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The map label for this gene is gcvA [H]

Identifier: 124265938

GI number: 124265938

Start: 773811

End: 774773

Strand: Reverse

Name: gcvA [H]

Synonym: Mpe_A0745

Alternate gene names: 124265938

Gene position: 774773-773811 (Counterclockwise)

Preceding gene: 124265942

Following gene: 124265935

Centisome position: 19.16

GC content: 70.61

Gene sequence:

>963_bases
ATGTCACTCCCTCTCGTACGACTGCCATCGCTCGACCTGATCCGGGGTTTCGTCGCGGTGGGACGCCGCATGAGCATCAC
GCTCGCGTCGCAGGACCTGTGCCTCACGCAATCCGCCGTCAGTCGGCAGATCGGCGCGCTGGAAGAGCAGCTCGGCGTGA
AGCTGCTGGTCCGCGGCTACCGCTCCATCGCGTTCACGGCCGAAGGCGAACGGCTGTTCCGCAGCGCCGACGGCGCGGTG
CAGCAGATGCAGGACGTGATGGGCGAGATCCACACCACCGGCGTGCTGCGACCGGTGATGCTGAGCGCGAGCATCGGCGT
GACGGGCCTGTGGCTGCTGCCGCGGCTGAGCCGGTTCCAGAAGCTGCATCCGGGCGTGGACCTGCGCGTGTCTGCGAACA
ACCGCGTGGCCGACCTGCGCAGCGACGGCATCGACCTGGCCATCCGCTACACCACGCCGGCCTTGGCGCCGGCCGGGGCG
ACCCGCCTGTTCGGCGAATCCGTCGCGCCGGTGGCCCACCCTTCACTGGGCCTCAAGTCCCTGCGGTCGGCGCAGGCGCT
CGCGAAGGTCTCGCTGCTGGAGTTCGACGACCCGACGCACCCCTGGCTGCGATGGGGCGACTGGCTCGGTGCCGCAGGCT
GGTCCGACGTGCGGCACCAGGGCATGCTGCACTTCAACCAGTACGACCAGGTGATCCAGGCCGCGCTGGACGCGCAGGGC
GTGGCGCTGGGGCGCATCGAGCTGATCCAGCCGCTGCTCGACGCCGGCCGGCTGGTCCGGCTGGCCCCACCGAACCACCG
TGCGGACATGGAGCACGCCTACTGGCTCATCCTCGCCAACGATCGTCCCCGCGCCGACATCCGTCGCGTGGCGGAGTGGA
TAGAAAGCGAGGCGCGAATGTCGGCCGAGCCCGCTCTCGCGCCCCCGCAGACGGCACCGAAGCCGCGCGCCCGGCGTTCC
TGA

Upstream 100 bases:

>100_bases
AAGCAGGGGCCGGCACGCATGCCGGTTCGAGGTTCGGATTACAGGACCGCGCCGCCTGTGCTTCAATCGATCATTCCGTC
GCATCGCATACGCCCCAGGA

Downstream 100 bases:

>100_bases
GTCGTCCCTACGCCGGCGCCTGGACGCAGTGGTCCAGGGCATCGTCCAGGCGATCGTTGCCCCAGAACATGTCGTCGCCC
ACGAAGAACATCGGCGCACC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSLPLVRLPSLDLIRGFVAVGRRMSITLASQDLCLTQSAVSRQIGALEEQLGVKLLVRGYRSIAFTAEGERLFRSADGAV
QQMQDVMGEIHTTGVLRPVMLSASIGVTGLWLLPRLSRFQKLHPGVDLRVSANNRVADLRSDGIDLAIRYTTPALAPAGA
TRLFGESVAPVAHPSLGLKSLRSAQALAKVSLLEFDDPTHPWLRWGDWLGAAGWSDVRHQGMLHFNQYDQVIQAALDAQG
VALGRIELIQPLLDAGRLVRLAPPNHRADMEHAYWLILANDRPRADIRRVAEWIESEARMSAEPALAPPQTAPKPRARRS

Sequences:

>Translated_320_residues
MSLPLVRLPSLDLIRGFVAVGRRMSITLASQDLCLTQSAVSRQIGALEEQLGVKLLVRGYRSIAFTAEGERLFRSADGAV
QQMQDVMGEIHTTGVLRPVMLSASIGVTGLWLLPRLSRFQKLHPGVDLRVSANNRVADLRSDGIDLAIRYTTPALAPAGA
TRLFGESVAPVAHPSLGLKSLRSAQALAKVSLLEFDDPTHPWLRWGDWLGAAGWSDVRHQGMLHFNQYDQVIQAALDAQG
VALGRIELIQPLLDAGRLVRLAPPNHRADMEHAYWLILANDRPRADIRRVAEWIESEARMSAEPALAPPQTAPKPRARRS
>Mature_319_residues
SLPLVRLPSLDLIRGFVAVGRRMSITLASQDLCLTQSAVSRQIGALEEQLGVKLLVRGYRSIAFTAEGERLFRSADGAVQ
QMQDVMGEIHTTGVLRPVMLSASIGVTGLWLLPRLSRFQKLHPGVDLRVSANNRVADLRSDGIDLAIRYTTPALAPAGAT
RLFGESVAPVAHPSLGLKSLRSAQALAKVSLLEFDDPTHPWLRWGDWLGAAGWSDVRHQGMLHFNQYDQVIQAALDAQGV
ALGRIELIQPLLDAGRLVRLAPPNHRADMEHAYWLILANDRPRADIRRVAEWIESEARMSAEPALAPPQTAPKPRARRS

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=302, Percent_Identity=32.4503311258278, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1786448, Length=249, Percent_Identity=32.1285140562249, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788706, Length=257, Percent_Identity=28.0155642023346, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI145693193, Length=270, Percent_Identity=29.2592592592593, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081978, Length=271, Percent_Identity=29.1512915129151, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1786401, Length=257, Percent_Identity=28.4046692607004, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787589, Length=256, Percent_Identity=25.390625, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35134; Mature: 35003

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPLVRLPSLDLIRGFVAVGRRMSITLASQDLCLTQSAVSRQIGALEEQLGVKLLVRGY
CCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSIAFTAEGERLFRSADGAVQQMQDVMGEIHTTGVLRPVMLSASIGVTGLWLLPRLSRFQ
HHEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KLHPGVDLRVSANNRVADLRSDGIDLAIRYTTPALAPAGATRLFGESVAPVAHPSLGLKS
HHCCCCEEEECCCCCEEHHCCCCCEEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCHHH
LRSAQALAKVSLLEFDDPTHPWLRWGDWLGAAGWSDVRHQGMLHFNQYDQVIQAALDAQG
HHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCCCHHHHHCCCEEHHHHHHHHHHHHCCCC
VALGRIELIQPLLDAGRLVRLAPPNHRADMEHAYWLILANDRPRADIRRVAEWIESEARM
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHC
SAEPALAPPQTAPKPRARRS
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLPLVRLPSLDLIRGFVAVGRRMSITLASQDLCLTQSAVSRQIGALEEQLGVKLLVRGY
CCCCCCCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSIAFTAEGERLFRSADGAVQQMQDVMGEIHTTGVLRPVMLSASIGVTGLWLLPRLSRFQ
HHEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KLHPGVDLRVSANNRVADLRSDGIDLAIRYTTPALAPAGATRLFGESVAPVAHPSLGLKS
HHCCCCEEEECCCCCEEHHCCCCCEEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCHHH
LRSAQALAKVSLLEFDDPTHPWLRWGDWLGAAGWSDVRHQGMLHFNQYDQVIQAALDAQG
HHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCCCHHHHHCCCEEHHHHHHHHHHHHCCCC
VALGRIELIQPLLDAGRLVRLAPPNHRADMEHAYWLILANDRPRADIRRVAEWIESEARM
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHC
SAEPALAPPQTAPKPRARRS
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]