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The map label for this gene is nudF [H]

Identifier: 124485012

GI number: 124485012

Start: 171509

End: 172516

Strand: Reverse

Name: nudF [H]

Synonym: Mlab_0185

Alternate gene names: 124485012

Gene position: 172516-171509 (Counterclockwise)

Preceding gene: 124485013

Following gene: 124485011

Centisome position: 9.56

GC content: 51.88

Gene sequence:

>1008_bases
TTGAGTACAGTAGTCATTTATAAATCAAAATACGGCTCCACGGAACAGTATGCAAAATGGATCGCAGAAGAGCTGGGTGC
AGACCTGAAAAATGCGGATCACCTCAAACTGGACGATCTTCTCCCCTACGACACGATCGTGTACGGAGCTGGCGTTTACG
TTGGATGTATCGCCGGCATCGCGCTGATCGCGGACAACTACGAACAGCTCAAGGATAAGAAACTCATCGTCTTCACCGTC
GGGCTGACCGATCCTGCTGATGAGAAAAAGTACGATGATCTCCTTACCAAAAACTTCACCTGGACGATGAAGGACGCCGT
GCCTGCGTTCCATTTCCGCGGAGCACTCGATTACAAAAAGATCGGGCTCGGCAACAAACTGATGATGAAGAAAATGAAGA
TCCCGGAGGCGAATTACGTGAGCAAAGAACAGATCCAGCCGCTGCTTAAATCTCTCGGGTCATCAAAACCGGCAAACAAA
CCGGAAAACACCACGACAAAGATCTACGACACGCCAAGACTCACCATCGAAACCCGGCACGTCCATCTCCCAAACGGTAA
AGACAGAAACTACCTCTTTGTCCAGCCGGTTCCGGCGGTCTGTATCCTCCCAACCGACGAGACCCATGTATACCTGATCA
AACAGTACCGTGCGGTGATCAACGAGTACATCCTCGAGGTCCCGGCAGGAGGTATGGACAACGGAAGCGAGACCCCGCTC
GAATGCGCAAAAAGGGAACTCGCAGAGGAAGCACGTCTCTCGGCAAAGGAGTTCGTCCCGAAAGGATACGTCTACTCGAC
GCCCGGATTCTGCACGGAAAAACTGTGGCTCTTCGAAGCACGGGGACTTGCTCCATGCGAAGACTGCGCAAGAGACGAGG
ATGAGATCATCGATGTCGTGAAAGTGCCCAAAGCCGAAGTTTTTGCCATGATCGACCGTGGAGAGATCGTCGATGCAAAA
ACGATCGCACTCCTGACAAGGTCGCTGGCGAAGCTTAATAGTGACTGA

Upstream 100 bases:

>100_bases
GAAAGCAGAATAATACCCTCCTCTTTTTTCAATATCGGCATTCTCCGTACTAAAACACTTATTCCATCACGACCAATTCA
AAATAACAGAAGGGATAAAC

Downstream 100 bases:

>100_bases
ATAGAAATATTATCCGACATCTATGGATATGAAAAATGCAGTTAAATACCTGCTTTTCGATCAAAAGCAGTTCTTCGAAA
AGGCTCCCAAAGGTCTTCTG

Product: hypothetical protein

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MSTVVIYKSKYGSTEQYAKWIAEELGADLKNADHLKLDDLLPYDTIVYGAGVYVGCIAGIALIADNYEQLKDKKLIVFTV
GLTDPADEKKYDDLLTKNFTWTMKDAVPAFHFRGALDYKKIGLGNKLMMKKMKIPEANYVSKEQIQPLLKSLGSSKPANK
PENTTTKIYDTPRLTIETRHVHLPNGKDRNYLFVQPVPAVCILPTDETHVYLIKQYRAVINEYILEVPAGGMDNGSETPL
ECAKRELAEEARLSAKEFVPKGYVYSTPGFCTEKLWLFEARGLAPCEDCARDEDEIIDVVKVPKAEVFAMIDRGEIVDAK
TIALLTRSLAKLNSD

Sequences:

>Translated_335_residues
MSTVVIYKSKYGSTEQYAKWIAEELGADLKNADHLKLDDLLPYDTIVYGAGVYVGCIAGIALIADNYEQLKDKKLIVFTV
GLTDPADEKKYDDLLTKNFTWTMKDAVPAFHFRGALDYKKIGLGNKLMMKKMKIPEANYVSKEQIQPLLKSLGSSKPANK
PENTTTKIYDTPRLTIETRHVHLPNGKDRNYLFVQPVPAVCILPTDETHVYLIKQYRAVINEYILEVPAGGMDNGSETPL
ECAKRELAEEARLSAKEFVPKGYVYSTPGFCTEKLWLFEARGLAPCEDCARDEDEIIDVVKVPKAEVFAMIDRGEIVDAK
TIALLTRSLAKLNSD
>Mature_334_residues
STVVIYKSKYGSTEQYAKWIAEELGADLKNADHLKLDDLLPYDTIVYGAGVYVGCIAGIALIADNYEQLKDKKLIVFTVG
LTDPADEKKYDDLLTKNFTWTMKDAVPAFHFRGALDYKKIGLGNKLMMKKMKIPEANYVSKEQIQPLLKSLGSSKPANKP
ENTTTKIYDTPRLTIETRHVHLPNGKDRNYLFVQPVPAVCILPTDETHVYLIKQYRAVINEYILEVPAGGMDNGSETPLE
CAKRELAEEARLSAKEFVPKGYVYSTPGFCTEKLWLFEARGLAPCEDCARDEDEIIDVVKVPKAEVFAMIDRGEIVDAKT
IALLTRSLAKLNSD

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 37540; Mature: 37408

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00893 NUDIX ; PS00201 FLAVODOXIN ; PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVVIYKSKYGSTEQYAKWIAEELGADLKNADHLKLDDLLPYDTIVYGAGVYVGCIAGI
CCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHCCHHHHHHHHHH
ALIADNYEQLKDKKLIVFTVGLTDPADEKKYDDLLTKNFTWTMKDAVPAFHFRGALDYKK
HHHHCCHHHHCCCEEEEEEEECCCCCCHHHHHHHHHCCCEEEHHHCCCHHHHCCCCCHHH
IGLGNKLMMKKMKIPEANYVSKEQIQPLLKSLGSSKPANKPENTTTKIYDTPRLTIETRH
CCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEEEEEE
VHLPNGKDRNYLFVQPVPAVCILPTDETHVYLIKQYRAVINEYILEVPAGGMDNGSETPL
EECCCCCCCCEEEECCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
ECAKRELAEEARLSAKEFVPKGYVYSTPGFCTEKLWLFEARGLAPCEDCARDEDEIIDVV
HHHHHHHHHHHHCCHHHCCCCCEEECCCCCCHHHEEEEECCCCCCHHHHCCCHHHHHHHH
KVPKAEVFAMIDRGEIVDAKTIALLTRSLAKLNSD
CCCCHHEEEEECCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STVVIYKSKYGSTEQYAKWIAEELGADLKNADHLKLDDLLPYDTIVYGAGVYVGCIAGI
CEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHCCHHHHHHHHHH
ALIADNYEQLKDKKLIVFTVGLTDPADEKKYDDLLTKNFTWTMKDAVPAFHFRGALDYKK
HHHHCCHHHHCCCEEEEEEEECCCCCCHHHHHHHHHCCCEEEHHHCCCHHHHCCCCCHHH
IGLGNKLMMKKMKIPEANYVSKEQIQPLLKSLGSSKPANKPENTTTKIYDTPRLTIETRH
CCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEEEEEE
VHLPNGKDRNYLFVQPVPAVCILPTDETHVYLIKQYRAVINEYILEVPAGGMDNGSETPL
EECCCCCCCCEEEECCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
ECAKRELAEEARLSAKEFVPKGYVYSTPGFCTEKLWLFEARGLAPCEDCARDEDEIIDVV
HHHHHHHHHHHHCCHHHCCCCCEEECCCCCCHHHEEEEECCCCCCHHHHCCCHHHHHHHH
KVPKAEVFAMIDRGEIVDAKTIALLTRSLAKLNSD
CCCCHHEEEEECCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10542272 [H]