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The map label for this gene is glnA [H]

Identifier: 124485688

GI number: 124485688

Start: 856218

End: 857720

Strand: Reverse

Name: glnA [H]

Synonym: Mlab_0866

Alternate gene names: 124485688

Gene position: 857720-856218 (Counterclockwise)

Preceding gene: 124485692

Following gene: 124485687

Centisome position: 47.52

GC content: 52.1

Gene sequence:

>1503_bases
ATGAATGATGCGGAGGTCCTGATGAACCCAAACGACTTAGTGCAGTTTTTGAAAAAAAATCCTGAGTATTTTACAAAAGA
TGACATCCTCCGTTTTTGCGAAGAAAACCATGTTGAGATGGTTAACTTCCGCTATGCCGGCGGCGACGGGAAACTCAAAA
CCCTGAACTTCGCCGTATCGTCAAAAGAGCAGCTTGATACGATCCTCTCCGAAGGCGAACGGGTCGACGGCAGCAAACTT
TTCTCTTTTATCGAGGCAGACAGTTCCGATCTCTACGTTATCCCAAGATACGGGACGGCGTTCATGGATCCTTTTGCAGA
GGCCAAAACCCTGAACATCCTCTGTTCCTATTACGACGGGAACGGGCATCCGCTTGCCTCCTCGCCGGAACACATCCTTC
GCCAGGCAGAAGCATCATTCAAAAAACACACCGGGATGGAGTTCAAATGCCTCGGCGAACTTGAATACTATATCATCTCC
AAAGAGGAGACCTTCTATCACCGAAGCAACAGGATCGGGTATCACGAATCGGGCCCCTTCTGCAGATTCTGCGAGATGCG
GACCGAAGCCATGCTTATGATCGCCCGTGCCGGCGGGAAGATCAAATACGGCCATGCCGAAGCGGGTTCTTTTACAAAAG
ACGGCTATTATTACGAGCAGCACGAGATCGAGTTCCTGCTGACAAGCCCGATCCTCGCCGTCGAACAGATCCTCATTGCA
AAATGGATTCTCAGAAGACTCAGCGTTAAGTACGAGGTCGAGGTCTCGTTTGCGCCGAAGATCGCCATAGACAGATCGGG
TTCCGGTCTGCATATCCACCTCCTTGCTGAGAAAAACGGCACGAACGTACTGATCAAAGAGGGAAAACTTTCCGACACGG
CAAAGCGGATGATCGTCGGGATTCTGGACATCAGCGATGCGGCTACCGGATTTGGGAACACCGTTCCTACTTCATATCTG
AGACTTGTGCCGAACCAAAACGCACCGACCTACATCTGCTGGGGAGAACAGAACCGATCGGTCGTGGTCCGCGTCCCGCT
TGGCTGGGTCGACGGAGTCGATATGGCGGCTGATGCAAACTTCGGCCTTAAGAGAAGATCCGAAAAAGCTACCAGACAGA
CCCTGGAATTCAGGGTCGCCGACGGTTCGGCCTGCATCTACGAAATGATCGCCTGTTTGATCTGCGGGGCGATCCACGGT
CTCGGCATGAACGGTGCCCTCACCAAAGCAGACGAACTCTATGCATCGAGAAATATCTTCAATGAGGAGTGCACGTCGTT
CCTTAAAACTCTGCGCCGGCTGCCGATCAGCTGCACAGAATCGGCCGATGCGCTGATTTCCAAACGAAAACTGCTCGAAA
GAGACGACATCTTCCCGCCGGGAGTCATCGACGACCAGGCGGCAAAACTGAGAGCGTATAATGACAGTTGTCTTTCTGAA
AGACTTCGCAGGGACGAGACCCTCTTCACGGAATGTGCGGAGCAGTATCTCCACATCAACTGA

Upstream 100 bases:

