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The map label for this gene is nfo

Identifier: 124485053

GI number: 124485053

Start: 212135

End: 212980

Strand: Reverse

Name: nfo

Synonym: Mlab_0226

Alternate gene names: 124485053

Gene position: 212980-212135 (Counterclockwise)

Preceding gene: 124485054

Following gene: 124485051

Centisome position: 11.8

GC content: 52.25

Gene sequence:

>846_bases
ATGATTAAACTCGGATTCCACGTATCGATCGCCGGCTCCTTACCTCTTGCCGTGTCCAGGGCCCAGGAAGCGGGATGCGA
CACCTTCCAGATATTTACGCGGAGTCCGCGTGTCTGGGCTGCCAAACCGATTGAACCCTCGATTGCAGAAGCGTTTATCG
ATGCATTAAACATTTCGGGAATCGGGCCGGTCGTTGATCACATGCCCTACCTTCCAAATCCGGCAGCAGAAAAACCCGAG
ATATATGCAAGATCGATTTTCACCATGACCGAAGAGCTTGACCGCTGCGATCAGCTCAAAATCCCCTATCTCGTGACCCA
TCTGGGTCATCACGGAAAAGAAGACGGACATAAAAAAGGTCAGGAGAAAGTCATCGCGGCGATCGGTCAGGCGCTGGATG
AGTCGGAAGGCGAGACAATGATCCTGCTCGAAAACACCGCGAACGAAAAAAATACCGTTGGGGGAACATTCACGGACATA
GGCGTGATCTCCGACGCTCTTTCAAACGAACGAAGGGTCGGATTTTGTTTTGACACCTGTCATGCAGCAGCAGCCGGCTA
CGATTTGAAAGGTCACGGGGCGGAGACCGTGTTCGGCTGGTTCAATGATGAGGCAGGAAGCCTTGATCGACTCAAAGTCA
TTCATCTCAACGACATGAAAGGCGGGGTCGGTTCCCATCTGGACCGACACGAACATCTGGGGCTTGGATATCTTGGAGAA
GAGACCATTCATGATGTCCTGACGTTTCCAAAGATCTCGCACTGCGCCTTCATTATGGAGACCCCCTCTGACGAAATCAG
GACCGATAAAGATAACCTGGCCGTCGCACGAAGACTGGCCGTGTAA

Upstream 100 bases:

>100_bases
ATGTACAACGGATACTTCGGCGCAGTGGTTCACTGGGGAGCAAACCCGAAAACCATTGCCGATGCACTTTTTGCAATGAT
AAAAGAATGGAGAGAACGAA

Downstream 100 bases:

>100_bases
TTTCAGTATGAGCGAAAGCTCAAAAATCAGGGAAGCGGATGAAAGCCCAGTGCCGAAACGAGTTCGTCGACGTTCAAAAC
GACCTCTGCCTCCATCTCGA

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MIKLGFHVSIAGSLPLAVSRAQEAGCDTFQIFTRSPRVWAAKPIEPSIAEAFIDALNISGIGPVVDHMPYLPNPAAEKPE
IYARSIFTMTEELDRCDQLKIPYLVTHLGHHGKEDGHKKGQEKVIAAIGQALDESEGETMILLENTANEKNTVGGTFTDI
GVISDALSNERRVGFCFDTCHAAAAGYDLKGHGAETVFGWFNDEAGSLDRLKVIHLNDMKGGVGSHLDRHEHLGLGYLGE
ETIHDVLTFPKISHCAFIMETPSDEIRTDKDNLAVARRLAV

Sequences:

>Translated_281_residues
MIKLGFHVSIAGSLPLAVSRAQEAGCDTFQIFTRSPRVWAAKPIEPSIAEAFIDALNISGIGPVVDHMPYLPNPAAEKPE
IYARSIFTMTEELDRCDQLKIPYLVTHLGHHGKEDGHKKGQEKVIAAIGQALDESEGETMILLENTANEKNTVGGTFTDI
GVISDALSNERRVGFCFDTCHAAAAGYDLKGHGAETVFGWFNDEAGSLDRLKVIHLNDMKGGVGSHLDRHEHLGLGYLGE
ETIHDVLTFPKISHCAFIMETPSDEIRTDKDNLAVARRLAV
>Mature_281_residues
MIKLGFHVSIAGSLPLAVSRAQEAGCDTFQIFTRSPRVWAAKPIEPSIAEAFIDALNISGIGPVVDHMPYLPNPAAEKPE
IYARSIFTMTEELDRCDQLKIPYLVTHLGHHGKEDGHKKGQEKVIAAIGQALDESEGETMILLENTANEKNTVGGTFTDI
GVISDALSNERRVGFCFDTCHAAAAGYDLKGHGAETVFGWFNDEAGSLDRLKVIHLNDMKGGVGSHLDRHEHLGLGYLGE
ETIHDVLTFPKISHCAFIMETPSDEIRTDKDNLAVARRLAV

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=269, Percent_Identity=32.7137546468402, Blast_Score=134, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17531193, Length=266, Percent_Identity=34.9624060150376, Blast_Score=157, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6322735, Length=268, Percent_Identity=32.089552238806, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_METLZ (A2SPZ6)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001029669.1
- ProteinModelPortal:   A2SPZ6
- SMR:   A2SPZ6
- STRING:   A2SPZ6
- GeneID:   4795654
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_0226
- eggNOG:   arNOG07405
- HOGENOM:   HBG565018
- OMA:   QIALETM
- PhylomeDB:   A2SPZ6
- BioCyc:   MLAB410358:MLAB_0226-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 30642; Mature: 30642

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLGFHVSIAGSLPLAVSRAQEAGCDTFQIFTRSPRVWAAKPIEPSIAEAFIDALNISG
CEEEEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCEEECCCCCHHHHHHHHHHHCCCC
IGPVVDHMPYLPNPAAEKPEIYARSIFTMTEELDRCDQLKIPYLVTHLGHHGKEDGHKKG
CCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHH
QEKVIAAIGQALDESEGETMILLENTANEKNTVGGTFTDIGVISDALSNERRVGFCFDTC
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEHHHH
HAAAAGYDLKGHGAETVFGWFNDEAGSLDRLKVIHLNDMKGGVGSHLDRHEHLGLGYLGE
HHHHCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCHHHHCHHHHCCCCCCCCH
ETIHDVLTFPKISHCAFIMETPSDEIRTDKDNLAVARRLAV
HHHHHHHCCCCCCCEEEEEECCCHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MIKLGFHVSIAGSLPLAVSRAQEAGCDTFQIFTRSPRVWAAKPIEPSIAEAFIDALNISG
CEEEEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCEEECCCCCHHHHHHHHHHHCCCC
IGPVVDHMPYLPNPAAEKPEIYARSIFTMTEELDRCDQLKIPYLVTHLGHHGKEDGHKKG
CCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHH
QEKVIAAIGQALDESEGETMILLENTANEKNTVGGTFTDIGVISDALSNERRVGFCFDTC
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEHHHH
HAAAAGYDLKGHGAETVFGWFNDEAGSLDRLKVIHLNDMKGGVGSHLDRHEHLGLGYLGE
HHHHCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCHHHHCHHHHCCCCCCCCH
ETIHDVLTFPKISHCAFIMETPSDEIRTDKDNLAVARRLAV
HHHHHHHCCCCCCCEEEEEECCCHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA