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The map label for this gene is frdB [C]

Identifier: 124484967

GI number: 124484967

Start: 125535

End: 126980

Strand: Reverse

Name: frdB [C]

Synonym: Mlab_0139

Alternate gene names: 124484967

Gene position: 126980-125535 (Counterclockwise)

Preceding gene: 124484968

Following gene: 124484953

Centisome position: 7.04

GC content: 54.22

Gene sequence:

>1446_bases
ATGAAAGTAACCATCTCCCGTTTCGATCCGGCAACCGGAGAACCACCGAAATTTGTAGAGTATGATGTCAGTGTAAAAGA
TTCGGCACGCGTACTCAATGTTTTGGATGCAGTTCACGATACGATCGACCCAGAGTTCGCCTACCGGCATTGCTGCCGGG
GAGGGCAGTGTGGCTCGTGCGCCGTTCGCGTGAACGGCGAACCTGTTCTTGCCTGCATGCAGGAAGCACGCGAAGGCGAT
ATCATCGAACCCCTCGAACTGCCGAGGATCAGAGACCTGGTCACTGATATTGCCCCGGTCATGGCACAGATGGCCTGGAT
GAACAGCGGATGCGACTGCACGCCGGTCACGCAGCAGGAGATCGAGGAGATGAAACCCTTGCGGGAATGCATCGAATGCT
ACTCATGTATCTCCTCATGTCCCGCGATCGCGGCCTCGGATTACAAAGGCCCGACGATCATGCGGCAGGAGCAGAGACTT
AATCTCGATCCCCGGGACAAAGGGGACCGTATCCTTGAAGCGATCGACAAGGGACTCTTTTCCTGCACGACCTGCCATAA
ATGTGTCGAGGTCTGCCCAAAAGAGATCGAGACGCCACGAAAAGCTATCGAAAAACTCCGGGCCGAGGCAGCAAAGAGGG
GACTCTCCCTTCCGGCACACAAGGCCCTTGCTTCTCTTATCGAGAAGACCGGCCGGTCGATTGAGCGAAAGGAGCCGACC
TTCCTTGAACGGGTGCCGGATGTTATCGAGCCGGAAGGAGAGGTCAGAGCAACGGTAGGATTTTTCGTTGGATGTATGTT
CAACGGACGAGTCATCCAGCCGGCTTTGGATGCGATGGAGGTTCTCAGGAAAAACGGGATAAGAGTCATCATTCCCAAGG
ATCAGGTCTGCTGCGGATCGCCGCTGATCAGAACCGGACTCTGCGGGTTTATTCCTGAACTGCAGAAACGAAACATCGAT
GCATTCGTCAAAGCAGGCGTGGACACCGTCCTGACGATCTGTGCTGGGTGCGGTTCAACGCTGAAAAACGATTACGACAC
CCCGTTCAGAGTTGTGGATATTACGGAATTCCTGTGCGAACTTGGATTTGAAATCCCGGAAAAAGTGGAAGGGACTTATA
CGTATCATGATCCGTGTCATCTCCTTCGGGGACAGGGAATCTCGGAACAACCCAGAATTCTCCTGAACTCGGTTGCGGAA
AAGTTCGTGGAGATGCCGGCACGATGCTGCGGAGCTGGCGGCGGCGTGAAGTCGGGTCTCCCTGAACAGGCGGCGGCAAT
TGGAGCAGTGCGTGCCGAGATGGTGAAAGAAACCGGAGCCGATTACATCGTAACGGTCTGCCCGTTCTGCGAATTTCACC
TTCATCAGGTGACCGGACTGCCGGTAAAGAATATCGCGACCCTGATGCTGGAAGGCTACCGTAAACAGGAAAAGAAAATA
AATTAA

Upstream 100 bases:

>100_bases
GGACCGATGTGAAGCAGAGCTGGACGAACGAGACATCGCCGTTCGGCCACACCTTCTTTGTCAAAGGCAAATCAGGGATA
GAAATTCTGGAGGATAGATT

Downstream 100 bases:

