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The map label for this gene is algU [H]

Identifier: 124265835

GI number: 124265835

Start: 676026

End: 676646

Strand: Direct

Name: algU [H]

Synonym: Mpe_A0642

Alternate gene names: 124265835

Gene position: 676026-676646 (Clockwise)

Preceding gene: 124265834

Following gene: 124265836

Centisome position: 16.72

GC content: 65.22

Gene sequence:

>621_bases
GTGGCTGAGCCGACCAGTCCTGACAACGACGCCCTGCTGGTCGACCGCGTCAAGCAGGGCGACGTACGCGCCTTCGAGAT
GCTGGTCGTCAAGTACCAGCGGCGGATCGAGCGCCTGATCGGGCGCATGGTGCGCGACGTCGACCTGGTCCAGGACATCG
CGCAGGAGACCTTCATCCGCGCCTACCGGGCGATCCCGCAGTTCCGTGGCGAGAGCCAGTTCTACACCTGGCTGTACCGG
ATCGCGGTCAACACGGCCAAGAAGACCCTCGGCGACCTGAAGCGAGACCTGCTCGTGACCGAATCGGCACGGGCCGGCCG
GGACGAGGACGGCGATGAAACTTCCCGGGTCGAGAACGAACTAACCGACGGCGAGACGCCTGACGCCTTGCTGGCATCGA
AACAGATCGCCAATGCGGTGAACTCCGCGATCGAGGATCTGTCCGAAGATCTGAGGCAGGCGATCACGCTGCGCGAGATC
GAAGGCCTGAGCTACGAAGAGATCGCTGACGTGATGAATTGCCCGATCGGGACGGTGCGGTCGCGCATTTTCCGGGCGCG
CGAGGCGATCGCGGAGCGTTTGCGCCCGATGCTGGAGCAGCGCTCCGGCGAGCGCTGGTGA

Upstream 100 bases:

>100_bases
GGCGTTGCGGCGCCACGAGCATCCGGCCGAATGGCATGCGCTGCGCTGTGCGCTCTATTCGCCCGCGGCATCGTCCGGCG
CATCGGAAGGTCCCTGAGCC

Downstream 100 bases:

>100_bases
GTAGTGTGGGCGAGCCCAGGGGCCAACGGAGTGTTCAGATGAGCAATGTCGAGAACCTGCAGGGGGCCGAGGCCCTTTCG
GCCCTGGTCGATGGCGAGGC

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MAEPTSPDNDALLVDRVKQGDVRAFEMLVVKYQRRIERLIGRMVRDVDLVQDIAQETFIRAYRAIPQFRGESQFYTWLYR
IAVNTAKKTLGDLKRDLLVTESARAGRDEDGDETSRVENELTDGETPDALLASKQIANAVNSAIEDLSEDLRQAITLREI
EGLSYEEIADVMNCPIGTVRSRIFRAREAIAERLRPMLEQRSGERW

Sequences:

>Translated_206_residues
MAEPTSPDNDALLVDRVKQGDVRAFEMLVVKYQRRIERLIGRMVRDVDLVQDIAQETFIRAYRAIPQFRGESQFYTWLYR
IAVNTAKKTLGDLKRDLLVTESARAGRDEDGDETSRVENELTDGETPDALLASKQIANAVNSAIEDLSEDLRQAITLREI
EGLSYEEIADVMNCPIGTVRSRIFRAREAIAERLRPMLEQRSGERW
>Mature_205_residues
AEPTSPDNDALLVDRVKQGDVRAFEMLVVKYQRRIERLIGRMVRDVDLVQDIAQETFIRAYRAIPQFRGESQFYTWLYRI
AVNTAKKTLGDLKRDLLVTESARAGRDEDGDETSRVENELTDGETPDALLASKQIANAVNSAIEDLSEDLRQAITLREIE
GLSYEEIADVMNCPIGTVRSRIFRAREAIAERLRPMLEQRSGERW

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=193, Percent_Identity=50.259067357513, Blast_Score=181, Evalue=4e-47,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 23561; Mature: 23430

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPTSPDNDALLVDRVKQGDVRAFEMLVVKYQRRIERLIGRMVRDVDLVQDIAQETFIR
CCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYRAIPQFRGESQFYTWLYRIAVNTAKKTLGDLKRDLLVTESARAGRDEDGDETSRVENE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCHHHHHHHHH
LTDGETPDALLASKQIANAVNSAIEDLSEDLRQAITLREIEGLSYEEIADVMNCPIGTVR
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHH
SRIFRAREAIAERLRPMLEQRSGERW
HHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AEPTSPDNDALLVDRVKQGDVRAFEMLVVKYQRRIERLIGRMVRDVDLVQDIAQETFIR
CCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYRAIPQFRGESQFYTWLYRIAVNTAKKTLGDLKRDLLVTESARAGRDEDGDETSRVENE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCHHHHHHHHH
LTDGETPDALLASKQIANAVNSAIEDLSEDLRQAITLREIEGLSYEEIADVMNCPIGTVR
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHH
SRIFRAREAIAERLRPMLEQRSGERW
HHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]