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The map label for this gene is gph [C]

Identifier: 124486010

GI number: 124486010

Start: 1217394

End: 1218080

Strand: Reverse

Name: gph [C]

Synonym: Mlab_1190

Alternate gene names: 124486010

Gene position: 1218080-1217394 (Counterclockwise)

Preceding gene: 124486012

Following gene: 124486009

Centisome position: 67.49

GC content: 56.33

Gene sequence:

>687_bases
ATGCAGACCCTGCTCTCCGCTCCGTTCAAAGCCGTCCTTTTCGATATGGACAATACGCTTTTCGATTTTGTGGAGGCGAT
GAAAAAGGGCTGTGATGCGGCCGCCGCTTCGGTCGGGGAAGGGACCGGGGAGGAGCTTTTGAGCTATTATCTGCGGTGGA
AGTATCATTTCGAGGACCACACGAATCTGCAGGATTTCATGATGGCCCACAACCGCTTTTCGGTGGAGAAGTATTTCGAG
GCGGTCGGCGCTTTCGACGAGGCAAAGATGAACTGCCTGACGGCATACCCCGGGATCGAAGGGGTCCTGACCGGTCTGCG
GGACGCCGGATACCGCCTTGGGGTCGTAACGGATGCGTTTTCCTACGCGGCCGAACTGCGTCTCGAACATCTGGGTCTGA
AGTCCTACTTCGATGTGATTGTGGGTTACGACACGACCGGGTACAAAAAACCGCATTTCGCTCCGTTCGAGTGCGCTCTG
GATCTTCTCGGTCTTGCCCCACACGAGGCGGCCTATGTCGGCGACTCGATCCGGCGCGACATCGAACCGGTCAAGTCTCT
GGGTATGACGGCCGTTTATGCGAAGTACGGGGATCGGAACTTTTTCGATCATCCGTCGCTTGCCTGCCCGCAGAAAGTTC
TCGTGGCGGACACACCTTTGGATATTCTGCGGCTTTTACGACCATAA

Upstream 100 bases:

>100_bases
CCTCAGCAGCCATTTATATCTATTTAGTGGGCGGTGCCCGATATAGCTTTTGACTCTCGATTCGATATCCCTAAGTATTT
CCGGGTGAGATGTGTATTCA

Downstream 100 bases:

>100_bases
ATATAGTAATGGTTCATTGCCAATATGATAAAAACACATGATGCTTGATACAGATTTTTTAGAACGTATCGCTCCTTATA
TTGAACCCCTTACGGCCGAG

Product: hypothetical protein

Products: Glycolate; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MQTLLSAPFKAVLFDMDNTLFDFVEAMKKGCDAAAASVGEGTGEELLSYYLRWKYHFEDHTNLQDFMMAHNRFSVEKYFE
AVGAFDEAKMNCLTAYPGIEGVLTGLRDAGYRLGVVTDAFSYAAELRLEHLGLKSYFDVIVGYDTTGYKKPHFAPFECAL
DLLGLAPHEAAYVGDSIRRDIEPVKSLGMTAVYAKYGDRNFFDHPSLACPQKVLVADTPLDILRLLRP

Sequences:

>Translated_228_residues
MQTLLSAPFKAVLFDMDNTLFDFVEAMKKGCDAAAASVGEGTGEELLSYYLRWKYHFEDHTNLQDFMMAHNRFSVEKYFE
AVGAFDEAKMNCLTAYPGIEGVLTGLRDAGYRLGVVTDAFSYAAELRLEHLGLKSYFDVIVGYDTTGYKKPHFAPFECAL
DLLGLAPHEAAYVGDSIRRDIEPVKSLGMTAVYAKYGDRNFFDHPSLACPQKVLVADTPLDILRLLRP
>Mature_228_residues
MQTLLSAPFKAVLFDMDNTLFDFVEAMKKGCDAAAASVGEGTGEELLSYYLRWKYHFEDHTNLQDFMMAHNRFSVEKYFE
AVGAFDEAKMNCLTAYPGIEGVLTGLRDAGYRLGVVTDAFSYAAELRLEHLGLKSYFDVIVGYDTTGYKKPHFAPFECAL
DLLGLAPHEAAYVGDSIRRDIEPVKSLGMTAVYAKYGDRNFFDHPSLACPQKVLVADTPLDILRLLRP

Specific function: Unknown

COG id: COG1011

COG function: function code R; Predicted hydrolase (HAD superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=211, Percent_Identity=27.9620853080569, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1790833, Length=182, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR011950
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: 3.1.3.18 [C]

Molecular weight: Translated: 25480; Mature: 25480

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTLLSAPFKAVLFDMDNTLFDFVEAMKKGCDAAAASVGEGTGEELLSYYLRWKYHFEDH
CCCHHHCCHHHHHHHCCHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHEECCCC
TNLQDFMMAHNRFSVEKYFEAVGAFDEAKMNCLTAYPGIEGVLTGLRDAGYRLGVVTDAF
CCHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEHHHHHH
SYAAELRLEHLGLKSYFDVIVGYDTTGYKKPHFAPFECALDLLGLAPHEAAYVGDSIRRD
HHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
IEPVKSLGMTAVYAKYGDRNFFDHPSLACPQKVLVADTPLDILRLLRP
HHHHHHHCHHHEEHHHCCCCCCCCCCCCCCCCEEEECCCHHHHHHHCC
>Mature Secondary Structure
MQTLLSAPFKAVLFDMDNTLFDFVEAMKKGCDAAAASVGEGTGEELLSYYLRWKYHFEDH
CCCHHHCCHHHHHHHCCHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHEECCCC
TNLQDFMMAHNRFSVEKYFEAVGAFDEAKMNCLTAYPGIEGVLTGLRDAGYRLGVVTDAF
CCHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEHHHHHH
SYAAELRLEHLGLKSYFDVIVGYDTTGYKKPHFAPFECALDLLGLAPHEAAYVGDSIRRD
HHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
IEPVKSLGMTAVYAKYGDRNFFDHPSLACPQKVLVADTPLDILRLLRP
HHHHHHHCHHHEEHHHCCCCCCCCCCCCCCCCEEEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.57 {phosphoglycolate}} [C]

Substrates: D-Glycerate 2-phosphatelyc; H2O [C]

Specific reaction: D-Glycerate 2-phosphatelyc + H2O --> Glycolate + Phosphate [C]

General reaction: Additional information:

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]