>100_bases
TAAAAAAATTATTCTTTTTTCTGGCCGTTTTTCGCTGCAAGAGCACTCATATAGGCACTCACTGCCGCCGTGACCGCGAT
GGTTTTCTCTTTTCCGGGAC

Product: succinate dehydrogenase/fumarate reductase iron-sulfur subunit

Products: fumarate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MKVTISRFDPATGEPPKFVEYDVSVKDSARVLNVLDAVHDTIDPEFAYRHCCRGGQCGSCAVRVNGEPVLACMQEAREGD
IIEPLELPRIRDLVTDIAPVMAQMAWMNSGCDCTPVTQQEIEEMKPLRECIECYSCISSCPAIAASDYKGPTIMRQEQRL
NLDPRDKGDRILEAIDKGLFSCTTCHKCVEVCPKEIETPRKAIEKLRAEAAKRGLSLPAHKALASLIEKTGRSIERKEPT
FLERVPDVIEPEGEVRATVGFFVGCMFNGRVIQPALDAMEVLRKNGIRVIIPKDQVCCGSPLIRTGLCGFIPELQKRNID
AFVKAGVDTVLTICAGCGSTLKNDYDTPFRVVDITEFLCELGFEIPEKVEGTYTYHDPCHLLRGQGISEQPRILLNSVAE
KFVEMPARCCGAGGGVKSGLPEQAAAIGAVRAEMVKETGADYIVTVCPFCEFHLHQVTGLPVKNIATLMLEGYRKQEKKI
N

Sequences:

>Translated_481_residues
MKVTISRFDPATGEPPKFVEYDVSVKDSARVLNVLDAVHDTIDPEFAYRHCCRGGQCGSCAVRVNGEPVLACMQEAREGD
IIEPLELPRIRDLVTDIAPVMAQMAWMNSGCDCTPVTQQEIEEMKPLRECIECYSCISSCPAIAASDYKGPTIMRQEQRL
NLDPRDKGDRILEAIDKGLFSCTTCHKCVEVCPKEIETPRKAIEKLRAEAAKRGLSLPAHKALASLIEKTGRSIERKEPT
FLERVPDVIEPEGEVRATVGFFVGCMFNGRVIQPALDAMEVLRKNGIRVIIPKDQVCCGSPLIRTGLCGFIPELQKRNID
AFVKAGVDTVLTICAGCGSTLKNDYDTPFRVVDITEFLCELGFEIPEKVEGTYTYHDPCHLLRGQGISEQPRILLNSVAE
KFVEMPARCCGAGGGVKSGLPEQAAAIGAVRAEMVKETGADYIVTVCPFCEFHLHQVTGLPVKNIATLMLEGYRKQEKKI
N
>Mature_481_residues
MKVTISRFDPATGEPPKFVEYDVSVKDSARVLNVLDAVHDTIDPEFAYRHCCRGGQCGSCAVRVNGEPVLACMQEAREGD
IIEPLELPRIRDLVTDIAPVMAQMAWMNSGCDCTPVTQQEIEEMKPLRECIECYSCISSCPAIAASDYKGPTIMRQEQRL
NLDPRDKGDRILEAIDKGLFSCTTCHKCVEVCPKEIETPRKAIEKLRAEAAKRGLSLPAHKALASLIEKTGRSIERKEPT
FLERVPDVIEPEGEVRATVGFFVGCMFNGRVIQPALDAMEVLRKNGIRVIIPKDQVCCGSPLIRTGLCGFIPELQKRNID
AFVKAGVDTVLTICAGCGSTLKNDYDTPFRVVDITEFLCELGFEIPEKVEGTYTYHDPCHLLRGQGISEQPRILLNSVAE
KFVEMPARCCGAGGGVKSGLPEQAAAIGAVRAEMVKETGADYIVTVCPFCEFHLHQVTGLPVKNIATLMLEGYRKQEKKI
N

