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The map label for this gene is purB [H]

Identifier: 124485941

GI number: 124485941

Start: 1132134

End: 1133477

Strand: Reverse

Name: purB [H]

Synonym: Mlab_1121

Alternate gene names: 124485941

Gene position: 1133477-1132134 (Counterclockwise)

Preceding gene: 124485943

Following gene: 124485938

Centisome position: 62.8

GC content: 56.18

Gene sequence:

>1344_bases
ATGGCAATACATCCGATAGATTTCCGGTACGGAACTCCTGAAATGAAACGTGTGTGGAGTGAAGACAACCGGTTTACCTG
CATTGTCAGGGCCGAGGTTGCTCTGGCCCATGCTCAGGGAGTCGTCGGGATGATCCCGGCGGAGGATGCGGCGATCATCG
AGAAAAACGCTCTTTCGGCTTCACGCGTCCGTGCAAAAGAGATCGAGGCGGAGATCAGCCACGATACGATGGCAATAACC
CGGGCAATTTCGGAGGTCTGCGGGGATGCGGGACGCTGGATCCATTACGGGGCCACCTCGAACGATATTCTGGATACGGC
GACCGGTCTTCAGCTCAAAGAGGCGTATGATCTCTTAGAGAAAAAACTATCAACGCTGATGGGTGTGTTGTTGAAACGTG
CCGAGGAGACCAAAAATCTGATCTGCATTGCCCGGACCCACGGTCAGCAGGGTGTGCCGACGACCTACGGCCTTCGGTTT
GCGATCTGGGCGTCCGAGGTCTCACGGCATCTCATAAGACTAAGAGAGAGCCGGCCGCGTGTGGTCGTCGGGCAGATGAC
CGGAGCGGTTGGAACGATGGCTGCCATGGGCGAACACGGCATTGAAGTCCAGGCTGAGATGATGAAGTATCTGGGCATTG
GATCAGTGGATGTCTCGTCCCAGGTCATCTCACGCGACCGGTATGCCGAGTACATCTTCCTGCTCGCAAACATCGCAACG
ACGCTGGACAAAGTGGGAATTGAGGTCAGGACCCTGCAGCGGACGGAGATCGGCGAGGTCGAAGAGTCCTTCTCCAAAAA
GCAGGTAGGATCTTCCACGATGCCGCATAAAAGAAATCCGATCAAGAGCGAGCAGGTCTGCGGTCTTGCACGGATCGTCC
GCAGTATGGTCGAGCCGGCTCTTTTGAACAATACGCTCTGGGACGAACGGGATCTGACGAACTCGTCGGCCGAGCGTATC
ACCTTCCTCGAGACCAGCATTCTGTGCGATCACTGTCTGCAGGTGATGACGGGCGTCATCGAAAATCTGACCATCAACAC
GGAAGCGGTCGAACGAAACCTCAGATATCTGCACGGCATCAATCTTGCCGAGTCGGTGATGATCGAGCTGACGAAACGCG
GGATGAAGCGCGAGGATGCGCACGAGACCATCCGCGAGGCAAGCATGGAGGCGCTTTCTGCAAAGATGCCGCTTGCCGAG
ATCCTTGCGGCAAAGCCGGAGGTCACTGAGTATGTCTCGGCTGAAGAGATCGCAAAACTGCTCAAGCCCGAAAGCTACGC
CGGGCTTGCGGGCGTTCAGGTGGAAAATCTGATCCGAAAACTTGCGCCGTACTGCACGCTGTAA

Upstream 100 bases:

>100_bases
TTGTTTTCCGTGGTGAAAAAGAACTGCCCCGGGGACATTCCCAGCGTTTCTATTCTACAGGACAAACTCATAACCAAAGA
AAACCAATACTAGTTTTACT

Downstream 100 bases:

>100_bases
TCGTGTCCTGAAAACAAGCGGGAAGTATTATTCCTCTCTTCTTTTTTTATCACTTAACCGGTATGTCCCAGTCTCTTCTC
TCACGAAAAACCCTTCGGCC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MAIHPIDFRYGTPEMKRVWSEDNRFTCIVRAEVALAHAQGVVGMIPAEDAAIIEKNALSASRVRAKEIEAEISHDTMAIT
RAISEVCGDAGRWIHYGATSNDILDTATGLQLKEAYDLLEKKLSTLMGVLLKRAEETKNLICIARTHGQQGVPTTYGLRF
AIWASEVSRHLIRLRESRPRVVVGQMTGAVGTMAAMGEHGIEVQAEMMKYLGIGSVDVSSQVISRDRYAEYIFLLANIAT
TLDKVGIEVRTLQRTEIGEVEESFSKKQVGSSTMPHKRNPIKSEQVCGLARIVRSMVEPALLNNTLWDERDLTNSSAERI
TFLETSILCDHCLQVMTGVIENLTINTEAVERNLRYLHGINLAESVMIELTKRGMKREDAHETIREASMEALSAKMPLAE
ILAAKPEVTEYVSAEEIAKLLKPESYAGLAGVQVENLIRKLAPYCTL

Sequences:

