATCTGCACGGATACTTGATCGACGAGAGAAATATGGGTTCCCTTGCCGAAATTCCGGC
AACGCTCTATGAAGAGATCC

Product: DNA primase small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MKPATLEFLRQRFFAYYNGAIPGAGAGYSPESLTEREWGFLFFTENQRSGMRRHISFTSQDELTSYLKSMVPAHVYYSTA
YYTHPGAAQMADKEWRGADVIFDLDADHIVRGPYDIMLARVKEELFKLIDMLTGELGFAKRDLRINFSGGRGYHVHLPLL
SVRGWDTAERRELVNYVSGTGLSFDSMMAPSQKSGWKLRYHDALNDELARIAGLPCEDALSYLSGLSGISETFAAGFLKN
ISQTQNLLKSNPEKLLANKVVRAIANAENEPFQAGILSRAAQADEPVTTDVKRLIRHPGSLHGGSGMRVVPISVDQLDAF
DPLIDAVVFGERNVSVDCAFNLSMPILGNNYTLTAGRNVVPEALGVFLCCRGIAELSGGI

Sequences:

>Translated_380_residues
MKPATLEFLRQRFFAYYNGAIPGAGAGYSPESLTEREWGFLFFTENQRSGMRRHISFTSQDELTSYLKSMVPAHVYYSTA
YYTHPGAAQMADKEWRGADVIFDLDADHIVRGPYDIMLARVKEELFKLIDMLTGELGFAKRDLRINFSGGRGYHVHLPLL
SVRGWDTAERRELVNYVSGTGLSFDSMMAPSQKSGWKLRYHDALNDELARIAGLPCEDALSYLSGLSGISETFAAGFLKN
ISQTQNLLKSNPEKLLANKVVRAIANAENEPFQAGILSRAAQADEPVTTDVKRLIRHPGSLHGGSGMRVVPISVDQLDAF
DPLIDAVVFGERNVSVDCAFNLSMPILGNNYTLTAGRNVVPEALGVFLCCRGIAELSGGI
>Mature_380_residues
MKPATLEFLRQRFFAYYNGAIPGAGAGYSPESLTEREWGFLFFTENQRSGMRRHISFTSQDELTSYLKSMVPAHVYYSTA
YYTHPGAAQMADKEWRGADVIFDLDADHIVRGPYDIMLARVKEELFKLIDMLTGELGFAKRDLRINFSGGRGYHVHLPLL
SVRGWDTAERRELVNYVSGTGLSFDSMMAPSQKSGWKLRYHDALNDELARIAGLPCEDALSYLSGLSGISETFAAGFLKN
ISQTQNLLKSNPEKLLANKVVRAIANAENEPFQAGILSRAAQADEPVTTDVKRLIRHPGSLHGGSGMRVVPISVDQLDAF
DPLIDAVVFGERNVSVDCAFNLSMPILGNNYTLTAGRNVVPEALGVFLCCRGIAELSGGI

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis

COG id: COG1467

COG function: function code L; Eukaryotic-type DNA primase, catalytic (small) subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic-type primase small subunit family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIS_METLZ (A2ST15)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001030738.1
- ProteinModelPortal:   A2ST15
- SMR:   A2ST15
- STRING:   A2ST15
- GeneID:   4794547
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_1303
- eggNOG:   arNOG05167
- HOGENOM:   HBG512871
- OMA:   SGGRGYH
- ProtClustDB:   CLSK2516869
- BioCyc:   MLAB410358:MLAB_1303-MONOMER
- HAMAP:   MF_00700
- InterPro:   IPR002755
- InterPro:   IPR014052
- TIGRFAMs:   TIGR00335

Pfam domain/function: PF01896 DNA_primase_S

EC number: 2.7.7.-

Molecular weight: Translated: 41900; Mature: 41900

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: ACT_SITE 103-103 ACT_SITE 105-105

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPATLEFLRQRFFAYYNGAIPGAGAGYSPESLTEREWGFLFFTENQRSGMRRHISFTSQ
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHCCCCCH
DELTSYLKSMVPAHVYYSTAYYTHPGAAQMADKEWRGADVIFDLDADHIVRGPYDIMLAR
HHHHHHHHHHCCHHHEEEEEEEECCCHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHH
VKEELFKLIDMLTGELGFAKRDLRINFSGGRGYHVHLPLLSVRGWDTAERRELVNYVSGT
HHHHHHHHHHHHHCCCCCEEEEEEEEECCCCEEEEEEEEEEECCCCHHHHHHHHHHHHCC
GLSFDSMMAPSQKSGWKLRYHDALNDELARIAGLPCEDALSYLSGLSGISETFAAGFLKN
CCCHHHHCCCCCCCCCEEEECHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHH
ISQTQNLLKSNPEKLLANKVVRAIANAENEPFQAGILSRAAQADEPVTTDVKRLIRHPGS
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
LHGGSGMRVVPISVDQLDAFDPLIDAVVFGERNVSVDCAFNLSMPILGNNYTLTAGRNVV
CCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEECCCEEEECCCCCC
PEALGVFLCCRGIAELSGGI
HHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKPATLEFLRQRFFAYYNGAIPGAGAGYSPESLTEREWGFLFFTENQRSGMRRHISFTSQ
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHCCCCCH
DELTSYLKSMVPAHVYYSTAYYTHPGAAQMADKEWRGADVIFDLDADHIVRGPYDIMLAR
HHHHHHHHHHCCHHHEEEEEEEECCCHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHH
VKEELFKLIDMLTGELGFAKRDLRINFSGGRGYHVHLPLLSVRGWDTAERRELVNYVSGT
HHHHHHHHHHHHHCCCCCEEEEEEEEECCCCEEEEEEEEEEECCCCHHHHHHHHHHHHCC
GLSFDSMMAPSQKSGWKLRYHDALNDELARIAGLPCEDALSYLSGLSGISETFAAGFLKN
CCCHHHHCCCCCCCCCEEEECHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHH
ISQTQNLLKSNPEKLLANKVVRAIANAENEPFQAGILSRAAQADEPVTTDVKRLIRHPGS
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
LHGGSGMRVVPISVDQLDAFDPLIDAVVFGERNVSVDCAFNLSMPILGNNYTLTAGRNVV
CCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEECCCEEEECCCCCC
PEALGVFLCCRGIAELSGGI
HHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA