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The map label for this gene is gacA [H]

Identifier: 124265774

GI number: 124265774

Start: 609764

End: 610396

Strand: Reverse

Name: gacA [H]

Synonym: Mpe_A0581

Alternate gene names: 124265774

Gene position: 610396-609764 (Counterclockwise)

Preceding gene: 124265775

Following gene: 124265773

Centisome position: 15.09

GC content: 68.09

Gene sequence:

>633_bases
ATGACCGTCCGCGTCATCCTCTGCGACGACCACGCACTGATCCGGCGCGGCATCCGCGACACGCTGTCGGATGCGCCCGA
CATCGAGGTCGTGGGCGAGGCGGGCGACTACGGCGAGCTGCGCAGCCTGCTGCGCGACACCGACTGCGACGTGCTGGTGC
TCGACATCAACCTGCCGGGTCGCAGCGGCCTCGACGTGCTGCACGTGCTGAAGGAGGAAGGCTCGGCGCTGCGCGTGCTG
GTGGTGTCGATGTACCCCGAGGACCAGTATGCGATCCGCGCGCTGCGCGCCGGCGCCTACGGCTACGTCAACAAGGGCAG
CGACACCCAGCTCATCGTCGAGGCGGTGCGGATGGTGGCCAAGGGCCGCAAGTACATCACGCCCGAGATCGCGCAGATGC
TGGCCGAGAGCCTGAGCCAGCCGGCCACCGAGATCGCCCACGAGAAGCTGTCGGACCGCGAGCTGCAGACGCTGGTGCTG
ATCGCGTCGGGCAAGCGGCTGTCGGACATCGCCGCCGAACTGACGCTGAGCCCGAAGACCGTCAGCGTCTACCGCGCCCG
CGTGCTGGAGAAGCTGGCCCTGACCAACAACTCCGAGCTCACGGTCTACGCCATCCGAAACGGCCTGGTATGA

Upstream 100 bases:

>100_bases
GACATGCTGCGGTGAAGGCGGCCGCTGCGACGGGCTGGCCCGACGGGCCGTGACCGCGGTAGAGTGGAAGCATGCAGTCA
TCCTCCCTCGCAGCGCCGTC

Downstream 100 bases:

>100_bases
GGCTCGGCCGCGACCGAGCCTGACCCCCGTCTCCATTCATCTTGTGCAACGCCCCGGGAGAGCCGCGATGCTGAGCCTGC
GCCCCAACTGCGAATGCTGC

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MTVRVILCDDHALIRRGIRDTLSDAPDIEVVGEAGDYGELRSLLRDTDCDVLVLDINLPGRSGLDVLHVLKEEGSALRVL
VVSMYPEDQYAIRALRAGAYGYVNKGSDTQLIVEAVRMVAKGRKYITPEIAQMLAESLSQPATEIAHEKLSDRELQTLVL
IASGKRLSDIAAELTLSPKTVSVYRARVLEKLALTNNSELTVYAIRNGLV

Sequences:

>Translated_210_residues
MTVRVILCDDHALIRRGIRDTLSDAPDIEVVGEAGDYGELRSLLRDTDCDVLVLDINLPGRSGLDVLHVLKEEGSALRVL
VVSMYPEDQYAIRALRAGAYGYVNKGSDTQLIVEAVRMVAKGRKYITPEIAQMLAESLSQPATEIAHEKLSDRELQTLVL
IASGKRLSDIAAELTLSPKTVSVYRARVLEKLALTNNSELTVYAIRNGLV
>Mature_209_residues
TVRVILCDDHALIRRGIRDTLSDAPDIEVVGEAGDYGELRSLLRDTDCDVLVLDINLPGRSGLDVLHVLKEEGSALRVLV
VSMYPEDQYAIRALRAGAYGYVNKGSDTQLIVEAVRMVAKGRKYITPEIAQMLAESLSQPATEIAHEKLSDRELQTLVLI
ASGKRLSDIAAELTLSPKTVSVYRARVLEKLALTNNSELTVYAIRNGLV

Specific function: Positively controls the production of the autoinducer N- butyryl-homoserine lactone and the formation of the virulence factors pyocyanine, cyanide, and lipase [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=208, Percent_Identity=37.9807692307692, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1788521, Length=190, Percent_Identity=36.8421052631579, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1790102, Length=199, Percent_Identity=36.1809045226131, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1786747, Length=200, Percent_Identity=33.5, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788712, Length=207, Percent_Identity=28.5024154589372, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787473, Length=182, Percent_Identity=34.0659340659341, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788546, Length=206, Percent_Identity=30.5825242718447, Blast_Score=77, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23046; Mature: 22915

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRVILCDDHALIRRGIRDTLSDAPDIEVVGEAGDYGELRSLLRDTDCDVLVLDINLPG
CEEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCCCEEEEEEECCC
RSGLDVLHVLKEEGSALRVLVVSMYPEDQYAIRALRAGAYGYVNKGSDTQLIVEAVRMVA
CCCHHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
KGRKYITPEIAQMLAESLSQPATEIAHEKLSDRELQTLVLIASGKRLSDIAAELTLSPKT
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHCCCCCH
VSVYRARVLEKLALTNNSELTVYAIRNGLV
HHHHHHHHHHHHHHCCCCCEEEEEECCCCC
>Mature Secondary Structure 
TVRVILCDDHALIRRGIRDTLSDAPDIEVVGEAGDYGELRSLLRDTDCDVLVLDINLPG
EEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCCCEEEEEEECCC
RSGLDVLHVLKEEGSALRVLVVSMYPEDQYAIRALRAGAYGYVNKGSDTQLIVEAVRMVA
CCCHHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
KGRKYITPEIAQMLAESLSQPATEIAHEKLSDRELQTLVLIASGKRLSDIAAELTLSPKT
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHCCCCCH
VSVYRARVLEKLALTNNSELTVYAIRNGLV
HHHHHHHHHHHHHHCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9159518; 10984043 [H]