>100_bases
CCGGGCTGTTTTTGCCATCGGACGAAATATGAAAAAAACCGGTTCGATACTTAAAAAACCCAGTCTCCTTAAGTACCAAA
AGAACCCATATTAATATATT

Downstream 100 bases:

>100_bases
TCTGACATGGGATTTTTAGGCAATATCGAACGGGCACTCGGTGTGTGGTATCCGGACAAAGACACGGAACCCGATGACGG
CAACCCTCTGGTAGCTCTGG

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MNDAEVLMNPNDLVQFLKKNPEYFTKDDILRFCEENHVEMVNFRYAGGDGKLKTLNFAVSSKEQLDTILSEGERVDGSKL
FSFIEADSSDLYVIPRYGTAFMDPFAEAKTLNILCSYYDGNGHPLASSPEHILRQAEASFKKHTGMEFKCLGELEYYIIS
KEETFYHRSNRIGYHESGPFCRFCEMRTEAMLMIARAGGKIKYGHAEAGSFTKDGYYYEQHEIEFLLTSPILAVEQILIA
KWILRRLSVKYEVEVSFAPKIAIDRSGSGLHIHLLAEKNGTNVLIKEGKLSDTAKRMIVGILDISDAATGFGNTVPTSYL
RLVPNQNAPTYICWGEQNRSVVVRVPLGWVDGVDMAADANFGLKRRSEKATRQTLEFRVADGSACIYEMIACLICGAIHG
LGMNGALTKADELYASRNIFNEECTSFLKTLRRLPISCTESADALISKRKLLERDDIFPPGVIDDQAAKLRAYNDSCLSE
RLRRDETLFTECAEQYLHIN

Sequences:

>Translated_500_residues
MNDAEVLMNPNDLVQFLKKNPEYFTKDDILRFCEENHVEMVNFRYAGGDGKLKTLNFAVSSKEQLDTILSEGERVDGSKL
FSFIEADSSDLYVIPRYGTAFMDPFAEAKTLNILCSYYDGNGHPLASSPEHILRQAEASFKKHTGMEFKCLGELEYYIIS
KEETFYHRSNRIGYHESGPFCRFCEMRTEAMLMIARAGGKIKYGHAEAGSFTKDGYYYEQHEIEFLLTSPILAVEQILIA
KWILRRLSVKYEVEVSFAPKIAIDRSGSGLHIHLLAEKNGTNVLIKEGKLSDTAKRMIVGILDISDAATGFGNTVPTSYL
RLVPNQNAPTYICWGEQNRSVVVRVPLGWVDGVDMAADANFGLKRRSEKATRQTLEFRVADGSACIYEMIACLICGAIHG
LGMNGALTKADELYASRNIFNEECTSFLKTLRRLPISCTESADALISKRKLLERDDIFPPGVIDDQAAKLRAYNDSCLSE
RLRRDETLFTECAEQYLHIN
>Mature_500_residues
MNDAEVLMNPNDLVQFLKKNPEYFTKDDILRFCEENHVEMVNFRYAGGDGKLKTLNFAVSSKEQLDTILSEGERVDGSKL
FSFIEADSSDLYVIPRYGTAFMDPFAEAKTLNILCSYYDGNGHPLASSPEHILRQAEASFKKHTGMEFKCLGELEYYIIS
KEETFYHRSNRIGYHESGPFCRFCEMRTEAMLMIARAGGKIKYGHAEAGSFTKDGYYYEQHEIEFLLTSPILAVEQILIA
KWILRRLSVKYEVEVSFAPKIAIDRSGSGLHIHLLAEKNGTNVLIKEGKLSDTAKRMIVGILDISDAATGFGNTVPTSYL
RLVPNQNAPTYICWGEQNRSVVVRVPLGWVDGVDMAADANFGLKRRSEKATRQTLEFRVADGSACIYEMIACLICGAIHG
LGMNGALTKADELYASRNIFNEECTSFLKTLRRLPISCTESADALISKRKLLERDDIFPPGVIDDQAAKLRAYNDSCLSE
RLRRDETLFTECAEQYLHIN

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=412, Percent_Identity=23.5436893203883, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI87081870, Length=308, Percent_Identity=24.6753246753247, Blast_Score=86, Evalue=7e-18,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 56317; Mature: 56317

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDAEVLMNPNDLVQFLKKNPEYFTKDDILRFCEENHVEMVNFRYAGGDGKLKTLNFAVS
CCCCCEEECHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEEEEC
SKEQLDTILSEGERVDGSKLFSFIEADSSDLYVIPRYGTAFMDPFAEAKTLNILCSYYDG
CHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEEECCCCHHHCHHHCCEEEEEEEEEECC
NGHPLASSPEHILRQAEASFKKHTGMEFKCLGELEYYIISKEETFYHRSNRIGYHESGPF
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCEEEEEEECCHHHHHHCCCCCCCCCCCC
CRFCEMRTEAMLMIARAGGKIKYGHAEAGSFTKDGYYYEQHEIEFLLTSPILAVEQILIA
HHHHHHHHHHEEEEEECCCEEEECCCCCCCCCCCCEEEECCCEEEEECCHHHHHHHHHHH
KWILRRLSVKYEVEVSFAPKIAIDRSGSGLHIHLLAEKNGTNVLIKEGKLSDTAKRMIVG
HHHHHHCCEEEEEEEEECCEEEEECCCCCEEEEEEEECCCCEEEEECCCCCHHHHHHEEE
ILDISDAATGFGNTVPTSYLRLVPNQNAPTYICWGEQNRSVVVRVPLGWVDGVDMAADAN
EEECCCCCCCCCCCCCCHHEEECCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCC
FGLKRRSEKATRQTLEFRVADGSACIYEMIACLICGAIHGLGMNGALTKADELYASRNIF
CCCHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCC
NEECTSFLKTLRRLPISCTESADALISKRKLLERDDIFPPGVIDDQAAKLRAYNDSCLSE
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHHHH
RLRRDETLFTECAEQYLHIN
HHHCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNDAEVLMNPNDLVQFLKKNPEYFTKDDILRFCEENHVEMVNFRYAGGDGKLKTLNFAVS
CCCCCEEECHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEEEEC
SKEQLDTILSEGERVDGSKLFSFIEADSSDLYVIPRYGTAFMDPFAEAKTLNILCSYYDG
CHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEEECCCCHHHCHHHCCEEEEEEEEEECC
NGHPLASSPEHILRQAEASFKKHTGMEFKCLGELEYYIISKEETFYHRSNRIGYHESGPF
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCEEEEEEECCHHHHHHCCCCCCCCCCCC
CRFCEMRTEAMLMIARAGGKIKYGHAEAGSFTKDGYYYEQHEIEFLLTSPILAVEQILIA
HHHHHHHHHHEEEEEECCCEEEECCCCCCCCCCCCEEEECCCEEEEECCHHHHHHHHHHH
KWILRRLSVKYEVEVSFAPKIAIDRSGSGLHIHLLAEKNGTNVLIKEGKLSDTAKRMIVG
HHHHHHCCEEEEEEEEECCEEEEECCCCCEEEEEEEECCCCEEEEECCCCCHHHHHHEEE
ILDISDAATGFGNTVPTSYLRLVPNQNAPTYICWGEQNRSVVVRVPLGWVDGVDMAADAN
EEECCCCCCCCCCCCCCHHEEECCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCC
FGLKRRSEKATRQTLEFRVADGSACIYEMIACLICGAIHGLGMNGALTKADELYASRNIF
CCCHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCC
NEECTSFLKTLRRLPISCTESADALISKRKLLERDDIFPPGVIDDQAAKLRAYNDSCLSE
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHHHH
RLRRDETLFTECAEQYLHIN
HHHCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2572584 [H]