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The map label for this gene is ptsN [H]

Identifier: 124265418

GI number: 124265418

Start: 242408

End: 242878

Strand: Reverse

Name: ptsN [H]

Synonym: Mpe_A0225

Alternate gene names: 124265418

Gene position: 242878-242408 (Counterclockwise)

Preceding gene: 124265419

Following gene: 124265417

Centisome position: 6.01

GC content: 66.03

Gene sequence:

>471_bases
ATGAACCGTCTCTCCGCCATCCTGCCCGCCAGCAACGTGCTGGTGGATGTGGACGCCACCAGCAAGAAGCGCGTGTTCGA
GCAGGCGGGCCTGCTGTTCGAGAACCGGCACGCGATCGCACGGGCGACGGTCACCGACAACCTGTTCGCCCGTGAACGCC
TGGGATCCACCGGCCTGGGACATGGCGTGGCCATCCCGCACGGCCGGATCAAGGGGCTGAAGAACCCGCTCGCGGCGGTG
ATCCGCATGCAGCAGCCGATCGGTTTCGACGCTCCGGACGACGAACCTGTGTCGCTGCTGATCTTCCTGCTGGTGCCCGA
AGCGGCCACACAGCGACACCTCGAGATCCTCTCGGAGATTGCCGAACTGCTGTCGGACCGGGCCCTGCGCGACAAGCTCA
AGGCCGAGCCCGACGCCGCCAAGCTCCACGAGTTGATTGCCAACTGGGAACCGCTGAAGTCGGTCGCGTGA

Upstream 100 bases:

>100_bases
CGCCCGAGACTCGGGATCGGCCTCCTTCCTATAATTTGTCACTCGTTTGTTGCGAAGCGCTGGCCAAGGCCCCTCCCGTC
GAAACCGGCGCTGCCCCTAG

Downstream 100 bases:

>100_bases
GCGACCTGCCGGTCCCGGCTCGAACGGTGCAGCTGGCTTGCATCGGGGGCGATGCCATGAAGCAGCCCCAACCCGCTCGT
CTTGCGACACCGAATCCGCG

Product: PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugarphosphate [C]

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MNRLSAILPASNVLVDVDATSKKRVFEQAGLLFENRHAIARATVTDNLFARERLGSTGLGHGVAIPHGRIKGLKNPLAAV
IRMQQPIGFDAPDDEPVSLLIFLLVPEAATQRHLEILSEIAELLSDRALRDKLKAEPDAAKLHELIANWEPLKSVA

Sequences:

>Translated_156_residues
MNRLSAILPASNVLVDVDATSKKRVFEQAGLLFENRHAIARATVTDNLFARERLGSTGLGHGVAIPHGRIKGLKNPLAAV
IRMQQPIGFDAPDDEPVSLLIFLLVPEAATQRHLEILSEIAELLSDRALRDKLKAEPDAAKLHELIANWEPLKSVA
>Mature_156_residues
MNRLSAILPASNVLVDVDATSKKRVFEQAGLLFENRHAIARATVTDNLFARERLGSTGLGHGVAIPHGRIKGLKNPLAAV
IRMQQPIGFDAPDDEPVSLLIFLLVPEAATQRHLEILSEIAELLSDRALRDKLKAEPDAAKLHELIANWEPLKSVA

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=151, Percent_Identity=35.7615894039735, Blast_Score=95, Evalue=2e-21,
Organism=Escherichia coli, GI1788726, Length=141, Percent_Identity=27.6595744680851, Blast_Score=62, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 17036; Mature: 17036

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRLSAILPASNVLVDVDATSKKRVFEQAGLLFENRHAIARATVTDNLFARERLGSTGLG
CCCCHHHCCCCCEEEECCCCHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCC
HGVAIPHGRIKGLKNPLAAVIRMQQPIGFDAPDDEPVSLLIFLLVPEAATQRHLEILSEI
CCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
AELLSDRALRDKLKAEPDAAKLHELIANWEPLKSVA
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure
MNRLSAILPASNVLVDVDATSKKRVFEQAGLLFENRHAIARATVTDNLFARERLGSTGLG
CCCCHHHCCCCCEEEECCCCHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCC
HGVAIPHGRIKGLKNPLAAVIRMQQPIGFDAPDDEPVSLLIFLLVPEAATQRHLEILSEI
CCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
AELLSDRALRDKLKAEPDAAKLHELIANWEPLKSVA
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein Npi-phosphohistidine; sugar [C]

Specific reaction: Protein Npi-phosphohistidine + sugar = protein histidine + sugarphosphate [C]

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]