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The map label for this gene is smf [H]

Identifier: 124265474

GI number: 124265474

Start: 306357

End: 307454

Strand: Reverse

Name: smf [H]

Synonym: Mpe_A0281

Alternate gene names: 124265474

Gene position: 307454-306357 (Counterclockwise)

Preceding gene: 124265475

Following gene: 124265473

Centisome position: 7.6

GC content: 68.67

Gene sequence:

>1098_bases
ATGATCGAGCGTGACGATCTCGCAGCGTGGCTGCGCTTGCTCGAAACTCCGTCGATCGGGAGGGATACCGCGCGCCGCCT
GCTGGCGGCTTTCGGTTCCCCTCAGGCCGTCTTTGACGCTCCGCCCGTCGCCCTGAGAGAGCTGCTGACGGCGGAAAAGG
CCGCGGCGTTGCGGTCGCCGCCCCCCTCGCTCGACGTACTGATCGAAGCGACTTGGCAGTGGCTTGACGCAGGGGAGGAG
CGCCACGTGGTGGCGCTCGGCGACCCGGCCTACCCCCGCGCTTTGCTCGAGACCGCCGACCCACCCCTGCTGATCCATGC
CGTGGGCCGCTTGGCGTTGCTGAATGCACCGAGCGTGGCCGTGGTTGGCAGCCGCAATCCGACGCCGCAGGGCGCCGAGA
ACGCCCGCGCCTTCGCCACCGCCCTGAGCCACGCCGGCCTGACCGTTGTATCCGGGCTGGCGCTCGGCATCGATGGCGCC
GCCCACGACGGTGCACTGGCAGGCGAGGGGTCGACGATCGCGGTCGTCGGCACCGGGCTCGATCGCGTCTATCCGAAGCG
GCATCTGAAGTTGGCTCACCGGATTGCCCGCGATGGCCTGATGGTGAGCGAGTACGCGCCGGGAACGCCCCCGATTGCCG
CACACTTCCCACTGCGCAACCGCCTGATCGCCGGCCTGACCCGAGGTACGCTGGTGGTCGAGGCCGCGTTGCAGTCGGGC
TCGCTGATCACCGCACGGCTGGCGCTCGAAGCCGGTCGGGAGGTCTTCGCGATTCCGGGCTCGATTCACGCGCCCCAGTC
ACGCGGCTGTCACGCGCTGATCAAGCAGGGCGCCAAACTGGTCGACAGCGCGGCGGACATCCTTGAGGAACTGCGGTGGT
TCGACGCGCCAGACAGGCCCTCGCCCACCACGTCAAGCCCGTCCGTAGAAGACCCGGTACTGGCCGCCCTCGGCCACGAT
CCCGTCACTCTTGACGCACTGAGCGCCCGGATCGGGTGGCCCCCAGCCGAATTGAGCGCTCGCTTACTGGCACTTGAACT
GAGTGGCGACGTGGTTCGTTTGCCAGGCCAGTTGTTCCAGCGCCTCGTACAGGCCTGA

Upstream 100 bases:

>100_bases
CCTGAACGTCAACGACCCGGTCACCCGCGGGGATCGCTTTACCGAGCCCTGAGTTCGCCCTCCGGGTCCCGGCGCTCACT
GAGCCATTGCGCCGCCGGCC

Downstream 100 bases:

>100_bases
GACCCTGCACGCTGAACGCCCCGCATCCGTGCCGGCGAATGCCGGCGGCGCCCTTCGCCTGACACCCCCTGTGGTATCTT
GTCGCATGTTCGACGTGCTC

Product: putative SMF protein

Products: NA

Alternate protein names: DNA-processing chain A [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MIERDDLAAWLRLLETPSIGRDTARRLLAAFGSPQAVFDAPPVALRELLTAEKAAALRSPPPSLDVLIEATWQWLDAGEE
RHVVALGDPAYPRALLETADPPLLIHAVGRLALLNAPSVAVVGSRNPTPQGAENARAFATALSHAGLTVVSGLALGIDGA
AHDGALAGEGSTIAVVGTGLDRVYPKRHLKLAHRIARDGLMVSEYAPGTPPIAAHFPLRNRLIAGLTRGTLVVEAALQSG
SLITARLALEAGREVFAIPGSIHAPQSRGCHALIKQGAKLVDSAADILEELRWFDAPDRPSPTTSSPSVEDPVLAALGHD
PVTLDALSARIGWPPAELSARLLALELSGDVVRLPGQLFQRLVQA

Sequences:

>Translated_365_residues
MIERDDLAAWLRLLETPSIGRDTARRLLAAFGSPQAVFDAPPVALRELLTAEKAAALRSPPPSLDVLIEATWQWLDAGEE
RHVVALGDPAYPRALLETADPPLLIHAVGRLALLNAPSVAVVGSRNPTPQGAENARAFATALSHAGLTVVSGLALGIDGA
AHDGALAGEGSTIAVVGTGLDRVYPKRHLKLAHRIARDGLMVSEYAPGTPPIAAHFPLRNRLIAGLTRGTLVVEAALQSG
SLITARLALEAGREVFAIPGSIHAPQSRGCHALIKQGAKLVDSAADILEELRWFDAPDRPSPTTSSPSVEDPVLAALGHD
PVTLDALSARIGWPPAELSARLLALELSGDVVRLPGQLFQRLVQA
>Mature_365_residues
MIERDDLAAWLRLLETPSIGRDTARRLLAAFGSPQAVFDAPPVALRELLTAEKAAALRSPPPSLDVLIEATWQWLDAGEE
RHVVALGDPAYPRALLETADPPLLIHAVGRLALLNAPSVAVVGSRNPTPQGAENARAFATALSHAGLTVVSGLALGIDGA
AHDGALAGEGSTIAVVGTGLDRVYPKRHLKLAHRIARDGLMVSEYAPGTPPIAAHFPLRNRLIAGLTRGTLVVEAALQSG
SLITARLALEAGREVFAIPGSIHAPQSRGCHALIKQGAKLVDSAADILEELRWFDAPDRPSPTTSSPSVEDPVLAALGHD
PVTLDALSARIGWPPAELSARLLALELSGDVVRLPGQLFQRLVQA

Specific function: Essential for efficient chromosomal DNA transformation but not required for plasmid transformation [H]

COG id: COG0758

COG function: function code LU; Predicted Rossmann fold nucleotide-binding protein involved in DNA uptake

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smf family [H]

Homologues:

Organism=Escherichia coli, GI48994932, Length=363, Percent_Identity=41.5977961432507, Blast_Score=222, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003488 [H]

Pfam domain/function: PF02481 DNA_processg_A [H]

EC number: NA

Molecular weight: Translated: 38338; Mature: 38338

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERDDLAAWLRLLETPSIGRDTARRLLAAFGSPQAVFDAPPVALRELLTAEKAAALRSP
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
PPSLDVLIEATWQWLDAGEERHVVALGDPAYPRALLETADPPLLIHAVGRLALLNAPSVA
CCCCEEHEEHHHHHHCCCCCCEEEEECCCCCHHHHHHCCCCCEEHHHHHHHHEECCCCEE
VVGSRNPTPQGAENARAFATALSHAGLTVVSGLALGIDGAAHDGALAGEGSTIAVVGTGL
EECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCH
DRVYPKRHLKLAHRIARDGLMVSEYAPGTPPIAAHFPLRNRLIAGLTRGTLVVEAALQSG
HHHCHHHHHHHHHHHHHCCEEEECCCCCCCCEEEECCHHHHHHHHHCCCHHHEEHHHCCC
SLITARLALEAGREVFAIPGSIHAPQSRGCHALIKQGAKLVDSAADILEELRWFDAPDRP
CEEEEHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SPTTSSPSVEDPVLAALGHDPVTLDALSARIGWPPAELSARLLALELSGDVVRLPGQLFQ
CCCCCCCCCCCCHHHHCCCCCEEHHHHHHHCCCCHHHHCCEEEEEEECCCEEECCHHHHH
RLVQA
HHHCC
>Mature Secondary Structure
MIERDDLAAWLRLLETPSIGRDTARRLLAAFGSPQAVFDAPPVALRELLTAEKAAALRSP
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
PPSLDVLIEATWQWLDAGEERHVVALGDPAYPRALLETADPPLLIHAVGRLALLNAPSVA
CCCCEEHEEHHHHHHCCCCCCEEEEECCCCCHHHHHHCCCCCEEHHHHHHHHEECCCCEE
VVGSRNPTPQGAENARAFATALSHAGLTVVSGLALGIDGAAHDGALAGEGSTIAVVGTGL
EECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCH
DRVYPKRHLKLAHRIARDGLMVSEYAPGTPPIAAHFPLRNRLIAGLTRGTLVVEAALQSG
HHHCHHHHHHHHHHHHHCCEEEECCCCCCCCEEEECCHHHHHHHHHCCCHHHEEHHHCCC
SLITARLALEAGREVFAIPGSIHAPQSRGCHALIKQGAKLVDSAADILEELRWFDAPDRP
CEEEEHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SPTTSSPSVEDPVLAALGHDPVTLDALSARIGWPPAELSARLLALELSGDVVRLPGQLFQ
CCCCCCCCCCCCHHHHCCCCCEEHHHHHHHCCCCHHHHCCEEEEEEECCCEEECCHHHHH
RLVQA
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7768823; 7542800 [H]