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The map label for this gene is rpoD [H]

Identifier: 124486064

GI number: 124486064

Start: 1271510

End: 1272337

Strand: Reverse

Name: rpoD [H]

Synonym: Mlab_1244

Alternate gene names: 124486064

Gene position: 1272337-1271510 (Counterclockwise)

Preceding gene: 124486065

Following gene: 124486063

Centisome position: 70.49

GC content: 51.21

Gene sequence:

>828_bases
ATGGATCTGGTATTCAGCAGGCTCGATGACTCTGTCGCACGTTTTACTATTCACGGAGCGACTCCTGCATTCGCCAACGC
ACTTCGTCGCTCGATGATCGGTGAGGTGCCAACCCTCGCCATCGAAGACATCCGCATCTATGACAATACCAGCGTTCTCT
TTGACGAGATGCTGGCACACAGACTTGGTCTCATCCCCATTAAGACCGACCTCTCGCAGTACGTTCCGCGAAGCGAATGT
TCCTGCGGCGGCGAGGGATGTCCACACTGCACGGTAATGTTCACTCTGAGTGTTGAAGGCCCGAAGATGGTCACCTCCGG
TGATCTTATCTCCATGGACGAGAACGTCGTCCCGGTCCACAACAATATCCCGATCGTCAAACTTTGGGAAAATCAGAAGG
TCGTTTTAGAGGCAGTAGCGGAAGTCAACTACGGAAAAGAGCATGCCAAATGGCAGCCGACTCTTGCCTGCGGTTACTCA
GAATATCCGGTCATCACCATCAAAGATACCTGTGACGGCTGCGGAAAATGTGTTGCCGAATGTCCCCGCGACGTTCTGGA
ACTCACGAAAGATACCGTCAGCATCCGTGTTGTAAACGGCGAAAGCAAGGAAAAAGACTGTTCGTTATGCCGTCTCTGCG
AGACTGCATGCATGGACAGCGGCATCGGCGAAGACCCTGCTATTATTATTAGTGCAGACAGCACGAAGTTCATCTTCGTT
GTTGAAAGCGACGGGTCCTTGCCGGTGAAAGAGATCATTGAAAGAGCACTGCTGCATATCAAGTCAAGATCCTCAGATCT
TATTGAAGCATTACAGGAGGGACTGTAA

Upstream 100 bases:

>100_bases
TTCACGTGCAGGTATGCGCATCGGCAGAATCGAAGACGTAACTCCGGTACCACACGACAGCATCCGTGCCAAAGGTGGCA
GAAGGGGCAGGAGAGTCTGA

Downstream 100 bases:

>100_bases
ATGAATAAAACTAACCCACGTCTCGAATCCTTAATCATGATGCTTAAACGCGCATCCCGGGAGAACGAGGCAAACATCTG
GCGTGAAATTGCCGGCCGTC

Product: DNA-directed RNA polymerase subunit D

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MDLVFSRLDDSVARFTIHGATPAFANALRRSMIGEVPTLAIEDIRIYDNTSVLFDEMLAHRLGLIPIKTDLSQYVPRSEC
SCGGEGCPHCTVMFTLSVEGPKMVTSGDLISMDENVVPVHNNIPIVKLWENQKVVLEAVAEVNYGKEHAKWQPTLACGYS
EYPVITIKDTCDGCGKCVAECPRDVLELTKDTVSIRVVNGESKEKDCSLCRLCETACMDSGIGEDPAIIISADSTKFIFV
VESDGSLPVKEIIERALLHIKSRSSDLIEALQEGL

Sequences:

