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The map label for this gene is fwdF [H]

Identifier: 124485225

GI number: 124485225

Start: 374395

End: 375606

Strand: Reverse

Name: fwdF [H]

Synonym: Mlab_0398

Alternate gene names: 124485225

Gene position: 375606-374395 (Counterclockwise)

Preceding gene: 124485226

Following gene: 124485224

Centisome position: 20.81

GC content: 46.45

Gene sequence:

>1212_bases
ATGACGATAAATACTTTGTACCCGAAATATTCAAAAAAAAGAGATAACGATGCGAACATGGTCATGGAGCAGCGTCTTCT
CAAATCGGTATCGCAGCTCATTCTTGACACATCGAGATGCGTCGGGTGCGGAATCTGTCTGGATTCCTGCCCAAAAGATG
CAATCGTTTTACAAAATGCAGGTGTTCTCAAAGGAGAAGGGGCAATCAGTGTTGACCCGGTAAAATGTTCGTACTGCGGT
ATCTGCGCAATTCTCTGCCCGCTGAGGGCAGTGAAAGTCACTGTTAACGGGGAAAAGACATTGGCTATCCTTGATAATGA
AGGATTTCCCCAGTATGATTTTACAACCAGTATCGATGAAAAAAAGTGCAAACGATGTACTGTCTGCAGCGAAGTATGTC
CTGAGGGTGCAATAATTCGGGACATTCCGATCTACGAGGGACAGGACCCGGCAGGTGCCCAGCGTCACACGGCTTTAACA
GCAGATATCACCATGGTCATCTGCCTGCATAAATGTACCGTCTGCGGTGTGTGTGCTTCACTCTGCCCGGCCCTTTCTGT
CGAACGCGACCCCTTTACTGCAGAAAAAATGTGCTTTGGTCCAATCGGATGTCCGGCAGAAAAAAGCGGAATAAAATGCA
ACAGTCAGTCGGGCCAGATCAAATGGGAAAAATCCCTTTGCGATGCCTGCGGTGTCTGTATGGCTGCCTGTCCGGAAGAG
GCAATTCTCGAAGTTACCCGTAAGCTGAAAAAAGACCCGATTCTCCCCGGGAAAGTTACTATCAACAAAGAAAACTGCGT
TACATGCAGCTGGTGTGAGAAAACCTGCCCATATGATGCTGTAGAAGTAACGAAGTTTTTTGAGGGAGAACTCGTCATCG
ACGCAGAAAAATGTCCAGAGGGCTGCAGTACCTGTGTAGAGATTTGTCCCTGCCATGCGATATTTATGGCAGCTCCGGCA
GGTACCAAAGGAGGGAAAAAGGCAAAGAAAGGAAAAAAACTGCAATTATCCATAAATCAGGATTTGTGTATTCTCTGCGG
AGCATGTGTAAATGCGTGTCATGGAGAGAATGCACTCACTCTTCATCGGACACATATCAACGTCAAAGGCACCGAGACCG
ATATGTTCAAGGACATCGCTAAAAGACTCTGCGAAAAGAAAATTTCGTCAGGGATTGACAATACAGCTGATATCAGCGAT
GTCAAAGAATAA

Upstream 100 bases:

>100_bases
ACATACGAATAATGATACCAGAAAGGCAAAAAATGACTTTTGACGATCTGCATTTCACTGTACAGATTTCGAGACTCCCA
AAAAGCCTTGGTAACTGAAA

Downstream 100 bases:

>100_bases
TGATCTAAAATACTGATTGTAGTTCATAGAGAAACACCTCTTTTTTATTTAACGAAGAAACAGCGCGGGGCTGCCGTATC
CAAATCCATATTTAAGTTTC

Product: hypothetical protein

Products: CO2; methanofuran; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MTINTLYPKYSKKRDNDANMVMEQRLLKSVSQLILDTSRCVGCGICLDSCPKDAIVLQNAGVLKGEGAISVDPVKCSYCG
ICAILCPLRAVKVTVNGEKTLAILDNEGFPQYDFTTSIDEKKCKRCTVCSEVCPEGAIIRDIPIYEGQDPAGAQRHTALT
ADITMVICLHKCTVCGVCASLCPALSVERDPFTAEKMCFGPIGCPAEKSGIKCNSQSGQIKWEKSLCDACGVCMAACPEE
AILEVTRKLKKDPILPGKVTINKENCVTCSWCEKTCPYDAVEVTKFFEGELVIDAEKCPEGCSTCVEICPCHAIFMAAPA
GTKGGKKAKKGKKLQLSINQDLCILCGACVNACHGENALTLHRTHINVKGTETDMFKDIAKRLCEKKISSGIDNTADISD
VKE

Sequences:

>Translated_403_residues
MTINTLYPKYSKKRDNDANMVMEQRLLKSVSQLILDTSRCVGCGICLDSCPKDAIVLQNAGVLKGEGAISVDPVKCSYCG
ICAILCPLRAVKVTVNGEKTLAILDNEGFPQYDFTTSIDEKKCKRCTVCSEVCPEGAIIRDIPIYEGQDPAGAQRHTALT
ADITMVICLHKCTVCGVCASLCPALSVERDPFTAEKMCFGPIGCPAEKSGIKCNSQSGQIKWEKSLCDACGVCMAACPEE
AILEVTRKLKKDPILPGKVTINKENCVTCSWCEKTCPYDAVEVTKFFEGELVIDAEKCPEGCSTCVEICPCHAIFMAAPA
GTKGGKKAKKGKKLQLSINQDLCILCGACVNACHGENALTLHRTHINVKGTETDMFKDIAKRLCEKKISSGIDNTADISD
VKE
>Mature_402_residues
TINTLYPKYSKKRDNDANMVMEQRLLKSVSQLILDTSRCVGCGICLDSCPKDAIVLQNAGVLKGEGAISVDPVKCSYCGI
CAILCPLRAVKVTVNGEKTLAILDNEGFPQYDFTTSIDEKKCKRCTVCSEVCPEGAIIRDIPIYEGQDPAGAQRHTALTA
DITMVICLHKCTVCGVCASLCPALSVERDPFTAEKMCFGPIGCPAEKSGIKCNSQSGQIKWEKSLCDACGVCMAACPEEA
ILEVTRKLKKDPILPGKVTINKENCVTCSWCEKTCPYDAVEVTKFFEGELVIDAEKCPEGCSTCVEICPCHAIFMAAPAG
TKGGKKAKKGKKLQLSINQDLCILCGACVNACHGENALTLHRTHINVKGTETDMFKDIAKRLCEKKISSGIDNTADISDV
KE

Specific function: Probable Electron Transfer Protein For Hydrogenase 3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 8 4Fe-4S ferredoxin-type domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR016459 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.2.99.5

Molecular weight: Translated: 43494; Mature: 43363

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
11.4 %Cys+Met (Translated Protein)
9.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
11.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTINTLYPKYSKKRDNDANMVMEQRLLKSVSQLILDTSRCVGCGICLDSCPKDAIVLQNA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHCCCCCEEEEECC
GVLKGEGAISVDPVKCSYCGICAILCPLRAVKVTVNGEKTLAILDNEGFPQYDFTTSIDE
CEEECCCCEEECCCCCHHHHHHHHHHCCEEEEEEECCCEEEEEEECCCCCCCCCCCCCCH
KKCKRCTVCSEVCPEGAIIRDIPIYEGQDPAGAQRHTALTADITMVICLHKCTVCGVCAS
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHH
LCPALSVERDPFTAEKMCFGPIGCPAEKSGIKCNSQSGQIKWEKSLCDACGVCMAACPEE
HHHHCCCCCCCCCHHHHEECCCCCCCCCCCCEECCCCCCEEEHHHHHHHHHHHHHHCCHH
AILEVTRKLKKDPILPGKVTINKENCVTCSWCEKTCPYDAVEVTKFFEGELVIDAEKCPE
HHHHHHHHHHHCCCCCCEEEECCCCCEEEECHHHCCCCCHHHHHHHHCCCEEEEHHHCCH
GCSTCVEICPCHAIFMAAPAGTKGGKKAKKGKKLQLSINQDLCILCGACVNACHGENALT
HHHHHHHHHCHHEEEEECCCCCCCCCHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEE
LHRTHINVKGTETDMFKDIAKRLCEKKISSGIDNTADISDVKE
EEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCC
>Mature Secondary Structure 
TINTLYPKYSKKRDNDANMVMEQRLLKSVSQLILDTSRCVGCGICLDSCPKDAIVLQNA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHCCCCCEEEEECC
GVLKGEGAISVDPVKCSYCGICAILCPLRAVKVTVNGEKTLAILDNEGFPQYDFTTSIDE
CEEECCCCEEECCCCCHHHHHHHHHHCCEEEEEEECCCEEEEEEECCCCCCCCCCCCCCH
KKCKRCTVCSEVCPEGAIIRDIPIYEGQDPAGAQRHTALTADITMVICLHKCTVCGVCAS
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHH
LCPALSVERDPFTAEKMCFGPIGCPAEKSGIKCNSQSGQIKWEKSLCDACGVCMAACPEE
HHHHCCCCCCCCCHHHHEECCCCCCCCCCCCEECCCCCCEEEHHHHHHHHHHHHHHCCHH
AILEVTRKLKKDPILPGKVTINKENCVTCSWCEKTCPYDAVEVTKFFEGELVIDAEKCPE
HHHHHHHHHHHCCCCCCEEEECCCCCEEEECHHHCCCCCHHHHHHHHCCCEEEEHHHCCH
GCSTCVEICPCHAIFMAAPAGTKGGKKAKKGKKLQLSINQDLCILCGACVNACHGENALT
HHHHHHHHHCHHEEEEECCCCCCCCCHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEE
LHRTHINVKGTETDMFKDIAKRLCEKKISSGIDNTADISDVKE
EEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formylmethanofuran; H2O; acceptor

Specific reaction: formylmethanofuran + H2O + acceptor = CO2 + methanofuran + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]