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The map label for this gene is truB [H]

Identifier: 124484901

GI number: 124484901

Start: 76186

End: 77034

Strand: Reverse

Name: truB [H]

Synonym: Mlab_0072

Alternate gene names: 124484901

Gene position: 77034-76186 (Counterclockwise)

Preceding gene: 124484902

Following gene: 124484899

Centisome position: 4.27

GC content: 52.77

Gene sequence:

>849_bases
ATGGGATATACCGGGATCATTTTGGTTGACAAGCCGAGAGGGCCCACGAGTCATCAGGTTGCTGCCTGGGTCGGCAAGAT
GCTGAAGTCGGACGTCGGTCATTCGGGAACACTCGATCCGATGGTCTCGGGGGTTCTTGTCGTAATGCTTGGTAAAGCAG
TGAGACTGGCTCCCCTCCTGCTTCAGCATGAAAAAGAGTACGTTGCATGCATGCGTCTCCATGCAGATATCCCGCGTGAA
CGCGTCGAAGAGGTCGTAAAGCAGTTCACCGGCAGAGTTTATCAGCGGCCGCCGAGACGGTCTGCAGTAAAACGTGCCCT
TCGCATTCGGGTGATCTACAGAATCGAACTCATCGATATGCAGGACAGACTGGTTCTCCTGAAAATCAGGTGCGAAGCGG
GAACTTATATCAGGTCGGTTTGCGTCCACATCGGCAATGTTCTCGGCTGCGGCGGTCAGATGATAGAACTGCGCAGAACA
AAATCCGGCGGATTTTCATGCGACGAGGCACACACTCTGCACGAGATTCGCGATGCAGTGGAACTGAAAGATGAAGCGGC
ACTTTCGGCGATGATTCTTCCTCCTGAGCAGGCAATTGGGGATATCCCAAAGATTGTGATTCGCGACAAAGCGGCAAAGG
CAGTTGCTAATGGAGCAATGCTCGCCGGAGTCGGCGCTATATCGGTGGAAAAATTTTCACGCGGGCAGAGTGTTGCTCTG
ATTACGCAAAGCGGAAAGCTCATCGCTCTTGCCGAATCTCTTTTTGATTCGGACAAAATCGATTGCGGGAAACCCGGTCT
GATTGCGAGATCAACACGCGTGTTCGCGACCCCCAACTCAGTAACTTAA

Upstream 100 bases:

>100_bases
AAATGATGTACGGAGGCGATGCCCCGGCGGTGATTGGCGACGGATCGATCGAAGGAACACTCAAAGCTCTCAACGCCTAT
CTTGTGACGGAGAGTCACTG

Downstream 100 bases:

>100_bases
TAAAGGCAGAGAACACATCTTTTATGCTAGTTTTTAGGCTGAGGTAGTCTAGCGGTAGGGCGCGGGCCTGGAAAGCCCGT
GAGGCGAAAGTCTCTCGGGA

Product: hypothetical protein

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MGYTGIILVDKPRGPTSHQVAAWVGKMLKSDVGHSGTLDPMVSGVLVVMLGKAVRLAPLLLQHEKEYVACMRLHADIPRE
RVEEVVKQFTGRVYQRPPRRSAVKRALRIRVIYRIELIDMQDRLVLLKIRCEAGTYIRSVCVHIGNVLGCGGQMIELRRT
KSGGFSCDEAHTLHEIRDAVELKDEAALSAMILPPEQAIGDIPKIVIRDKAAKAVANGAMLAGVGAISVEKFSRGQSVAL
ITQSGKLIALAESLFDSDKIDCGKPGLIARSTRVFATPNSVT

Sequences:

>Translated_282_residues
MGYTGIILVDKPRGPTSHQVAAWVGKMLKSDVGHSGTLDPMVSGVLVVMLGKAVRLAPLLLQHEKEYVACMRLHADIPRE
RVEEVVKQFTGRVYQRPPRRSAVKRALRIRVIYRIELIDMQDRLVLLKIRCEAGTYIRSVCVHIGNVLGCGGQMIELRRT
KSGGFSCDEAHTLHEIRDAVELKDEAALSAMILPPEQAIGDIPKIVIRDKAAKAVANGAMLAGVGAISVEKFSRGQSVAL
ITQSGKLIALAESLFDSDKIDCGKPGLIARSTRVFATPNSVT
>Mature_281_residues
GYTGIILVDKPRGPTSHQVAAWVGKMLKSDVGHSGTLDPMVSGVLVVMLGKAVRLAPLLLQHEKEYVACMRLHADIPRER
VEEVVKQFTGRVYQRPPRRSAVKRALRIRVIYRIELIDMQDRLVLLKIRCEAGTYIRSVCVHIGNVLGCGGQMIELRRTK
SGGFSCDEAHTLHEIRDAVELKDEAALSAMILPPEQAIGDIPKIVIRDKAAKAVANGAMLAGVGAISVEKFSRGQSVALI
TQSGKLIALAESLFDSDKIDCGKPGLIARSTRVFATPNSVT

Specific function: Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI215599015, Length=282, Percent_Identity=39.7163120567376, Blast_Score=174, Evalue=9e-44,
Organism=Homo sapiens, GI4503337, Length=282, Percent_Identity=39.7163120567376, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI21040257, Length=234, Percent_Identity=27.3504273504274, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI2367200, Length=290, Percent_Identity=26.2068965517241, Blast_Score=95, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17553978, Length=284, Percent_Identity=36.2676056338028, Blast_Score=188, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6323204, Length=258, Percent_Identity=41.4728682170543, Blast_Score=184, Evalue=2e-47,
Organism=Drosophila melanogaster, GI281364189, Length=282, Percent_Identity=39.3617021276596, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI281364187, Length=282, Percent_Identity=39.3617021276596, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI281364185, Length=282, Percent_Identity=39.3617021276596, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI281364183, Length=282, Percent_Identity=39.3617021276596, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI62471759, Length=282, Percent_Identity=39.3617021276596, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI17975520, Length=282, Percent_Identity=39.3617021276596, Blast_Score=186, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012960
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR004802
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR004521 [H]

Pfam domain/function: PF08068 DKCLD; PF01472 PUA; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 30698; Mature: 30566

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYTGIILVDKPRGPTSHQVAAWVGKMLKSDVGHSGTLDPMVSGVLVVMLGKAVRLAPLL
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LQHEKEYVACMRLHADIPRERVEEVVKQFTGRVYQRPPRRSAVKRALRIRVIYRIELIDM
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEEEEEECC
QDRLVLLKIRCEAGTYIRSVCVHIGNVLGCGGQMIELRRTKSGGFSCDEAHTLHEIRDAV
CCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
ELKDEAALSAMILPPEQAIGDIPKIVIRDKAAKAVANGAMLAGVGAISVEKFSRGQSVAL
HHHHHHHHEEEEECCHHHHCCCCCEEEHHHHHHHHHCCCCEECCCHHHHHHHCCCCEEEE
ITQSGKLIALAESLFDSDKIDCGKPGLIARSTRVFATPNSVT
EECCCCEEEEHHHHCCCCCCCCCCCCCEEECCEEEECCCCCC
>Mature Secondary Structure 
GYTGIILVDKPRGPTSHQVAAWVGKMLKSDVGHSGTLDPMVSGVLVVMLGKAVRLAPLL
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LQHEKEYVACMRLHADIPRERVEEVVKQFTGRVYQRPPRRSAVKRALRIRVIYRIELIDM
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEEEEEECC
QDRLVLLKIRCEAGTYIRSVCVHIGNVLGCGGQMIELRRTKSGGFSCDEAHTLHEIRDAV
CCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
ELKDEAALSAMILPPEQAIGDIPKIVIRDKAAKAVANGAMLAGVGAISVEKFSRGQSVAL
HHHHHHHHEEEEECCHHHHCCCCCEEEHHHHHHHHHCCCCEECCCHHHHHHHCCCCEEEE
ITQSGKLIALAESLFDSDKIDCGKPGLIARSTRVFATPNSVT
EECCCCEEEEHHHHCCCCCCCCCCCCCEEECCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA