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The map label for this gene is ribE [H]

Identifier: 124485224

GI number: 124485224

Start: 373512

End: 374162

Strand: Reverse

Name: ribE [H]

Synonym: Mlab_0397

Alternate gene names: 124485224

Gene position: 374162-373512 (Counterclockwise)

Preceding gene: 124485225

Following gene: 124485223

Centisome position: 20.73

GC content: 49.16

Gene sequence:

>651_bases
ATGTTCACCGGAATCGTAGAGGAAATTGGAATTGTGACCAAAATCATGAAAGGTCAAAACTCCTCTGTCCTTTCCATTCA
GGGAAACAGGATTTTTGAAGATCTGACGTTAGGAGACAGTGTTGCTGTAAACGGTGTGTGTCTTACCGTAAGCGGTCTGA
ACCGGCAAAGTTTCGATGCCGACACAACAAGCGAAACACTCGCACGAACCTCGCTTGGAGCTCTCAGAACCGGAAGTTCC
GTGAATCTCGAACGTGCCATGTCCGCAAACGGACGCTTCGGAGGACACATCGTCACGGGGCATGTTGACGGGACAGGCAT
TATTTCCACGATCCGCCGGGATGAACGGACCGTCTGGATCACGTTTACTGCAGGTCCGGAGATACTCAAATACATCGTTG
AGAAAGGATCCATCGCGGTAGACGGGATCAGTCTGACCGTGGCTGCCGTAACGCAGGATTCATTCAGCGTTGCAATCATC
CCCCACACAGAAAAATCCACCACTCTCCTCATGAAATCGCAAGGAGAGAGAGTAAATCTGGAGTGTGATATGATTGGAAA
ATACGTCGAACGGTTCATTGCCAAAAGACAGAAGAATGAACCGCAGAAGTGCGTCATAACCGAGGAATACCTCAGCAAAG
CAGGATTTTAG

Upstream 100 bases:

>100_bases
TAACTCATTTTTGATTGGCAACTCAAATAGAGGAATGATTATATGTGTACGAGTCGAATAAATACTAAGGCTGGTTCAAC
CTGCACAGGAGTAAATAATC

Downstream 100 bases:

>100_bases
CTATGTTTACATTTAACACGATCGAAGAGGCCCTGAAATCCCTTCAAAACGGAGAGATGATCATCGTCACCGATGATGAG
AACCGAGAGAACGAAGGTGA

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MFTGIVEEIGIVTKIMKGQNSSVLSIQGNRIFEDLTLGDSVAVNGVCLTVSGLNRQSFDADTTSETLARTSLGALRTGSS
VNLERAMSANGRFGGHIVTGHVDGTGIISTIRRDERTVWITFTAGPEILKYIVEKGSIAVDGISLTVAAVTQDSFSVAII
PHTEKSTTLLMKSQGERVNLECDMIGKYVERFIAKRQKNEPQKCVITEEYLSKAGF

Sequences:

>Translated_216_residues
MFTGIVEEIGIVTKIMKGQNSSVLSIQGNRIFEDLTLGDSVAVNGVCLTVSGLNRQSFDADTTSETLARTSLGALRTGSS
VNLERAMSANGRFGGHIVTGHVDGTGIISTIRRDERTVWITFTAGPEILKYIVEKGSIAVDGISLTVAAVTQDSFSVAII
PHTEKSTTLLMKSQGERVNLECDMIGKYVERFIAKRQKNEPQKCVITEEYLSKAGF
>Mature_216_residues
MFTGIVEEIGIVTKIMKGQNSSVLSIQGNRIFEDLTLGDSVAVNGVCLTVSGLNRQSFDADTTSETLARTSLGALRTGSS
VNLERAMSANGRFGGHIVTGHVDGTGIISTIRRDERTVWITFTAGPEILKYIVEKGSIAVDGISLTVAAVTQDSFSVAII
PHTEKSTTLLMKSQGERVNLECDMIGKYVERFIAKRQKNEPQKCVITEEYLSKAGF

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=210, Percent_Identity=38.5714285714286, Blast_Score=124, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6319733, Length=215, Percent_Identity=39.5348837209302, Blast_Score=139, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 23350; Mature: 23350

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIVEEIGIVTKIMKGQNSSVLSIQGNRIFEDLTLGDSVAVNGVCLTVSGLNRQSFDA
CCCCHHHHHHHHHHHHCCCCCCEEEEECCEEEEEECCCCCEEECEEEEEEECCCCCCCCC
DTTSETLARTSLGALRTGSSVNLERAMSANGRFGGHIVTGHVDGTGIISTIRRDERTVWI
CCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEEEEEECCCHHHHHHCCCCEEEEE
TFTAGPEILKYIVEKGSIAVDGISLTVAAVTQDSFSVAIIPHTEKSTTLLMKSQGERVNL
EEECCHHHHHHHHHCCCEEEECEEEEEEEEECCCEEEEEEECCCCCEEEEEECCCCEEEE
ECDMIGKYVERFIAKRQKNEPQKCVITEEYLSKAGF
EHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHCCC
>Mature Secondary Structure
MFTGIVEEIGIVTKIMKGQNSSVLSIQGNRIFEDLTLGDSVAVNGVCLTVSGLNRQSFDA
CCCCHHHHHHHHHHHHCCCCCCEEEEECCEEEEEECCCCCEEECEEEEEEECCCCCCCCC
DTTSETLARTSLGALRTGSSVNLERAMSANGRFGGHIVTGHVDGTGIISTIRRDERTVWI
CCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEEEEEECCCHHHHHHCCCCEEEEE
TFTAGPEILKYIVEKGSIAVDGISLTVAAVTQDSFSVAIIPHTEKSTTLLMKSQGERVNL
EEECCHHHHHHHHHCCCEEEECEEEEEEEEECCCEEEEEEECCCCCEEEEEECCCCEEEE
ECDMIGKYVERFIAKRQKNEPQKCVITEEYLSKAGF
EHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8522537 [H]