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The map label for this gene is hisH [H]

Identifier: 124266027

GI number: 124266027

Start: 874141

End: 874797

Strand: Direct

Name: hisH [H]

Synonym: Mpe_A0834

Alternate gene names: 124266027

Gene position: 874141-874797 (Clockwise)

Preceding gene: 124266026

Following gene: 124266028

Centisome position: 21.61

GC content: 70.02

Gene sequence:

>657_bases
ATGAGCCGTCGCGTGGCGGTCATCGACTACGGCATGGGCAACCTGCGCTCGGTGTCGCAGGCCGTGCTGCACGCGGCCGC
CGGGACCGGCTTCGAGGTGGTTGTGACGGCCGAGCCCGAGGTGGTGCGCGCTGCCGAACGCGTGGTGCTGCCCGGCCAGG
GCGCGATGCGCGACTGCATGCGCGAGCTGCGAGAGGCGCACGGGGGCGCCCTGCGGGCCGCCGTGCTCGAGGCCGCCGCC
GACAAGCCGCTGATGGGCGTGTGCGTCGGCATGCAGATGCTGCTCGACCACAGCGAGGAACAGGACACGCCCGGCCTGGG
CCTGATCCCGGGCCGGGTGCGGCGCTTCCAGCTCGAAGGCCGACTCCAGCCCGACGGCAGCCGCTACAAGGTGCCGCAGA
TGGGCTGGAACCAGGTGCTGCAGGCCCGTCCGCACCCGGTGTGGGCCGGCGTGCCCGATGCCAGCTGGTTCTATTTCGTG
CACAGCTTCTATGCGCAGCCGGCCGATCCGGCGCACAGCGTTGGTGAAACCGACTATGGCGCGCGCTTTACCAGTGCGGT
GGCACGCGATAATATTTTTGCCACCCAGTTCCACCCCGAGAAGAGCGCTGCACACGGCCTGGCCCTGTACCGCAACTTCC
TCGGCTGGTGCCCCTGA

Upstream 100 bases:

>100_bases
GAGACGGTCTTCAAGGCCTTCGCCCGAGCGCTGCGCATGGCGCTGGAGCGCGACCCGCGCATGGCCGGCGTCATCCCTTC
CACCAAGGGCAGCCTCTGAC

Downstream 100 bases:

>100_bases
CCCCCCATCCCCCTGACAGCCATGTTGCTGATCCCTGCCATCGACCTGAAAGACGGCCGCTGCGTGCGCCTGAAGCAAGG
CGACATGAATGTGTCCACCA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MSRRVAVIDYGMGNLRSVSQAVLHAAAGTGFEVVVTAEPEVVRAAERVVLPGQGAMRDCMRELREAHGGALRAAVLEAAA
DKPLMGVCVGMQMLLDHSEEQDTPGLGLIPGRVRRFQLEGRLQPDGSRYKVPQMGWNQVLQARPHPVWAGVPDASWFYFV
HSFYAQPADPAHSVGETDYGARFTSAVARDNIFATQFHPEKSAAHGLALYRNFLGWCP

Sequences:

>Translated_218_residues
MSRRVAVIDYGMGNLRSVSQAVLHAAAGTGFEVVVTAEPEVVRAAERVVLPGQGAMRDCMRELREAHGGALRAAVLEAAA
DKPLMGVCVGMQMLLDHSEEQDTPGLGLIPGRVRRFQLEGRLQPDGSRYKVPQMGWNQVLQARPHPVWAGVPDASWFYFV
HSFYAQPADPAHSVGETDYGARFTSAVARDNIFATQFHPEKSAAHGLALYRNFLGWCP
>Mature_217_residues
SRRVAVIDYGMGNLRSVSQAVLHAAAGTGFEVVVTAEPEVVRAAERVVLPGQGAMRDCMRELREAHGGALRAAVLEAAAD
KPLMGVCVGMQMLLDHSEEQDTPGLGLIPGRVRRFQLEGRLQPDGSRYKVPQMGWNQVLQARPHPVWAGVPDASWFYFVH
SFYAQPADPAHSVGETDYGARFTSAVARDNIFATQFHPEKSAAHGLALYRNFLGWCP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=212, Percent_Identity=36.7924528301887, Blast_Score=130, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6319725, Length=223, Percent_Identity=34.0807174887892, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 23825; Mature: 23694

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRVAVIDYGMGNLRSVSQAVLHAAAGTGFEVVVTAEPEVVRAAERVVLPGQGAMRDCM
CCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHEECCCCHHHHHHH
RELREAHGGALRAAVLEAAADKPLMGVCVGMQMLLDHSEEQDTPGLGLIPGRVRRFQLEG
HHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHEEEEECC
RLQPDGSRYKVPQMGWNQVLQARPHPVWAGVPDASWFYFVHSFYAQPADPAHSVGETDYG
CCCCCCCEEECCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHCCCCCCC
ARFTSAVARDNIFATQFHPEKSAAHGLALYRNFLGWCP
HHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRRVAVIDYGMGNLRSVSQAVLHAAAGTGFEVVVTAEPEVVRAAERVVLPGQGAMRDCM
CCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHEECCCCHHHHHHH
RELREAHGGALRAAVLEAAADKPLMGVCVGMQMLLDHSEEQDTPGLGLIPGRVRRFQLEG
HHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHEEEEECC
RLQPDGSRYKVPQMGWNQVLQARPHPVWAGVPDASWFYFVHSFYAQPADPAHSVGETDYG
CCCCCCCEEECCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHCCCCCCC
ARFTSAVARDNIFATQFHPEKSAAHGLALYRNFLGWCP
HHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12754231 [H]