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The map label for this gene is vsr [H]

Identifier: 124486000

GI number: 124486000

Start: 1204007

End: 1204462

Strand: Reverse

Name: vsr [H]

Synonym: Mlab_1180

Alternate gene names: 124486000

Gene position: 1204462-1204007 (Counterclockwise)

Preceding gene: 124486005

Following gene: 124485997

Centisome position: 66.73

GC content: 43.86

Gene sequence:

>456_bases
ATGGATAGAGTAACTCCCGAACACCGTAGTTGGATGATGAGTCGAGTTCGGTCAAAGAAAACTAAACCGGAACAACTCGT
TGCTTCCTATCTGAGGGAATCTCATATCGGCTATCGACGATGTCGGACCGATCTTCCGGGAAAACCGGACTTCGTTCTTT
CCAAATATCGAGCCGTCATTTTTGTGAATGGATGTTTTTGGCATGGTCATGCGGGGTGCAAACGTGCCACCATTCCCAAA
ACGAATACGGAGTGGTGGATTGCTAAATTTCAACAAAATAAATTACGCGATGAGAGAAATTATGCACAGCTTCGGGAAAT
GGGGTGGCGGGTTTTCGTAATTTGGGAATGTGAACTTGGGAACGATGTGGAATATAAACTCAAGGAATTAGTGAAGGAAA
TGCTGCTTTGGGATGATTATCCTGCTGATGACTATCCAGACGAAATATTCTACTAA

Upstream 100 bases:

>100_bases
AATGGATATATGCATTAAGCTATGATGAGTTGGGAAATCCCGGCTTACGTGGTTATGTTTGCAGCATAGTAAAATCAAAC
AAATCGATAAATAATTCACC

Downstream 100 bases:

>100_bases
TCAAAAATCCATAGGTCGGTTTCCATTCCTGCATCCTATAATGAGGATTCTCCACCGCGTCGGGCTCCACGCCGGAGTCG
CCTTCCCGCACCCCAGTCAG

Product: T/G mismatch-specific endonuclease

Products: DNA [C]

Alternate protein names: V.XorII [H]

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MDRVTPEHRSWMMSRVRSKKTKPEQLVASYLRESHIGYRRCRTDLPGKPDFVLSKYRAVIFVNGCFWHGHAGCKRATIPK
TNTEWWIAKFQQNKLRDERNYAQLREMGWRVFVIWECELGNDVEYKLKELVKEMLLWDDYPADDYPDEIFY

Sequences:

>Translated_151_residues
MDRVTPEHRSWMMSRVRSKKTKPEQLVASYLRESHIGYRRCRTDLPGKPDFVLSKYRAVIFVNGCFWHGHAGCKRATIPK
TNTEWWIAKFQQNKLRDERNYAQLREMGWRVFVIWECELGNDVEYKLKELVKEMLLWDDYPADDYPDEIFY
>Mature_151_residues
MDRVTPEHRSWMMSRVRSKKTKPEQLVASYLRESHIGYRRCRTDLPGKPDFVLSKYRAVIFVNGCFWHGHAGCKRATIPK
TNTEWWIAKFQQNKLRDERNYAQLREMGWRVFVIWECELGNDVEYKLKELVKEMLLWDDYPADDYPDEIFY

Specific function: May nick XorII sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=111, Percent_Identity=43.2432432432432, Blast_Score=105, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 18256; Mature: 18256

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRVTPEHRSWMMSRVRSKKTKPEQLVASYLRESHIGYRRCRTDLPGKPDFVLSKYRAVI
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHCCEEEE
FVNGCFWHGHAGCKRATIPKTNTEWWIAKFQQNKLRDERNYAQLREMGWRVFVIWECELG
EEECCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
NDVEYKLKELVKEMLLWDDYPADDYPDEIFY
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDRVTPEHRSWMMSRVRSKKTKPEQLVASYLRESHIGYRRCRTDLPGKPDFVLSKYRAVI
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHCCEEEE
FVNGCFWHGHAGCKRATIPKTNTEWWIAKFQQNKLRDERNYAQLREMGWRVFVIWECELG
EEECCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
NDVEYKLKELVKEMLLWDDYPADDYPDEIFY
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8078464 [H]