>Translated_447_residues
MAIHPIDFRYGTPEMKRVWSEDNRFTCIVRAEVALAHAQGVVGMIPAEDAAIIEKNALSASRVRAKEIEAEISHDTMAIT
RAISEVCGDAGRWIHYGATSNDILDTATGLQLKEAYDLLEKKLSTLMGVLLKRAEETKNLICIARTHGQQGVPTTYGLRF
AIWASEVSRHLIRLRESRPRVVVGQMTGAVGTMAAMGEHGIEVQAEMMKYLGIGSVDVSSQVISRDRYAEYIFLLANIAT
TLDKVGIEVRTLQRTEIGEVEESFSKKQVGSSTMPHKRNPIKSEQVCGLARIVRSMVEPALLNNTLWDERDLTNSSAERI
TFLETSILCDHCLQVMTGVIENLTINTEAVERNLRYLHGINLAESVMIELTKRGMKREDAHETIREASMEALSAKMPLAE
ILAAKPEVTEYVSAEEIAKLLKPESYAGLAGVQVENLIRKLAPYCTL
>Mature_446_residues
AIHPIDFRYGTPEMKRVWSEDNRFTCIVRAEVALAHAQGVVGMIPAEDAAIIEKNALSASRVRAKEIEAEISHDTMAITR
AISEVCGDAGRWIHYGATSNDILDTATGLQLKEAYDLLEKKLSTLMGVLLKRAEETKNLICIARTHGQQGVPTTYGLRFA
IWASEVSRHLIRLRESRPRVVVGQMTGAVGTMAAMGEHGIEVQAEMMKYLGIGSVDVSSQVISRDRYAEYIFLLANIATT
LDKVGIEVRTLQRTEIGEVEESFSKKQVGSSTMPHKRNPIKSEQVCGLARIVRSMVEPALLNNTLWDERDLTNSSAERIT
FLETSILCDHCLQVMTGVIENLTINTEAVERNLRYLHGINLAESVMIELTKRGMKREDAHETIREASMEALSAKMPLAEI
LAAKPEVTEYVSAEEIAKLLKPESYAGLAGVQVENLIRKLAPYCTL

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=461, Percent_Identity=29.7180043383948, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI183227688, Length=386, Percent_Identity=29.5336787564767, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI31541964, Length=337, Percent_Identity=24.9258160237389, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI68303542, Length=337, Percent_Identity=24.9258160237389, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1787376, Length=344, Percent_Identity=27.906976744186, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790398, Length=404, Percent_Identity=23.5148514851485, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508577, Length=389, Percent_Identity=28.5347043701799, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI32564234, Length=318, Percent_Identity=29.559748427673, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17553882, Length=275, Percent_Identity=26.1818181818182, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI32565146, Length=270, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6323391, Length=455, Percent_Identity=29.4505494505495, Blast_Score=171, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24647570, Length=456, Percent_Identity=28.7280701754386, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24640179, Length=206, Percent_Identity=29.126213592233, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24640177, Length=206, Percent_Identity=29.126213592233, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49539; Mature: 49408

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIHPIDFRYGTPEMKRVWSEDNRFTCIVRAEVALAHAQGVVGMIPAEDAAIIEKNALSA
CCCCCCCCCCCCHHHHHHHCCCCCEEEEEEHHHHHHHHCCCEEECCCCCHHHHHHCCCCH
SRVRAKEIEAEISHDTMAITRAISEVCGDAGRWIHYGATSNDILDTATGLQLKEAYDLLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHCCCHHHHHHHHHH
KKLSTLMGVLLKRAEETKNLICIARTHGQQGVPTTYGLRFAIWASEVSRHLIRLRESRPR
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHCCCC
VVVGQMTGAVGTMAAMGEHGIEVQAEMMKYLGIGSVDVSSQVISRDRYAEYIFLLANIAT
EEEEEHHCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TLDKVGIEVRTLQRTEIGEVEESFSKKQVGSSTMPHKRNPIKSEQVCGLARIVRSMVEPA
HHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LLNNTLWDERDLTNSSAERITFLETSILCDHCLQVMTGVIENLTINTEAVERNLRYLHGI
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
NLAESVMIELTKRGMKREDAHETIREASMEALSAKMPLAEILAAKPEVTEYVSAEEIAKL
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHCCHHHHHHH
LKPESYAGLAGVQVENLIRKLAPYCTL
HCCCCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AIHPIDFRYGTPEMKRVWSEDNRFTCIVRAEVALAHAQGVVGMIPAEDAAIIEKNALSA
CCCCCCCCCCCHHHHHHHCCCCCEEEEEEHHHHHHHHCCCEEECCCCCHHHHHHCCCCH
SRVRAKEIEAEISHDTMAITRAISEVCGDAGRWIHYGATSNDILDTATGLQLKEAYDLLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHCCCHHHHHHHHHH
KKLSTLMGVLLKRAEETKNLICIARTHGQQGVPTTYGLRFAIWASEVSRHLIRLRESRPR
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHCCCC
VVVGQMTGAVGTMAAMGEHGIEVQAEMMKYLGIGSVDVSSQVISRDRYAEYIFLLANIAT
EEEEEHHCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TLDKVGIEVRTLQRTEIGEVEESFSKKQVGSSTMPHKRNPIKSEQVCGLARIVRSMVEPA
HHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LLNNTLWDERDLTNSSAERITFLETSILCDHCLQVMTGVIENLTINTEAVERNLRYLHGI
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
NLAESVMIELTKRGMKREDAHETIREASMEALSAKMPLAEILAAKPEVTEYVSAEEIAKL
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHCCHHHHHHH
LKPESYAGLAGVQVENLIRKLAPYCTL
HCCCCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]