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The map label for this gene is rpoN

Identifier: 124486060

GI number: 124486060

Start: 1270051

End: 1270239

Strand: Reverse

Name: rpoN

Synonym: Mlab_1240

Alternate gene names: 124486060

Gene position: 1270239-1270051 (Counterclockwise)

Preceding gene: 124486061

Following gene: 124486059

Centisome position: 70.37

GC content: 42.33

Gene sequence:

>189_bases
ATGATACCAGTTCGATGTTTCACCTGTGGAAAAGTAATTTCTACTGTATGGGAAGAATATCAACAGAGAAAAGCTGCAGG
CGAGGATCCGAAAGAGATTCTTGATTCACTCGGGTTAGACCGATATTGCTGCAGAAGAATGCTTCTCTCTCACAAAGAGA
CTGTTGATGAGTTGTATCCGTACACGTGA

Upstream 100 bases:

>100_bases
GCACGGTTCCAGAAGTCTTATCGTTAAGGCACAGCATACAGAATATATGGAGGGGGCAACCCCACATATCAACAATTTAA
TAAGGACGTAAAGATAACTT

Downstream 100 bases:

>100_bases
GACATGAATGAATTGCGGGGTCGTAGGGTAGCCTGGACCATCCTATTACGTTCGGGACGTAGTGACCTGAGTTCGAATCT
CAGCGACCCCATTTTTCCAT

Product: DNA-directed RNA polymerase subunit N

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 62; Mature: 62

Protein sequence:

>62_residues
MIPVRCFTCGKVISTVWEEYQQRKAAGEDPKEILDSLGLDRYCCRRMLLSHKETVDELYPYT

Sequences:

>Translated_62_residues
MIPVRCFTCGKVISTVWEEYQQRKAAGEDPKEILDSLGLDRYCCRRMLLSHKETVDELYPYT
>Mature_62_residues
MIPVRCFTCGKVISTVWEEYQQRKAAGEDPKEILDSLGLDRYCCRRMLLSHKETVDELYPYT

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG1644

COG function: function code K; DNA-directed RNA polymerase, subunit N (RpoN/RPB10)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the archaeal rpoN/eukaryotic RPB10 RNA polymerase subunit family

Homologues:

Organism=Homo sapiens, GI10863925, Length=62, Percent_Identity=50, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17509857, Length=61, Percent_Identity=50.8196721311475, Blast_Score=69, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324784, Length=63, Percent_Identity=44.4444444444444, Blast_Score=62, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24649759, Length=62, Percent_Identity=51.6129032258064, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RPON_METLZ (A2SSV3)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001030676.1
- ProteinModelPortal:   A2SSV3
- SMR:   A2SSV3
- STRING:   A2SSV3
- GeneID:   4794920
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_1240
- eggNOG:   arNOG12678
- HOGENOM:   HBG391971
- OMA:   KYCCRRM
- PhylomeDB:   A2SSV3
- ProtClustDB:   PRK04016
- BioCyc:   MLAB410358:MLAB_1240-MONOMER
- HAMAP:   MF_00250
- InterPro:   IPR012287
- InterPro:   IPR000268
- InterPro:   IPR020789
- Gene3D:   G3DSA:1.10.10.60
- PANTHER:   PTHR23413:SF2
- PIRSF:   PIRSF005653
- ProDom:   PD006539

Pfam domain/function: PF01194 RNA_pol_N; SSF46924 RNA_pol_N/8_sub

EC number: =2.7.7.6

Molecular weight: Translated: 7286; Mature: 7286

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS01112 RNA_POL_N_8KD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
9.7 %Cys+Met (Translated Protein)
6.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
9.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPVRCFTCGKVISTVWEEYQQRKAAGEDPKEILDSLGLDRYCCRRMLLSHKETVDELYP
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
YT
CC
>Mature Secondary Structure
MIPVRCFTCGKVISTVWEEYQQRKAAGEDPKEILDSLGLDRYCCRRMLLSHKETVDELYP
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
YT
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA