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The map label for this gene is nifH [H]

Identifier: 124485861

GI number: 124485861

Start: 1051819

End: 1052565

Strand: Reverse

Name: nifH [H]

Synonym: Mlab_1041

Alternate gene names: 124485861

Gene position: 1052565-1051819 (Counterclockwise)

Preceding gene: 124485862

Following gene: 124485860

Centisome position: 58.32

GC content: 43.11

Gene sequence:

>747_bases
ATGAAAAAAATTGCATTTTATGGTAAAGGCGGAATCGGAAAATCAACCTGTGTCTCAAATATTTCTATGGCTCTCGCCGA
GCAGGGATTCACGGTCATGCAGATCGGCTGCGATCCGAAAGCAGATTCAACGAGAAATCTAACTCACGGAGATTTGATCC
CATCTGTTCTCTCAGTAATAAGGGAGAAAAAGAATGATATTTGTTTGGAAGATATCGTTTCCTGTGGATCAGGTGGAGTA
TTGTGTGTAGAGGCGGGGGGGCCAAAACCCGGGTCAGGTTGTGCAGGTCGCGGCATTATTACTGCTTTTGAACAATTGGA
GGAGCTTGGAGCTTATACTACCTATCATCCAGATATTATTCTCTATGATGTTTTAGGAGATGTGGTCTGTGGAGGATTTG
CCCTGCCGATACGCCAAGGATATGCCGATGAGATCTGTATTGTGACATCCGGCGAGATGATGTCCTTGTATGCCGCGGAT
AACATCTCTCAGGCGGTGAATAATTATTCAAAGCGTGGATATGCGGCATTGAAGGGACTCATCTACAATGCAAAAAATAT
TGAAAATGAAGATGAGATTGTGGAAAAAGCGGCTGCAGAAATGAATACAAAGGTGTTGTCCAGGATTAACCGTGACCCGA
TTGTCCAAAAGGCTGAAAATGCTGGTATGACTGTTGTGGAATATGCACCGAATTCATCTCAAGCAGATTTGTATAGAAAA
CTAGCAGGAATTTTACTTGAGGATTAA

Upstream 100 bases:

>100_bases
GATGCATTTCGGGTGAATGTGATGATTGGAAATGTCTCTGACGGAACAAAAACCCACCAATACATATTACCAATATCCAT
TAGAAAATAAGGAGCTATGA

Downstream 100 bases:

>100_bases
CTATGAATAAACTCTGTTTCTGCTCAACAACCTATGGAGGGTATGGGATCGTAAGGGTCGGTTCGTTAATTCCGGAAAGT
TATCTATTGTTTGTCTGCCC

Product: nitrogenase iron protein subunit NifH

Products: NA

Alternate protein names: Nitrogenase Fe protein; Nitrogenase component II; Nitrogenase reductase [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MKKIAFYGKGGIGKSTCVSNISMALAEQGFTVMQIGCDPKADSTRNLTHGDLIPSVLSVIREKKNDICLEDIVSCGSGGV
LCVEAGGPKPGSGCAGRGIITAFEQLEELGAYTTYHPDIILYDVLGDVVCGGFALPIRQGYADEICIVTSGEMMSLYAAD
NISQAVNNYSKRGYAALKGLIYNAKNIENEDEIVEKAAAEMNTKVLSRINRDPIVQKAENAGMTVVEYAPNSSQADLYRK
LAGILLED

Sequences:

>Translated_248_residues
MKKIAFYGKGGIGKSTCVSNISMALAEQGFTVMQIGCDPKADSTRNLTHGDLIPSVLSVIREKKNDICLEDIVSCGSGGV
LCVEAGGPKPGSGCAGRGIITAFEQLEELGAYTTYHPDIILYDVLGDVVCGGFALPIRQGYADEICIVTSGEMMSLYAAD
NISQAVNNYSKRGYAALKGLIYNAKNIENEDEIVEKAAAEMNTKVLSRINRDPIVQKAENAGMTVVEYAPNSSQADLYRK
LAGILLED
>Mature_248_residues
MKKIAFYGKGGIGKSTCVSNISMALAEQGFTVMQIGCDPKADSTRNLTHGDLIPSVLSVIREKKNDICLEDIVSCGSGGV
LCVEAGGPKPGSGCAGRGIITAFEQLEELGAYTTYHPDIILYDVLGDVVCGGFALPIRQGYADEICIVTSGEMMSLYAAD
NISQAVNNYSKRGYAALKGLIYNAKNIENEDEIVEKAAAEMNTKVLSRINRDPIVQKAENAGMTVVEYAPNSSQADLYRK
LAGILLED

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein and the molybdenum-iron protein [H]

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000392
- InterPro:   IPR005977 [H]

Pfam domain/function: PF00142 Fer4_NifH [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 26474; Mature: 26474

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00746 NIFH_FRXC_1 ; PS00692 NIFH_FRXC_2 ; PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIAFYGKGGIGKSTCVSNISMALAEQGFTVMQIGCDPKADSTRNLTHGDLIPSVLSVI
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHH
REKKNDICLEDIVSCGSGGVLCVEAGGPKPGSGCAGRGIITAFEQLEELGAYTTYHPDII
HHCCCCCCHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCEE
LYDVLGDVVCGGFALPIRQGYADEICIVTSGEMMSLYAADNISQAVNNYSKRGYAALKGL
HHHHHHHHHHCCCHHHHHCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
IYNAKNIENEDEIVEKAAAEMNTKVLSRINRDPIVQKAENAGMTVVEYAPNSSQADLYRK
HHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCCCCHHHHHHH
LAGILLED
HHHHHCCC
>Mature Secondary Structure
MKKIAFYGKGGIGKSTCVSNISMALAEQGFTVMQIGCDPKADSTRNLTHGDLIPSVLSVI
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHH
REKKNDICLEDIVSCGSGGVLCVEAGGPKPGSGCAGRGIITAFEQLEELGAYTTYHPDII
HHCCCCCCHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCEE
LYDVLGDVVCGGFALPIRQGYADEICIVTSGEMMSLYAADNISQAVNNYSKRGYAALKGL
HHHHHHHHHHCCCHHHHHCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
IYNAKNIENEDEIVEKAAAEMNTKVLSRINRDPIVQKAENAGMTVVEYAPNSSQADLYRK
HHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCCCCHHHHHHH
LAGILLED
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA