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The map label for this gene is pyrF [H]

Identifier: 124484981

GI number: 124484981

Start: 139217

End: 139855

Strand: Reverse

Name: pyrF [H]

Synonym: Mlab_0153

Alternate gene names: 124484981

Gene position: 139855-139217 (Counterclockwise)

Preceding gene: 124484982

Following gene: 124484976

Centisome position: 7.75

GC content: 54.77

Gene sequence:

>639_bases
ATGGCCGAACTTCTGCTCGCACTCGATGTAAAAGGTGCCGAAGAAGCGATCCGCGTATCCCGGGCTTGCGAAGGGGAACT
CGATGCGATAAAAATCGGTTATCCCCTGATTCTTTCAACCGGGATGGGGATCGTAAAAGAGCTTGCAAAATCCAATATTC
CGATCATTGCAGATTTCAAAGTTGCCGACATCCCGAACACCAACGCCCTTATCTGCGAAGAGGTCTTTGCAGCAGGATGT
TCGGGCATCATCACCCATGTATTCTGCGGATCGGACTCTCTTCAGGCAATCGTGGACGGAGCGCACGATCACGGGGGACG
TGCCTTTGCGGTCTGCGAGATGAGTCACCCCGGAGCGCTCGATTTTCTGAGCGGGGCAAATGCCGAGCGGATGGCTAAAA
TGGCAAAAGAAGCAGGAGCCGACGGAATCATCGCTCCGGCGACGAGACCGGAGCGCACCGAAATTCTGCGATCCATCATC
GGCAATTCAATGAAGATCTATTCGCCGGGCGTGGGCGCTCAGGGAGCACAGCCCGAAGATATCAAAAAATATGTGGACGG
GATCATTGTCGGCCGTGCCATTTACGAATCGGCAGATCCGGGGAAAGCAGCGCATGAATTCCGCCTGCGTGCCCTCTGA

Upstream 100 bases:

>100_bases
GATCGGCGAAGGAGGGACCGGCGTTACCGTGTACGGCGATGCCACAATAACACTCCCCATCCTTGCAGCAGCAACGCTGG
AACGTATGGAGAGAAAATAA

Downstream 100 bases:

>100_bases
TTTCCTCTGATTTTTTTTCTTATTTTCTCGGGAAGTAGGACAGCAGATCCCATGCAAGGGATTCGGCCTTCTTTTCATTG
GTCATCATCGTATCCAGACG

Product: orotidine-5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MAELLLALDVKGAEEAIRVSRACEGELDAIKIGYPLILSTGMGIVKELAKSNIPIIADFKVADIPNTNALICEEVFAAGC
SGIITHVFCGSDSLQAIVDGAHDHGGRAFAVCEMSHPGALDFLSGANAERMAKMAKEAGADGIIAPATRPERTEILRSII
GNSMKIYSPGVGAQGAQPEDIKKYVDGIIVGRAIYESADPGKAAHEFRLRAL

Sequences:

>Translated_212_residues
MAELLLALDVKGAEEAIRVSRACEGELDAIKIGYPLILSTGMGIVKELAKSNIPIIADFKVADIPNTNALICEEVFAAGC
SGIITHVFCGSDSLQAIVDGAHDHGGRAFAVCEMSHPGALDFLSGANAERMAKMAKEAGADGIIAPATRPERTEILRSII
GNSMKIYSPGVGAQGAQPEDIKKYVDGIIVGRAIYESADPGKAAHEFRLRAL
>Mature_211_residues
AELLLALDVKGAEEAIRVSRACEGELDAIKIGYPLILSTGMGIVKELAKSNIPIIADFKVADIPNTNALICEEVFAAGCS
GIITHVFCGSDSLQAIVDGAHDHGGRAFAVCEMSHPGALDFLSGANAERMAKMAKEAGADGIIAPATRPERTEILRSIIG
NSMKIYSPGVGAQGAQPEDIKKYVDGIIVGRAIYESADPGKAAHEFRLRAL

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=220, Percent_Identity=25, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 22261; Mature: 22130

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELLLALDVKGAEEAIRVSRACEGELDAIKIGYPLILSTGMGIVKELAKSNIPIIADFK
CCCEEEEEECCCHHHHHHHHHHCCCCCCEEEECCHHHHHCCHHHHHHHHHCCCCEEEEEE
VADIPNTNALICEEVFAAGCSGIITHVFCGSDSLQAIVDGAHDHGGRAFAVCEMSHPGAL
EECCCCCCHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCH
DFLSGANAERMAKMAKEAGADGIIAPATRPERTEILRSIIGNSMKIYSPGVGAQGAQPED
HHHCCCCHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCHHH
IKKYVDGIIVGRAIYESADPGKAAHEFRLRAL
HHHHHHHHHHHHHHHCCCCCCCHHHHHEECCC
>Mature Secondary Structure 
AELLLALDVKGAEEAIRVSRACEGELDAIKIGYPLILSTGMGIVKELAKSNIPIIADFK
CCEEEEEECCCHHHHHHHHHHCCCCCCEEEECCHHHHHCCHHHHHHHHHCCCCEEEEEE
VADIPNTNALICEEVFAAGCSGIITHVFCGSDSLQAIVDGAHDHGGRAFAVCEMSHPGAL
EECCCCCCHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCH
DFLSGANAERMAKMAKEAGADGIIAPATRPERTEILRSIIGNSMKIYSPGVGAQGAQPED
HHHCCCCHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCHHH
IKKYVDGIIVGRAIYESADPGKAAHEFRLRAL
HHHHHHHHHHHHHHHCCCCCCCHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]