>Translated_275_residues
MDLVFSRLDDSVARFTIHGATPAFANALRRSMIGEVPTLAIEDIRIYDNTSVLFDEMLAHRLGLIPIKTDLSQYVPRSEC
SCGGEGCPHCTVMFTLSVEGPKMVTSGDLISMDENVVPVHNNIPIVKLWENQKVVLEAVAEVNYGKEHAKWQPTLACGYS
EYPVITIKDTCDGCGKCVAECPRDVLELTKDTVSIRVVNGESKEKDCSLCRLCETACMDSGIGEDPAIIISADSTKFIFV
VESDGSLPVKEIIERALLHIKSRSSDLIEALQEGL
>Mature_275_residues
MDLVFSRLDDSVARFTIHGATPAFANALRRSMIGEVPTLAIEDIRIYDNTSVLFDEMLAHRLGLIPIKTDLSQYVPRSEC
SCGGEGCPHCTVMFTLSVEGPKMVTSGDLISMDENVVPVHNNIPIVKLWENQKVVLEAVAEVNYGKEHAKWQPTLACGYS
EYPVITIKDTCDGCGKCVAECPRDVLELTKDTVSIRVVNGESKEKDCSLCRLCETACMDSGIGEDPAIIISADSTKFIFV
VESDGSLPVKEIIERALLHIKSRSSDLIEALQEGL

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the archaeal rpoD/eukaryotic RPB3 RNA polymerase subunit family [H]

Homologues:

Organism=Homo sapiens, GI14702171, Length=287, Percent_Identity=33.1010452961672, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI42560246, Length=295, Percent_Identity=27.4576271186441, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI4759046, Length=195, Percent_Identity=31.7948717948718, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI86560996, Length=299, Percent_Identity=30.4347826086957, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI25155095, Length=305, Percent_Identity=28.5245901639344, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322168, Length=279, Percent_Identity=29.7491039426523, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6325367, Length=310, Percent_Identity=27.4193548387097, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17137650, Length=186, Percent_Identity=36.0215053763441, Blast_Score=107, Evalue=7e-24,
Organism=Drosophila melanogaster, GI20129237, Length=195, Percent_Identity=31.7948717948718, Blast_Score=94, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR001514
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR022842 [H]

Pfam domain/function: PF01000 RNA_pol_A_bac; PF01193 RNA_pol_L [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 30126; Mature: 30126

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00198 4FE4S_FERREDOXIN ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
4.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLVFSRLDDSVARFTIHGATPAFANALRRSMIGEVPTLAIEDIRIYDNTSVLFDEMLAH
CCCHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHH
RLGLIPIKTDLSQYVPRSECSCGGEGCPHCTVMFTLSVEGPKMVTSGDLISMDENVVPVH
HHCCEEEECCHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEECCCEEECCCCEEEEC
NNIPIVKLWENQKVVLEAVAEVNYGKEHAKWQPTLACGYSEYPVITIKDTCDGCGKCVAE
CCCCEEEEECCCHHHHHHHHHCCCCHHHCCCCCEEEECCCCCCEEEECCCCCHHHHHHHH
CPRDVLELTKDTVSIRVVNGESKEKDCSLCRLCETACMDSGIGEDPAIIISADSTKFIFV
CCHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEE
VESDGSLPVKEIIERALLHIKSRSSDLIEALQEGL
EECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MDLVFSRLDDSVARFTIHGATPAFANALRRSMIGEVPTLAIEDIRIYDNTSVLFDEMLAH
CCCHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHH
RLGLIPIKTDLSQYVPRSECSCGGEGCPHCTVMFTLSVEGPKMVTSGDLISMDENVVPVH
HHCCEEEECCHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEECCCEEECCCCEEEEC
NNIPIVKLWENQKVVLEAVAEVNYGKEHAKWQPTLACGYSEYPVITIKDTCDGCGKCVAE
CCCCEEEEECCCHHHHHHHHHCCCCHHHCCCCCEEEECCCCCCEEEECCCCCHHHHHHHH
CPRDVLELTKDTVSIRVVNGESKEKDCSLCRLCETACMDSGIGEDPAIIISADSTKFIFV
CCHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEE
VESDGSLPVKEIIERALLHIKSRSSDLIEALQEGL
EECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]