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The map label for this gene is lutR [H]

Identifier: 124266164

GI number: 124266164

Start: 1034478

End: 1035224

Strand: Direct

Name: lutR [H]

Synonym: Mpe_A0971

Alternate gene names: 124266164

Gene position: 1034478-1035224 (Clockwise)

Preceding gene: 124266163

Following gene: 124266165

Centisome position: 25.58

GC content: 74.03

Gene sequence:

>747_bases
ATGGACATGCCTTTCGCGCCTCGCCGCCGCCCCCGCACGCTCGCGCTCGAGCTGGTCGATGCGTTGGGTGACCGCATCCG
CGACGGCCGCCTGGCGCCCGGGGCCAAGCTGCCCACCGAGGCGGCGATCATGGCGGAGTTCGGTGTCAGCCGCACCGTGG
TGCGCGAGGCGATCTCCAAGCTCCAGGCAGGCGGGCTGGTGGCCACCCGGCATGGTGTCGGCACCTTCGTGGTCGGGTTC
GGCGATGCGGCGCCGTTCCGCATCGCACCGGAGCAGTTCGCGACGCTGCGCGACGTGATCGCGGTGCTGGAGCTGCGTAT
TGGCGTCGAGACCGAGGCGGCCGGTCTCGCCGCGCAGCGCCGCAGCGCCGACAACCTGGCCACCATGCGCAGCGCGCTGG
ATGCCTTCGCCGCCGCGGTGGAGGCGGGGCGCGACGCGGTGGGCGCCGACTTCCAGTTCCATCTGGAGGTGGCGCGCGCG
ACCCAGAACCACCATTTCGCCGAGCTGATGGGCACGCTGGGCGCGATGATCATCCCGCGGGCGCGGCTCGATGTGCCGCA
GGCGCAGGGCGCCGAGCAACGCGACTACCTGCGCCGCGTGAACGGCGAGCACGAGAGCATCTACGACGCCATCGCCAGCC
AGGATGCCGAGGCGGCTCGCGCCGCGATGCGCACCCACCTGGCCAACAGCCGCGAGCGCCGCCGCCGCGCCGGCGCGGCG
GCGCAGGCGCGCCAGCCCCGGGCCTGA

Upstream 100 bases:

>100_bases
ATGATGTCGGCCCGATCGCTTGCTTTTGCGTGGCGGTTGTCATACAACTGCTGCCCATCCACCCCGGCCCCCGAGGCCGC
CATCACCGGGTCCTCCCGAC

Downstream 100 bases:

>100_bases
GCGGCGGCGGGCGCAAGCGGACCGATACCGGGCAATCCCCAGTGGTCCGTCATCCTTCATGTTGTACGATGACGTACAAC
TAATGAGAGGCTTGTCGCCG

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MDMPFAPRRRPRTLALELVDALGDRIRDGRLAPGAKLPTEAAIMAEFGVSRTVVREAISKLQAGGLVATRHGVGTFVVGF
GDAAPFRIAPEQFATLRDVIAVLELRIGVETEAAGLAAQRRSADNLATMRSALDAFAAAVEAGRDAVGADFQFHLEVARA
TQNHHFAELMGTLGAMIIPRARLDVPQAQGAEQRDYLRRVNGEHESIYDAIASQDAEAARAAMRTHLANSRERRRRAGAA
AQARQPRA

Sequences:

>Translated_248_residues
MDMPFAPRRRPRTLALELVDALGDRIRDGRLAPGAKLPTEAAIMAEFGVSRTVVREAISKLQAGGLVATRHGVGTFVVGF
GDAAPFRIAPEQFATLRDVIAVLELRIGVETEAAGLAAQRRSADNLATMRSALDAFAAAVEAGRDAVGADFQFHLEVARA
TQNHHFAELMGTLGAMIIPRARLDVPQAQGAEQRDYLRRVNGEHESIYDAIASQDAEAARAAMRTHLANSRERRRRAGAA
AQARQPRA
>Mature_248_residues
MDMPFAPRRRPRTLALELVDALGDRIRDGRLAPGAKLPTEAAIMAEFGVSRTVVREAISKLQAGGLVATRHGVGTFVVGF
GDAAPFRIAPEQFATLRDVIAVLELRIGVETEAAGLAAQRRSADNLATMRSALDAFAAAVEAGRDAVGADFQFHLEVARA
TQNHHFAELMGTLGAMIIPRARLDVPQAQGAEQRDYLRRVNGEHESIYDAIASQDAEAARAAMRTHLANSRERRRRAGAA
AQARQPRA

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082208, Length=238, Percent_Identity=30.2521008403361, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1790032, Length=231, Percent_Identity=31.1688311688312, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI48994955, Length=231, Percent_Identity=29.004329004329, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790780, Length=221, Percent_Identity=30.7692307692308, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786303, Length=235, Percent_Identity=31.063829787234, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 26738; Mature: 26738

Theoretical pI: Translated: 10.71; Mature: 10.71

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMPFAPRRRPRTLALELVDALGDRIRDGRLAPGAKLPTEAAIMAEFGVSRTVVREAISK
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHH
LQAGGLVATRHGVGTFVVGFGDAAPFRIAPEQFATLRDVIAVLELRIGVETEAAGLAAQR
HHHCCEEEEECCCEEEEEECCCCCCCEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RSADNLATMRSALDAFAAAVEAGRDAVGADFQFHLEVARATQNHHFAELMGTLGAMIIPR
HCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCH
ARLDVPQAQGAEQRDYLRRVNGEHESIYDAIASQDAEAARAAMRTHLANSRERRRRAGAA
HHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCH
AQARQPRA
HHCCCCCC
>Mature Secondary Structure
MDMPFAPRRRPRTLALELVDALGDRIRDGRLAPGAKLPTEAAIMAEFGVSRTVVREAISK
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHH
LQAGGLVATRHGVGTFVVGFGDAAPFRIAPEQFATLRDVIAVLELRIGVETEAAGLAAQR
HHHCCEEEEECCCEEEEEECCCCCCCEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RSADNLATMRSALDAFAAAVEAGRDAVGADFQFHLEVARATQNHHFAELMGTLGAMIIPR
HCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCH
ARLDVPQAQGAEQRDYLRRVNGEHESIYDAIASQDAEAARAAMRTHLANSRERRRRAGAA
HHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCH
AQARQPRA
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]