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The map label for this gene is nifK [H]

Identifier: 124484990

GI number: 124484990

Start: 148281

End: 149414

Strand: Reverse

Name: nifK [H]

Synonym: Mlab_0163

Alternate gene names: 124484990

Gene position: 149414-148281 (Counterclockwise)

Preceding gene: 124484991

Following gene: 124484987

Centisome position: 8.28

GC content: 52.47

Gene sequence:

>1134_bases
ATGGATGAAGGACTGTGCGTAAATCCGGTCTGGCCGTGCGCGATGACCGGGGCCTGTTCAGTGCTTGCCGGCTTCTCCGG
ACTGAACGTGCTGATCCACGGTTCATCCGGCTGTTATTATTATCCCCGGTCGCTGCTGAAGGTTCCTTTGTTCAGCACGT
ATCTTCTGGAGTCAGAGATCGTGTTCGGTACGGTCGAGCGCCTGAAAGAGGTCGTGAACACTCTTTCAACGTCGAAGCGA
CCGATCGCCGTTTTAAACACCTGTATCCCGGCACTCACCGGCGAAGATCTTTCGGGGGCATTTTCCGAAGAGGAGACAAT
TTTTGTCGATGCGCCCGGATTCATCGGGAGTGTCGAAGATGGAGCAAAGATCGCCTTTGAACGGCTGGGAATCGAAACGG
ATGCATCAAGGGAAGGCGTGAACATCGACGGGGTTTCCCTTCTGGATCTATTCTGGCGGGGAAATCTGCACGAAGCAAAA
CGGATTCTCACAGAGATGGGGATCCCGACCGCTCTGTGTCTTGCCAAAGACAGCTATGAAAATCTGCGAAAAGGAGCTGC
TTTGCATACCGTCTCGGTAAACCCGTCGTATCCATCCGGCGTTGGAACGATGCTCGGCTCGTTTTTGTTCCCCGACCTGA
AAGATACCTGTGCAAAACTCGCAGATATATTTCCCAACGCCGATATCGACCCGCTCCTCGAAGAGTGGAATCTTGCCGAC
GAGCAGCTGTTCTACTCAAGCGACAAATATCTGCGAAAATATGAACCACCGGTCGTTGCCGTATGTGCTCAGGAAAGTTA
CGCACTGTTTGCAAAATCGATGATGGAGCGCTACTTCGGGGCGGACGTTCCGGTGATGCTTGCACGAAACCATGATGCGG
TAAGCATTCCCTCTGAAACCGATCTGACGAAGATTGCAGGGCATATTGCCGGCTGCCGGCCGGATCTTATCCTTGGATCA
ACGTTCGAAGCAAATGGTTATCCAAACGCTGCATTCCTCGGGATAACGCCGCCTGACAGAAGCCGGGTCTCCATAGCGGC
ACGACCGATTGCAGGAATAGAGGGCGGAATCATGTTTTTAGAGAACGTGCTCAATACCTTGATTGATGCAACTTCGTCAA
AGCAGAAAAAGTGA

Upstream 100 bases:

>100_bases
GAATCGCCGACCGGAATCAAGGTCAGGCTTCCGTTCTATCTGCCGGTCGGTGTGTCCGGGACCGTGAAGATGCTCCATCT
TTGGAGGAGGACGATCCTGC

Downstream 100 bases:

>100_bases
TGGATTTTCGTATTTTATTATAAATTGGATCAGTCGACTTTGATCGTCGTCAGTTTGACCGACTCGACACCTTTCAAAGA
CATCAGCCGTTCAGCGAGCT

Product: hypothetical protein

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MDEGLCVNPVWPCAMTGACSVLAGFSGLNVLIHGSSGCYYYPRSLLKVPLFSTYLLESEIVFGTVERLKEVVNTLSTSKR
PIAVLNTCIPALTGEDLSGAFSEEETIFVDAPGFIGSVEDGAKIAFERLGIETDASREGVNIDGVSLLDLFWRGNLHEAK
RILTEMGIPTALCLAKDSYENLRKGAALHTVSVNPSYPSGVGTMLGSFLFPDLKDTCAKLADIFPNADIDPLLEEWNLAD
EQLFYSSDKYLRKYEPPVVAVCAQESYALFAKSMMERYFGADVPVMLARNHDAVSIPSETDLTKIAGHIAGCRPDLILGS
TFEANGYPNAAFLGITPPDRSRVSIAARPIAGIEGGIMFLENVLNTLIDATSSKQKK

Sequences:

>Translated_377_residues
MDEGLCVNPVWPCAMTGACSVLAGFSGLNVLIHGSSGCYYYPRSLLKVPLFSTYLLESEIVFGTVERLKEVVNTLSTSKR
PIAVLNTCIPALTGEDLSGAFSEEETIFVDAPGFIGSVEDGAKIAFERLGIETDASREGVNIDGVSLLDLFWRGNLHEAK
RILTEMGIPTALCLAKDSYENLRKGAALHTVSVNPSYPSGVGTMLGSFLFPDLKDTCAKLADIFPNADIDPLLEEWNLAD
EQLFYSSDKYLRKYEPPVVAVCAQESYALFAKSMMERYFGADVPVMLARNHDAVSIPSETDLTKIAGHIAGCRPDLILGS
TFEANGYPNAAFLGITPPDRSRVSIAARPIAGIEGGIMFLENVLNTLIDATSSKQKK
>Mature_377_residues
MDEGLCVNPVWPCAMTGACSVLAGFSGLNVLIHGSSGCYYYPRSLLKVPLFSTYLLESEIVFGTVERLKEVVNTLSTSKR
PIAVLNTCIPALTGEDLSGAFSEEETIFVDAPGFIGSVEDGAKIAFERLGIETDASREGVNIDGVSLLDLFWRGNLHEAK
RILTEMGIPTALCLAKDSYENLRKGAALHTVSVNPSYPSGVGTMLGSFLFPDLKDTCAKLADIFPNADIDPLLEEWNLAD
EQLFYSSDKYLRKYEPPVVAVCAQESYALFAKSMMERYFGADVPVMLARNHDAVSIPSETDLTKIAGHIAGCRPDLILGS
TFEANGYPNAAFLGITPPDRSRVSIAARPIAGIEGGIMFLENVLNTLIDATSSKQKK

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 40768; Mature: 40768

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEGLCVNPVWPCAMTGACSVLAGFSGLNVLIHGSSGCYYYPRSLLKVPLFSTYLLESEI
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEECCHHHHHHHHHHHHHHHHHH
VFGTVERLKEVVNTLSTSKRPIAVLNTCIPALTGEDLSGAFSEEETIFVDAPGFIGSVED
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCC
GAKIAFERLGIETDASREGVNIDGVSLLDLFWRGNLHEAKRILTEMGIPTALCLAKDSYE
CHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCEEHHCCHHHH
NLRKGAALHTVSVNPSYPSGVGTMLGSFLFPDLKDTCAKLADIFPNADIDPLLEEWNLAD
HHHCCCEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCH
EQLFYSSDKYLRKYEPPVVAVCAQESYALFAKSMMERYFGADVPVMLARNHDAVSIPSET
HHHHHCCCHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCEEEECCCCCEECCCCC
DLTKIAGHIAGCRPDLILGSTFEANGYPNAAFLGITPPDRSRVSIAARPIAGIEGGIMFL
HHHHHHHHHCCCCCCEEECCEEECCCCCCEEEEECCCCCCCCEEEEECCCCCCCHHHHHH
ENVLNTLIDATSSKQKK
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDEGLCVNPVWPCAMTGACSVLAGFSGLNVLIHGSSGCYYYPRSLLKVPLFSTYLLESEI
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEECCHHHHHHHHHHHHHHHHHH
VFGTVERLKEVVNTLSTSKRPIAVLNTCIPALTGEDLSGAFSEEETIFVDAPGFIGSVED
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCC
GAKIAFERLGIETDASREGVNIDGVSLLDLFWRGNLHEAKRILTEMGIPTALCLAKDSYE
CHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCEEHHCCHHHH
NLRKGAALHTVSVNPSYPSGVGTMLGSFLFPDLKDTCAKLADIFPNADIDPLLEEWNLAD
HHHCCCEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCH
EQLFYSSDKYLRKYEPPVVAVCAQESYALFAKSMMERYFGADVPVMLARNHDAVSIPSET
HHHHHCCCHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCEEEECCCCCEECCCCC
DLTKIAGHIAGCRPDLILGSTFEANGYPNAAFLGITPPDRSRVSIAARPIAGIEGGIMFL
HHHHHHHHHCCCCCCEEECCEEECCCCCCEEEEECCCCCCCCEEEEECCCCCCCHHHHHH
ENVLNTLIDATSSKQKK
HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8574444 [H]