Click here to switch to the map view.

The map label for this gene is kefB [H]

Identifier: 124265305

GI number: 124265305

Start: 114919

End: 116739

Strand: Direct

Name: kefB [H]

Synonym: Mpe_A0112

Alternate gene names: 124265305

Gene position: 114919-116739 (Clockwise)

Preceding gene: 124265304

Following gene: 124265306

Centisome position: 2.84

GC content: 71.44

Gene sequence:

>1821_bases
ATGGCGGTCGAGGCAGCAGGCAACGAACAACTGGTCCGTGTGGTGGTGCTGCTGGCCGCCGGCGTGGTGGCGGTGCCGCT
GTTCAAGCGCCTCGGCCTCGGCTCGGTGCTCGGCTACCTGGCCGCCGGGCTGGCGATCGGGCCGTTCGGCCTGGGGTTGT
TCGTCGACGCGCAGACCATCCTGCACGTGGCCGAGCTGGGCGTCGTGATGTTCCTGTTCGTCATCGGGCTGGAGATGCAG
CCCAAGCGCCTGTGGAGCCTGCGCCGCGAGATCTTCGGCCTGGGCCTGGCCCAGGTGGTGGCGTGCGGCGCATTGCTCAC
CGGCGTCGGCATGCTCGCCGGGCTGTCGGCGCCGGTGGCCTTCGTCGCCGCGATGGGCTTCGTGATGACCTCCACCGCCA
CCGTCATGCTGATCCTCGACGAGCGCGGCGAGACGGCCACCTCGCGCGGCCAGCGGGTGGTCTCGATCCTGCTGCTGGAA
GACCTGGCCATCGTGCCGCTGCTGGCCATCGTGGCCTTCCTCGCGCCGGCGCACGCCGATGTCGACAGTTCCATCTGGCG
CGCGCTGCTGCTGGCGCTGGTGGCGGTGGGCAGCGTCACGATCGCGGGGCGCTGGCTGCTCAATCCGATGTTCCGGCTGC
TGGCGGCGGCGCGCGCGCGGGAGGTGATGACCGCGGCCGCGCTGCTGGTGGTGCTGGGCGCTGCGCTGCTGATGCAGTTC
GGCGGCCTGTCGATGGCGATGGGCGCCTTCCTCGCCGGCGTGATGCTGTCGGAATCGAGCTTCCGGCATCAATTGGAGGC
GGACATCGAGCCCTTCCGCGGCATCCTGCTCGGCCTGTTCTTCCTGAGTGTCGGCATGTCGCTCGACCTCACGGTGTTCG
CCAGCCACTGGCCCCAGGTGCTGGGCGGCGTGGTGGTGTTCATGCTGGTGAAGGCGGCCGGCATCTACGTCGTGGCGCGC
GCGCTCGGCGCGCCGCACGGCGACGCGCTGCACCGCATGGTGCTGATGGCCCAGGGCGGCGAGTTCGCGTTCGTGCTGTA
CTCGGCCGGCGTCAGCGCCGGGCTGCTCGACGGCCCGATGAATGCGGTGCTCACCGCGATCGTGATCCTGTCGATGGCGC
TCACGCCGCTGGCGGTCGGGGCCCTGCGCTGGATGCTGCCGCGTGGCGCGGCCTCGCTCGAGGGCGTGGACCGGGCCGAC
GGCCTGACCGGCAGCGTGCTGATGATCGGCTTCGGCCGCTTCGGTCAGGTGGCCAGCCAGGCCCTGCTGGCGCGCGGGGT
CGACGTGGCGATCATCGACTCGGACACCGAGATGATCCAGAGCGCCGACGGCTTCGGCTTCAAGGTCTACTACGGCGACG
GCACCCGGCTCGACGTGCTGCACGCCTCCGGCGCCGCCACGGCCGAGGCGATCGCCGTCTGCGTGGACGACAAGGCGGCG
GCGCTGAAGATCGTCGAGCTGGTGAAGCACTCGTTCCCGCAGGCCCGCCTGCTGGTGCGCTCCTTCGATCGCGAGCACGC
GCTGAAGCTCATCGCCGCGGGCGTGGACTACCAGGTGCGCGAGACCTTCGAGTCGGCCAGTGCCTTCGGCGAGGCCGCGT
TGCGGGCCCTGGGCGCCACGCCCGACGAGGCCGCGCAGGTGGCGGCCGAGGTGCGCCGCCGCGACGACGAGCGCCTGCAG
CTGCAGATCACCGGCGGCCTGCGCGCGGGGGTGGGCATGATGCTCAGCAACCGCATGAAGCCCACGCCGCTGACCAAGCC
GGCCCGTGCCGGCCTCGCCTTGAACGAAGAGACCGCGTCGGTCACGGCCAACGCGCCTTAG

Upstream 100 bases:

>100_bases
CGGCGCACCGTCCCGCAGGGCCTGCAACCCGAGGTTGCCGGCCCTTTTTCAATGCGGCCCGGGGCCGCGCGTTCCCCACA
TTCGGCGAAGGAGTACAGGC

Downstream 100 bases:

>100_bases
GACCTGCGCGACACGGGCGCGAGCCCTCCGGCAGCGTCAACAGGTCCTGACCGACGCCGAGCGGCCCCGGCCCCGGCGCG
CCTTATGCGCCGCGCCGCTA

Product: Kef-type K+ transport

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 606; Mature: 605

Protein sequence:

>606_residues
MAVEAAGNEQLVRVVVLLAAGVVAVPLFKRLGLGSVLGYLAAGLAIGPFGLGLFVDAQTILHVAELGVVMFLFVIGLEMQ
PKRLWSLRREIFGLGLAQVVACGALLTGVGMLAGLSAPVAFVAAMGFVMTSTATVMLILDERGETATSRGQRVVSILLLE
DLAIVPLLAIVAFLAPAHADVDSSIWRALLLALVAVGSVTIAGRWLLNPMFRLLAAARAREVMTAAALLVVLGAALLMQF
GGLSMAMGAFLAGVMLSESSFRHQLEADIEPFRGILLGLFFLSVGMSLDLTVFASHWPQVLGGVVVFMLVKAAGIYVVAR
ALGAPHGDALHRMVLMAQGGEFAFVLYSAGVSAGLLDGPMNAVLTAIVILSMALTPLAVGALRWMLPRGAASLEGVDRAD
GLTGSVLMIGFGRFGQVASQALLARGVDVAIIDSDTEMIQSADGFGFKVYYGDGTRLDVLHASGAATAEAIAVCVDDKAA
ALKIVELVKHSFPQARLLVRSFDREHALKLIAAGVDYQVRETFESASAFGEAALRALGATPDEAAQVAAEVRRRDDERLQ
LQITGGLRAGVGMMLSNRMKPTPLTKPARAGLALNEETASVTANAP

Sequences:

>Translated_606_residues
MAVEAAGNEQLVRVVVLLAAGVVAVPLFKRLGLGSVLGYLAAGLAIGPFGLGLFVDAQTILHVAELGVVMFLFVIGLEMQ
PKRLWSLRREIFGLGLAQVVACGALLTGVGMLAGLSAPVAFVAAMGFVMTSTATVMLILDERGETATSRGQRVVSILLLE
DLAIVPLLAIVAFLAPAHADVDSSIWRALLLALVAVGSVTIAGRWLLNPMFRLLAAARAREVMTAAALLVVLGAALLMQF
GGLSMAMGAFLAGVMLSESSFRHQLEADIEPFRGILLGLFFLSVGMSLDLTVFASHWPQVLGGVVVFMLVKAAGIYVVAR
ALGAPHGDALHRMVLMAQGGEFAFVLYSAGVSAGLLDGPMNAVLTAIVILSMALTPLAVGALRWMLPRGAASLEGVDRAD
GLTGSVLMIGFGRFGQVASQALLARGVDVAIIDSDTEMIQSADGFGFKVYYGDGTRLDVLHASGAATAEAIAVCVDDKAA
ALKIVELVKHSFPQARLLVRSFDREHALKLIAAGVDYQVRETFESASAFGEAALRALGATPDEAAQVAAEVRRRDDERLQ
LQITGGLRAGVGMMLSNRMKPTPLTKPARAGLALNEETASVTANAP
>Mature_605_residues
AVEAAGNEQLVRVVVLLAAGVVAVPLFKRLGLGSVLGYLAAGLAIGPFGLGLFVDAQTILHVAELGVVMFLFVIGLEMQP
KRLWSLRREIFGLGLAQVVACGALLTGVGMLAGLSAPVAFVAAMGFVMTSTATVMLILDERGETATSRGQRVVSILLLED
LAIVPLLAIVAFLAPAHADVDSSIWRALLLALVAVGSVTIAGRWLLNPMFRLLAAARAREVMTAAALLVVLGAALLMQFG
GLSMAMGAFLAGVMLSESSFRHQLEADIEPFRGILLGLFFLSVGMSLDLTVFASHWPQVLGGVVVFMLVKAAGIYVVARA
LGAPHGDALHRMVLMAQGGEFAFVLYSAGVSAGLLDGPMNAVLTAIVILSMALTPLAVGALRWMLPRGAASLEGVDRADG
LTGSVLMIGFGRFGQVASQALLARGVDVAIIDSDTEMIQSADGFGFKVYYGDGTRLDVLHASGAATAEAIAVCVDDKAAA
LKIVELVKHSFPQARLLVRSFDREHALKLIAAGVDYQVRETFESASAFGEAALRALGATPDEAAQVAAEVRRRDDERLQL
QITGGLRAGVGMMLSNRMKPTPLTKPARAGLALNEETASVTANAP

Specific function: Transport system that facilitate potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=374, Percent_Identity=23.7967914438503, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1786232, Length=550, Percent_Identity=41.6363636363636, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1789749, Length=555, Percent_Identity=43.0630630630631, Blast_Score=390, Evalue=1e-110,
Organism=Escherichia coli, GI1786685, Length=503, Percent_Identity=26.6401590457256, Blast_Score=131, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 63317; Mature: 63185

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVEAAGNEQLVRVVVLLAAGVVAVPLFKRLGLGSVLGYLAAGLAIGPFGLGLFVDAQTI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LHVAELGVVMFLFVIGLEMQPKRLWSLRREIFGLGLAQVVACGALLTGVGMLAGLSAPVA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
FVAAMGFVMTSTATVMLILDERGETATSRGQRVVSILLLEDLAIVPLLAIVAFLAPAHAD
HHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VDSSIWRALLLALVAVGSVTIAGRWLLNPMFRLLAAARAREVMTAAALLVVLGAALLMQF
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGLSMAMGAFLAGVMLSESSFRHQLEADIEPFRGILLGLFFLSVGMSLDLTVFASHWPQV
CCHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LGGVVVFMLVKAAGIYVVARALGAPHGDALHRMVLMAQGGEFAFVLYSAGVSAGLLDGPM
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHH
NAVLTAIVILSMALTPLAVGALRWMLPRGAASLEGVDRADGLTGSVLMIGFGRFGQVASQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHH
ALLARGVDVAIIDSDTEMIQSADGFGFKVYYGDGTRLDVLHASGAATAEAIAVCVDDKAA
HHHHCCCCEEEECCCHHHHHCCCCCEEEEEECCCCEEEEEECCCCCHHHHEEEEECCHHH
ALKIVELVKHSFPQARLLVRSFDREHALKLIAAGVDYQVRETFESASAFGEAALRALGAT
HHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
PDEAAQVAAEVRRRDDERLQLQITGGLRAGVGMMLSNRMKPTPLTKPARAGLALNEETAS
CHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCCCCCCCCCHHCCCEECCCCCC
VTANAP
EECCCC
>Mature Secondary Structure 
AVEAAGNEQLVRVVVLLAAGVVAVPLFKRLGLGSVLGYLAAGLAIGPFGLGLFVDAQTI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LHVAELGVVMFLFVIGLEMQPKRLWSLRREIFGLGLAQVVACGALLTGVGMLAGLSAPVA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
FVAAMGFVMTSTATVMLILDERGETATSRGQRVVSILLLEDLAIVPLLAIVAFLAPAHAD
HHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VDSSIWRALLLALVAVGSVTIAGRWLLNPMFRLLAAARAREVMTAAALLVVLGAALLMQF
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGLSMAMGAFLAGVMLSESSFRHQLEADIEPFRGILLGLFFLSVGMSLDLTVFASHWPQV
CCHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LGGVVVFMLVKAAGIYVVARALGAPHGDALHRMVLMAQGGEFAFVLYSAGVSAGLLDGPM
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHH
NAVLTAIVILSMALTPLAVGALRWMLPRGAASLEGVDRADGLTGSVLMIGFGRFGQVASQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHH
ALLARGVDVAIIDSDTEMIQSADGFGFKVYYGDGTRLDVLHASGAATAEAIAVCVDDKAA
HHHHCCCCEEEECCCHHHHHCCCCCEEEEEECCCCEEEEEECCCCCHHHHEEEEECCHHH
ALKIVELVKHSFPQARLLVRSFDREHALKLIAAGVDYQVRETFESASAFGEAALRALGAT
HHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
PDEAAQVAAEVRRRDDERLQLQITGGLRAGVGMMLSNRMKPTPLTKPARAGLALNEETAS
CHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCCCCCCCCCHHCCCEECCCCCC
VTANAP
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]