Click here to switch to the map view.

The map label for this gene is glnA [H]

Identifier: 124486080

GI number: 124486080

Start: 1288329

End: 1289714

Strand: Reverse

Name: glnA [H]

Synonym: Mlab_1260

Alternate gene names: 124486080

Gene position: 1289714-1288329 (Counterclockwise)

Preceding gene: 124486087

Following gene: 124486079

Centisome position: 71.45

GC content: 54.11

Gene sequence:

>1386_bases
ATGGTTTCTGACGAGCGTAAAAGTCCTTTTGGCTCTCTTGCCGCTTCACACGGCGGCGACCGTGCGGAGTTGATTGACGC
AGTTCTCAAACAGCTTGAGAATGACAATGTACGGTCGGTTCTCCTCCAGTTCTCGGACATGGTGGGCAAACCGAAAAATG
TCGCGATTCCGACGAAACAGATGAAGAAAGCCTTAACTGAGGGTATCAGTTTCGACGGTTCTTCAATTCAGGGGTTTGCG
AGACTGGAAGAGTCGGATATGGTGCTCCGGCCCGAGCCGGCAACATATCAGATCATTCCCTGGTCCGATGAGAAATACCG
GGTTGCCCGATTCATCTGCGATGTTTATACGACCCGCGGCGAACCGTTCATCGGGGACCCGCGGTATATTCTCCGTCAGC
AGCTGGAAAAAGCGGCAAAACTCGGATACACCTTCAATGTCGGCCCCGAGATGGAGTTTTTCCTCTTCAGACTGATCAAT
GGCCACCCCTCGGTGGAACTTCAGGATCACGGAGGATACTTCGATCAGACCCCGACCGACCCCGGAGAGGATGTCCGCCG
CGATTTAGTGACCTCCCTTTCCGAGATGGGATTTGATATCGAAGCATCCCATCACGAGGTCGCACCCTCCCAGCACGAGA
TCGATTTCACCTACGGCAACGCCCTTGCCATGGCAGATAAGGTTGTTACCTTCAAGTTTGTTGCAAAAACGCTCGCTCTT
CACCGCGGTCTGCATGCCACTTTCATGGCAAAACCGATCTACGGCATCAACGGTTCCGGGATGCATGTCAACTGTTCGCT
GATGAAGGACGGACAGAATGCATTCTTTGATCCCGAAGGAGAACACCAGCTCTCCGACACCGCCCGTCATTTTATTGCCG
GAATTCTCAAACATATCGATGGAATCACCCGTATCGCAAACCCGACGGTGAACTCGTACAAGCGGCTTATCCCGGGATAC
GAAGCTCCGGTCTATGTCGGCTGGTCTGCGATGAACCGTTCCGCCCTTATCAGAGTTCCCTCGTCCCGCGGCAAAAGTAC
CCGCGCCGAACTGCGCAGCCCGGACCCGACCTGCAACCCGTATCTCACGTTTGCCGTGATGCTTGCAGCCGGTATGGAAG
GCGTTACCCAGAAGATTGAGCCCCCGGAGAGCGTGGACAAAAATATTTTCAGAATGAGCGCGGAAGAGCGTGCAGCTGAA
GGAATACGCTGCCTCCCCTGGAGTCTCCATGAGGCAAACACCGCTCTGATGAACGATCCGCTGCTCTGCAGTGTTCTCGG
CGAGCATGTGGTTTCGCAGATCAACCGTCTCGGTGAACTCGAGTGGGGAGATTTCTCCAAGCATATCACTGACTGGGAGC
TTAACCGCTACCTTGCAACATACTAA

Upstream 100 bases:

>100_bases
TCTGGTATGTCAAGTTTTCCAGAAAATCCCCTGCTGAAAAATGGTCATGCGCCCGGATTCGAACTTGACATTTATTAGCC
TTCACCACTCATATATGAGC

Downstream 100 bases:

>100_bases
ATCATACCAACTCTTTTTTTCGATCCGCCGTTTTTTTTTGAATTCGCCGATGTACCGCGGCGCTTTTTATCCATCTTTGA
TGCGCTGACAGCAAATGTAT

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MVSDERKSPFGSLAASHGGDRAELIDAVLKQLENDNVRSVLLQFSDMVGKPKNVAIPTKQMKKALTEGISFDGSSIQGFA
RLEESDMVLRPEPATYQIIPWSDEKYRVARFICDVYTTRGEPFIGDPRYILRQQLEKAAKLGYTFNVGPEMEFFLFRLIN
GHPSVELQDHGGYFDQTPTDPGEDVRRDLVTSLSEMGFDIEASHHEVAPSQHEIDFTYGNALAMADKVVTFKFVAKTLAL
HRGLHATFMAKPIYGINGSGMHVNCSLMKDGQNAFFDPEGEHQLSDTARHFIAGILKHIDGITRIANPTVNSYKRLIPGY
EAPVYVGWSAMNRSALIRVPSSRGKSTRAELRSPDPTCNPYLTFAVMLAAGMEGVTQKIEPPESVDKNIFRMSAEERAAE
GIRCLPWSLHEANTALMNDPLLCSVLGEHVVSQINRLGELEWGDFSKHITDWELNRYLATY

Sequences:

