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The map label for this gene is eno

Identifier: 124486058

GI number: 124486058

Start: 1268532

End: 1269731

Strand: Reverse

Name: eno

Synonym: Mlab_1238

Alternate gene names: 124486058

Gene position: 1269731-1268532 (Counterclockwise)

Preceding gene: 124486059

Following gene: 124486057

Centisome position: 70.35

GC content: 53.33

Gene sequence:

>1200_bases
ATGACTGAAATTTCCGACATTCTTCTTCGGACAATTCTGGACTCACGCGGCAATCCGACCGTCGAAGCCGAGATCTCCAC
GATCTCCGGCGGATTCGGTCGTGCCTGCGCTCCGAGCGGAGCCTCTACCGGTATCTATGAGGCAAAAGTCCGTCCGTGTG
ACGAGGCCGTGGCCGATGCCTATATCAACCTCATCCCCAAACTGATCGAACTTGATTCTGCCGATCAGCGCGGATTCGAT
GACACCCTGCATGAAGTCGACGGAACGTCCGATCTTTCCGGAATCGGTGCCAACATCGCCGTGGCTCTGTCCCTTGCCAA
CGCCAAAGCAGCGGCAAGCACACTCAACATGGAACTCTATCAGTACCTCGGCGGCGCTTTTATCTCCCAGACCCCGCTGC
CGTTAGGAAATGTTATCGGGGGCGGCGCTCATGCCGTTGACGCAACCGATATTCAGGAGTTCCTTATCGTGCCGACCGGC
GCATCCACTGCTGCTGAAGCCGTATTCACCAATGCCCTCGTCCACAAACGCATAAAAGAGATTCTTATCGCGCGCGGAAA
AGGCTGCGGTAAAGGCGACGAGGGAGGATGGGCTCCGCACATCGCCGACCTTGAAGCCTTTGAAATCGTGAACGAAGCAA
CCACCAAAGTCTTTGACGAGACCGGCATCGAAGTCCGCATGGGACTTGACGTTGCCGCATCGGAGATGTGGGATGCCGCA
AGCGGTAGATACGTCTATAAGAATGCAAAGCGAACTACTGAGGAGCAGATTGCATACATTGCCGACCTGACCGAAAAATA
CAACCTGATCTATGTCGAAGACCCGATTCAGGAAGAGGATTTCGAAGGGTTTGCACGTATCACCGAAGAAGTCTCCGGCC
GCGACACTCTTATCTGCGGTGATGACCTGTTCGTTACGAATGCTTCCCGTCTGGAAGAAGGTATCAGAAATGATGCCTGC
AACTGTGTCCTGATCAAACCAAACCAGATCGGAACGCTGACGGACACCTTCGAAACGATCAGTCTCGCCCATGATTACGG
GTATGAAACCGTCATGAGCCACCGATCCGGTGAAACGACCGACAACACCATCGCTCATCTTGCAACGGCATTCGGCTGCT
GCTTTATGAAGAGCGGTGTTGTTGGTGGAGAGCGCATTGCTAAACTAAATGAACTTATAAGAATTGAGGAGCATTTCTAA

Upstream 100 bases:

>100_bases
AGATTGATCCTCTTGACATTGCGTTAGAGGAGTTCGACGCTGATCGGGTCCCGATAACGGTGAAACGCCAGTTCGGTACC
TGCTCAGAGGTGCTTTAAGT

Downstream 100 bases:

>100_bases
AATGACCGACAATGAACTTGGAATTGAATTAAACGAACCATTAGTATCTGTAGAAGAATATCTTGCCGCCGGTGTCCATA
TCGGAACCCAGCAGAAAGAC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTEISDILLRTILDSRGNPTVEAEISTISGGFGRACAPSGASTGIYEAKVRPCDEAVADAYINLIPKLIELDSADQRGFD
DTLHEVDGTSDLSGIGANIAVALSLANAKAAASTLNMELYQYLGGAFISQTPLPLGNVIGGGAHAVDATDIQEFLIVPTG
ASTAAEAVFTNALVHKRIKEILIARGKGCGKGDEGGWAPHIADLEAFEIVNEATTKVFDETGIEVRMGLDVAASEMWDAA
SGRYVYKNAKRTTEEQIAYIADLTEKYNLIYVEDPIQEEDFEGFARITEEVSGRDTLICGDDLFVTNASRLEEGIRNDAC
NCVLIKPNQIGTLTDTFETISLAHDYGYETVMSHRSGETTDNTIAHLATAFGCCFMKSGVVGGERIAKLNELIRIEEHF

Sequences:

>Translated_399_residues
MTEISDILLRTILDSRGNPTVEAEISTISGGFGRACAPSGASTGIYEAKVRPCDEAVADAYINLIPKLIELDSADQRGFD
DTLHEVDGTSDLSGIGANIAVALSLANAKAAASTLNMELYQYLGGAFISQTPLPLGNVIGGGAHAVDATDIQEFLIVPTG
ASTAAEAVFTNALVHKRIKEILIARGKGCGKGDEGGWAPHIADLEAFEIVNEATTKVFDETGIEVRMGLDVAASEMWDAA
SGRYVYKNAKRTTEEQIAYIADLTEKYNLIYVEDPIQEEDFEGFARITEEVSGRDTLICGDDLFVTNASRLEEGIRNDAC
NCVLIKPNQIGTLTDTFETISLAHDYGYETVMSHRSGETTDNTIAHLATAFGCCFMKSGVVGGERIAKLNELIRIEEHF
>Mature_398_residues
TEISDILLRTILDSRGNPTVEAEISTISGGFGRACAPSGASTGIYEAKVRPCDEAVADAYINLIPKLIELDSADQRGFDD
TLHEVDGTSDLSGIGANIAVALSLANAKAAASTLNMELYQYLGGAFISQTPLPLGNVIGGGAHAVDATDIQEFLIVPTGA
STAAEAVFTNALVHKRIKEILIARGKGCGKGDEGGWAPHIADLEAFEIVNEATTKVFDETGIEVRMGLDVAASEMWDAAS
GRYVYKNAKRTTEEQIAYIADLTEKYNLIYVEDPIQEEDFEGFARITEEVSGRDTLICGDDLFVTNASRLEEGIRNDACN
CVLIKPNQIGTLTDTFETISLAHDYGYETVMSHRSGETTDNTIAHLATAFGCCFMKSGVVGGERIAKLNELIRIEEHF

