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The map label for this gene is pcn

Identifier: 124486073

GI number: 124486073

Start: 1279408

End: 1280151

Strand: Reverse

Name: pcn

Synonym: Mlab_1253

Alternate gene names: 124486073

Gene position: 1280151-1279408 (Counterclockwise)

Preceding gene: 124486077

Following gene: 124486072

Centisome position: 70.92

GC content: 50.13

Gene sequence:

>744_bases
ATGTTAAAAGCAACCATTGAAGCAGATATTTTCCGTGAGACCATCGATGCGGTTTCAGCACTGGTAAACGAATGCCGTCT
TCACGTTGACGAGAAGGGAATCAGAACGATCACTGTGGATACTTCCAACGTGGCAATGGTTTCCTTAGAACTCTCTGCAT
CGGCGTTTCTCACATTCGCCGCAGAACCCTGTGAAATAGGTCTCGATATTGAAAAGATCAGATCGATGATGTCGATGATC
GGCAAGACGGACATCGTCTCTCTCGAACTTGACGAATCGGGCAAAAAACTCAAAATCAGTTTCGGCGGGTATGAATATTC
GATAACCCTGCTTGATACAAAGACCATCAGAAAAGACCCGAACGCGCCGAACCTGAATCTGCCCGCAACCTTTGAAGTTC
CCGGCGTAATGTTCAATGATGCCATCAAGGCTTCCTCAATGGTTTCCGATAAGATCTCCCTTGCCGTGTCTGCGGAGACC
TGCGTTTTTACCATGAATGCCGACGGTGATTCCGACCGGATCAAACGCGAACTTACCGGCGATGATGTGCATTACCTCAC
CTGTGCGGATGCGAGATCCCTTTTCTCTCTTGACTATCTCAAAGACATGGGCAAATCGATCGGCCGTGCCGAGAAGGTCC
AGATCCGTCTGGGCACCGATCACCCGGTCCAGTTCTCCTTCGTCTATGCAGGCGGCAAGGGAAGTGTGGGCTACCTTCTT
GCGCCGAGGATCGAGGCAGATTAA

Upstream 100 bases:

>100_bases
TTTCTCCCAACTTAACGTATATCTTATATGAGCGCGGATATAATTATACTATTAAAGTTTGTCACGCTAATATAAGAGAG
TGTGTCAAGGGGTTATATTG

Downstream 100 bases:

>100_bases
CCGTTATGCGCACGTCCGTTGAAAAGCGCGACCTGGCTCATTATCCTTTTTTACCTGAAGCACAGGGGCTGCTTGCATCC
CGCGGAATCAAAGTTGCCGG

Product: DNA polymerase sliding clamp

Products: NA

Alternate protein names: Proliferating cell nuclear antigen homolog; PCNA

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MLKATIEADIFRETIDAVSALVNECRLHVDEKGIRTITVDTSNVAMVSLELSASAFLTFAAEPCEIGLDIEKIRSMMSMI
GKTDIVSLELDESGKKLKISFGGYEYSITLLDTKTIRKDPNAPNLNLPATFEVPGVMFNDAIKASSMVSDKISLAVSAET
CVFTMNADGDSDRIKRELTGDDVHYLTCADARSLFSLDYLKDMGKSIGRAEKVQIRLGTDHPVQFSFVYAGGKGSVGYLL
APRIEAD

Sequences:

>Translated_247_residues
MLKATIEADIFRETIDAVSALVNECRLHVDEKGIRTITVDTSNVAMVSLELSASAFLTFAAEPCEIGLDIEKIRSMMSMI
GKTDIVSLELDESGKKLKISFGGYEYSITLLDTKTIRKDPNAPNLNLPATFEVPGVMFNDAIKASSMVSDKISLAVSAET
CVFTMNADGDSDRIKRELTGDDVHYLTCADARSLFSLDYLKDMGKSIGRAEKVQIRLGTDHPVQFSFVYAGGKGSVGYLL
APRIEAD
>Mature_247_residues
MLKATIEADIFRETIDAVSALVNECRLHVDEKGIRTITVDTSNVAMVSLELSASAFLTFAAEPCEIGLDIEKIRSMMSMI
GKTDIVSLELDESGKKLKISFGGYEYSITLLDTKTIRKDPNAPNLNLPATFEVPGVMFNDAIKASSMVSDKISLAVSAET
CVFTMNADGDSDRIKRELTGDDVHYLTCADARSLFSLDYLKDMGKSIGRAEKVQIRLGTDHPVQFSFVYAGGKGSVGYLL
APRIEAD

Specific function: Sliding clamp subunit. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PCNA family

Homologues:

Organism=Saccharomyces cerevisiae, GI6319564, Length=258, Percent_Identity=26.7441860465116, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PCNA_METLZ (A2SSW6)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001030689.1
- ProteinModelPortal:   A2SSW6
- STRING:   A2SSW6
- GeneID:   4794814
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_1253
- eggNOG:   arNOG05779
- HOGENOM:   HBG553564
- OMA:   DENGMSV
- PhylomeDB:   A2SSW6
- ProtClustDB:   PRK01115
- BioCyc:   MLAB410358:MLAB_1253-MONOMER
- HAMAP:   MF_00317
- InterPro:   IPR000730
- InterPro:   IPR022649
- InterPro:   IPR022648
- PANTHER:   PTHR11352
- PRINTS:   PR00339
- TIGRFAMs:   TIGR00590

Pfam domain/function: PF02747 PCNA_C; PF00705 PCNA_N

EC number: NA

Molecular weight: Translated: 26908; Mature: 26908

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS01251 PCNA_1; PS00293 PCNA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKATIEADIFRETIDAVSALVNECRLHVDEKGIRTITVDTSNVAMVSLELSASAFLTFA
CCEEEHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEECCCEEEEEEEECCEEEEEEE
AEPCEIGLDIEKIRSMMSMIGKTDIVSLELDESGKKLKISFGGYEYSITLLDTKTIRKDP
CCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCEEEEEEEEEEEHHCCCC
NAPNLNLPATFEVPGVMFNDAIKASSMVSDKISLAVSAETCVFTMNADGDSDRIKRELTG
CCCCCCCCEEEECCCEEEHHHHHHHHHHCCEEEEEEEEEEEEEEEECCCCHHHHHHHCCC
DDVHYLTCADARSLFSLDYLKDMGKSIGRAEKVQIRLGTDHPVQFSFVYAGGKGSVGYLL
CCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEEECCCCCEEEEE
APRIEAD
ECCCCCC
>Mature Secondary Structure
MLKATIEADIFRETIDAVSALVNECRLHVDEKGIRTITVDTSNVAMVSLELSASAFLTFA
CCEEEHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEECCCEEEEEEEECCEEEEEEE
AEPCEIGLDIEKIRSMMSMIGKTDIVSLELDESGKKLKISFGGYEYSITLLDTKTIRKDP
CCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCEEEEEEEEEEEHHCCCC
NAPNLNLPATFEVPGVMFNDAIKASSMVSDKISLAVSAETCVFTMNADGDSDRIKRELTG
CCCCCCCCEEEECCCEEEHHHHHHHHHHCCEEEEEEEEEEEEEEEECCCCHHHHHHHCCC
DDVHYLTCADARSLFSLDYLKDMGKSIGRAEKVQIRLGTDHPVQFSFVYAGGKGSVGYLL
CCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEEECCCCCEEEEE
APRIEAD
ECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA