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The map label for this gene is mscL

Identifier: 124266041

GI number: 124266041

Start: 885330

End: 885743

Strand: Reverse

Name: mscL

Synonym: Mpe_A0848

Alternate gene names: 124266041

Gene position: 885743-885330 (Counterclockwise)

Preceding gene: 124266049

Following gene: 124266040

Centisome position: 21.9

GC content: 61.59

Gene sequence:

>414_bases
ATGTCGTTCTTCAGCGAGTTCAAGGAGTTTGCCGTCAAGGGCAATGTGATCGACCTGGCCGTTGGCGTGATCATCGGCGG
CGCCTTCGGCAAGATCGTCGACTCGATCGTCGGCGACCTGATCATGCCGATCGTCAGCAAGCTGTTCGGCGGCCTCGACT
TCTCGAATTACTACGTCGGACTGGCGGGGCAGGCCGCCGGACTGCCGCTCGCGGAGGCCAAGAAGGCCGGCGCGGTCTTC
GCCTATGGCAACTTCATCACGGTGGCGCTGAACTTCGCGATCCTCGCCTTCATCATTTTCCTGATGATCAAGCAGATCAA
CCGACTGAAGAAGGACGAACCGGCCGCCCCGCCTGCGCCCCCCGCCGAGGACATCGTGCTGTTGCGCGAGATCCGCGATG
CCCTGAAGAAGTGA

Upstream 100 bases:

>100_bases
GCCAGCTCGGCTTGACTTGCCGCAAACCTCGGGAATACTCGCTCACGACAGGCGCCGGACACCCCGGTCGACCATCATTA
CTACCTTCAGGAGGCTGTGC

Downstream 100 bases:

>100_bases
CCCGCCTGCGGGCGCGCTGCCGGGGCACCGGCCCGCCCGCCTCCTTGCCGACCCTGTGAACGCCGCCCCCAACGTCCAGG
CCGCATTGAACGCGGCATGC

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 137; Mature: 136

Protein sequence:

>137_residues
MSFFSEFKEFAVKGNVIDLAVGVIIGGAFGKIVDSIVGDLIMPIVSKLFGGLDFSNYYVGLAGQAAGLPLAEAKKAGAVF
AYGNFITVALNFAILAFIIFLMIKQINRLKKDEPAAPPAPPAEDIVLLREIRDALKK

Sequences:

>Translated_137_residues
MSFFSEFKEFAVKGNVIDLAVGVIIGGAFGKIVDSIVGDLIMPIVSKLFGGLDFSNYYVGLAGQAAGLPLAEAKKAGAVF
AYGNFITVALNFAILAFIIFLMIKQINRLKKDEPAAPPAPPAEDIVLLREIRDALKK
>Mature_136_residues
SFFSEFKEFAVKGNVIDLAVGVIIGGAFGKIVDSIVGDLIMPIVSKLFGGLDFSNYYVGLAGQAAGLPLAEAKKAGAVFA
YGNFITVALNFAILAFIIFLMIKQINRLKKDEPAAPPAPPAEDIVLLREIRDALKK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=139, Percent_Identity=53.2374100719424, Blast_Score=127, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_METPP (A2SE21)

Other databases:

- EMBL:   CP000555
- RefSeq:   YP_001020045.1
- ProteinModelPortal:   A2SE21
- SMR:   A2SE21
- STRING:   A2SE21
- GeneID:   4787141
- GenomeReviews:   CP000555_GR
- KEGG:   mpt:Mpe_A0848
- NMPDR:   fig|279263.3.peg.2027
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- PhylomeDB:   A2SE21
- ProtClustDB:   PRK13952
- BioCyc:   MPET420662:MPE_A0848-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 14634; Mature: 14502

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x252beaa0)-; HASH(0x24fcaa30)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFFSEFKEFAVKGNVIDLAVGVIIGGAFGKIVDSIVGDLIMPIVSKLFGGLDFSNYYVG
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
LAGQAAGLPLAEAKKAGAVFAYGNFITVALNFAILAFIIFLMIKQINRLKKDEPAAPPAP
CCCCCCCCCHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PAEDIVLLREIRDALKK
CHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
SFFSEFKEFAVKGNVIDLAVGVIIGGAFGKIVDSIVGDLIMPIVSKLFGGLDFSNYYVG
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
LAGQAAGLPLAEAKKAGAVFAYGNFITVALNFAILAFIIFLMIKQINRLKKDEPAAPPAP
CCCCCCCCCHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PAEDIVLLREIRDALKK
CHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA