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The map label for this gene is 124266186

Identifier: 124266186

GI number: 124266186

Start: 1054021

End: 1055094

Strand: Reverse

Name: 124266186

Synonym: Mpe_A0993

Alternate gene names: NA

Gene position: 1055094-1054021 (Counterclockwise)

Preceding gene: 124266190

Following gene: 124266176

Centisome position: 26.09

GC content: 72.16

Gene sequence:

>1074_bases
ATGCCGGTTTCGCACCCCAAGTTCCGCGCTGCCGCCGTCCAGGCTGCGCCGGTGTTCCTGGATCTCGACGCGTCGATCGA
CAAGGCCGTGGGCCTGATCGCCGAGGCCGCCACGCAGGGCGCGCAGCTCGTGGCCTTTCCGGAAACCTGGCTGCCCGGCT
ACCCGTGGTTCATCTGGCTCGATTCGCCGGCCTGGGCGCTGCAGTTCATCCAGCGCTACCACGATCACTCGCTGGTCGAC
GGCTCGCCGCAGGCCGATCGCATCGCCCAGGCGGCCCGCGACCACGCCATCATGGTCGTGATGGGCCACAGCGAGAAGGC
CGGCGGCAGCCTCTACATGGGCCAGTGGATCATCGACGCCGAGGGCCGCACGGTGGCGCGGCGCCGCAAGCTCAAGCCCA
CGCACGTGGAGCGCACCGTGTTCGGCGAGGGCGACGGCAGCGACCTCGGCGTGCACGACACCGCGCTCGGACGCATCGGC
GCGCTGTGCTGCTGGGAGCATCTGCAACCGCTGTCGAAGTACGCGATGTACGCGCAGGACGAGCAGGTGCACATCGCCGC
CTGGCCCAGCTTCTCGCTCTACCGTGGCGGCGCCCATGCGCTGGGCGCGGAGGTGAACACCGCCGCCAGCCGCATCTACG
CGGTGGAAGGCCAGTGCTTCGTGGTCGCCCCCTGCGCCACGGTCTCGAAGGAAATGGTGGCGATGCTGTGCGGCGACGAC
CCGACGAAGCGACAGCTGCTGCTCGAAGGCGGTGGCTATGCGGGCATCTACGCGCCCGACGGCCGCCTGCTGACCGAGCC
GCTGCCGGAGCACACCGAGGGGCTCGTCCACGCCGAGCTCGACCTGGGCCTGATCACGCTGGCCAAGGCGGCGGCCGATC
CGGCCGGCCACTACTCGCGGCCCGACGTGACGCGCCTGCTGCTCGACAAGACACCGCGCGACCGCGTGGTGCTGGCGCCG
CAGCGCCCTCCCGGCGCGTCGGCAGGCGCCGCCGAGGCCGCACCACCGCCGTCCGGCACATCGCCCGGTGACCCGGCCGC
TGCCGGCCCGTCGACCGCGCGCCGCACGGCTTGA

Upstream 100 bases:

>100_bases
GCGCGCTGCGTGCTGCGGTCAAGTACCGCGCCTCGTCCAGGCAAGACGCCGGGACCGATCCGTCGCATGCTCCGCTCCAT
TGCACGACAGGAGCCTCGCC

Downstream 100 bases:

>100_bases
GCGTCGGGCGATCGCCGCGTTCACCACGCGCGCTCCTCGCGGCTCAGCCGCCGGGGGCCGCCGGCGGGGCTGCCGCCTGC
ACGGCCGGCAGCCCGCCGAG

Product: aliphatic nitrilase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MPVSHPKFRAAAVQAAPVFLDLDASIDKAVGLIAEAATQGAQLVAFPETWLPGYPWFIWLDSPAWALQFIQRYHDHSLVD
GSPQADRIAQAARDHAIMVVMGHSEKAGGSLYMGQWIIDAEGRTVARRRKLKPTHVERTVFGEGDGSDLGVHDTALGRIG
ALCCWEHLQPLSKYAMYAQDEQVHIAAWPSFSLYRGGAHALGAEVNTAASRIYAVEGQCFVVAPCATVSKEMVAMLCGDD
PTKRQLLLEGGGYAGIYAPDGRLLTEPLPEHTEGLVHAELDLGLITLAKAAADPAGHYSRPDVTRLLLDKTPRDRVVLAP
QRPPGASAGAAEAAPPPSGTSPGDPAAAGPSTARRTA

Sequences:

>Translated_357_residues
MPVSHPKFRAAAVQAAPVFLDLDASIDKAVGLIAEAATQGAQLVAFPETWLPGYPWFIWLDSPAWALQFIQRYHDHSLVD
GSPQADRIAQAARDHAIMVVMGHSEKAGGSLYMGQWIIDAEGRTVARRRKLKPTHVERTVFGEGDGSDLGVHDTALGRIG
ALCCWEHLQPLSKYAMYAQDEQVHIAAWPSFSLYRGGAHALGAEVNTAASRIYAVEGQCFVVAPCATVSKEMVAMLCGDD
PTKRQLLLEGGGYAGIYAPDGRLLTEPLPEHTEGLVHAELDLGLITLAKAAADPAGHYSRPDVTRLLLDKTPRDRVVLAP
QRPPGASAGAAEAAPPPSGTSPGDPAAAGPSTARRTA
>Mature_356_residues
PVSHPKFRAAAVQAAPVFLDLDASIDKAVGLIAEAATQGAQLVAFPETWLPGYPWFIWLDSPAWALQFIQRYHDHSLVDG
SPQADRIAQAARDHAIMVVMGHSEKAGGSLYMGQWIIDAEGRTVARRRKLKPTHVERTVFGEGDGSDLGVHDTALGRIGA
LCCWEHLQPLSKYAMYAQDEQVHIAAWPSFSLYRGGAHALGAEVNTAASRIYAVEGQCFVVAPCATVSKEMVAMLCGDDP
TKRQLLLEGGGYAGIYAPDGRLLTEPLPEHTEGLVHAELDLGLITLAKAAADPAGHYSRPDVTRLLLDKTPRDRVVLAPQ
RPPGASAGAAEAAPPPSGTSPGDPAAAGPSTARRTA

Specific function: Acts on aliphatic nitriles such as acrylonitrile, crotononitrile and glutaronitrile [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17557111, Length=320, Percent_Identity=31.25, Blast_Score=150, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6322027, Length=192, Percent_Identity=30.7291666666667, Blast_Score=86, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000132
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.5.7 [H]

Molecular weight: Translated: 37969; Mature: 37838

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00920 NITRIL_CHT_1 ; PS00921 NITRIL_CHT_2 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVSHPKFRAAAVQAAPVFLDLDASIDKAVGLIAEAATQGAQLVAFPETWLPGYPWFIWL
CCCCCCCHHHHHEEECEEEEECCCCHHHHHHHHHHHHCCCCEEEEECHHHCCCCCEEEEE
DSPAWALQFIQRYHDHSLVDGSPQADRIAQAARDHAIMVVMGHSEKAGGSLYMGQWIIDA
CCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEEEEEC
EGRTVARRRKLKPTHVERTVFGEGDGSDLGVHDTALGRIGALCCWEHLQPLSKYAMYAQD
CCCCHHHHHCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EQVHIAAWPSFSLYRGGAHALGAEVNTAASRIYAVEGQCFVVAPCATVSKEMVAMLCGDD
CEEEEEECCCCCEECCCCHHCCCCHHHHHHEEEEECCCEEEEECHHHHHHHHHHHHCCCC
PTKRQLLLEGGGYAGIYAPDGRLLTEPLPEHTEGLVHAELDLGLITLAKAAADPAGHYSR
CCCEEEEEECCCEEEEECCCCCEECCCCCCHHCCEEEEEECCCHHHHHHHHCCCCCCCCC
PDVTRLLLDKTPRDRVVLAPQRPPGASAGAAEAAPPPSGTSPGDPAAAGPSTARRTA
CHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PVSHPKFRAAAVQAAPVFLDLDASIDKAVGLIAEAATQGAQLVAFPETWLPGYPWFIWL
CCCCCCHHHHHEEECEEEEECCCCHHHHHHHHHHHHCCCCEEEEECHHHCCCCCEEEEE
DSPAWALQFIQRYHDHSLVDGSPQADRIAQAARDHAIMVVMGHSEKAGGSLYMGQWIIDA
CCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEEEEEC
EGRTVARRRKLKPTHVERTVFGEGDGSDLGVHDTALGRIGALCCWEHLQPLSKYAMYAQD
CCCCHHHHHCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EQVHIAAWPSFSLYRGGAHALGAEVNTAASRIYAVEGQCFVVAPCATVSKEMVAMLCGDD
CEEEEEECCCCCEECCCCHHCCCCHHHHHHEEEEECCCEEEEECHHHHHHHHHHHHCCCC
PTKRQLLLEGGGYAGIYAPDGRLLTEPLPEHTEGLVHAELDLGLITLAKAAADPAGHYSR
CCCEEEEEECCCEEEEECCCCCEECCCCCCHHCCEEEEEECCCHHHHHHHHCCCCCCCCC
PDVTRLLLDKTPRDRVVLAPQRPPGASAGAAEAAPPPSGTSPGDPAAAGPSTARRTA
CHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1390687 [H]