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The map label for this gene is dsbA [H]

Identifier: 124265344

GI number: 124265344

Start: 158768

End: 159412

Strand: Reverse

Name: dsbA [H]

Synonym: Mpe_A0151

Alternate gene names: 124265344

Gene position: 159412-158768 (Counterclockwise)

Preceding gene: 124265345

Following gene: 124265343

Centisome position: 3.94

GC content: 66.51

Gene sequence:

>645_bases
ATGAACCGTCGTGACTTCACGACCCTGTCGCTGGCCACCACCTGCGTCGGACTGCTGCCGGCCTTCGGCCACGCCCAGGG
CGGTCCGGTCGAGGGCAAGGACTACGTGAAGCTGTCGCAGCGCCAGCCGACGCAGGACCCGAAGAAGCTCGAGGTCGTGG
AGTTCTTCTGGTACGGCTGCCCGCACTGCCACGCCTTCGAGCCGATGCTCGAAACCTGGGTCAAGAAGCTGCCGCCGGAC
ATCACCTTCCGGCGCCTGCCGGTCGCCTTCCGCGAGGTGCCGTTCGTGCTGCACCAGAAGCTGTACTTCGCGATCGAGGC
CCTGGGGCTGGTCGACACCCTGCACCGCAAGGTCTTCACCGCGATGCACGTCGAACGCAATCCGCTGAACACGCCCGAGG
CGATCGGCGACTTCGTGGCGAAGAATGGCGTCGACAAGGCCAAGTTCCTCGACGTGATGAACTCGTTCTCGGTGCAGACC
AAGGCCCGCCAGGCCGCCGCGCTGTCGGCCGGCTACAAGATCGACGGCACGCCGGCGATCGGCATCAACGGGTCCTACTA
CACCTCGGGTTCGCTGGCCGGCAGCAACGACCGCGCGCTGGCGATCACCGACCACCTGATCGCGCAGATCCGCAAGGGCG
GCTGA

Upstream 100 bases:

>100_bases
GCGCCGGCATGGTGTCGGCGCTGGTGCGGGTGCAGAAATGAACTTCGCGGCGTGGGGACGACTCCACCGCTGCGTCTGTC
TGAACGCTGAAAGGATGCTC

Downstream 100 bases:

>100_bases
CCCTCGTTTGGCGTCGACGCGACAGCGGCCGCCGGAGCGGCGGCCGGCCGTGGCAAGCGGTCGAACTCACCCTAGAATCG
GCAGCAAGATTCATGCCGAT

Product: thiol:disulfide interchange protein

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MNRRDFTTLSLATTCVGLLPAFGHAQGGPVEGKDYVKLSQRQPTQDPKKLEVVEFFWYGCPHCHAFEPMLETWVKKLPPD
ITFRRLPVAFREVPFVLHQKLYFAIEALGLVDTLHRKVFTAMHVERNPLNTPEAIGDFVAKNGVDKAKFLDVMNSFSVQT
KARQAAALSAGYKIDGTPAIGINGSYYTSGSLAGSNDRALAITDHLIAQIRKGG

Sequences:

>Translated_214_residues
MNRRDFTTLSLATTCVGLLPAFGHAQGGPVEGKDYVKLSQRQPTQDPKKLEVVEFFWYGCPHCHAFEPMLETWVKKLPPD
ITFRRLPVAFREVPFVLHQKLYFAIEALGLVDTLHRKVFTAMHVERNPLNTPEAIGDFVAKNGVDKAKFLDVMNSFSVQT
KARQAAALSAGYKIDGTPAIGINGSYYTSGSLAGSNDRALAITDHLIAQIRKGG
>Mature_214_residues
MNRRDFTTLSLATTCVGLLPAFGHAQGGPVEGKDYVKLSQRQPTQDPKKLEVVEFFWYGCPHCHAFEPMLETWVKKLPPD
ITFRRLPVAFREVPFVLHQKLYFAIEALGLVDTLHRKVFTAMHVERNPLNTPEAIGDFVAKNGVDKAKFLDVMNSFSVQT
KARQAAALSAGYKIDGTPAIGINGSYYTSGSLAGSNDRALAITDHLIAQIRKGG

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins. Involved in the production of protease and alkaline phosphatase, motility, metal resistance, and multi-drug resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioredoxin family. DsbA subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 23633; Mature: 23633

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRDFTTLSLATTCVGLLPAFGHAQGGPVEGKDYVKLSQRQPTQDPKKLEVVEFFWYGC
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEECCCCCCCCCHHHHHHHHHHCCC
PHCHAFEPMLETWVKKLPPDITFRRLPVAFREVPFVLHQKLYFAIEALGLVDTLHRKVFT
CCCCHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMHVERNPLNTPEAIGDFVAKNGVDKAKFLDVMNSFSVQTKARQAAALSAGYKIDGTPAI
HHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEE
GINGSYYTSGSLAGSNDRALAITDHLIAQIRKGG
CCCCCEEECCCCCCCCCCEEEHHHHHHHHHHCCC
>Mature Secondary Structure
MNRRDFTTLSLATTCVGLLPAFGHAQGGPVEGKDYVKLSQRQPTQDPKKLEVVEFFWYGC
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEECCCCCCCCCHHHHHHHHHHCCC
PHCHAFEPMLETWVKKLPPDITFRRLPVAFREVPFVLHQKLYFAIEALGLVDTLHRKVFT
CCCCHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMHVERNPLNTPEAIGDFVAKNGVDKAKFLDVMNSFSVQTKARQAAALSAGYKIDGTPAI
HHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEE
GINGSYYTSGSLAGSNDRALAITDHLIAQIRKGG
CCCCCEEECCCCCCCCCCEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10711598 [H]