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The map label for this gene is speB [C]

Identifier: 124485252

GI number: 124485252

Start: 410468

End: 411319

Strand: Reverse

Name: speB [C]

Synonym: Mlab_0425

Alternate gene names: 124485252

Gene position: 411319-410468 (Counterclockwise)

Preceding gene: 124485253

Following gene: 124485250

Centisome position: 22.79

GC content: 48.59

Gene sequence:

>852_bases
ATGCAGTCTTTCTCTAACACTCTTTTTGCTGATGCTGAAAGCGAATATTCAGATGCATCATATGGGATATTTGGCGTTCC
CTTCGATGGGACAACCTCTTTTAAAGCAGGATGCAGAGATGCGCCTGCAGCGATACGTCAGGCATCATACAATATCGAGA
CGTACAATCCCTATTACGACATAGAGCTTCCCGACATTCCGTTTCATGATATGGGCGATATTTATGCAGAATGTCTGCCC
GACCTCGTTGTCGCCCAGGTTGAGGATGTTGTTCTCGATCTGATGAAAGACGGGAAGATCCCGATAATGATTGGTGGAGA
ACATTCGATAACGATCGGTGCCGTCAGAGCTCTTTCTCCATCCTGGTATGTTGTCTGTGATGCCCACCTTGATATGCAGG
ATGAATACCGAGGATCCCCTTATAATCACGACTGCGTAACCGCACGCGTGAGCGAGATCACGCCAAATATCATCATTCTT
GGGGCACGAAGCGGATCACGAGACGAGTTCTTACTTGCCAAGGAGAAGTATCATCTGTATACGGCTGATGATATCAAGGA
TCGGGGCATTGGGACAGTGTTGAATGAGATTAAAGCGCTGATAGGGGATGCAGATCTGTATCTTTCTATCGACGCGGATT
CGATTGACTGCTGCCTGACTCCCGGCCTTGGCACACCGGAACCATTCGGGCTTACTCCAAGAGAGGTGCGGGAGGTTGTA
AGAGCTCTTGCCAAAAATGCCGTTGGATTTGATTATGTGGAGGTGCTTCCAAACGATGCTGGTCAAACCGCGATGGTTGC
AGCCCATATCATCCGCGAGTTCATTGCAGGTCACGCCTGCAAATCTAAATGA

Upstream 100 bases:

>100_bases
GAACACATTCTGGCAACCTTCCAGCCGGCTCAGGAAGTTCCCTACATCGAGTCACTCGGCAAGCGTAAGCTTGACCTCTA
AGACTGCACAATAATCCTAT

Downstream 100 bases:

>100_bases
ACCTTGGTTTCATCCCTAACCATCATCAATTTGCTTTTTTGTAGGTGAATGCATTTAGCCTTAAAACGCAGCTTACAGCT
AAAAAAGGAGGGAGTATTTA

Product: agmatinase

Products: putrescine; urea

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MQSFSNTLFADAESEYSDASYGIFGVPFDGTTSFKAGCRDAPAAIRQASYNIETYNPYYDIELPDIPFHDMGDIYAECLP
DLVVAQVEDVVLDLMKDGKIPIMIGGEHSITIGAVRALSPSWYVVCDAHLDMQDEYRGSPYNHDCVTARVSEITPNIIIL
GARSGSRDEFLLAKEKYHLYTADDIKDRGIGTVLNEIKALIGDADLYLSIDADSIDCCLTPGLGTPEPFGLTPREVREVV
RALAKNAVGFDYVEVLPNDAGQTAMVAAHIIREFIAGHACKSK

Sequences:

>Translated_283_residues
MQSFSNTLFADAESEYSDASYGIFGVPFDGTTSFKAGCRDAPAAIRQASYNIETYNPYYDIELPDIPFHDMGDIYAECLP
DLVVAQVEDVVLDLMKDGKIPIMIGGEHSITIGAVRALSPSWYVVCDAHLDMQDEYRGSPYNHDCVTARVSEITPNIIIL
GARSGSRDEFLLAKEKYHLYTADDIKDRGIGTVLNEIKALIGDADLYLSIDADSIDCCLTPGLGTPEPFGLTPREVREVV
RALAKNAVGFDYVEVLPNDAGQTAMVAAHIIREFIAGHACKSK
>Mature_283_residues
MQSFSNTLFADAESEYSDASYGIFGVPFDGTTSFKAGCRDAPAAIRQASYNIETYNPYYDIELPDIPFHDMGDIYAECLP
DLVVAQVEDVVLDLMKDGKIPIMIGGEHSITIGAVRALSPSWYVVCDAHLDMQDEYRGSPYNHDCVTARVSEITPNIIIL
GARSGSRDEFLLAKEKYHLYTADDIKDRGIGTVLNEIKALIGDADLYLSIDADSIDCCLTPGLGTPEPFGLTPREVREVV
RALAKNAVGFDYVEVLPNDAGQTAMVAAHIIREFIAGHACKSK

Specific function: Catalyzes The Formation Of Putrescine From Agmatine. [C]

COG id: COG0010

COG function: function code E; Arginase/agmatinase/formimionoglutamate hydrolase, arginase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the arginase family [H]

Homologues:

Organism=Homo sapiens, GI37537722, Length=291, Percent_Identity=28.8659793814433, Blast_Score=104, Evalue=7e-23,
Organism=Escherichia coli, GI1789306, Length=251, Percent_Identity=32.2709163346614, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005925
- InterPro:   IPR006035
- InterPro:   IPR020855 [H]

Pfam domain/function: PF00491 Arginase [H]

EC number: 3.5.3.11

Molecular weight: Translated: 30928; Mature: 30928

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS00147 ARGINASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSFSNTLFADAESEYSDASYGIFGVPFDGTTSFKAGCRDAPAAIRQASYNIETYNPYYD
CCCCCCCEEECCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHCCCCEECCCEEE
IELPDIPFHDMGDIYAECLPDLVVAQVEDVVLDLMKDGKIPIMIGGEHSITIGAVRALSP
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEHEECCC
SWYVVCDAHLDMQDEYRGSPYNHDCVTARVSEITPNIIILGARSGSRDEFLLAKEKYHLY
CEEEEEECCCCCHHHHCCCCCCCCHHEEEHHHCCCCEEEEECCCCCCCCEEEEECEEEEE
TADDIKDRGIGTVLNEIKALIGDADLYLSIDADSIDCCLTPGLGTPEPFGLTPREVREVV
ECCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCCCCCCCCCCCHHHHHHHH
RALAKNAVGFDYVEVLPNDAGQTAMVAAHIIREFIAGHACKSK
HHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQSFSNTLFADAESEYSDASYGIFGVPFDGTTSFKAGCRDAPAAIRQASYNIETYNPYYD
CCCCCCCEEECCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHCCCCEECCCEEE
IELPDIPFHDMGDIYAECLPDLVVAQVEDVVLDLMKDGKIPIMIGGEHSITIGAVRALSP
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEHEECCC
SWYVVCDAHLDMQDEYRGSPYNHDCVTARVSEITPNIIILGARSGSRDEFLLAKEKYHLY
CEEEEEECCCCCHHHHCCCCCCCCHHEEEHHHCCCCEEEEECCCCCCCCEEEEECEEEEE
TADDIKDRGIGTVLNEIKALIGDADLYLSIDADSIDCCLTPGLGTPEPFGLTPREVREVV
ECCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCCCCCCCCCCCHHHHHHHH
RALAKNAVGFDYVEVLPNDAGQTAMVAAHIIREFIAGHACKSK
HHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: agmatine; H2O

Specific reaction: agmatine + H2O = putrescine + urea

General reaction: Amidine hydrolysis [C]

Inhibitor: EDTA; EGTA [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2377617 [H]