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The map label for this gene is dmlR [H]

Identifier: 124265350

GI number: 124265350

Start: 165118

End: 166032

Strand: Reverse

Name: dmlR [H]

Synonym: Mpe_A0157

Alternate gene names: 124265350

Gene position: 166032-165118 (Counterclockwise)

Preceding gene: 124265360

Following gene: 124265349

Centisome position: 4.11

GC content: 72.13

Gene sequence:

>915_bases
ATGGATCGCCTCAAGCAGATCGAATCCTTCGCCGCCGTCGCCACCAAGGGCAGCCTGACCGCCGCCGCTGGCGCCGAGGG
CGTGGCTCCGGCCGTGATCGGCCGCCGCATCGATGCACTGGAAGAGCGCCTGGGGGTGAAGCTCCTGGTGCGCACCACCC
GGCGCATCACGCTGACGCACGAAGGCAGCGCCTTCCTCGAGGACGCCCAGCGCCTGCTGACCGATCTGGCCAATGCCGAG
GCCAGCGTCAGCGCCGGCGGCGTGAAGGCCAGTGGCCACCTGCGCCTCACCGCACCAGCCGGCTTCGGCCGGCGCCACGT
GGCGCCGCTGGTGCCGCGCTTCGCCGAGGCGCACCGCGACGTGTCGGTGTCGCTGAACCTCAGCGACCGGGTGGTCGACA
TCGTCAACGAGGGCTACGACTGCGCGGTGCGGGTCGGCGACCTGCCCGACTCCAGCCTGGTCAGCCTGCGGCTGGCCGAC
AACCGCCGCCTGTGCGTGGCGGCCCCGGCCTACCTGCAGCGCGCCGGCACGCCGCAGCATCCCGGCGAACTGGGCCGCCA
CCAGTGCCTCACGCTGAGTTCGAACGCCAGCCAGGCGCGCGGCTGGGCGTTCAGCGTCGACGGCGAACTGACGCACCTGC
GGCCGAGCGGCCGCTACGACTGCTCCGACGGCCAGGTGCTGCACGACTGGTGCCTCGCCGGCCACGGCATCGCCTGGCGC
AGCACCTGGGAGGTGGAGCAGGAGGTGGCCAGCGGCGCGCTGGTCACGCTGCTCGACGACTTCGCGGCGCCGCCGAACGG
CATCTACGCGGTGTTCCCGCAGCGCAAGCACCTGCCGCTGCGCGTGCGGCTGTGGATCGATTTCCTGAAGCACACCTACG
GCGATTCGACCTACTGGTCGCCCGCGATGCCCTGA

Upstream 100 bases:

>100_bases
ACTTTATTACGAACCTGAGTGCGCATTTAGCGACCGATTTGCGCTTGATCTTTTACTGAAGCATCAGTAATCTGCCCCGA
ATCCGCCCCATTTGCCCGCC

Downstream 100 bases:

>100_bases
ATCGCACCGGGGGCGATCTTCCCGGCCCGCCGCGGGCAATCTGCTCTGCGGCGACCGGCCCCTGGCTCCTAAGGTGACGT
CGCCCGGCATCGCGCCGGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MDRLKQIESFAAVATKGSLTAAAGAEGVAPAVIGRRIDALEERLGVKLLVRTTRRITLTHEGSAFLEDAQRLLTDLANAE
ASVSAGGVKASGHLRLTAPAGFGRRHVAPLVPRFAEAHRDVSVSLNLSDRVVDIVNEGYDCAVRVGDLPDSSLVSLRLAD
NRRLCVAAPAYLQRAGTPQHPGELGRHQCLTLSSNASQARGWAFSVDGELTHLRPSGRYDCSDGQVLHDWCLAGHGIAWR
STWEVEQEVASGALVTLLDDFAAPPNGIYAVFPQRKHLPLRVRLWIDFLKHTYGDSTYWSPAMP

Sequences:

>Translated_304_residues
MDRLKQIESFAAVATKGSLTAAAGAEGVAPAVIGRRIDALEERLGVKLLVRTTRRITLTHEGSAFLEDAQRLLTDLANAE
ASVSAGGVKASGHLRLTAPAGFGRRHVAPLVPRFAEAHRDVSVSLNLSDRVVDIVNEGYDCAVRVGDLPDSSLVSLRLAD
NRRLCVAAPAYLQRAGTPQHPGELGRHQCLTLSSNASQARGWAFSVDGELTHLRPSGRYDCSDGQVLHDWCLAGHGIAWR
STWEVEQEVASGALVTLLDDFAAPPNGIYAVFPQRKHLPLRVRLWIDFLKHTYGDSTYWSPAMP
>Mature_304_residues
MDRLKQIESFAAVATKGSLTAAAGAEGVAPAVIGRRIDALEERLGVKLLVRTTRRITLTHEGSAFLEDAQRLLTDLANAE
ASVSAGGVKASGHLRLTAPAGFGRRHVAPLVPRFAEAHRDVSVSLNLSDRVVDIVNEGYDCAVRVGDLPDSSLVSLRLAD
NRRLCVAAPAYLQRAGTPQHPGELGRHQCLTLSSNASQARGWAFSVDGELTHLRPSGRYDCSDGQVLHDWCLAGHGIAWR
STWEVEQEVASGALVTLLDDFAAPPNGIYAVFPQRKHLPLRVRLWIDFLKHTYGDSTYWSPAMP

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=292, Percent_Identity=34.5890410958904, Blast_Score=177, Evalue=6e-46,
Organism=Escherichia coli, GI1789440, Length=286, Percent_Identity=31.8181818181818, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=32.1799307958478, Blast_Score=154, Evalue=9e-39,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=31.740614334471, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=29.3103448275862, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1787128, Length=288, Percent_Identity=27.0833333333333, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1787589, Length=292, Percent_Identity=27.7397260273973, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1786448, Length=279, Percent_Identity=27.2401433691756, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1790011, Length=300, Percent_Identity=24.3333333333333, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788706, Length=282, Percent_Identity=25.177304964539, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789173, Length=268, Percent_Identity=25.3731343283582, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787601, Length=146, Percent_Identity=26.027397260274, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32942; Mature: 32942

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRLKQIESFAAVATKGSLTAAAGAEGVAPAVIGRRIDALEERLGVKLLVRTTRRITLTH
CCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCEEEEEECCEEEEEEE
EGSAFLEDAQRLLTDLANAEASVSAGGVKASGHLRLTAPAGFGRRHVAPLVPRFAEAHRD
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEEECCCCCCCCCHHHHHHHHHHHCCC
VSVSLNLSDRVVDIVNEGYDCAVRVGDLPDSSLVSLRLADNRRLCVAAPAYLQRAGTPQH
EEEEECCCHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCEEEEECHHHHHHCCCCCC
PGELGRHQCLTLSSNASQARGWAFSVDGELTHLRPSGRYDCSDGQVLHDWCLAGHGIAWR
CHHHCCCEEEEEECCCCCCCCEEEEECCCEEEECCCCCCCCCCCCCHHHHHHHCCCEEEC
STWEVEQEVASGALVTLLDDFAAPPNGIYAVFPQRKHLPLRVRLWIDFLKHTYGDSTYWS
CHHHHHHHHHCCHHEEHHHHHCCCCCCEEEECCCCCCCCEEHHHHHHHHHHHCCCCCCCC
PAMP
CCCC
>Mature Secondary Structure
MDRLKQIESFAAVATKGSLTAAAGAEGVAPAVIGRRIDALEERLGVKLLVRTTRRITLTH
CCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCEEEEEECCEEEEEEE
EGSAFLEDAQRLLTDLANAEASVSAGGVKASGHLRLTAPAGFGRRHVAPLVPRFAEAHRD
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEEECCCCCCCCCHHHHHHHHHHHCCC
VSVSLNLSDRVVDIVNEGYDCAVRVGDLPDSSLVSLRLADNRRLCVAAPAYLQRAGTPQH
EEEEECCCHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCEEEEECHHHHHHCCCCCC
PGELGRHQCLTLSSNASQARGWAFSVDGELTHLRPSGRYDCSDGQVLHDWCLAGHGIAWR
CHHHCCCEEEEEECCCCCCCCEEEEECCCEEEECCCCCCCCCCCCCHHHHHHHCCCEEEC
STWEVEQEVASGALVTLLDDFAAPPNGIYAVFPQRKHLPLRVRLWIDFLKHTYGDSTYWS
CHHHHHHHHHCCHHEEHHHHHCCCCCCEEEECCCCCCCCEEHHHHHHHHHHHCCCCCCCC
PAMP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]