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI115387094, Length=232, Percent_Identity=31.0344827586207, Blast_Score=93, Evalue=5e-19,
Organism=Escherichia coli, GI1790596, Length=232, Percent_Identity=29.3103448275862, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786943, Length=239, Percent_Identity=29.2887029288703, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788576, Length=397, Percent_Identity=24.4332493702771, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786497, Length=208, Percent_Identity=28.8461538461538, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17533915, Length=229, Percent_Identity=31.8777292576419, Blast_Score=99, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6322987, Length=234, Percent_Identity=29.0598290598291, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24643156, Length=228, Percent_Identity=30.2631578947368, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI17137106, Length=230, Percent_Identity=30, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012675
- InterPro:   IPR004017
- InterPro:   IPR001041
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR004489 [H]

Pfam domain/function: PF02754 CCG; PF00111 Fer2 [H]

EC number: 1.3.99.1

Molecular weight: Translated: 52985; Mature: 52985

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
5.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTISRFDPATGEPPKFVEYDVSVKDSARVLNVLDAVHDTIDPEFAYRHCCRGGQCGSC
CEEEEECCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCE
AVRVNGEPVLACMQEAREGDIIEPLELPRIRDLVTDIAPVMAQMAWMNSGCDCTPVTQQE
EEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
IEEMKPLRECIECYSCISSCPAIAASDYKGPTIMRQEQRLNLDPRDKGDRILEAIDKGLF
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHH
SCTTCHKCVEVCPKEIETPRKAIEKLRAEAAKRGLSLPAHKALASLIEKTGRSIERKEPT
HHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCH
FLERVPDVIEPEGEVRATVGFFVGCMFNGRVIQPALDAMEVLRKNGIRVIIPKDQVCCGS
HHHHCCHHHCCCCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCEEEECCCCCCCCC
PLIRTGLCGFIPELQKRNIDAFVKAGVDTVLTICAGCGSTLKNDYDTPFRVVDITEFLCE
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEHHHHHHHHH
LGFEIPEKVEGTYTYHDPCHLLRGQGISEQPRILLNSVAEKFVEMPARCCGAGGGVKSGL
CCCCCCHHCCCCEEECCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PEQAAAIGAVRAEMVKETGADYIVTVCPFCEFHLHQVTGLPVKNIATLMLEGYRKQEKKI
CHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
N
C
>Mature Secondary Structure
MKVTISRFDPATGEPPKFVEYDVSVKDSARVLNVLDAVHDTIDPEFAYRHCCRGGQCGSC
CEEEEECCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCE
AVRVNGEPVLACMQEAREGDIIEPLELPRIRDLVTDIAPVMAQMAWMNSGCDCTPVTQQE
EEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
IEEMKPLRECIECYSCISSCPAIAASDYKGPTIMRQEQRLNLDPRDKGDRILEAIDKGLF
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHH
SCTTCHKCVEVCPKEIETPRKAIEKLRAEAAKRGLSLPAHKALASLIEKTGRSIERKEPT
HHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCH
FLERVPDVIEPEGEVRATVGFFVGCMFNGRVIQPALDAMEVLRKNGIRVIIPKDQVCCGS
HHHHCCHHHCCCCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCEEEECCCCCCCCC
PLIRTGLCGFIPELQKRNIDAFVKAGVDTVLTICAGCGSTLKNDYDTPFRVVDITEFLCE
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEHHHHHHHHH
LGFEIPEKVEGTYTYHDPCHLLRGQGISEQPRILLNSVAEKFVEMPARCCGAGGGVKSGL
CCCCCCHHCCCCEEECCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PEQAAAIGAVRAEMVKETGADYIVTVCPFCEFHLHQVTGLPVKNIATLMLEGYRKQEKKI
CHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.02 {succinate}} 0.3 {ferricyanide}} 0.4 {fumarate}} [C]

Substrates: succinate; acceptor

Specific reaction: succinate + acceptor = fumarate + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA