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The map label for this gene is 124028464

Identifier: 124028464

GI number: 124028464

Start: 1623451

End: 1625118

Strand: Direct

Name: 124028464

Synonym: Hbut_1624

Alternate gene names: NA

Gene position: 1623451-1625118 (Clockwise)

Preceding gene: 124028463

Following gene: 124028465

Centisome position: 97.38

GC content: 55.82

Gene sequence:

>1668_bases
TTGAAGCTTGACACTGCTACCGTTGAAGTTATTAGGAATGCTTCGATATACATTGCCGAGGAGATGGGTGTTGTGCTGAG
GAATACAGCGCTAAGCCCCAACATACGCGACCGTCTAGACCATTCATGCGCCATACTCGACGCTGAAGGCAATCTTGTAG
CGCAAGCCGAGCACATACCAGTACATCTCGGCAGCATGTCGGTTGCAGCAAGGAATATCGTCGAGTGGCTCGAACGCAAC
GGTGTCACACTCGACGAGGGCGATGTAGTAGTCTCCAACGACCCATACATAGTCGGCACACATTTGAACGACTTGATGCT
CCTCAAGCCAGTCTACGCTGAAGGCAGGCTACTGGGCTACGTGGCTGCTAAGGCCCACCACGTCGATATCGGGGGAGGTG
TACCCGGGAGCCTAAGCCCAAATGCGAAGAGCCTCATAGAAGAGGGGCTTGTCATACCCCCCGTCAAGCTCGTGGTGAGG
GGCGAGCTTAGGAGGGATATCCTGCGTCTCCTCGAGGCTAACGTGCGTACCCCACGGTATCTCCGCGGCGACCTCCTTGC
CCAGATTGCAGCTGTCAACGTGGGGGAGCGAAGGCTCAGGGAACTAGTAGACCGGTATGGCGCCAGTATCGTGATGGAGG
CGTGGAAGAGGACGCTGGACTATACGGAGAGGTATGCAAGGAACCGTATACGAGCCCTTGTAGAGAAATATGGCGTTTAT
GGCAAGGTGTTTACCGCCGAGGACTACCTGGAGCTGGGGAGCGGTCCCGCGCGGATAAGGGTCTCGATACTGTTTAGTGG
TGGCAGCATAACCGTGGATTTTGCTGGCTCGAACCGCCAAGTTGATGAACCGTTTAACGCAGTATATGGGGTAACTGTTG
CAGCTACAACGTTCGCACTGAAGGCCGTTATTGACCCGGAAATGCCTATGAATCAAGGGTTTTACCGTGTTGTAAACATA
GCTGCGCCTAGGGGGACTATAGTTAACCCGGAGCCGCCAGCGCCGGTTGGAGGTGGCAATGTTGAGACCTCGCAGAGGAT
TGTAGATGTTGTGTTGAGGGCTCTCGCGGATGCTTTCCCGGACCGGGTGCCCGCGGCAAGCTGTGGCACCATGACTAACG
TGGCTCTCGGTGGTAGGGGATGGGCTTTCTACGAGACTGTGGGATGCGGTAGCGGCGCTAGACCATGCTGCGATGGAGTT
GACGGCGTACACACCAACATGACTAACACTCTTAACACACCGATAGAGGTTGCTGAGGCTGAGTACCCGATACTCTTCCT
AGCTTACGAGCTTCGCCCCAACTCAGGCGGATCCGGCAAGTATAGGGGCGGCCTTGGGATTATACGGGCATTTACAGTGC
TCGAGGACGGTGTTAAGCTTAGCATCATAGGTGAACGTGTTAGGCTTAGGCCTTGGGGGCTTCGGGGCGGAGGCAAAGGC
GCCCCCGCGAGGTTTATCATTGTACGGCGTGATGGACGTGTAGAGCTGTTGGAGTCTAAGGCTAGTGTTGAGCTGCATAG
AGGCGATCGTGTCTACATAGCTACGCCAGGCGGAGGCGGCTATGGCGACTCTTGCAGCCGTGACCGCGAGCTAGTTAGGC
GTGATCTCGAGGACGGCAAGATAACCCAGTGGCACGCCAGGAGGTACTATTGCTACGAGACTGAGTAG

Upstream 100 bases:

>100_bases
GTTATAGAGTCTGATGATAGCACCATACTAATTCCACCAGACAGTGTTGGCACAGTAGACGAGCACCATTCCATAGTTAT
TAGCGTTGGGGGTGGCAGTG

Downstream 100 bases:

>100_bases
ACCATGGCTGAAAGATTCGCCCGAGCAACCCCCACTGGCTACATCTTACTGCTATAACACACGTTTTAGCTGCTACCATG
TCTTGCCACCGCTCGAGACT

Product: Hydantoin utilization protein B

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: NA

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MKLDTATVEVIRNASIYIAEEMGVVLRNTALSPNIRDRLDHSCAILDAEGNLVAQAEHIPVHLGSMSVAARNIVEWLERN
GVTLDEGDVVVSNDPYIVGTHLNDLMLLKPVYAEGRLLGYVAAKAHHVDIGGGVPGSLSPNAKSLIEEGLVIPPVKLVVR
GELRRDILRLLEANVRTPRYLRGDLLAQIAAVNVGERRLRELVDRYGASIVMEAWKRTLDYTERYARNRIRALVEKYGVY
GKVFTAEDYLELGSGPARIRVSILFSGGSITVDFAGSNRQVDEPFNAVYGVTVAATTFALKAVIDPEMPMNQGFYRVVNI
AAPRGTIVNPEPPAPVGGGNVETSQRIVDVVLRALADAFPDRVPAASCGTMTNVALGGRGWAFYETVGCGSGARPCCDGV
DGVHTNMTNTLNTPIEVAEAEYPILFLAYELRPNSGGSGKYRGGLGIIRAFTVLEDGVKLSIIGERVRLRPWGLRGGGKG
APARFIIVRRDGRVELLESKASVELHRGDRVYIATPGGGGYGDSCSRDRELVRRDLEDGKITQWHARRYYCYETE

Sequences:

>Translated_555_residues
MKLDTATVEVIRNASIYIAEEMGVVLRNTALSPNIRDRLDHSCAILDAEGNLVAQAEHIPVHLGSMSVAARNIVEWLERN
GVTLDEGDVVVSNDPYIVGTHLNDLMLLKPVYAEGRLLGYVAAKAHHVDIGGGVPGSLSPNAKSLIEEGLVIPPVKLVVR
GELRRDILRLLEANVRTPRYLRGDLLAQIAAVNVGERRLRELVDRYGASIVMEAWKRTLDYTERYARNRIRALVEKYGVY
GKVFTAEDYLELGSGPARIRVSILFSGGSITVDFAGSNRQVDEPFNAVYGVTVAATTFALKAVIDPEMPMNQGFYRVVNI
AAPRGTIVNPEPPAPVGGGNVETSQRIVDVVLRALADAFPDRVPAASCGTMTNVALGGRGWAFYETVGCGSGARPCCDGV
DGVHTNMTNTLNTPIEVAEAEYPILFLAYELRPNSGGSGKYRGGLGIIRAFTVLEDGVKLSIIGERVRLRPWGLRGGGKG
APARFIIVRRDGRVELLESKASVELHRGDRVYIATPGGGGYGDSCSRDRELVRRDLEDGKITQWHARRYYCYETE
>Mature_555_residues
MKLDTATVEVIRNASIYIAEEMGVVLRNTALSPNIRDRLDHSCAILDAEGNLVAQAEHIPVHLGSMSVAARNIVEWLERN
GVTLDEGDVVVSNDPYIVGTHLNDLMLLKPVYAEGRLLGYVAAKAHHVDIGGGVPGSLSPNAKSLIEEGLVIPPVKLVVR
GELRRDILRLLEANVRTPRYLRGDLLAQIAAVNVGERRLRELVDRYGASIVMEAWKRTLDYTERYARNRIRALVEKYGVY
GKVFTAEDYLELGSGPARIRVSILFSGGSITVDFAGSNRQVDEPFNAVYGVTVAATTFALKAVIDPEMPMNQGFYRVVNI
AAPRGTIVNPEPPAPVGGGNVETSQRIVDVVLRALADAFPDRVPAASCGTMTNVALGGRGWAFYETVGCGSGARPCCDGV
DGVHTNMTNTLNTPIEVAEAEYPILFLAYELRPNSGGSGKYRGGLGIIRAFTVLEDGVKLSIIGERVRLRPWGLRGGGKG
APARFIIVRRDGRVELLESKASVELHRGDRVYIATPGGGGYGDSCSRDRELVRRDLEDGKITQWHARRYYCYETE

Specific function: Unknown

COG id: COG0146

COG function: function code EQ; N-methylhydantoinase B/acetone carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=535, Percent_Identity=39.8130841121495, Blast_Score=324, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI133901900, Length=539, Percent_Identity=35.8070500927644, Blast_Score=305, Evalue=5e-83,
Organism=Caenorhabditis elegans, GI133901902, Length=507, Percent_Identity=35.5029585798817, Blast_Score=280, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6322634, Length=554, Percent_Identity=34.6570397111913, Blast_Score=290, Evalue=3e-79,
Organism=Drosophila melanogaster, GI45550492, Length=568, Percent_Identity=36.6197183098592, Blast_Score=317, Evalue=1e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003692 [H]

Pfam domain/function: PF02538 Hydantoinase_B [H]

EC number: 3.5.2.14

Molecular weight: Translated: 60467; Mature: 60467

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDTATVEVIRNASIYIAEEMGVVLRNTALSPNIRDRLDHSCAILDAEGNLVAQAEHIP
CCCCHHHHHHHHCCEEEEEECCCEEEECCCCCCCHHHHCCCCEEEEECCCCEEEEECCCE
VHLGSMSVAARNIVEWLERNGVTLDEGDVVVSNDPYIVGTHLNDLMLLKPVYAEGRLLGY
EEECCHHHHHHHHHHHHHHCCCEECCCCEEECCCCEEEECCCCCEEEEEECCCCCEEEEE
VAAKAHHVDIGGGVPGSLSPNAKSLIEEGLVIPPVKLVVRGELRRDILRLLEANVRTPRY
EEEEEEEEECCCCCCCCCCCCHHHHHHCCCCCCCHHEEEEHHHHHHHHHHHHCCCCCCHH
LRGDLLAQIAAVNVGERRLRELVDRYGASIVMEAWKRTLDYTERYARNRIRALVEKYGVY
HHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKVFTAEDYLELGSGPARIRVSILFSGGSITVDFAGSNRQVDEPFNAVYGVTVAATTFAL
EEEEECHHHHHHCCCCCEEEEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
KAVIDPEMPMNQGFYRVVNIAAPRGTIVNPEPPAPVGGGNVETSQRIVDVVLRALADAFP
HHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
DRVPAASCGTMTNVALGGRGWAFYETVGCGSGARPCCDGVDGVHTNMTNTLNTPIEVAEA
CCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCC
EYPILFLAYELRPNSGGSGKYRGGLGIIRAFTVLEDGVKLSIIGERVRLRPWGLRGGGKG
CCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCEEEEEECCCCCCCCC
APARFIIVRRDGRVELLESKASVELHRGDRVYIATPGGGGYGDSCSRDRELVRRDLEDGK
CCEEEEEEEECCCEEEEECCCCEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCC
ITQWHARRYYCYETE
EEEEECCEEEEEECC
>Mature Secondary Structure
MKLDTATVEVIRNASIYIAEEMGVVLRNTALSPNIRDRLDHSCAILDAEGNLVAQAEHIP
CCCCHHHHHHHHCCEEEEEECCCEEEECCCCCCCHHHHCCCCEEEEECCCCEEEEECCCE
VHLGSMSVAARNIVEWLERNGVTLDEGDVVVSNDPYIVGTHLNDLMLLKPVYAEGRLLGY
EEECCHHHHHHHHHHHHHHCCCEECCCCEEECCCCEEEECCCCCEEEEEECCCCCEEEEE
VAAKAHHVDIGGGVPGSLSPNAKSLIEEGLVIPPVKLVVRGELRRDILRLLEANVRTPRY
EEEEEEEEECCCCCCCCCCCCHHHHHHCCCCCCCHHEEEEHHHHHHHHHHHHCCCCCCHH
LRGDLLAQIAAVNVGERRLRELVDRYGASIVMEAWKRTLDYTERYARNRIRALVEKYGVY
HHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKVFTAEDYLELGSGPARIRVSILFSGGSITVDFAGSNRQVDEPFNAVYGVTVAATTFAL
EEEEECHHHHHHCCCCCEEEEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
KAVIDPEMPMNQGFYRVVNIAAPRGTIVNPEPPAPVGGGNVETSQRIVDVVLRALADAFP
HHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
DRVPAASCGTMTNVALGGRGWAFYETVGCGSGARPCCDGVDGVHTNMTNTLNTPIEVAEA
CCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCC
EYPILFLAYELRPNSGGSGKYRGGLGIIRAFTVLEDGVKLSIIGERVRLRPWGLRGGGKG
CCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCEEEEEECCCCCCCCC
APARFIIVRRDGRVELLESKASVELHRGDRVYIATPGGGGYGDSCSRDRELVRRDLEDGK
CCEEEEEEEECCCEEEEECCCCEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCC
ITQWHARRYYCYETE
EEEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]