>Translated_461_residues
MVSDERKSPFGSLAASHGGDRAELIDAVLKQLENDNVRSVLLQFSDMVGKPKNVAIPTKQMKKALTEGISFDGSSIQGFA
RLEESDMVLRPEPATYQIIPWSDEKYRVARFICDVYTTRGEPFIGDPRYILRQQLEKAAKLGYTFNVGPEMEFFLFRLIN
GHPSVELQDHGGYFDQTPTDPGEDVRRDLVTSLSEMGFDIEASHHEVAPSQHEIDFTYGNALAMADKVVTFKFVAKTLAL
HRGLHATFMAKPIYGINGSGMHVNCSLMKDGQNAFFDPEGEHQLSDTARHFIAGILKHIDGITRIANPTVNSYKRLIPGY
EAPVYVGWSAMNRSALIRVPSSRGKSTRAELRSPDPTCNPYLTFAVMLAAGMEGVTQKIEPPESVDKNIFRMSAEERAAE
GIRCLPWSLHEANTALMNDPLLCSVLGEHVVSQINRLGELEWGDFSKHITDWELNRYLATY
>Mature_461_residues
MVSDERKSPFGSLAASHGGDRAELIDAVLKQLENDNVRSVLLQFSDMVGKPKNVAIPTKQMKKALTEGISFDGSSIQGFA
RLEESDMVLRPEPATYQIIPWSDEKYRVARFICDVYTTRGEPFIGDPRYILRQQLEKAAKLGYTFNVGPEMEFFLFRLIN
GHPSVELQDHGGYFDQTPTDPGEDVRRDLVTSLSEMGFDIEASHHEVAPSQHEIDFTYGNALAMADKVVTFKFVAKTLAL
HRGLHATFMAKPIYGINGSGMHVNCSLMKDGQNAFFDPEGEHQLSDTARHFIAGILKHIDGITRIANPTVNSYKRLIPGY
EAPVYVGWSAMNRSALIRVPSSRGKSTRAELRSPDPTCNPYLTFAVMLAAGMEGVTQKIEPPESVDKNIFRMSAEERAAE
GIRCLPWSLHEANTALMNDPLLCSVLGEHVVSQINRLGELEWGDFSKHITDWELNRYLATY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=433, Percent_Identity=25.635103926097, Blast_Score=131, Evalue=1e-30,
Organism=Escherichia coli, GI1790301, Length=424, Percent_Identity=39.3867924528302, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI87081870, Length=385, Percent_Identity=26.4935064935065, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 51475; Mature: 51475

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSDERKSPFGSLAASHGGDRAELIDAVLKQLENDNVRSVLLQFSDMVGKPKNVAIPTKQ
CCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHH
MKKALTEGISFDGSSIQGFARLEESDMVLRPEPATYQIIPWSDEKYRVARFICDVYTTRG
HHHHHHHCCCCCCCCCCHHHHCCCCCCEECCCCCEEEEEECCCCHHHHHHHHHHHHHCCC
EPFIGDPRYILRQQLEKAAKLGYTFNVGPEMEFFLFRLINGHPSVELQDHGGYFDQTPTD
CCCCCCCHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
PGEDVRRDLVTSLSEMGFDIEASHHEVAPSQHEIDFTYGNALAMADKVVTFKFVAKTLAL
CHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
HRGLHATFMAKPIYGINGSGMHVNCSLMKDGQNAFFDPEGEHQLSDTARHFIAGILKHID
HCCCHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GITRIANPTVNSYKRLIPGYEAPVYVGWSAMNRSALIRVPSSRGKSTRAELRSPDPTCNP
HHHHHCCCCHHHHHHHCCCCCCCEEEEHHHCCCCEEEECCCCCCCCCHHHCCCCCCCCCH
YLTFAVMLAAGMEGVTQKIEPPESVDKNIFRMSAEERAAEGIRCLPWSLHEANTALMNDP
HHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHCHHHHHHCCCEECCCCHHHCCHHHHCCC
LLCSVLGEHVVSQINRLGELEWGDFSKHITDWELNRYLATY
HHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MVSDERKSPFGSLAASHGGDRAELIDAVLKQLENDNVRSVLLQFSDMVGKPKNVAIPTKQ
CCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHH
MKKALTEGISFDGSSIQGFARLEESDMVLRPEPATYQIIPWSDEKYRVARFICDVYTTRG
HHHHHHHCCCCCCCCCCHHHHCCCCCCEECCCCCEEEEEECCCCHHHHHHHHHHHHHCCC
EPFIGDPRYILRQQLEKAAKLGYTFNVGPEMEFFLFRLINGHPSVELQDHGGYFDQTPTD
CCCCCCCHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
PGEDVRRDLVTSLSEMGFDIEASHHEVAPSQHEIDFTYGNALAMADKVVTFKFVAKTLAL
CHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
HRGLHATFMAKPIYGINGSGMHVNCSLMKDGQNAFFDPEGEHQLSDTARHFIAGILKHID
HCCCHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GITRIANPTVNSYKRLIPGYEAPVYVGWSAMNRSALIRVPSSRGKSTRAELRSPDPTCNP
HHHHHCCCCHHHHHHHCCCCCCCEEEEHHHCCCCEEEECCCCCCCCCHHHCCCCCCCCCH
YLTFAVMLAAGMEGVTQKIEPPESVDKNIFRMSAEERAAEGIRCLPWSLHEANTALMNDP
HHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHCHHHHHHCCCEECCCCHHHCCHHHHCCC
LLCSVLGEHVVSQINRLGELEWGDFSKHITDWELNRYLATY
HHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2572584 [H]