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the archaeal cel

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=422, Percent_Identity=38.8625592417062, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI301897477, Length=419, Percent_Identity=38.6634844868735, Blast_Score=235, Evalue=5e-62,
Organism=Homo sapiens, GI301897469, Length=419, Percent_Identity=38.6634844868735, Blast_Score=235, Evalue=5e-62,
Organism=Homo sapiens, GI4503571, Length=421, Percent_Identity=36.5795724465558, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI301897479, Length=406, Percent_Identity=36.6995073891626, Blast_Score=212, Evalue=6e-55,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=26.1904761904762, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=26.1904761904762, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=26.1904761904762, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1789141, Length=417, Percent_Identity=42.6858513189448, Blast_Score=277, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17536383, Length=422, Percent_Identity=37.4407582938389, Blast_Score=229, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI71995829, Length=422, Percent_Identity=37.4407582938389, Blast_Score=229, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI32563855, Length=183, Percent_Identity=33.879781420765, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321693, Length=424, Percent_Identity=37.0283018867925, Blast_Score=223, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6323985, Length=429, Percent_Identity=37.0629370629371, Blast_Score=222, Evalue=8e-59,
Organism=Saccharomyces cerevisiae, GI6324974, Length=428, Percent_Identity=37.6168224299065, Blast_Score=222, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6324969, Length=428, Percent_Identity=37.6168224299065, Blast_Score=222, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6321968, Length=424, Percent_Identity=36.5566037735849, Blast_Score=215, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24580918, Length=420, Percent_Identity=37.8571428571429, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24580916, Length=420, Percent_Identity=37.8571428571429, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24580920, Length=420, Percent_Identity=37.8571428571429, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24580914, Length=420, Percent_Identity=37.8571428571429, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI281360527, Length=420, Percent_Identity=37.8571428571429, Blast_Score=226, Evalue=1e-59,
Organism=Drosophila melanogaster, GI17137654, Length=420, Percent_Identity=37.8571428571429, Blast_Score=226, Evalue=1e-59,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_METLZ (A2SSV1)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001030674.1
- ProteinModelPortal:   A2SSV1
- SMR:   A2SSV1
- STRING:   A2SSV1
- GeneID:   4795183
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_1238
- eggNOG:   arNOG04113
- HOGENOM:   HBG726599
- OMA:   LICGDDL
- ProtClustDB:   PRK00077
- BioCyc:   MLAB410358:MLAB_1238-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 42847; Mature: 42716

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 194-194 ACT_SITE 326-326 BINDING 144-144 BINDING 153-153 BINDING 273-273 BINDING 301-301 BINDING 326-326 BINDING 377-377

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEISDILLRTILDSRGNPTVEAEISTISGGFGRACAPSGASTGIYEAKVRPCDEAVADA
CCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHHHH
YINLIPKLIELDSADQRGFDDTLHEVDGTSDLSGIGANIAVALSLANAKAAASTLNMELY
HHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHH
QYLGGAFISQTPLPLGNVIGGGAHAVDATDIQEFLIVPTGASTAAEAVFTNALVHKRIKE
HHHCCHHHCCCCCCHHHHCCCCCCCCCHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
ILIARGKGCGKGDEGGWAPHIADLEAFEIVNEATTKVFDETGIEVRMGLDVAASEMWDAA
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCC
SGRYVYKNAKRTTEEQIAYIADLTEKYNLIYVEDPIQEEDFEGFARITEEVSGRDTLICG
CCCEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEC
DDLFVTNASRLEEGIRNDACNCVLIKPNQIGTLTDTFETISLAHDYGYETVMSHRSGETT
CCEEECCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCC
DNTIAHLATAFGCCFMKSGVVGGERIAKLNELIRIEEHF
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TEISDILLRTILDSRGNPTVEAEISTISGGFGRACAPSGASTGIYEAKVRPCDEAVADA
CHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHHHH
YINLIPKLIELDSADQRGFDDTLHEVDGTSDLSGIGANIAVALSLANAKAAASTLNMELY
HHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHH
QYLGGAFISQTPLPLGNVIGGGAHAVDATDIQEFLIVPTGASTAAEAVFTNALVHKRIKE
HHHCCHHHCCCCCCHHHHCCCCCCCCCHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
ILIARGKGCGKGDEGGWAPHIADLEAFEIVNEATTKVFDETGIEVRMGLDVAASEMWDAA
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCC
SGRYVYKNAKRTTEEQIAYIADLTEKYNLIYVEDPIQEEDFEGFARITEEVSGRDTLICG
CCCEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEC
DDLFVTNASRLEEGIRNDACNCVLIKPNQIGTLTDTFETISLAHDYGYETVMSHRSGETT
CCEEECCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCC
DNTIAHLATAFGCCFMKSGVVGGERIAKLNELIRIEEHF
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA