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The map label for this gene is dapA

Identifier: 124485355

GI number: 124485355

Start: 503772

End: 504665

Strand: Reverse

Name: dapA

Synonym: Mlab_0530

Alternate gene names: 124485355

Gene position: 504665-503772 (Counterclockwise)

Preceding gene: 124485358

Following gene: 124485354

Centisome position: 27.96

GC content: 54.81

Gene sequence:

>894_bases
ATGTTTGAAGGTGTAATCCCTGCACTCATCACACCGTTCAATGACGACGCGTCCCAGTCGCTCGATATTGAAGGTCTGAG
AAACTGCATCGAGTATGTCATACGCGGAGGCGTTCACGGACTTCTCGCATGCGGTTCGACAGGAGAATCGGCAACAATGA
CACACGCCGAGCACATTCGTGTCATTGAGGAAACCGTTTCTGCTGCGAGCAACCGCATTCCTGCCATCGCAGGAACCGGT
TCGAACAATACGGAAGAAGCTCTTCTGATGACCCGTGCTGCAAAGATGTCCGGAGCAGACGGTGTACTTCTGATCAGCCC
GTATTACAACAAGCCGAACAGATCGGGTCTGCTGAAGCATTACCGCAAGGTCGCCGACATCGGTCTTCCGGTCATTGTGT
ACAACATCCCGGGACGTACCGGTCAGAACCTGCCTGCCGATCTGATCATCGAGATGGTGGAGACGATTCCAAATCTGGTC
GCCGTTAAGGAAGCCAGCGGAAATCTCGACCAGATGATGGCGATCATCCAGGGCACCCAGGATCTCGACCGCGAGTATCC
GTTCATCCTTACGTCAGGCGACGACTCGCTGACGCTTCCGGTCCTTTCGGTCGGCGGCCACGGCTGTATTTCCGTTACGG
CAAACATCGAGCCAAAGCGCTTGGTCGAGATGTACGATCTCTTTAGAAGATCCGACGTCAAGGCGGCGATGAAGAAGCAT
TACGAGCTGATGGATCTGTCCAAGGCGCTCTTTATGGAAGTGAACCCGGTGCCGGTCAAGCGTGCCGCAGAGATCCGCGG
TCTGATCAACTCAGGCAACGTCAGACTTCCATTGGATTCGCTTGGCGAGCAGAACACGGCGATCCTTCGCGGGGTGCTTT
CCCGCTATGATTAA

Upstream 100 bases:

>100_bases
CTTTTCGTGCAGGGGGCAGGCACAAGATGCGTGTGTCCTAAAAGAGACTGCTCTGACTGCGCAAAATACATCATCTCGTT
CAACCAAATGGTAATGTACT

Downstream 100 bases:

>100_bases
GGTCATCATCTGCGGCGCACTCGGCAGGATGGGAACCATGATCGCGAACATGGTGATCGAGAACCCGGAGCTGGAGTTCG
TCGGCGGCGTGGATATCCGG

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MFEGVIPALITPFNDDASQSLDIEGLRNCIEYVIRGGVHGLLACGSTGESATMTHAEHIRVIEETVSAASNRIPAIAGTG
SNNTEEALLMTRAAKMSGADGVLLISPYYNKPNRSGLLKHYRKVADIGLPVIVYNIPGRTGQNLPADLIIEMVETIPNLV
AVKEASGNLDQMMAIIQGTQDLDREYPFILTSGDDSLTLPVLSVGGHGCISVTANIEPKRLVEMYDLFRRSDVKAAMKKH
YELMDLSKALFMEVNPVPVKRAAEIRGLINSGNVRLPLDSLGEQNTAILRGVLSRYD

Sequences:

>Translated_297_residues
MFEGVIPALITPFNDDASQSLDIEGLRNCIEYVIRGGVHGLLACGSTGESATMTHAEHIRVIEETVSAASNRIPAIAGTG
SNNTEEALLMTRAAKMSGADGVLLISPYYNKPNRSGLLKHYRKVADIGLPVIVYNIPGRTGQNLPADLIIEMVETIPNLV
AVKEASGNLDQMMAIIQGTQDLDREYPFILTSGDDSLTLPVLSVGGHGCISVTANIEPKRLVEMYDLFRRSDVKAAMKKH
YELMDLSKALFMEVNPVPVKRAAEIRGLINSGNVRLPLDSLGEQNTAILRGVLSRYD
>Mature_297_residues
MFEGVIPALITPFNDDASQSLDIEGLRNCIEYVIRGGVHGLLACGSTGESATMTHAEHIRVIEETVSAASNRIPAIAGTG
SNNTEEALLMTRAAKMSGADGVLLISPYYNKPNRSGLLKHYRKVADIGLPVIVYNIPGRTGQNLPADLIIEMVETIPNLV
AVKEASGNLDQMMAIIQGTQDLDREYPFILTSGDDSLTLPVLSVGGHGCISVTANIEPKRLVEMYDLFRRSDVKAAMKKH
YELMDLSKALFMEVNPVPVKRAAEIRGLINSGNVRLPLDSLGEQNTAILRGVLSRYD

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=290, Percent_Identity=25.1724137931034, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI13540533, Length=245, Percent_Identity=24.8979591836735, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI1788823, Length=296, Percent_Identity=36.8243243243243, Blast_Score=197, Evalue=5e-52,
Organism=Escherichia coli, GI87082415, Length=269, Percent_Identity=30.1115241635688, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1786463, Length=290, Percent_Identity=29.6551724137931, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1789620, Length=242, Percent_Identity=34.297520661157, Blast_Score=123, Evalue=1e-29,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_METLZ (A2SQU8)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001029971.1
- ProteinModelPortal:   A2SQU8
- SMR:   A2SQU8
- STRING:   A2SQU8
- GeneID:   4795410
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_0530
- eggNOG:   arNOG05039
- HOGENOM:   HBG358848
- OMA:   PAIITPF
- PhylomeDB:   A2SQU8
- BioCyc:   MLAB410358:MLAB_0530-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 32189; Mature: 32189

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 163-163 BINDING 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEGVIPALITPFNDDASQSLDIEGLRNCIEYVIRGGVHGLLACGSTGESATMTHAEHIR
CCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
VIEETVSAASNRIPAIAGTGSNNTEEALLMTRAAKMSGADGVLLISPYYNKPNRSGLLKH
HHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHH
YRKVADIGLPVIVYNIPGRTGQNLPADLIIEMVETIPNLVAVKEASGNLDQMMAIIQGTQ
HHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCHHEEEECCCCCHHHHHHHHHCCH
DLDREYPFILTSGDDSLTLPVLSVGGHGCISVTANIEPKRLVEMYDLFRRSDVKAAMKKH
HHCCCCCEEEECCCCCEEEEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
YELMDLSKALFMEVNPVPVKRAAEIRGLINSGNVRLPLDSLGEQNTAILRGVLSRYD
HHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCEEECHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MFEGVIPALITPFNDDASQSLDIEGLRNCIEYVIRGGVHGLLACGSTGESATMTHAEHIR
CCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
VIEETVSAASNRIPAIAGTGSNNTEEALLMTRAAKMSGADGVLLISPYYNKPNRSGLLKH
HHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHH
YRKVADIGLPVIVYNIPGRTGQNLPADLIIEMVETIPNLVAVKEASGNLDQMMAIIQGTQ
HHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCHHEEEECCCCCHHHHHHHHHCCH
DLDREYPFILTSGDDSLTLPVLSVGGHGCISVTANIEPKRLVEMYDLFRRSDVKAAMKKH
HHCCCCCEEEECCCCCEEEEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
YELMDLSKALFMEVNPVPVKRAAEIRGLINSGNVRLPLDSLGEQNTAILRGVLSRYD
HHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCEEECHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA