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The map label for this gene is sepS

Identifier: 124485362

GI number: 124485362

Start: 509936

End: 511516

Strand: Reverse

Name: sepS

Synonym: Mlab_0537

Alternate gene names: 124485362

Gene position: 511516-509936 (Counterclockwise)

Preceding gene: 124485363

Following gene: 124485359

Centisome position: 28.34

GC content: 55.41

Gene sequence:

>1581_bases
ATGAAATTCGATATTGAGGAATTCAAGGAACGCAGAAAAACCGATTTCGAGGGTGCCTGGCATGCCGGCCCATCGGTCAT
AACCCCGCCGGAATCATCAAAAATTTATCCGCGTTATGCGTATCGCCGAGCAAAAGTGCACCCGATCTTCGACACGATCG
CCCGTCTTAGGGCCGCCTATATGTCGATGGGATTCGATGAGGCAATGGTACCGGTGTTTATCGACGAACAGGACGTCTAC
CGTCAGTTCGGTCCGGAAGCCGCCGCGGTTTTGGATCGCGTCTACTATGTGGGCGGCCTTCCCCGTCCGAATGTAGGGAT
ATCCCGGGAACGGCTGGATGCAATCGCCGAAATCATCGAAAAGCCTCTCGCCGAAGGCACGGAAGAGAAGCTGATGAAGT
GTCTGCATGCCTACAAGAAAGGCAAATTCGACGGTGACGATCTCACGCACGAAATGTCGGTCGCTTTAGGCGTGGACGAT
GGTGTGGTCGTTCACATTCTCGATACGGTCTTTCCCGAATTCAAGGAGCTCGCCCCTGAGTCTTCCAGAACGACGCTTCG
TTCCCATATGACCAGCGGCTGGTTCATCTCTCTTGCCCAGATGTGGGATAAAAAGCCGATGCCGATTCGAATGTTTTCGG
TCGACCGGTGTTTCCGCCGCGAGCAGGAGGAGGATGCGACCCATCTTCGGACCTATCACTCGGCATCCTGTATCGTCGCC
GGAGAAGATGTGACGGTCGAAGAAGGAAAGGCGGTCGCCGAAGGTCTTCTTTCCGCGTTCGGTTTCACCGAGTTCAGATT
CCAGCCCGACGAGAAACGCTCGAAGTACTACATGCCCGAGACGCAGACCGAAGTATACGGCAAACACCCGGTCCACGGAT
GGGTCGAGGTCGCTACGTTCGGGATCTACTCACCGGCAGCCCTCGCCGAATACGGCGTAGGCGTTCCCGTTATGAACCTC
GGCATGGGTGTCGAGCGTCTTGCGATGGTCTTAACCCAGGCGGAGGATGTTAGGAAACTCTCGTTTGCCCAACTGTATCC
TCCGGTGTATTCGGACACGGATCTTACCAAAGGCATCGGATTAAGAGAGGAGCCGCAGACGGCCGAGGGCCGCCGGGCAG
TGCGTGCTGTGATGGAAACGGCCGCTGCCCATGCGGCTGAACGTTCGCCGTGTTCGTTCCCTGCATGGAAAGGCGAGCTC
TACGGTCATCAGGTGGAGATCGTCGTTGAGGAGCCCGAAGAGAATACGAGTCTTCTCGGTCCTGCCGCTTTGAATGAGGT
ATATGTCAGAAAAGGAGCAGTTCTCGGTGTTCCGGATACCGAAAAGTTCGCCGACGTCAAAGCCGAAGGGGTTCCGGTTG
GAATCTCTTTCCTTTACTCGACGGCAAATCTGGCACTCGCCCGCATCGAGGAGGCGGCGAGGGTCGGCGAAGGGACGAGC
ATTCAGGTGAAAATGTCGAAGCATCCAAGCGACGTGAACCTGAAAATCGAGGAGTACGTTATGCGGTACATCACCGACAA
TAAAAAGAAACTCGATCTTCGCGGCCCGGTCTTTATGACGATTACGTCGAAGATCCTGTAA

Upstream 100 bases:

>100_bases
AAACCATTCGTCGTTTCGCCGCAGGAGTTGCCCAACTCTGCAATTATGAGATTATGGATGAAAACGTGCCGAGCCGCGTT
GTTTGTCTGAAGAGAAAATC

Downstream 100 bases:

>100_bases
AATACTTTTATCCTTATTTTTTGAGTTTTATCTGGTTCAACTTCGGTAGGATCATTTTTTTTCGAGATCTCTGAGAAGAA
CGGCAAAGTCGATCACGAAC

Product: O-phosphoseryl-tRNA synthetase

Products: NA

Alternate protein names: SepRS; Non-canonical O-phosphoseryl-tRNA(Cys) synthetase; O-phosphoserine--tRNA(Cys) ligase; O-phosphoserine--tRNA ligase

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MKFDIEEFKERRKTDFEGAWHAGPSVITPPESSKIYPRYAYRRAKVHPIFDTIARLRAAYMSMGFDEAMVPVFIDEQDVY
RQFGPEAAAVLDRVYYVGGLPRPNVGISRERLDAIAEIIEKPLAEGTEEKLMKCLHAYKKGKFDGDDLTHEMSVALGVDD
GVVVHILDTVFPEFKELAPESSRTTLRSHMTSGWFISLAQMWDKKPMPIRMFSVDRCFRREQEEDATHLRTYHSASCIVA
GEDVTVEEGKAVAEGLLSAFGFTEFRFQPDEKRSKYYMPETQTEVYGKHPVHGWVEVATFGIYSPAALAEYGVGVPVMNL
GMGVERLAMVLTQAEDVRKLSFAQLYPPVYSDTDLTKGIGLREEPQTAEGRRAVRAVMETAAAHAAERSPCSFPAWKGEL
YGHQVEIVVEEPEENTSLLGPAALNEVYVRKGAVLGVPDTEKFADVKAEGVPVGISFLYSTANLALARIEEAARVGEGTS
IQVKMSKHPSDVNLKIEEYVMRYITDNKKKLDLRGPVFMTITSKIL

Sequences:

>Translated_526_residues
MKFDIEEFKERRKTDFEGAWHAGPSVITPPESSKIYPRYAYRRAKVHPIFDTIARLRAAYMSMGFDEAMVPVFIDEQDVY
RQFGPEAAAVLDRVYYVGGLPRPNVGISRERLDAIAEIIEKPLAEGTEEKLMKCLHAYKKGKFDGDDLTHEMSVALGVDD
GVVVHILDTVFPEFKELAPESSRTTLRSHMTSGWFISLAQMWDKKPMPIRMFSVDRCFRREQEEDATHLRTYHSASCIVA
GEDVTVEEGKAVAEGLLSAFGFTEFRFQPDEKRSKYYMPETQTEVYGKHPVHGWVEVATFGIYSPAALAEYGVGVPVMNL
GMGVERLAMVLTQAEDVRKLSFAQLYPPVYSDTDLTKGIGLREEPQTAEGRRAVRAVMETAAAHAAERSPCSFPAWKGEL
YGHQVEIVVEEPEENTSLLGPAALNEVYVRKGAVLGVPDTEKFADVKAEGVPVGISFLYSTANLALARIEEAARVGEGTS
IQVKMSKHPSDVNLKIEEYVMRYITDNKKKLDLRGPVFMTITSKIL
>Mature_526_residues
MKFDIEEFKERRKTDFEGAWHAGPSVITPPESSKIYPRYAYRRAKVHPIFDTIARLRAAYMSMGFDEAMVPVFIDEQDVY
RQFGPEAAAVLDRVYYVGGLPRPNVGISRERLDAIAEIIEKPLAEGTEEKLMKCLHAYKKGKFDGDDLTHEMSVALGVDD
GVVVHILDTVFPEFKELAPESSRTTLRSHMTSGWFISLAQMWDKKPMPIRMFSVDRCFRREQEEDATHLRTYHSASCIVA
GEDVTVEEGKAVAEGLLSAFGFTEFRFQPDEKRSKYYMPETQTEVYGKHPVHGWVEVATFGIYSPAALAEYGVGVPVMNL
GMGVERLAMVLTQAEDVRKLSFAQLYPPVYSDTDLTKGIGLREEPQTAEGRRAVRAVMETAAAHAAERSPCSFPAWKGEL
YGHQVEIVVEEPEENTSLLGPAALNEVYVRKGAVLGVPDTEKFADVKAEGVPVGISFLYSTANLALARIEEAARVGEGTS
IQVKMSKHPSDVNLKIEEYVMRYITDNKKKLDLRGPVFMTITSKIL

Specific function: Catalyzes the attachment of O-phosphoserine (Sep) to tRNA(Cys)

COG id: COG2024

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit (archaeal type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. O-phosphoseryl-tRNA(Cys) synthetase subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=167, Percent_Identity=32.9341317365269, Blast_Score=84, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI32563657, Length=166, Percent_Identity=28.3132530120482, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17508957, Length=166, Percent_Identity=28.3132530120482, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321087, Length=190, Percent_Identity=30, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SEPS_METLZ (A2SQV5)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001029978.1
- ProteinModelPortal:   A2SQV5
- SMR:   A2SQV5
- STRING:   A2SQV5
- GeneID:   4795209
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_0537
- eggNOG:   arNOG04874
- HOGENOM:   HBG539012
- OMA:   TLRSHMT
- ProtClustDB:   PRK06253
- BioCyc:   MLAB410358:MLAB_0537-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01674
- InterPro:   IPR006195
- InterPro:   IPR005246
- InterPro:   IPR002319
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00470

Pfam domain/function: PF01409 tRNA-synt_2d

EC number: =6.1.1.27

Molecular weight: Translated: 58871; Mature: 58871

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: BINDING 319-319

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFDIEEFKERRKTDFEGAWHAGPSVITPPESSKIYPRYAYRRAKVHPIFDTIARLRAAY
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHH
MSMGFDEAMVPVFIDEQDVYRQFGPEAAAVLDRVYYVGGLPRPNVGISRERLDAIAEIIE
HHCCCCCCCCEEEECCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
KPLAEGTEEKLMKCLHAYKKGKFDGDDLTHEMSVALGVDDGVVVHILDTVFPEFKELAPE
HHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCC
SSRTTLRSHMTSGWFISLAQMWDKKPMPIRMFSVDRCFRREQEEDATHLRTYHSASCIVA
CHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEE
GEDVTVEEGKAVAEGLLSAFGFTEFRFQPDEKRSKYYMPETQTEVYGKHPVHGWVEVATF
CCCCCHHCCHHHHHHHHHHHCCCCEEECCCHHCCCCCCCCCCHHHCCCCCCCHHHHHHHH
GIYSPAALAEYGVGVPVMNLGMGVERLAMVLTQAEDVRKLSFAQLYPPVYSDTDLTKGIG
CCCCCHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCC
LREEPQTAEGRRAVRAVMETAAAHAAERSPCSFPAWKGELYGHQVEIVVEEPEENTSLLG
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEC
PAALNEVYVRKGAVLGVPDTEKFADVKAEGVPVGISFLYSTANLALARIEEAARVGEGTS
HHHHHHHHHHCCCEECCCCCHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCE
IQVKMSKHPSDVNLKIEEYVMRYITDNKKKLDLRGPVFMTITSKIL
EEEEECCCCCCCCEEHHHHHHHHHCCCCCEEECCCCEEEEEHHHCC
>Mature Secondary Structure
MKFDIEEFKERRKTDFEGAWHAGPSVITPPESSKIYPRYAYRRAKVHPIFDTIARLRAAY
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHH
MSMGFDEAMVPVFIDEQDVYRQFGPEAAAVLDRVYYVGGLPRPNVGISRERLDAIAEIIE
HHCCCCCCCCEEEECCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
KPLAEGTEEKLMKCLHAYKKGKFDGDDLTHEMSVALGVDDGVVVHILDTVFPEFKELAPE
HHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCC
SSRTTLRSHMTSGWFISLAQMWDKKPMPIRMFSVDRCFRREQEEDATHLRTYHSASCIVA
CHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEE
GEDVTVEEGKAVAEGLLSAFGFTEFRFQPDEKRSKYYMPETQTEVYGKHPVHGWVEVATF
CCCCCHHCCHHHHHHHHHHHCCCCEEECCCHHCCCCCCCCCCHHHCCCCCCCHHHHHHHH
GIYSPAALAEYGVGVPVMNLGMGVERLAMVLTQAEDVRKLSFAQLYPPVYSDTDLTKGIG
CCCCCHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCC
LREEPQTAEGRRAVRAVMETAAAHAAERSPCSFPAWKGELYGHQVEIVVEEPEENTSLLG
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEC
PAALNEVYVRKGAVLGVPDTEKFADVKAEGVPVGISFLYSTANLALARIEEAARVGEGTS
HHHHHHHHHHCCCEECCCCCHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCE
IQVKMSKHPSDVNLKIEEYVMRYITDNKKKLDLRGPVFMTITSKIL
EEEEECCCCCCCCEEHHHHHHHHHCCCCCEEECCCCEEEEEHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA