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The map label for this gene is gudD [H]

Identifier: 124266163

GI number: 124266163

Start: 1033006

End: 1034358

Strand: Direct

Name: gudD [H]

Synonym: Mpe_A0970

Alternate gene names: 124266163

Gene position: 1033006-1034358 (Clockwise)

Preceding gene: 124266162

Following gene: 124266164

Centisome position: 25.54

GC content: 71.99

Gene sequence:

>1353_bases
ATGCTCAACACCTCCTTGTCTTCCACGCCGCGTGTCACCGCGATGCGCGTCATCCCGGTCGCGGGGCGCGACTCGATGCT
GATGAACCTGAGCGGCGCGCACGGGCCGTTCTTCACCCGCAACCTCGTGATCCTGAGCGACAACGCCGGGCGGAGCGGCG
TCGGCGAGGTGCCGGGCGGCGAGAAGATCCGCCAGACGCTGGAGGACGCGCGCGAGTGGGTCGTCGGCCAACCGGTCGGC
GACGTGCAGCGCGTGCTGCGTACGGTGCGCGAGCGCTTCGCCGACCGCGACGCCGGCGGCCGCGGGCTGCAGACCTTCGA
CCTGCGCACCACCATCCACGTCGTGACGGCCGTCGAGTCGGCGCTGCTCGACCTGCTGGGCCAGCACCTCGAGCTGCCGG
TCGCGGCGCTGCTCGGCGAGGGCCAGCAGCGCACGTCGGTCGAGATGCTCGGCTACCTGTTCTTCGTCGGTGACCGCACG
AAGACCGACCTGGCCTACGCGGGCCCGGCCGACGAGGCGACCGACGCCGACGACTGGCAGCGCCTGCGCCACGAGCCGGC
GATGACGCCCGAGGAGGTGGTGCGGCTGGCGGAGGCGGCGCGCGCGCGCTACGGCTTCAACGACTTCAAGCTCAAGGGCG
GCGTGCTGGCCGGCGACGCCGAGGTCGACGCGGTCACCGCGATCCACGAGCGCTTCCCCGACGCCCGCGTGACGCTCGAT
CCCAACGGCGGCTGGCTGCTCAAGGACGCGATCCGCCTCGGCCAGCGCATGCGCGGCGTGGTGGCCTACGCCGAGGACCC
CTGCGGTGCGGAAGAGGGCTACTCCGGCCGCGAGGTGATGGCCGAGTTCCGCCGCGCCACCGGCCTGCCGACCGCGACCA
ACATGGTCGCCACCGACTGGCGCCAGCTCACGCACGCGCTGTCGCTGCAGTCGGTCGACATCCCGCTCGCCGATCCGCAT
TTCTGGACGATGGCCGGCTCGGTGCGCGTGGCGCAGACCTGCCGCGACTGGGGCCTGACCTGGGGCTCGCACTCGAACAA
CCACTTCGACGTCTCGCTGGCGATGTTCACCCACGTCGGTGCCGCGGCGCCGGGCCGCGTGACCGCGATCGACACGCACT
GGATCTGGCAGGACGGACAGCGCCTGACGAAGGAGCCGCTGCAGATCGTCGGTGGCCACGTGCGGGTGCCGCAGCGGCCG
GGCCTGGGCATCGAGCTCGACATGGCCGAGGTCGAGAAGGCCCACCGCCTCTACCTCGAGCACGGCCTGGGCGCGCGCGA
CGACGCGGTGGCGATGCAGCACCTGATCCCGAACTGGCGCTTCGATCCAAAACGGCCCTGCATGCTGCGCTGA

Upstream 100 bases:

>100_bases
GCCTTCGGCGCTCCCCCTCGAGGGGGCGCCGCTGGCGGACCGGCGGAGCCGGATCCGCGGCGGCTGCTGGATCAGACCGG
TTCGAACTGCTAGGACTTCC

Downstream 100 bases:

>100_bases
ACCGGTGGCCGTCTCGGCGATGATGTCGGCCCGATCGCTTGCTTTTGCGTGGCGGTTGTCATACAACTGCTGCCCATCCA
CCCCGGCCCCCGAGGCCGCC

Product: D-glucarate dehydratase

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MLNTSLSSTPRVTAMRVIPVAGRDSMLMNLSGAHGPFFTRNLVILSDNAGRSGVGEVPGGEKIRQTLEDAREWVVGQPVG
DVQRVLRTVRERFADRDAGGRGLQTFDLRTTIHVVTAVESALLDLLGQHLELPVAALLGEGQQRTSVEMLGYLFFVGDRT
KTDLAYAGPADEATDADDWQRLRHEPAMTPEEVVRLAEAARARYGFNDFKLKGGVLAGDAEVDAVTAIHERFPDARVTLD
PNGGWLLKDAIRLGQRMRGVVAYAEDPCGAEEGYSGREVMAEFRRATGLPTATNMVATDWRQLTHALSLQSVDIPLADPH
FWTMAGSVRVAQTCRDWGLTWGSHSNNHFDVSLAMFTHVGAAAPGRVTAIDTHWIWQDGQRLTKEPLQIVGGHVRVPQRP
GLGIELDMAEVEKAHRLYLEHGLGARDDAVAMQHLIPNWRFDPKRPCMLR

Sequences:

>Translated_450_residues
MLNTSLSSTPRVTAMRVIPVAGRDSMLMNLSGAHGPFFTRNLVILSDNAGRSGVGEVPGGEKIRQTLEDAREWVVGQPVG
DVQRVLRTVRERFADRDAGGRGLQTFDLRTTIHVVTAVESALLDLLGQHLELPVAALLGEGQQRTSVEMLGYLFFVGDRT
KTDLAYAGPADEATDADDWQRLRHEPAMTPEEVVRLAEAARARYGFNDFKLKGGVLAGDAEVDAVTAIHERFPDARVTLD
PNGGWLLKDAIRLGQRMRGVVAYAEDPCGAEEGYSGREVMAEFRRATGLPTATNMVATDWRQLTHALSLQSVDIPLADPH
FWTMAGSVRVAQTCRDWGLTWGSHSNNHFDVSLAMFTHVGAAAPGRVTAIDTHWIWQDGQRLTKEPLQIVGGHVRVPQRP
GLGIELDMAEVEKAHRLYLEHGLGARDDAVAMQHLIPNWRFDPKRPCMLR
>Mature_450_residues
MLNTSLSSTPRVTAMRVIPVAGRDSMLMNLSGAHGPFFTRNLVILSDNAGRSGVGEVPGGEKIRQTLEDAREWVVGQPVG
DVQRVLRTVRERFADRDAGGRGLQTFDLRTTIHVVTAVESALLDLLGQHLELPVAALLGEGQQRTSVEMLGYLFFVGDRT
KTDLAYAGPADEATDADDWQRLRHEPAMTPEEVVRLAEAARARYGFNDFKLKGGVLAGDAEVDAVTAIHERFPDARVTLD
PNGGWLLKDAIRLGQRMRGVVAYAEDPCGAEEGYSGREVMAEFRRATGLPTATNMVATDWRQLTHALSLQSVDIPLADPH
FWTMAGSVRVAQTCRDWGLTWGSHSNNHFDVSLAMFTHVGAAAPGRVTAIDTHWIWQDGQRLTKEPLQIVGGHVRVPQRP
GLGIELDMAEVEKAHRLYLEHGLGARDDAVAMQHLIPNWRFDPKRPCMLR

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc). Also acts on L-idarate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=448, Percent_Identity=71.875, Blast_Score=685, Evalue=0.0,
Organism=Escherichia coli, GI1789151, Length=449, Percent_Identity=60.3563474387528, Blast_Score=538, Evalue=1e-154,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017653
- InterPro:   IPR013342
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 49555; Mature: 49555

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNTSLSSTPRVTAMRVIPVAGRDSMLMNLSGAHGPFFTRNLVILSDNAGRSGVGEVPGG
CCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCEECEEEEEECCCCCCCCCCCCCH
EKIRQTLEDAREWVVGQPVGDVQRVLRTVRERFADRDAGGRGLQTFDLRTTIHVVTAVES
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHH
ALLDLLGQHLELPVAALLGEGQQRTSVEMLGYLFFVGDRTKTDLAYAGPADEATDADDWQ
HHHHHHHHHHCCCHHHHHCCCCCHHHHHHHEEHEEECCCCCCCEEECCCCCCCCCHHHHH
RLRHEPAMTPEEVVRLAEAARARYGFNDFKLKGGVLAGDAEVDAVTAIHERFPDARVTLD
HHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCEEECCCCHHHHHHHHHHCCCCEEEEC
PNGGWLLKDAIRLGQRMRGVVAYAEDPCGAEEGYSGREVMAEFRRATGLPTATNMVATDW
CCCCEEHHHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
RQLTHALSLQSVDIPLADPHFWTMAGSVRVAQTCRDWGLTWGSHSNNHFDVSLAMFTHVG
HHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHC
AAAPGRVTAIDTHWIWQDGQRLTKEPLQIVGGHVRVPQRPGLGIELDMAEVEKAHRLYLE
CCCCCCEEEEEEEEEECCCHHHHHHHHHHHCCCEECCCCCCCCEEECHHHHHHHHHHHHH
HGLGARDDAVAMQHLIPNWRFDPKRPCMLR
HCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLNTSLSSTPRVTAMRVIPVAGRDSMLMNLSGAHGPFFTRNLVILSDNAGRSGVGEVPGG
CCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCEECEEEEEECCCCCCCCCCCCCH
EKIRQTLEDAREWVVGQPVGDVQRVLRTVRERFADRDAGGRGLQTFDLRTTIHVVTAVES
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHH
ALLDLLGQHLELPVAALLGEGQQRTSVEMLGYLFFVGDRTKTDLAYAGPADEATDADDWQ
HHHHHHHHHHCCCHHHHHCCCCCHHHHHHHEEHEEECCCCCCCEEECCCCCCCCCHHHHH
RLRHEPAMTPEEVVRLAEAARARYGFNDFKLKGGVLAGDAEVDAVTAIHERFPDARVTLD
HHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCEEECCCCHHHHHHHHHHCCCCEEEEC
PNGGWLLKDAIRLGQRMRGVVAYAEDPCGAEEGYSGREVMAEFRRATGLPTATNMVATDW
CCCCEEHHHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
RQLTHALSLQSVDIPLADPHFWTMAGSVRVAQTCRDWGLTWGSHSNNHFDVSLAMFTHVG
HHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHC
AAAPGRVTAIDTHWIWQDGQRLTKEPLQIVGGHVRVPQRPGLGIELDMAEVEKAHRLYLE
CCCCCCEEEEEEEEEECCCHHHHHHHHHHHCCCEECCCCCCCCEEECHHHHHHHHHHHHH
HGLGARDDAVAMQHLIPNWRFDPKRPCMLR
HCCCCCHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]