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The map label for this gene is tbuA1 [H]

Identifier: 124266007

GI number: 124266007

Start: 856061

End: 857560

Strand: Direct

Name: tbuA1 [H]

Synonym: Mpe_A0814

Alternate gene names: 124266007

Gene position: 856061-857560 (Clockwise)

Preceding gene: 124266003

Following gene: 124266008

Centisome position: 21.17

GC content: 60.93

Gene sequence:

>1500_bases
ATGGCGCTACTTGAGCGGATGGATTGGTACGACCTCGCGCGGACCACCAATTGGACGCCGACCTACGTCAGCGAGGCAGA
GCTGTTTCCGACCGAGATGTCGGGGGACATGGGAATCCCGATGTCCGAATGGGAGAAGTACGACGAGCCGTACAAGCAGA
CGTATTCGGAGTACGTGAAGATCCAGCGTGAGAAGGATTCAGGCGCCTATTCGGTGAAGGGCGCGCTGGAGCGGAGCAAG
ATGCTGGAAAACGCTGACCCAGGCTGGATCAGCGTGATCAAGGCGCACTACGGTGCGATCGCGCGTGCGGAGTATGCGGC
AGCGTCCGCGGAGTCGCGCATGGCGCGCTTCGCCAAGGCGCCGGGTCAGCGCAACATGGCGACGATGGGCATGCTCGACG
AGATCCGCCATGGCCAGATCCAGCTGTTCTTCCCGCACGAGCACGTGTCGAAAGATCGTCAATTCGACTGGGCATTCAAG
GCCTACGACACGAATGAATGGGGCGCCATTGCCGCGCGCCACATGTTCGACGACATGATGAACACGCGCAGTGCGGTGGC
CATCGGGTTGATGTTGACCTTCGCCTTCGAGACCGGTTTCACCAACATGCAGTTCCTCGGTCTGGCAGCGGACGCCGCTG
AGGCGGGCGACTGGACCTTCGCCAGCATGATCTCCAGCGTCCAGACGGATGAGTCTCGTCATGCTCAGATCGGCGGTCCG
CTGGTGCCGATCCTGATCGCGAACGGCAAGAAGGCCGAGGCTCAACGCATGATCGACGTTGCGTTCTGGCGCTCTTGGAA
GCTGTTCACTGTGCTGACCGGACCGATGATGGACTACTACACGCCGCTCGCGCATCGCAAGCAGTCGTTCAAGGAGTTCA
TGCAGGAGTTCATCGTCACGCAGTTCGAGCGCTCCATCCTGGATCTGGGACTCGAACGACCCTGGTACTGGGACCAGTTC
CTGGCGGAACTGGATTACCAGCACCATGGCATGCACCTGGGGGTGTGGTTCTGGCGTCCAACCGTCTGGTGGAACCCGGC
TGCAGGCGTCACGCCCGAGGAACGTGCGTGGCTGGAGGAAAAGTATCCGGGCTGGAACGACACGTGGGGCAAGAGCTGGG
ACGTCATCGTCGACAACCTGTTGAAAGACAAGCGCGAGCTCACGTATCCGGAAACGCTTCCGGTGGTGTGCAACATGTGC
AATCTGCCGATCAACGCAACACCTGGCGATCCCTGGAAGGTCCGAGACCATTCGTTGGAGCGCAAGTCGCGCTGGTATCA
CTTCTGTTCAGAAGGCTGCAAATGGTGCTTCGAGCAGGAGCCTGAGCGCTACGAGGGCCACCTGTCGCTGATCGATCGCT
TCCTCGCAGGGTTGATCCAGCCGATGGACCTGGGCGGAGGTCTCAAGTACATGGGCCTGGCACCCGGCGAGATCGGTGAT
GACGCCCATGGCTATGCCTGGCTCGATGCCTACCGCCAAGTGCCCAAGGCAGCCGCCTGA

Upstream 100 bases:

>100_bases
GCCTCCGTCGATTCGATGCGGTGACTTGGATCGAACGTGTCTGTGTATCAAAGCCCGAGCGCGTTGCCGCTCGGATCTGT
CAGGTCTAACGGAGAGAACG

Downstream 100 bases:

>100_bases
GCTTCATCGCCGCAACATCAGTTCGACGACCTCACTCCACCGGCGACTTCGGTTCGCCGGGAGTAATCCCTTTTGCCTGA
AGGAGCATTCGATGGCCCTT

Product: toluene monooxygenase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MALLERMDWYDLARTTNWTPTYVSEAELFPTEMSGDMGIPMSEWEKYDEPYKQTYSEYVKIQREKDSGAYSVKGALERSK
MLENADPGWISVIKAHYGAIARAEYAAASAESRMARFAKAPGQRNMATMGMLDEIRHGQIQLFFPHEHVSKDRQFDWAFK
AYDTNEWGAIAARHMFDDMMNTRSAVAIGLMLTFAFETGFTNMQFLGLAADAAEAGDWTFASMISSVQTDESRHAQIGGP
LVPILIANGKKAEAQRMIDVAFWRSWKLFTVLTGPMMDYYTPLAHRKQSFKEFMQEFIVTQFERSILDLGLERPWYWDQF
LAELDYQHHGMHLGVWFWRPTVWWNPAAGVTPEERAWLEEKYPGWNDTWGKSWDVIVDNLLKDKRELTYPETLPVVCNMC
NLPINATPGDPWKVRDHSLERKSRWYHFCSEGCKWCFEQEPERYEGHLSLIDRFLAGLIQPMDLGGGLKYMGLAPGEIGD
DAHGYAWLDAYRQVPKAAA

Sequences:

>Translated_499_residues
MALLERMDWYDLARTTNWTPTYVSEAELFPTEMSGDMGIPMSEWEKYDEPYKQTYSEYVKIQREKDSGAYSVKGALERSK
MLENADPGWISVIKAHYGAIARAEYAAASAESRMARFAKAPGQRNMATMGMLDEIRHGQIQLFFPHEHVSKDRQFDWAFK
AYDTNEWGAIAARHMFDDMMNTRSAVAIGLMLTFAFETGFTNMQFLGLAADAAEAGDWTFASMISSVQTDESRHAQIGGP
LVPILIANGKKAEAQRMIDVAFWRSWKLFTVLTGPMMDYYTPLAHRKQSFKEFMQEFIVTQFERSILDLGLERPWYWDQF
LAELDYQHHGMHLGVWFWRPTVWWNPAAGVTPEERAWLEEKYPGWNDTWGKSWDVIVDNLLKDKRELTYPETLPVVCNMC
NLPINATPGDPWKVRDHSLERKSRWYHFCSEGCKWCFEQEPERYEGHLSLIDRFLAGLIQPMDLGGGLKYMGLAPGEIGD
DAHGYAWLDAYRQVPKAAA
>Mature_498_residues
ALLERMDWYDLARTTNWTPTYVSEAELFPTEMSGDMGIPMSEWEKYDEPYKQTYSEYVKIQREKDSGAYSVKGALERSKM
LENADPGWISVIKAHYGAIARAEYAAASAESRMARFAKAPGQRNMATMGMLDEIRHGQIQLFFPHEHVSKDRQFDWAFKA
YDTNEWGAIAARHMFDDMMNTRSAVAIGLMLTFAFETGFTNMQFLGLAADAAEAGDWTFASMISSVQTDESRHAQIGGPL
VPILIANGKKAEAQRMIDVAFWRSWKLFTVLTGPMMDYYTPLAHRKQSFKEFMQEFIVTQFERSILDLGLERPWYWDQFL
AELDYQHHGMHLGVWFWRPTVWWNPAAGVTPEERAWLEEKYPGWNDTWGKSWDVIVDNLLKDKRELTYPETLPVVCNMCN
LPINATPGDPWKVRDHSLERKSRWYHFCSEGCKWCFEQEPERYEGHLSLIDRFLAGLIQPMDLGGGLKYMGLAPGEIGDD
AHGYAWLDAYRQVPKAAA

Specific function: Hydroxylates toluene to form p-cresol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR003430
- InterPro:   IPR012348
- InterPro:   IPR007029 [H]

Pfam domain/function: PF02332 Phenol_Hydrox; PF04945 YHS [H]

EC number: NA

Molecular weight: Translated: 57480; Mature: 57348

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLERMDWYDLARTTNWTPTYVSEAELFPTEMSGDMGIPMSEWEKYDEPYKQTYSEYVK
CCHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHH
IQREKDSGAYSVKGALERSKMLENADPGWISVIKAHYGAIARAEYAAASAESRMARFAKA
HHCCCCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PGQRNMATMGMLDEIRHGQIQLFFPHEHVSKDRQFDWAFKAYDTNEWGAIAARHMFDDMM
CCCCCHHHHHHHHHHCCCEEEEEECHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
NTRSAVAIGLMLTFAFETGFTNMQFLGLAADAAEAGDWTFASMISSVQTDESRHAQIGGP
HHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHCCCC
LVPILIANGKKAEAQRMIDVAFWRSWKLFTVLTGPMMDYYTPLAHRKQSFKEFMQEFIVT
EEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHCHHHHHHHHHHHHHHHHHHH
QFERSILDLGLERPWYWDQFLAELDYQHHGMHLGVWFWRPTVWWNPAAGVTPEERAWLEE
HHHHHHHHHCCCCCCHHHHHHHHHCHHHCCEEEEEEEECCEEEECCCCCCCCHHHHHHHH
KYPGWNDTWGKSWDVIVDNLLKDKRELTYPETLPVVCNMCNLPINATPGDPWKVRDHSLE
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
RKSRWYHFCSEGCKWCFEQEPERYEGHLSLIDRFLAGLIQPMDLGGGLKYMGLAPGEIGD
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCC
DAHGYAWLDAYRQVPKAAA
CCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ALLERMDWYDLARTTNWTPTYVSEAELFPTEMSGDMGIPMSEWEKYDEPYKQTYSEYVK
CHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHH
IQREKDSGAYSVKGALERSKMLENADPGWISVIKAHYGAIARAEYAAASAESRMARFAKA
HHCCCCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PGQRNMATMGMLDEIRHGQIQLFFPHEHVSKDRQFDWAFKAYDTNEWGAIAARHMFDDMM
CCCCCHHHHHHHHHHCCCEEEEEECHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
NTRSAVAIGLMLTFAFETGFTNMQFLGLAADAAEAGDWTFASMISSVQTDESRHAQIGGP
HHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHCCCC
LVPILIANGKKAEAQRMIDVAFWRSWKLFTVLTGPMMDYYTPLAHRKQSFKEFMQEFIVT
EEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHCHHHHHHHHHHHHHHHHHHH
QFERSILDLGLERPWYWDQFLAELDYQHHGMHLGVWFWRPTVWWNPAAGVTPEERAWLEE
HHHHHHHHHCCCCCCHHHHHHHHHCHHHCCEEEEEEEECCEEEECCCCCCCCHHHHHHHH
KYPGWNDTWGKSWDVIVDNLLKDKRELTYPETLPVVCNMCNLPINATPGDPWKVRDHSLE
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
RKSRWYHFCSEGCKWCFEQEPERYEGHLSLIDRFLAGLIQPMDLGGGLKYMGLAPGEIGD
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCC
DAHGYAWLDAYRQVPKAAA
CCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1885512 [H]